THE INCREASE IN PLASMINOGEN ACTIVAT OR INHIBITOR TYPE-1EXPRESSION BY STIM ULATION OF ACTIVATORS FOR PEROXISOME PROLIFER ATOR-ACTIVATED RECEPTORS IN HUMAN ENDOTH ELIAL CELLS

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THE INCREASE IN PLASMINOGEN ACTIVAT OR INHIBITOR TYPE-1EXPRESSION BY STIM ULATION OF ACTIVATORS FOR PEROXISOME PROLIFER ATOR-ACTIVATED RECEPTORS IN HUMAN ENDOTH ELIAL CELLS

Chinese Medical Sciences Journal Vol. 0, Issue 2, Pages: 112-116(2002)

Updated：2024-04-10

- THE INCREASE IN PLASMINOGEN ACTIVAT OR INHIBITOR TYPE-1EXPRESSION BY STIM ULATION OF ACTIVATORS FOR PEROXISOME PROLIFER ATOR-ACTIVATED RECEPTORS IN HUMAN ENDOTH ELIAL CELLS
- Affiliations：
- Author bio：
- Funds：
  
  ThisworkwassupportedbytheNationalNaturalSciencesFoundationofChina（39970301）.
- DOI：
  CLC： R540.2
- Published：2002
- Accepted：
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THE INCREASE IN PLASMINOGEN ACTIVAT OR INHIBITOR TYPE-1EXPRESSION BY STIM ULATION OF ACTIVATORS FOR PEROXISOME PROLIFER ATOR-ACTIVATED RECEPTORS IN HUMAN ENDOTH ELIAL CELLS[J]. Chinese medical sciences journal, 2002, 0(2): 112-116.
DOI：

THE INCREASE IN PLASMINOGEN ACTIVAT OR INHIBITOR TYPE-1EXPRESSION BY STIM ULATION OF ACTIVATORS FOR PEROXISOME PROLIFER ATOR-ACTIVATED RECEPTORS IN HUMAN ENDOTH ELIAL CELLS[J]. Chinese medical sciences journal, 2002, 0(2): 112-116. DOI：

摘要

Abstract

Objective.To investigate the effect of peroxis ome proliferator-activated recept ors（PPARs ）activators on plasminogen activator inhibitor ty pe-1（PAI-1）expression in human umbilical vein e ndothelial cells and the possi-ble mechanism.Methods.Human umbilical vein endothelial ce lls（HUVECs ）were obtained from normal fetus

and cul-tured conventionally.Then the HUVECs were exposed to test agents（linolenic acid

linoleic acid

oleic acid

stearic acid and prostaglandin J 2 respectively）in varying concentrations with fresh media.RT -PCR and ELISA were applied to determine the expression of PPARs and PAI-1in HUVECs.Results.PPARα

PPARδand PPARγmRNA were detected by using RT-PCR in HUVECs.Treatment of HUVECs with PPARαand PPARγactivators---linolenic acid

linoleic acid

oleic acid and prostaglandin J 2 respectively

but not with stearic a cid could augment PAI-I mRNA expression and protein secretion in a concentration-dependent manner.However

the mRNA expressions of 3subclasses of PPAR with their activators in HUVECs were not changed compared w ith controls.Conclusion.HUVECs express PPARs.PPARs activators may increase PAI-1expression in ECs

but the underlying mechanism remains uncle ar.Although PPARs expression was not enhanced after stimulated by their activators in ECs

the role of functionally active PPARs in regulating PA I-1expression in ECs needs to be further investigated by using transient gen e transfection assay.

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