PURIFICATION AND IDENTIFICATION OF THE ANTIGEN ASSOCIATED WITH MONOCLONAL ANTIBODY 3H<sub>11</sub> AGAINST GASTRIC CANCER CELL

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PURIFICATION AND IDENTIFICATION OF THE ANTIGEN ASSOCIATED WITH MONOCLONAL ANTIBODY 3H₁₁ AGAINST GASTRIC CANCER CELL

Chinese Medical Sciences Journal Vol. 0, Issue 3, Pages: 150-153(1998)

Updated：2024-04-10

- PURIFICATION AND IDENTIFICATION OF THE ANTIGEN ASSOCIATED WITH MONOCLONAL ANTIBODY 3H₁₁ AGAINST GASTRIC CANCER CELL
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- DOI：
  CLC： R735.2
- Published：1998
- Accepted：
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PURIFICATION AND IDENTIFICATION OF THE ANTIGEN ASSOCIATED WITH MONOCLONAL ANTIBODY 3H₁₁ AGAINST GASTRIC CANCER CELL[J]. Chinese medical sciences journal, 1998, 0(3): 150-153.
DOI：

PURIFICATION AND IDENTIFICATION OF THE ANTIGEN ASSOCIATED WITH MONOCLONAL ANTIBODY 3H₁₁ AGAINST GASTRIC CANCER CELL[J]. Chinese medical sciences journal, 1998, 0(3): 150-153. DOI：

摘要

Abstract

The object of this study is to investigate the properties of gastric cancer assoiciated antigen by McAb3H

against gastric cancer cell line. Antigen purified by affinity chromatography and further charac- terized by biochemical and immunological techiques. Results showed that assoiciated antigen of McAb 3H

was mainly located on the membrane of target cells. It was sensitive to heat and digestion of proteases

but resistant to treatment with sodium periodate. Schiff’s reagent staining was negative. SDS-PAGE and IEF- analysis showed that the antigen was a 210 kD molecular weight with pl of 8. 5 protein. The molecular weight of antigen extracted from target cell with tunicamycin was not changed. We concluded that the cor- responding antigen of McAb 3H

might be an alkaline protein.

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Related Author

王俊梅

张波

杨邵敏

韩继生

李冰思

侯琳

Related Institution

Department of Pathology

Health Science Center of Peking University

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PURIFICATION AND IDENTIFICATION OF THE ANTIGEN ASSOCIATED WITH MONOCLONAL ANTIBODY 3H11 AGAINST GASTRIC CANCER CELL

DOI：

摘要

Abstract

关键词

Keywords

references

PURIFICATION AND IDENTIFICATION OF THE ANTIGEN ASSOCIATED WITH MONOCLONAL ANTIBODY 3H₁₁ AGAINST GASTRIC CANCER CELL