TANDEM REPEATING OF THE EXPRESSIONCARTRIDGE──A NOVEL STRATEGY TOENHANCE THE EXPRESSION EFFICIENCY OFCLONED GENE IN ESCHERICHIA COLI

Lin Ying and Lu Shengdong

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TANDEM REPEATING OF THE EXPRESSIONCARTRIDGE──A NOVEL STRATEGY TOENHANCE THE EXPRESSION EFFICIENCY OFCLONED GENE IN ESCHERICHIA COLI

Chinese Medical Sciences Journal Issue 4, (1996)

Updated：2024-04-10

- TANDEM REPEATING OF THE EXPRESSIONCARTRIDGE──A NOVEL STRATEGY TOENHANCE THE EXPRESSION EFFICIENCY OFCLONED GENE IN ESCHERICHIA COLI
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  CLC： R363
- Published：1996
- Accepted：
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[1]林缨,卢圣栋.TANDEM　REPEATING　OF　THE　EXPRESSION　CARTRIDGE──A　NOVEL　STRATEGY　TO　ENHANCE　THE　EXPRESSION　EFFICIENCY　OF　CLONED　GENE　IN　ESCHERI[J].Chinese Medical Sciences Journal,1996(04):204-208.

Lin Ying and Lu Shengdong. TANDEM REPEATING OF THE EXPRESSIONCARTRIDGE──A NOVEL STRATEGY TOENHANCE THE EXPRESSION EFFICIENCY OFCLONED GENE IN ESCHERICHIA COLI[J]. Chinese medical sciences journal, 1996, (4).
[1]林缨,卢圣栋.TANDEM　REPEATING　OF　THE　EXPRESSION　CARTRIDGE──A　NOVEL　STRATEGY　TO　ENHANCE　THE　EXPRESSION　EFFICIENCY　OF　CLONED　GENE　IN　ESCHERI[J].Chinese Medical Sciences Journal,1996(04):204-208. DOI：

Lin Ying and Lu Shengdong. TANDEM REPEATING OF THE EXPRESSIONCARTRIDGE──A NOVEL STRATEGY TOENHANCE THE EXPRESSION EFFICIENCY OFCLONED GENE IN ESCHERICHIA COLI[J]. Chinese medical sciences journal, 1996, (4). DOI：

摘要

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Abstract

A novel strategy to enhance the expression efficiency of cloned target gene in Escherichia coli was developed. The whole expression cartridge

consisting of promoter. SD sequence

target gene and transcription terminator

was tandem repeatedly engineered into a expression plasmid. Consequently

the copy number of specific gene was increased substantially

leading to the improvement of expression efficiency.Using this approach

a recombinant plasmid

designed as PLYD

was constructed and transformated into the Escherichia coli strain DH5α. Upon induction

the desired protein was synthesized in a considerable level and accumulated up to 63% of the total cell proteins. The present study revealed that tandem repeating of expression cartridge provided a convenient means to improve expression level efficiently.

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