SENSITIZATION OF ACNU KILLING EFFECTS ON HeLa S3 CELLS BY MGMT ANTISENSE RNA TRANSFECTION

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SENSITIZATION OF ACNU KILLING EFFECTS ON HeLa S3 CELLS BY MGMT ANTISENSE RNA TRANSFECTION

Chinese Medical Sciences Journal Vol. 0, Issue 1, Pages: 14-19(1998)

Updated：2024-04-10

- SENSITIZATION OF ACNU KILLING EFFECTS ON HeLa S3 CELLS BY MGMT ANTISENSE RNA TRANSFECTION
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  CLC： R730.2
- Published：1998
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SENSITIZATION OF ACNU KILLING EFFECTS ON HeLa S3 CELLS BY MGMT ANTISENSE RNA TRANSFECTION[J]. Chinese medical sciences journal, 1998, 0(1): 14-19.
DOI：

SENSITIZATION OF ACNU KILLING EFFECTS ON HeLa S3 CELLS BY MGMT ANTISENSE RNA TRANSFECTION[J]. Chinese medical sciences journal, 1998, 0(1): 14-19. DOI：

摘要

Abstract

O6 -methylguanine- DNA- methyltransferase （MGMT ） plays a very important role in the cellular resis- tance to nitrosoureas drugs. Inhibition of MGMT might be a useful approach in tumor chemotherapy. In this study

the depletion of MGMT activity by retroviral-mediated antisense RNA transfection were reported. Three retroviral vectors expressing MGMT antisense RNA were constructed and transfected into HeLa S3 cells. The difference of MGMT mRNA

MGMT activity as well as cellular resistance to ACNU before and after transfection were observed. It was found that antisense RNA targeting 5’region and whole length of MGMT mRNA could partially deplete MGMT activity and enhance killing effects of ACNU. However

3’ region antisense RNA had no effect on MGMT modulation.

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