SPECIFIC BINDING OF HUMAN BONE MORPHOGENETIC PROTEIN （2A） WITH MOUSE OSTEOBLASTIC CELLS

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SPECIFIC BINDING OF HUMAN BONE MORPHOGENETIC PROTEIN （2A） WITH MOUSE OSTEOBLASTIC CELLS

Chinese Medical Sciences Journal Issue 2, (1996)

Updated：2024-04-10

- SPECIFIC BINDING OF HUMAN BONE MORPHOGENETIC PROTEIN （2A） WITH MOUSE OSTEOBLASTIC CELLS
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  CLC： R329-34
- Published：1996
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[1]刘新平,陈苏民,陈南春,高磊,赵忠良.SPECIFIC　BINDING　OF　HUMAN　BONEMORPHOGENETIC　PROTEIN　（2A）　WITH　MOUSEOSTEOBLASTIC　CELLS[J].Chinese Medical Sciences Journal,1996(02):97-99.

SPECIFIC BINDING OF HUMAN BONE MORPHOGENETIC PROTEIN （2A） WITH MOUSE OSTEOBLASTIC CELLS[J]. Chinese medical sciences journal, 1996, (2).
[1]刘新平,陈苏民,陈南春,高磊,赵忠良.SPECIFIC　BINDING　OF　HUMAN　BONEMORPHOGENETIC　PROTEIN　（2A）　WITH　MOUSEOSTEOBLASTIC　CELLS[J].Chinese Medical Sciences Journal,1996(02):97-99. DOI：

SPECIFIC BINDING OF HUMAN BONE MORPHOGENETIC PROTEIN （2A） WITH MOUSE OSTEOBLASTIC CELLS[J]. Chinese medical sciences journal, 1996, (2). DOI：

摘要

ＳＰＥＣＩＦＩＣＢＩＮＤＩＮＧＯＦＨＵＭＡＮＢＯＮＥＭＯＲＰＨＯＧＥＮＥＴＩＣＰＲＯＴＥＩＮ（２Ａ）ＷＩＴＨＭＯＵＳＥＯＳＴＥＯＢＬＡＳＴＩＣＣＥＬＬＳＬｉｕＸｉｎｐｉｎｇ；（刘新平），ＣｈｅｎＳｕｍｉｎ；（陈苏民），ＣｈｅｎＮａｎｃｈｕｎ；(陈南春...

Abstract

Human bone morphogenetic protein 2A （hBMP2A） cDNA terminal 567 nucleotides were cloned and expressed in a phage display vector pCSM21. Human BMP2A C-terminal peptide displayed on the surface of the phage can bind specifically to the surface of mouse osteoblastic cell （MC3T3） membrane. ELISA assay showed a positive signal of the binding by using antibody against M13 phage gene 8 protein. After labeling with 3HTdR

the counts of the binding groups were 3 to 10 times higher than the control groups. It suggests that the surface of MC3T3 cells exist the receptor for hBMP2A.

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