Glycoproteins from tumor cells isolated by Con A

PHAE and RCAI

were coupled to horse radish peroxidase and then used as reporters to evaluate the oligosaccharides change on serum glycoproteins in digestive tract diseases and individual healthy persons. A value 2 standard deviation above the mean of the healthy person group was considered positive. The results indicated that the positive rates of the oligosaccharides bound with Con A

PHA-E and RCAI were 51 % （61/119） . 70. 6% （84/119）

and 76. 4% （91/119） in patients with gastric cancer: 53. 3% （16/30）

46. 7% （ 14/30） and 66. 7% （20/30） in esophageal cancers; 28. 5% （15/49）

49. 0% （24/49） and .46. 9% （23/49） in colorectal cancer; 78. 1% （25/ 32）

81. 3% （26/32） and 31. 3% （10/32） in gallbladder tumors; 60. 4% （29/48）

54. 1% （26/48） and 39. 5% （19/48） in hepatocellular cancer. The positive rates among the patients with benign diseases were also found to be 12. 7% （16/126）

15. 5% （20/126） and 26. 2% （33/126）. However

the expression of the oligosaccharide moieties recognized by WGA on the glycoprotein bound with Con A

was significantly lower in the serum of patients with tumors than in those with benign disease and in healthy individuals （P<0. 01

P<0. 01）.