COMPARISON OF FOUR METHODS TO GENERATE IMMUNOREACTIVE FRAGMENTS OF A MURINE MONOCLONAL ANTIBODY OC859 AGAINST HUMAN OVARIAN EPITHELIAL CANCER ANTIGEN

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COMPARISON OF FOUR METHODS TO GENERATE IMMUNOREACTIVE FRAGMENTS OF A MURINE MONOCLONAL ANTIBODY OC859 AGAINST HUMAN OVARIAN EPITHELIAL CANCER ANTIGEN

Chinese Medical Sciences Journal Issue 2, (1995)

Updated：2024-04-10

- COMPARISON OF FOUR METHODS TO GENERATE IMMUNOREACTIVE FRAGMENTS OF A MURINE MONOCLONAL ANTIBODY OC859 AGAINST HUMAN OVARIAN EPITHELIAL CANCER ANTIGEN
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  CLC： R363
- Published：1995
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[1]邹颖,卞美璐,杨子义,连利娟,刘文淑,许秀英.COMPARISON　OF　FOUR　METHODS　TO　GENERATE　IMMUNOREACTIVE　FRAGMENTS　OF　A　MURINE　MONOCLONAL　ANTIBODY　OC859　AGAINST　HUMAN　OVARIAN　E[J].Chinese Medical Sciences Journal,1995(02):78-81.

COMPARISON OF FOUR METHODS TO GENERATE IMMUNOREACTIVE FRAGMENTS OF A MURINE MONOCLONAL ANTIBODY OC859 AGAINST HUMAN OVARIAN EPITHELIAL CANCER ANTIGEN[J]. Chinese medical sciences journal, 1995, (2).
[1]邹颖,卞美璐,杨子义,连利娟,刘文淑,许秀英.COMPARISON　OF　FOUR　METHODS　TO　GENERATE　IMMUNOREACTIVE　FRAGMENTS　OF　A　MURINE　MONOCLONAL　ANTIBODY　OC859　AGAINST　HUMAN　OVARIAN　E[J].Chinese Medical Sciences Journal,1995(02):78-81. DOI：

COMPARISON OF FOUR METHODS TO GENERATE IMMUNOREACTIVE FRAGMENTS OF A MURINE MONOCLONAL ANTIBODY OC859 AGAINST HUMAN OVARIAN EPITHELIAL CANCER ANTIGEN[J]. Chinese medical sciences journal, 1995, (2). DOI：

摘要

ＣＯＭＰＡＲＩＳＯＮＯＦＦＯＵＲＭＥＴＨＯＤＳＴＯＧＥＮＥＲＡＴＥＩＭＭＵＮＯＲＥＡＣＴＩＶＥＦＲＡＧＭＥＮＴＳＯＦＡＭＵＲＩＮＥＭＯＮＯＣＬＯＮＡＬ　ＡＮＴＩＢＯＤＹＯＣ８５９ＡＧＡＩＮＳＴＨＵＭＡＮＯＶＡＲＩＡＮＥＰＩＴＨＥＬＩＡＬＣＡＮＣＥＲＡ...

Abstract

In the present study

four different proteases （pepsin

papain

bromelain and ficin） were screened with a murine monoclonal antibody OC859

in order to verify whether different digestion procedures could improve yield and stability of the F（ab’）2 or Fab fragments. The yields of F（ab’）2 or Fab fragments from digestion with pepsin

papain

bromelain and ficin were respectively 20. 3+/-2. 0%

50. 5+/-5. 0%

74. 4+/-2. 7% and 82. 8 +/-10. 2% of the theoretical maximum. Immunoreactivity in a noncompetitive solid-phase radioimmunoassay （SPRIA） of the fragments generated by the four proteases were respectively 10+/-5%

36+/-5%

60+/-6% and 75+/-6%of the intact OC859 IgG. These results suggested that the fragmentation of OC859 with ficin gave a higher yield of superior immunoreactive fragments.

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