HIGH DENSITY CULTIVATION OF A RECOMBINANT CD-1 CELL LINE PRODUCING PROUROKINASE USING A BIOSILON MICROCARRIER CULTURE SYSTEM

Xiao Chengzu; Huang Zicai; Zhang Zhengguang Ye Jianxin; Gao Lihua; Guo Zhixia; Cheng DushengZhou Heshan and Kong Weiwei

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HIGH DENSITY CULTIVATION OF A RECOMBINANT CD-1 CELL LINE PRODUCING PROUROKINASE USING A BIOSILON MICROCARRIER CULTURE SYSTEM

Chinese Medical Sciences Journal Issue 4, (1994)

Updated：2024-04-10

- HIGH DENSITY CULTIVATION OF A RECOMBINANT CD-1 CELL LINE PRODUCING PROUROKINASE USING A BIOSILON MICROCARRIER CULTURE SYSTEM
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  CLC： R363
- Published：1994
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[1]肖成祖,黄子才,张正光,叶建新,高丽华,郭智霞,程度胜,周鹤山,孔惟惟.HIGH　DENSITY　CULTIVATION　OF　A　RECOMBINANT　CD－1　CELL　LINE　PRODUCING　PROUROKINASE　USING　A　BIOSILON　MICROCARRIER　CULTURE　SYSTEM[J].Chinese Medical Sciences Journal,1994(04):203-208.

Xiao Chengzu, Huang Zicai, Zhang Zhengguang Ye Jianxin, et al. HIGH DENSITY CULTIVATION OF A RECOMBINANT CD-1 CELL LINE PRODUCING PROUROKINASE USING A BIOSILON MICROCARRIER CULTURE SYSTEM[J]. Chinese medical sciences journal, 1994, (4).
[1]肖成祖,黄子才,张正光,叶建新,高丽华,郭智霞,程度胜,周鹤山,孔惟惟.HIGH　DENSITY　CULTIVATION　OF　A　RECOMBINANT　CD－1　CELL　LINE　PRODUCING　PROUROKINASE　USING　A　BIOSILON　MICROCARRIER　CULTURE　SYSTEM[J].Chinese Medical Sciences Journal,1994(04):203-208. DOI：

Xiao Chengzu, Huang Zicai, Zhang Zhengguang Ye Jianxin, et al. HIGH DENSITY CULTIVATION OF A RECOMBINANT CD-1 CELL LINE PRODUCING PROUROKINASE USING A BIOSILON MICROCARRIER CULTURE SYSTEM[J]. Chinese medical sciences journal, 1994, (4). DOI：

摘要

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Abstract

CD-1

a genetically-engineered CHO cell line

was cultivated with a Biosilon microcarrier culture system.We successfully cultivated CD-1 cells to a very high density （over1×107cells/ml）. Prourokinase was stably secreted at about 180 IU/ 1e6 cells/24 h. Experiments showed that CD-1 cells growing on Biosilon microcarriers were able to spontaneously release from the microcarriers

then reatthch and proliferate on fresh microcarriers. This makes it very easy to scale up preduction. The microcarriers could be reused several times without affecting adhesion. proliferation and prourokinase secretion. With CMPECC membrane radial flow chromatography and MPG chromatography

the prourokinase in conditioned medium could be purified to a specific activity of 1×105 IU/mg of protein. The purification factor was about 600 fold

and approxiamately 90 % of the biological activity was recovered.

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