CLONING AND IDENTIFICATION OF A RABBIT SPERM MEMBRANE PROTEIN GENE

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CLONING AND IDENTIFICATION OF A RABBIT SPERM MEMBRANE PROTEIN GENE

Chinese Medical Sciences Journal Issue 1, Pages: 4-8(1990)

Updated：2024-04-10

- CLONING AND IDENTIFICATION OF A RABBIT SPERM MEMBRANE PROTEIN GENE
- Affiliations：
  
  1. Center for Biomedical Research,The Popula tion Council.,New York
  2. ,China
  3. Shanghai Institute of Cell Biology
  4. Center for Biomedical Research
  5. The Population Council New York
- Author bio：
- Funds：
  
  This work was supported by the Rockefeller Foundation,NIH Grant and the Mellon Foundation,New York
- DOI：
  CLC：
- Published：1990
- Accepted：
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[1]王琳芳,缪时英,严缘昌,王维,刘强远,白云,朱红,S.Koide,Chen Chinling,C.W.Bardin.CLONING AND IDENTIFICATION OF A RABBIT SPERM MEMBRANE PROTEIN GENE[J].Chinese Medical Sciences Journal,1990(01):4-8.

CLONING AND IDENTIFICATION OF A RABBIT SPERM MEMBRANE PROTEIN GENE[J]. Chinese medical sciences journal, 1990, (1): 4-8.
[1]王琳芳,缪时英,严缘昌,王维,刘强远,白云,朱红,S.Koide,Chen Chinling,C.W.Bardin.CLONING AND IDENTIFICATION OF A RABBIT SPERM MEMBRANE PROTEIN GENE[J].Chinese Medical Sciences Journal,1990(01):4-8. DOI：

CLONING AND IDENTIFICATION OF A RABBIT SPERM MEMBRANE PROTEIN GENE[J]. Chinese medical sciences journal, 1990, (1): 4-8. DOI：

摘要

<正> Antibodies raised against rabbit sperm membrane protein（rSMP-B）interacted with a rattestis cytosolic protein with an estimated molecular weight of 70 kD.The clone expressingrSMP-B was isolated by epitope selection from a rat testis

λgt11 vector cDNA library

An Eco Rl-eut 2.0 kb cDNA fragment encoding rSMP-B was identified.The cDNA fragment（RSD-1）was isolated

subcloned and restriction mapped.Dot hybridization analysis of mRNAprepared from human

rabbit and rat testes showed that the gene encoding rSMP-B is ex-pressed in the testes of all three mammals.

Abstract

Antibodies raised against rabbit sperm membrane protein（rSMP-B）interacted with a rat testis cytosolic protein with an estimated molecular weight of 70 kD.The clone expressing rSMP-B was isolated by epitope selection from a rat testis

λgt11 vector cDNA library

An Eco Rl-eut 2.0 kb cDNA fragment encoding rSMP-B was identified.The cDNA fragment （RSD-1）was isolated

subcloned and restriction mapped.Dot hybridization analysis of mRNA prepared from human

rabbit and rat testes showed that the gene encoding rSMP-B is ex- pressed in the testes of all three mammals.

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