An Optimized Protocol of Azoxymethane-Dextran Sodium Sulfate Induced Colorectal Tumor Model in Mice

Liang Xi; Hu Jingnan; He Jianming

doi:10.24920/003495

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An Optimized Protocol of Azoxymethane-Dextran Sodium Sulfate Induced Colorectal Tumor Model in Mice

Chinese Medical Sciences Journal Vol. 34, Issue 4, Pages: 281-288(2019)

Technique Report | Updated：2024-04-10

- An Optimized Protocol of Azoxymethane-Dextran Sodium Sulfate Induced Colorectal Tumor Model in Mice
- Affiliations：
  
  Department of Radiotherapy, Hebei Province Hospital of Chinese Medicine, Hebei University of Chinese Medicine, Shijiazhuang 050011, China
- Author bio：
  
  # Authors contributed equally to this work.
  *, E-mail: hjmlovelx@hotmail.com
- Funds：
- DOI：10.24920/003495
  CLC：
- Received：09 August 2018，
  
  Accepted：2018-10-10，
  
  Published Online：04 December 2019，
  
  Published：04 December 2019
- Accepted：
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Liang Xi, Hu Jingnan, He Jianming. An Optimized Protocol of Azoxymethane-Dextran Sodium Sulfate Induced Colorectal Tumor Model in Mice[J]. Chinese medical sciences journal, 2019, 34(4): 281-288.
DOI：

Liang Xi, Hu Jingnan, He Jianming. An Optimized Protocol of Azoxymethane-Dextran Sodium Sulfate Induced Colorectal Tumor Model in Mice[J]. Chinese medical sciences journal, 2019, 34(4): 281-288. DOI： 10.24920/003495.

摘要

自氧化偶氮甲烷（AOM）-硫酸葡聚糖硫酸钠（DSS）诱导肿瘤用于研究炎症相关或散发性结直肠癌（CRC）以来

数种AOM、DSS处理方式被报导。本文优化了AOM-DSS诱导C57BL/6小鼠散发性结直肠癌模型的方法。利用100 μl消毒注射器按照需要剂量一次性腹腔注射适当浓度AOM

DSS处理7天并每天更换新鲜的DSS液体。我们共诱导超过100只小鼠

AOM处理后8至20周时

发现每只小鼠均被诱导发生结直肠肿瘤。肿瘤分布以结直肠远端居多

与人类结直肠癌相似。采用此方法建模的每只小鼠的成瘤数量较多、鼠间肿瘤数量差异较小。此AOM-DSS模型中的肿瘤病变涵盖了瘤前病变、腺瘤性病变、腺瘤以及腺癌。本改良的AOM-DSS结直肠癌建模方法具有简单、经济、成瘤率高等优点。

Abstract

Since azoxymethane (AOM)-dextran sodium sulfate (DSS) induced tumorigenesis was used to explore inflammation-associated carcinogenesis of sporadic colorectal cancer (CRC)

different administration modes of AOM or DSS have been reported. In this article we optimized the protocol of the AOM-DSS modeling using C57BL/6 mice for study on sporadic CRC by intraperitoneal injecting AOM solution at a proper concentration with a 100 μl sterile syringe once

feeding with DSS solution for 7 days in a roll and change DSS solution every day. More than 100 C57BL/6 mice had been treated with the optimized protocol

and all mice were demonstrated suffering from colorectal tumors when sacrificed in 8 to 20 weeks after AOM injection. These tumors mainly occurred in distal segment of colorectum with an increase in tumor density

which was similar to CRC in human beings. Tumor per mouse was high

and variation of tumor number per mouse was low. The histology of tumor developed through the defined stage ranged from precursor lesions

adenomatous lesions

adenomas to adenocarcinomas. The modified protocol of AOM-DSS model is easy

cheap

with high tumor formation rate of colorectal tumors.

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references

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