Association Between Homocysteine Level and Methylenetetrahydrofolate Reductase Gene Polymorphisms in Type 2 Diabetes Accompanied by Dyslipidemia

Yin Ying; Li Rui; Li Xiaoli; Wu Kunrong; Li Ling; Xu Yuedong; Liao Lin; Yang Rui; Li Yan

doi:10.24920/003532

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Association Between Homocysteine Level and Methylenetetrahydrofolate Reductase Gene Polymorphisms in Type 2 Diabetes Accompanied by Dyslipidemia

Chinese Medical Sciences Journal Vol. 35, Issue 1, Pages: 85-91(2020)

Original Article | Updated：2024-04-10

- Association Between Homocysteine Level and Methylenetetrahydrofolate Reductase Gene Polymorphisms in Type 2 Diabetes Accompanied by Dyslipidemia
- Affiliations：
  
  1School of Pharmaceutical Sciences, Shandong First Medical University & Shandong Academy of Medical Sciences, Tai’an, Shandong 271000, China
  2School of Pharmaceutical Sciences, Shandong University, Jinan 250012, China
  3Department of Pharmacy, Shandong Provincial Qianfoshan Hospital, Jinan 250014, China
  4Department of Endocrinology, Shandong Provincial Qianfoshan Hospital, Jinan 250014, China
- Author bio：
  
  * Tel: 86-531-89268352, E-mail: li_xyan@126.com
- Funds：
- DOI：10.24920/003532
  CLC：
- Received：12 May 2019，
  
  Published Online：31 March 2020，
  
  Published：07 March 2020
- Accepted：
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Yin Ying, Li Rui, Li Xiaoli, et al. Association Between Homocysteine Level and Methylenetetrahydrofolate Reductase Gene Polymorphisms in Type 2 Diabetes Accompanied by Dyslipidemia[J]. Chinese medical sciences journal, 2020, 35(1): 85-91.
DOI：

Yin Ying, Li Rui, Li Xiaoli, et al. Association Between Homocysteine Level and Methylenetetrahydrofolate Reductase Gene Polymorphisms in Type 2 Diabetes Accompanied by Dyslipidemia[J]. Chinese medical sciences journal, 2020, 35(1): 85-91. DOI： 10.24920/003532.

摘要

目的

探讨中国汉族血脂异常2型糖尿病（T2DM）患者血浆总同型半胱氨酸（tHcy）水平与亚甲基四氢叶酸还原酶（MTHFR）基因C677T和A1298C多态性的相关性。

方法

研究纳入179例中国汉族T2DM患者

采用sanger双脱氧链终止法检测MTHFR C677T和A1298C的基因多态性

采用酶循环法检测血浆tHcy。按照血脂水平将患者分为血脂正常组和异常组

采用卡方检验比较两组MTHFRC677T和A1298C多态性基因型频率和等位基因频率的差异

采用Student's t检验比较两组中携带不同基因型患者血浆tHcy水平的差异。

结果

研究纳入T2DM血脂异常患者82例

T2DM血脂正常患者94例。T2DM患者的MTHFR C677T多态性与tHcy水平之间存在显著相关性（

=2.27

=0.02）。携带MTHFR 677 TT基因型的血脂异常患者的血浆tHcy水平显著高于携带CT+CC基因型患者（13.62±6.97比10.95±3.62 μmol/L

=2.20

=0.03）;而对于血脂正常的患者

携带上述两个等位基因的患者的tHcy水平无显著差异（13.34±6.03比12.04±5.09 μmol/L

=1.08

=0.29）。

结论

MTHFR 677TT基因多态性可能与血脂异常的2型糖尿病患者的血浆tHcy水平升高有关。

Abstract

Objective

To investigate the association between total homocysteine (tHcy) level in plasma and methylenetetrahydrofolate reductase (

MTHFR

) C677T and A1298C genetic polymorphisms in a Chinese Han nationality population with type 2 diabetes mellitus (T2DM) accompanied by dyslipidemia.

Methods

This case-control study enrolled T2DM patients with dyslipidemia and without dyslipidemia respectively. Sanger dideoxy-mediated chain-termination method was used to detect the gene polymorphisms of

MTHFR

C677T and A1298C. Plasma tHcy and lipid levels were measured as well. The genotype frequency and allele frequency between the dyslipidemia and non-dyslipidemia groups were compared by using

Chi-square

test. Plasma tHcy level of T2DM patients who carried the different genotypes was compared by Student’s

test.

Results

Finally

82 T2DM patients with dyslipidemia and 94 ones without dyslipidemia were included in this study. There was a significant correlation between tHcy level and

MTHFR

C677T gene polymorphism in T2DM patients (

=2.27

=0.02). Moreover

the plasma tHcy level in the dyslipidemia patients who carried

MTHFR

677 TT genotype was significantly higher than that in those with CT+CC genotype (13.62±6.97

. 10.95±3.62 μmol/L

=2.20

=0.03); while for patients without dyslipidemia

comparison of the tHcy level between those who carried the above two alleles showed no significantly difference (13.34±6.03

. 12.04±5.09 μmol/L

=1.08

=0.29).

Conclusion

MTHFR

677TT genotype might associate with higher tHcy level in T2DM patients with dyslipidemia.

关键词

Keywords

references

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