Bacterial Spectrum and Antimicrobial Profile of Pediatric Blood Stream Infection at a Tertiary Care Hospital in Pakistan

Muhammad Anees; Noor Khan Sadiq; Jamal Tahir; Ul Haq Irshad; Jabbar Abdul; Ali Ihsan

doi:10.24920/003675

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Bacterial Spectrum and Antimicrobial Profile of Pediatric Blood Stream Infection at a Tertiary Care Hospital in Pakistan

Chinese Medical Sciences Journal Vol. 35, Issue 4, Pages: 315-322(2020)

Original Article | Updated：2024-04-10

- Bacterial Spectrum and Antimicrobial Profile of Pediatric Blood Stream Infection at a Tertiary Care Hospital in Pakistan
- Affiliations：
  
  1. 1Department of Medical Laboratory Technology, University of Haripur, Haripur, Khyber Pathtunkhwa (KPK) 22620, Pakistan
  2. 2Rehman Medical Institute (RMI), Peshawar, KPK 22620, Pakistan
  3. 3Department of Microbiology, University of Haripur, Haripur, KPK 22620, Pakistan
  4. 4College of Medical Technology, Bacha Khan Medical College, Mardan, KPK 23200, Pakistan
  5. 5Institute of Paramedical Sciences, Khyber Medical University, Peshawar, KPK 22620, Pakistan
- Author bio：
  
  * E-mail: ihsanmicro@gmail.com.
- Funds：
- DOI：10.24920/003675
  CLC：
- Received：29 October 2019，
  
  Accepted：2020-4-30，
  
  Published Online：31 December 2020，
  
  Published：31 December 2020
- Accepted：
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Muhammad Anees, Noor Khan Sadiq, Jamal Tahir, et al. Bacterial Spectrum and Antimicrobial Profile of Pediatric Blood Stream Infection at a Tertiary Care Hospital in Pakistan[J]. Chinese medical sciences journal, 2020, 35(4): 315-322.
DOI：

Muhammad Anees, Noor Khan Sadiq, Jamal Tahir, et al. Bacterial Spectrum and Antimicrobial Profile of Pediatric Blood Stream Infection at a Tertiary Care Hospital in Pakistan[J]. Chinese medical sciences journal, 2020, 35(4): 315-322. DOI： 10.24920/003675.

摘要

目的

血流感染（blood stream infection

BSI）是全球范围儿童发病和死亡的主要原因之一。本研究旨在确定BSI的流行程度

重点是鉴定BSI的病原体

并进一步评估致病细菌病原体的抗生素敏感性。

方法

于2018年1月至12月在巴基斯坦白沙瓦的三级医院进行横断面研究。血液样本采集于在BACTECTM瓶中

采用标准的微生物学方法对细菌菌株进行分离和鉴定。依据临床实验室标准化协会（Clinical Laboratory Standard Institute

CLSI）2014年指南

使用圆盘扩散法进行抗生素敏感性测试。

结果

共进行567次血液培养

其中BSI阳性111例（19.6％）。在阳性血培养中

男童占64％（71/111）

女童占36％（40/111）。微生物的致病优势菌群为革兰氏阴性菌79株（71.1％）

革兰氏阳性菌32株（28.9％）。分离株的常见细菌是伤寒沙门氏菌（

=35

31.5％）

大肠杆菌（

=19

17.1％）

金黄色葡萄球菌（

=18

16.2％）

肺炎克雷伯菌（

=12

10.8％）

及肠球菌（

6.3％）。有36.7％（29/79）的革兰氏阴性菌分离株是超广谱β-内酰胺酶（ESBL）产生者。61.1％（11/18）的金黄色葡萄球菌菌株对甲氧西林耐药。总体而言

72.9％的分离株具有多重耐药性。

结论

革兰氏阴性菌是儿童血流感染的主要病因

伤寒沙门氏菌是主要的致病菌

而大多数伤寒沙门氏菌分离株对环丙沙星耐药。

Abstract

Objectives

Blood stream infections (BSIs) are the main cause of morbidity and mortality in children worldwide. The present study was carried out to determine the prevalence of BSI with a focus on the identification of the causative agent of BSI

and to further evaluate the antibiotic susceptibility profile of the causing bacterial pathogens.

Methods

A cross-section study was carried out at the tertiary care hospital in Peshawar

Pakistan from January to December

2018. Blood samples were collected in BACTEC

bottles and standard microbiological protocols were applied for the isolation and identification of bacterial strains. The antibiotic susceptibility tests were performed using disc diffusion method as per the 2014 guideline of Clinical Laboratory Standard Institute (CLSI).

Results

Of 567 blood cultures in total

111(19.6%) were positive for BSI. Male children were 64 % (71/111) and female children were 36% (40/111). For the causative predominant group of microorganisms

Gram-negative bacteria were identified in 79 (71.1%) isolates

and Gram-positive bacteria in 32 (28.9%) isolates. The common bacteria of isolates were

S. typhi

(

=35

31.5%)

E. coli

(

=19

17.1%)

S. aureus

(

=18

16.2%)

K. pneumonia

(

=12

10.8%)

and

Enterococcus

species (

6.3%). The 36.7% (29/79) isolates of Gram-negative bacteria were ESBL producers

and 61.1% (11/18) of

S. aureus

isolates were methicillin resistant. Overall

72.9% isolates were multidrug resistant.

Conclusions

Gram-negative bacteria were the main cause of pediatric BSIs

where the predominant microorganism was

S. typhi

. Remarkably

majority of the

S. typhi

isolates were resistant to ciprofloxacin.

关键词

Keywords

references

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