Methylenetetrahydrofolate Reductase Gene Polymorphism C677T is Associated with Increased Risk of Coronary Heart Disease in Chinese Type 2 Diabetic Patients

Wu Kunrong; Zhang Shufang; Guan Ziwan; Li Xiaoli; Li Rui; Yin Ying; Li Yan

doi:10.24920/003792

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Methylenetetrahydrofolate Reductase Gene Polymorphism C677T is Associated with Increased Risk of Coronary Heart Disease in Chinese Type 2 Diabetic Patients

Chinese Medical Sciences Journal Vol. 36, Issue 2, Pages: 103-109(2021)

Original Article | Updated：2024-04-10

- Methylenetetrahydrofolate Reductase Gene Polymorphism C677T is Associated with Increased Risk of Coronary Heart Disease in Chinese Type 2 Diabetic Patients
- Affiliations：
  
  1Department of Clinical Pharmacy, The First Affiliated Hospital of Shandong First Medical University, Jinan 250014, China
  2School of Pharmaceutical Sciences, Shandong First Medical University (Shandong Academy of Medical Sciences) Tai’an 271000, Shandong, China
- Author bio：
  
  *Tel: 86-13791126823, E-mail: prof_liyan@126.com
- Funds：
- DOI：10.24920/003792
  CLC：
- Received：18 August 2020，
  
  Published Online：01 December 2020，
  
  Published：30 June 2021
- Accepted：
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Wu Kunrong, Zhang Shufang, Guan Ziwan, et al. Methylenetetrahydrofolate Reductase Gene Polymorphism C677T is Associated with Increased Risk of Coronary Heart Disease in Chinese Type 2 Diabetic Patients[J]. Chinese medical sciences journal, 2021, 36(2): 103-109.
DOI：

Wu Kunrong, Zhang Shufang, Guan Ziwan, et al. Methylenetetrahydrofolate Reductase Gene Polymorphism C677T is Associated with Increased Risk of Coronary Heart Disease in Chinese Type 2 Diabetic Patients[J]. Chinese medical sciences journal, 2021, 36(2): 103-109. DOI： 10.24920/003792.

摘要

目的

长期血糖控制不佳引起的慢性心血管疾病是2型糖尿病患者主要的死亡原因。研究表明：亚甲基四氢叶酸还原酶基因（methylenetetrahydrofolate reductase gene

MTHFR

）多态性可能会影响2型糖尿病患者冠心病的发生。本研究旨在评估

MTHFR

C677T和A1298C基因突变是否与2型糖尿病患者冠心病的发生风险相关。

方法

本研究一共纳入197例2型糖尿病受试者

其中95例受试者合并冠心病。采用双脱氧链终止法对

MTHFR

C677T和A1298C进行基因分型

并分析比较合并冠心病与不合并冠心病受试者等位基因频率的差异。

结果

合并冠心病2型糖尿病受试者的677T等位基因频率明显高于不合并冠心病的受试者（

=0.011）。单倍型频率在合并冠心病与不合并冠心病的2型糖尿病受试者之间无显著的差异。在同型半胱氨酸水平较低的糖尿病受试者中（≤15μmol/L）

携带677T等位基因的受试者发生冠心病风险更高（

=0.006）

而在同型半胱氨酸水平较高的糖尿病受试者中（

15μmol/L）

MTHFR

基因多态性对冠心病的发生风险无影响（

=0.491）。

结论

MTHFR

C677T基因多态性与糖尿病患者发生冠心病的风险相关

对于具有正常同型半胱氨酸水平的中国糖尿病人群

其可作为预测冠心病风险的有效标志物。

Abstract

Objective

Chronic cardiovascular diseases induced by long-term poor blood glucose control are the main cause of death in patients with type 2 diabetes mellitus (T2DM). Previous researches report that methylenetetrahydrofolate reductase gene (

MTHFR

) polymorphisms might influence the occurrence of coronary heart disease (CHD) in T2DM patients. The purpose of this study was to evaluate whether

MTHFR

C677T and A1298C mutations are associated with the risk of CHD in T2DM patients.

Methods

A total of 197 subjects with T2DM were studied

of which 95 patients with CHD. The genotypes of

MTHFR

C677T and A1298C were analyzed by using dideoxy chain-termination method

and compared between patients with CHD and those without CHD.

Results

We found that the frequency of the 677T allele was significantly higher in T2DM patients with CHD than those without CHD (

=0.011). However

there was no significant difference in any of the examined haplotypes between T2DM patients with and without CHD. Furthermore

the 677T allele was associated with a higher risk of CHD development in diabetic patients with lower homocysteine (Hcy) levels (≤15 μmol/L) (

=0.006)

while no effect of

MTHFR

gene polymorphism on the incidence of CHD was found in patients with higher Hcy levels (

15 μmol/L) (

=0.491).

Conclusion

The

MTHFR

C677T gene polymorphism is associated with the risk of CHD of diabetic patients and could be used as an effective marker for CHD in Chinese diabetic populations with normal Hcy levels.

关键词

Keywords

references

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Related Institution

School of Pharmaceutical Sciences, Shandong First Medical University & Shandong Academy of Medical Sciences, Tai’an

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Department of Pharmacy, Shandong Provincial Qianfoshan Hospital

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