Evaluation of the Gastric Microbiome in Patients with Chronic Superficial Gastritis and Intestinal Metaplasia

Liu Ying; Ma Yongjun; Huang Caiqun

doi:10.24920/003889

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Evaluation of the Gastric Microbiome in Patients with Chronic Superficial Gastritis and Intestinal Metaplasia

Chinese Medical Sciences Journal Vol. 37, Issue 1, Pages: 44-51(2022)

Original Article | Updated：2024-04-10

- Evaluation of the Gastric Microbiome in Patients with Chronic Superficial Gastritis and Intestinal Metaplasia
- Affiliations：
  
  1. 1Department of Clinical Laboratory, Jinhua Municipal Central Hospital, Jinhua, Zhejiang, 321000, China
  2. 2Department of Central Laboratory, Jinhua Municipal Central Hospital, Jinhua, Zhejiang, 321000, China
- Author bio：
  
  *E-mail: liuyingtj2008@sina.com.
- Funds：
- DOI：10.24920/003889
  CLC：
- Received：18 February 2021，
  
  Accepted：2021-9-18，
  
  Published Online：04 March 2022，
  
  Published：31 March 2022
- Accepted：
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Liu Ying, Ma Yongjun, Huang Caiqun. Evaluation of the Gastric Microbiome in Patients with Chronic Superficial Gastritis and Intestinal Metaplasia[J]. Chinese medical sciences journal, 2022, 37(1): 44-51.
DOI：

Liu Ying, Ma Yongjun, Huang Caiqun. Evaluation of the Gastric Microbiome in Patients with Chronic Superficial Gastritis and Intestinal Metaplasia[J]. Chinese medical sciences journal, 2022, 37(1): 44-51. DOI： 10.24920/003889.

摘要

目的

分析慢性浅表性胃炎患者和胃炎肠上皮化生患者胃内菌群的差异

探讨幽门螺杆菌感染对胃内菌群的影响。

方法

收集54例慢性浅表性胃炎和胃炎肠上皮化生患者的胃黏膜标本

根据是否感染幽门螺杆菌和病理检查

将其分为四组：幽门螺杆菌感染阴性慢性浅表性胃炎患者（n=24）、幽门螺杆菌感染阳性慢性浅表性胃炎患者（n=14）、幽门螺杆菌感染阴性胃炎肠上皮化生患者（n=11）和幽门螺杆菌感染阳性胃炎肠上皮化生患者（n=5）。采用16S rRNA基因测序的方法分析胃内菌群。

结果

幽门螺杆菌感染的患者胃内菌群的丰度和多样性明显低于未感染幽门螺杆菌的患者。幽门螺杆菌阴性组的患者具有相似的菌群组成和丰度。幽门螺杆菌阳性组的患者也有相似的菌群组成

但是细菌的相对丰度不同。幽门螺杆菌感染阳性胃炎肠上皮化生患者的奈瑟菌、链球菌、罗氏菌和韦荣球菌的相对丰度高于幽门螺杆菌感染阳性慢性浅表性胃炎患者

尤其是奈瑟菌（

=175.1

0.001）。

结论

幽门螺杆菌感染会明显降低胃内菌群的丰度和多样性。幽门螺杆菌感染阳性胃炎肠上皮化生患者的奈瑟菌、链球菌、罗氏菌和韦荣球菌的相对丰度高于幽门螺杆菌感染阳性慢性浅表性胃炎患者

尤其是奈瑟菌。

Abstract

Objective

To evaluate the gastric microbiome in patients with chronic superficial gastritis (CSG) and intestinal metaplasia (IM) and investigate the influence of

Helicobacter pylori

(

H. pylori

) on the gastric microbiome.

Methods

Gastric mucosa tissue samples were collected from 54 patients with CSG and IM

and the patients were classified into the following four groups based on the state of

H. pylori

infection and histology:

H. pylori

-negative CSG (

=24)

H. pylori

-positive CSG (

=14)

H. pylori

-negative IM (n=11)

and

H. pylori

-positive IM (

=5). The gastric microbiome was analyzed by 16S rRNA gene sequencing.

Results

H. pylori

strongly influenced the bacterial abundance and diversity regardless of CSG and IM. In

H. pylori

-positive subjects

the bacterial abundance and diversity were significantly lower than in

H. pylori

-negative subjects. The

H. pylori

-negative groups had similar bacterial composition and bacterial abundance. The

H. pylori

-positive groups also had similar bacterial composition but different bacterial relative abundance. The relative abundance of

Neisseria

Streptococcus

Rothia

and

Veillonella

were richer in the I-HP group than in G-HP group

especially

Neisseria

(

=175.1

0.001).

Conclusions

The gastric microbial abundance and diversity are lower in

H. pylori

- infected patients regardless of CSG and IM. Compared to

H. pylori

-positive CSG group and

H. pylori

-positive IM

the relative abundance of

Neisseria

Streptococcus

Rothia

and

Veillonella

is higher in

H. pylori

-positive patients with IM than in

H. pylori

-positive patients with CSG

especially

Neisseria.

关键词

Keywords

references

Marshall BJ , Warren JR . Unidentified curved bacilli in the stomach of patients with gastritis and peptic ulceration . Lancet 1984 ; 1 : 1311 - 5 . doi: 10.1016/s0140-6736(84)91816-6 https://dx.doi.org/10.1016/s0140-6736(84)91816-6 . DOI: 10.1016/s0140-6736(84)91816-6 http://doi.org/10.1016/s0140-6736(84)91816-6

Ianiro G , Molina-Infante J , Gasbarrini A . Gastric Microbiota . Helicobacter 2015 ; 20 ( Suppl 1 ): 68 - 71 . doi: 10.1111/hel.12260 https://dx.doi.org/10.1111/hel.12260 . DOI: 10.1111/hel.12260 http://doi.org/10.1111/hel.12260 http://doi.wiley.com/10.1111/hel.2015.20.issue-S1 http://doi.wiley.com/10.1111/hel.2015.20.issue-S1

Leja M , Grinberga-Derica I , Bilgilier C , et al . Review: Epidemiology of Helicobacter pylori infection . Helicobacter 2019 ; 24 ( Suppl 1 ): e12635 . doi: 10.1111/hel.12635 https://dx.doi.org/10.1111/hel.12635 . DOI: 10.1111/hel.12635 http://doi.org/10.1111/hel.12635

Polk DB , Peek RM Jr . Helicobacter pylori: Gastric cancer and beyond . Nat Rev Cancer 2010 ; 10 ( 6 ): 403 - 14 . doi: 10.1038/nrc2857 https://dx.doi.org/10.1038/nrc2857 . DOI: 10.1038/nrc2857 http://doi.org/10.1038/nrc2857 https://doi.org/10.1038/nrc2857 https://doi.org/10.1038/nrc2857

Dias-Jácome E , Libânio D , Borges-Canha M , et al . Gastric microbiota and carcinogenesis: the role of non-Helicobacter pylori bacteria - A systematic review . Rev Esp Enferm Dig 2016 ; 108 ( 9 ): 530 - 40 . doi: 10.17235/reed.2016.4261/2016 https://dx.doi.org/10.17235/reed.2016.4261/2016 . DOI: 10.17235/reed.2016.4261/2016 http://doi.org/10.17235/reed.2016.4261/2016

Uemura N , Okamoto S , Yamamoto S , et al . Helicobacter pylori infection and the development of gastric cancer . N Engl J Med 2001 ; 345 ( 11 ): 784 - 9 . doi: 10.1056/NEJMoa001999 https://dx.doi.org/10.1056/NEJMoa001999 . DOI: 10.1056/NEJMoa001999 http://doi.org/10.1056/NEJMoa001999 http://www.nejm.org/doi/abs/10.1056/NEJMoa001999 http://www.nejm.org/doi/abs/10.1056/NEJMoa001999

Ma JL , Zhang L , Brown LM , et al . Fifteen-year effects of Helicobacter pylori, garlic, and vitamin treatments on gastric cancer incidence and mortality . J Natl Cancer Inst 2012 ; 104 : 488 - 92 . doi: 10.1093/jnci/djs003 https://dx.doi.org/10.1093/jnci/djs003 . DOI: 10.1093/jnci/djs003 http://doi.org/10.1093/jnci/djs003 https://academic.oup.com/jnci/article-lookup/doi/10.1093/jnci/djs003 https://academic.oup.com/jnci/article-lookup/doi/10.1093/jnci/djs003

Li WQ , Ma JL , Zhang L , et al . Effects of Helicobacter pylori treatment on gastric cancer incidence and mortality in subgroups . J Natl Cancer Inst 2014 ; 106 ( 7 ):dju116. doi: 10.1093/jnci/dju116 https://dx.doi.org/10.1093/jnci/dju116 . DOI: 10.1093/jnci/dju116 http://doi.org/10.1093/jnci/dju116

Wong BC , Zhang L , Ma JL , et al . Effects of selective COX-2 inhibitor and Helicobacter pylori eradication on precancerous gastric lesions . Gut 2012 ; 61 ( 6 ): 812 - 8 . doi: 10.1136/gutjnl-2011-300154 https://dx.doi.org/10.1136/gutjnl-2011-300154 . DOI: 10.1136/gutjnl-2011-300154 http://doi.org/10.1136/gutjnl-2011-300154 https://gut.bmj.com/lookup/doi/10.1136/gutjnl-2011-300154 https://gut.bmj.com/lookup/doi/10.1136/gutjnl-2011-300154

International Agency for Research on Cancer . Helicobacter pylori eradication as a strategy for preventing gastric cancer . IARC working group reports Volume 8. Available from https://www.gastro-health-now.org/wp/wp-content/uploads/2014/09/WHO-IARC-Report-2014.pdf. accessed: January 1. https://www.gastro-health-now.org/wp/wp-content/uploads/2014/09/WHO-IARC-Report-2014.pdf https://www.gastro-health-now.org/wp/wp-content/uploads/2014/09/WHO-IARC-Report-2014.pdf

Pohl D , Keller PM , Bordier V , et al . Review of current diagnostic methods and advances in Helicobacter pylori diagnostics in the era of next generation sequencing . World J Gastroenterol 2019 ; 25 ( 32 ): 4629 - 4660 . doi: 10.3748/wjg.v25.i32.4629 https://dx.doi.org/10.3748/wjg.v25.i32.4629 . DOI: 10.3748/wjg.v25.i32.4629 http://doi.org/10.3748/wjg.v25.i32.4629 https://www.wjgnet.com/1007-9327/full/v25/i32/4629.htm https://www.wjgnet.com/1007-9327/full/v25/i32/4629.htm

Zhao Y , Gao X , Guo J , et al . Helicobacter pylori infection alters gastric and tongue coating microbial communities . Helicobacter. 2019 ; 24 ( 2 ): e12567 . doi: 10.1111/hel.12567 https://dx.doi.org/10.1111/hel.12567 . DOI: 10.1111/hel.12567 http://doi.org/10.1111/hel.12567 http://doi.wiley.com/10.1111/hel.2019.24.issue-2 http://doi.wiley.com/10.1111/hel.2019.24.issue-2

Gantuya B , El Serag HB , Saruuljavkhlan B , et al . Advantage of 16S rRNA amplicon sequencing in Helicobacter pylori diagnosis . Helicobacter 2021 ; 26 ( 3 ): e12790 . doi: 10.1111/hel.12790 https://dx.doi.org/10.1111/hel.12790 . DOI: 10.1111/hel.12790 http://doi.org/10.1111/hel.12790

Park CH , Lee AR , Lee YR , et al . Evaluation of gastric microbiome and metagenomic function in patients with intestinal metaplasia using 16S rRNA gene sequencing . Helicobacter 2019 ; 24 ( 1 ): e12547 . doi: 10.1111/hel.12547 https://dx.doi.org/10.1111/hel.12547 . DOI: 10.1111/hel.12547 http://doi.org/10.1111/hel.12547 http://doi.wiley.com/10.1111/hel.2019.24.issue-1 http://doi.wiley.com/10.1111/hel.2019.24.issue-1

Schulz C , Schütte K , Koch N , et al . The active bacterial assemblages of the upper GI tract in individuals with and without Helicobacter infection . Gut 2018 ; 67 ( 2 ): 216 - 25 . doi: 10.1136/gutjnl-2016-312904 https://dx.doi.org/10.1136/gutjnl-2016-312904 . DOI: 10.1136/gutjnl-2016-312904 http://doi.org/10.1136/gutjnl-2016-312904 https://gut.bmj.com/lookup/doi/10.1136/gutjnl-2016-312904 https://gut.bmj.com/lookup/doi/10.1136/gutjnl-2016-312904

Parsons BN , Ijaz UZ , D’Amore R , et al . Comparison of the human gastric microbiota in hypochlorhydric states arising as a result of Helicobacter pylori-induced atrophic gastritis, autoimmune atrophic gastritis and proton pump inhibitor use . PLoS Pathog 2017 ; 13 ( 11 ): e1006653 . doi: 10.1371/journal https://dx.doi.org/10.1371/journal . DOI: 10.1371/journal http://doi.org/10.1371/journal https://dx.plos.org/10.1371/journal.ppat.1006653 https://dx.plos.org/10.1371/journal.ppat.1006653

Klymiuk I , Bilgilier C , Stadlmann A , et al . The Human Gastric Microbiome Is Predicated upon Infection with Helicobacter pylori . Front Microbiol 2017 ; 8 : 2508 . doi: 10.3389/fmicb.2017.02508 https://dx.doi.org/10.3389/fmicb.2017.02508 . DOI: 10.3389/fmicb.2017.02508 http://doi.org/10.3389/fmicb.2017.02508 http://journal.frontiersin.org/article/10.3389/fmicb.2017.02508/full http://journal.frontiersin.org/article/10.3389/fmicb.2017.02508/full

Ahn J , Sinha R , Pei Z , et al . Human gut microbiome and risk for colorectal cancer . J Natl Cancer Inst 2013 ; 105 ( 24 ): 1907 - 11 . doi: 10.1093/jnci/djt300 https://dx.doi.org/10.1093/jnci/djt300 . DOI: 10.1093/jnci/djt300 http://doi.org/10.1093/jnci/djt300 https://academic.oup.com/jnci/article-lookup/doi/10.1093/jnci/djt300 https://academic.oup.com/jnci/article-lookup/doi/10.1093/jnci/djt300

Coker OO , Dai Z , Nie Y , et al . Mucosal microbiome dysbiosis in gastric carcinogenesis . Gut 2018 ; 67 ( 6 ): 1024 - 32 . doi: 10.1136/gutjnl-2017-314281 https://dx.doi.org/10.1136/gutjnl-2017-314281 . DOI: 10.1136/gutjnl-2017-314281 http://doi.org/10.1136/gutjnl-2017-314281 https://gut.bmj.com/lookup/doi/10.1136/gutjnl-2017-314281 https://gut.bmj.com/lookup/doi/10.1136/gutjnl-2017-314281

Ferreira RM , Pereira-Marques J , Pinto-Ribeiro I , et al . Gastric microbial community profiling reveals a dysbiotic cancer-associated microbiota . Gut 2018 ; 67 ( 2 ): 226 - 36 . doi: 10.1136/gutjnl-2017-314205 https://dx.doi.org/10.1136/gutjnl-2017-314205 . DOI: 10.1136/gutjnl-2017-314205 http://doi.org/10.1136/gutjnl-2017-314205

Palrasu M , Zaika E , El-Rifai W , et al . Role of Bacterial and Viral Pathogens in Gastric Carcinogenesis . Cancers (Basel) 2021 ; 13 ( 8 ): 1878 . doi: 10.3390/cancers13081878 https://dx.doi.org/10.3390/cancers13081878 . DOI: 10.3390/cancers13081878 http://doi.org/10.3390/cancers13081878 https://www.mdpi.com/2072-6694/13/8/1878 https://www.mdpi.com/2072-6694/13/8/1878

Song H , Ekheden IG , Zheng Z , et al . Incidence of gastric cancer among patients with gastric precancerous lesions: observational cohort study in a low risk Western population . BMJ 2015 ; 351 : h3867 . doi: 10.1136/bmj.h3867 https://dx.doi.org/10.1136/bmj.h3867 . DOI: 10.1136/bmj.h3867 http://doi.org/10.1136/bmj.h3867

Amieva M , Peek RM Jr . Athobiology of helicobacter pylori-induced gastric cancer . Gastroenterology 2016 ; 150 ( 1 ): 64 - 78 . doi: 10.1053/j.gastro.2015.09.004 https://dx.doi.org/10.1053/j.gastro.2015.09.004 . DOI: 10.1053/j.gastro.2015.09.004 http://doi.org/10.1053/j.gastro.2015.09.004 https://linkinghub.elsevier.com/retrieve/pii/S0016508515013128 https://linkinghub.elsevier.com/retrieve/pii/S0016508515013128

Li F , Zhu H , Tao K , et al . Mucosal microbial microenvironment in early gastric neoplasia and non-neoplastic gastric disease . J Gastroenterol Hepatol 2021 ; 36 ( 11 ): 3092 - 101 . doi: 10.1111/jgh.15565 https://dx.doi.org/10.1111/jgh.15565 . DOI: 10.1111/jgh.15565 http://doi.org/10.1111/jgh.15565 https://onlinelibrary.wiley.com/toc/14401746/36/11 https://onlinelibrary.wiley.com/toc/14401746/36/11

Guo Y , Zhang Y , Gerhard M , et al . Effect of Helicobacter pylori on gastrointestinal microbiota: a population-based study in Linqu, a high-risk area of gastric cancer . Gut 2020 ; 69 ( 9 ): 1598 - 607 . doi: 10.1136/gutjnl-2019-319696 https://dx.doi.org/10.1136/gutjnl-2019-319696 . DOI: 10.1136/gutjnl-2019-319696 http://doi.org/10.1136/gutjnl-2019-319696 https://gut.bmj.com/lookup/doi/10.1136/gutjnl-2019-319696 https://gut.bmj.com/lookup/doi/10.1136/gutjnl-2019-319696

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