Recombinant Expression and Purification of Mouse Nectin-like 4 Glycoprotein in 293ET Cell Line

Li Dongdong; An Tai; Liu Xiao; Yin Bin; Peng Xiaozhong; Shu Pengcheng

doi:10.24920/11805

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Recombinant Expression and Purification of Mouse Nectin-like 4 Glycoprotein in 293ET Cell Line

Chinese Medicine Science Journal Vol. 33, Issue 1, Pages: 1-8(2018)

ORIGINAL ARTICLE | Updated：2024-04-10

- Recombinant Expression and Purification of Mouse Nectin-like 4 Glycoprotein in 293ET Cell Line
- Affiliations：
  
  中国医学科学院基础医学研究所,北京协和医学院基础学院,国家医学分子生物重点实验室,北京 100005
- Author bio：
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- DOI：10.24920/11805
  CLC：
- Received：23 October 2017，
  
  Published Online：17 March 2017，
  
  Published：01 April 2018
- Accepted：
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Li Dongdong, An Tai, Liu Xiao, et al. Recombinant Expression and Purification of Mouse Nectin-like 4 Glycoprotein in 293ET Cell Line[J]. Chinese medicine science journal, 2018, 33(1): 1-8.
DOI：

Li Dongdong, An Tai, Liu Xiao, et al. Recombinant Expression and Purification of Mouse Nectin-like 4 Glycoprotein in 293ET Cell Line[J]. Chinese medicine science journal, 2018, 33(1): 1-8. DOI： 10.24920/11805.

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Samanta D , Almo SC . Nectin family of cell-adhesion molecules: structural and molecular aspects of function and specificity . Cell Mol Life Sci , 2015 ; 72 ( 4 ): 645 - 58 . doi: 10.1007/s00018-014-1763-4 https://doi.org/10.1007/s00018-014-1763-4 . DOI: 10.1007/s00018-014-1763-4 http://doi.org/10.1007/s00018-014-1763-4 http://link.springer.com/10.1007/s00018-014-1763-4 http://link.springer.com/10.1007/s00018-014-1763-4

Mandai K , Rikitake Y , Mori M , et al . Nectins and nectin-like molecules in development and disease . Curr Top Dev Biol , 2015 ; 112 : 197 - 231 . doi: 10.1016/bs.ctdb.2014.11.019 https://doi.org/10.1016/bs.ctdb.2014.11.019 . DOI: 10.1016/bs.ctdb.2014.11.019 http://doi.org/10.1016/bs.ctdb.2014.11.019 http://linkinghub.elsevier.com/retrieve/pii/S0070215314000209 http://linkinghub.elsevier.com/retrieve/pii/S0070215314000209

Galibert L , Diemer GS , Liu Z , et al . Nectin-like protein 2 defines a subset of T-cell zone dendritic cells and is a ligand for class-I-restricted T-cell-associated molecule . J Biol Chem , 2005 ; 280 ( 23 ): 21955 - 64 . doi: 10.1074/jbc.M502095200 https://doi.org/10.1074/jbc.M502095200 . DOI: 10.1074/jbc.M502095200 http://doi.org/10.1074/jbc.M502095200 http://www.jbc.org/lookup/doi/10.1074/jbc.M502095200 http://www.jbc.org/lookup/doi/10.1074/jbc.M502095200

Kakunaga S , Ikeda W , Itoh S , et al . Nectin-like molecule-1/TSLL1/SynCAM3: a neural tissue-specific immunoglobulin-like cell-cell adhesion molecule localizing at non-junctional contact sites of presynaptic nerve terminals, axons and glia cell processes . J Cell Sci , 2005 ; 118 ( 6 ): 1267 - 77 . doi: 10.1242/jcs.01656 https://doi.org/10.1242/jcs.01656 . DOI: 10.1242/jcs.01656 http://doi.org/10.1242/jcs.01656 http://jcs.biologists.org/cgi/doi/10.1242/jcs.01656 http://jcs.biologists.org/cgi/doi/10.1242/jcs.01656

Mizutani K , Takai Y . Nectin spot: a novel type of nectin-mediated cell adhesion apparatus . Biochem J , 2016 ; 473 ( 18 ): 2691 - 715 . doi: 10.1042/BCJ20160235 https://doi.org/10.1042/BCJ20160235 . DOI: 10.1042/BCJ20160235 http://doi.org/10.1042/BCJ20160235 http://biochemj.org/cgi/doi/10.1042/BCJ20160235 http://biochemj.org/cgi/doi/10.1042/BCJ20160235

Catros V , Dessarthe B . Nectins and nectin-like recaeptors DNAM-1 and CRTAM: new ways for tumor escape . Med Sci , 2014 ; 30 ( 5 ): 537 - 43 . doi: 10.1051/medsci/20143005017 https://doi.org/10.1051/medsci/20143005017 DOI: 10.1051/medsci/20143005017 http://doi.org/10.1051/medsci/20143005017 http://www.ncbi.nlm.nih.gov/pubmed/24939541 http://www.ncbi.nlm.nih.gov/pubmed/24939541

Zhou Y , Du G , Hu X , et al . Nectin-like molecule 1 is a protein 4.1N associated protein and recruits protein 4.1N from cytoplasm to the plasma membrane . Biochim Biophys Acta , 2005 ; 1669 ( 2 ): 142 - 54 . doi: 10.1016/j.bbamem.2005.01.013 https://doi.org/10.1016/j.bbamem.2005.01.013 . DOI: 10.1016/j.bbamem.2005.01.013 http://doi.org/10.1016/j.bbamem.2005.01.013 http://linkinghub.elsevier.com/retrieve/pii/S0005273605000283 http://linkinghub.elsevier.com/retrieve/pii/S0005273605000283

Maurel P , Einheber S , Galinska J , et al . Nectin-like proteins mediate axon Schwann cell interactions along the internode and are essential for myelination . J Cell Biol , 2007 ; 178 ( 5 ): 861 - 74 . doi: 10.1083/jcb.200705132 https://doi.org/10.1083/jcb.200705132 . DOI: 10.1083/jcb.200705132 http://doi.org/10.1083/jcb.200705132 http://www.jcb.org/lookup/doi/10.1083/jcb.200705132 http://www.jcb.org/lookup/doi/10.1083/jcb.200705132

Spiegel I , Adamsky K , Eshed Y , et al . A central role for Necl4 (SynCAM4) in Schwann cell-axon interaction and myelination . Nat Neurosci , 2007 ; 10 ( 7 ): 861 - 9 . doi: 10.1038/nn1915 https://doi.org/10.1038/nn1915 . DOI: 10.1038/nn1915 http://doi.org/10.1038/nn1915 http://www.nature.com/articles/nn1915 http://www.nature.com/articles/nn1915

Park J , Liu B , Chen T , et al . Disruption of Nectin-like 1 cell adhesion molecule leads to delayed axonal myelination in the CNS . J Neurosci , 2008 ; 28 ( 47 ): 12815 - 9 . doi: 10.1523/JNEUROSCI.2665-08.2008 https://doi.org/10.1523/JNEUROSCI.2665-08.2008 . DOI: 10.1523/JNEUROSCI.2665-08.2008 http://doi.org/10.1523/JNEUROSCI.2665-08.2008 http://www.jneurosci.org/cgi/doi/10.1523/JNEUROSCI.2665-08.2008 http://www.jneurosci.org/cgi/doi/10.1523/JNEUROSCI.2665-08.2008

Heffernan C , Jain MR , Liu T , et al . Nectin-like 4 complexes with choline transporter-like protein-1 and regulates Schwann cell choline homeostasis and lipid biogenesis in vitro . J Biol Chem , 2017 ; 292 ( 11 ): 4484 - 98 . doi: 10.1074/jbc.M116.747816 https://doi.org/10.1074/jbc.M116.747816 . DOI: 10.1074/jbc.M116.747816 http://doi.org/10.1074/jbc.M116.747816 http://www.jbc.org/lookup/doi/10.1074/jbc.M116.747816 http://www.jbc.org/lookup/doi/10.1074/jbc.M116.747816

Zhu Y , Li H , Li K , et al . Necl-4/SynCAM-4 is expressed in myelinating oligodendrocytes but not required for axonal myelination . PLoS One , 2013 ; 8 ( 5 ): e64264 . doi: 10.1371/journal.pone.0064264 https://doi.org/10.1371/journal.pone.0064264 .

Golan N , Kartvelishvily E , Spiegel I , et al . Genetic deletion of Cadm4 results in myelin abnormalities resembling Charcot-Marie-Tooth neuropathy . J Neurosci , 2013 ; 33 ( 27 ): 10950 - 61 . doi: 10.1523/JNEUROSCI.0571-13.2013 https://doi.org/10.1523/JNEUROSCI.0571-13.2013 . DOI: 10.1523/JNEUROSCI.0571-13.2013 http://doi.org/10.1523/JNEUROSCI.0571-13.2013 http://www.jneurosci.org/cgi/doi/10.1523/JNEUROSCI.0571-13.2013 http://www.jneurosci.org/cgi/doi/10.1523/JNEUROSCI.0571-13.2013

Yang S , Weng H , Chen L , et al . Lack of protein 4.1G causes altered expression and localization of the cell adhesion molecule nectin-like 4 in testis and can cause male infertility . Mol Cell Biol , 2011 ; 31 ( 11 ): 2276 - 86 . doi: 10.1128/MCB.01105-10 https://doi.org/10.1128/MCB.01105-10 . DOI: 10.1128/MCB.01105-10 http://doi.org/10.1128/MCB.01105-10 http://mcb.asm.org/cgi/doi/10.1128/MCB.01105-10 http://mcb.asm.org/cgi/doi/10.1128/MCB.01105-10

Raveh S , Gavert N , Spiegel I , et al . The cell adhesion nectin-like molecules (Necl) 1 and 4 suppress the growth and tumorigenic ability of colon cancer cells . J Cell Biochem , 2009 ; 108 ( 1 ): 326 - 36 . doi: 10.1002/jcb.22258 https://doi.org/10.1002/jcb.22258 . DOI: 10.1002/jcb.v108:1 http://doi.org/10.1002/jcb.v108:1 http://doi.wiley.com/10.1002/jcb.v108%3A1 http://doi.wiley.com/10.1002/jcb.v108%3A1

Dong X , Xu F , Gong Y , et al . Crystal structure of the V domain of human Nectin-like molecule-1/Syncam3/Tsll1/Igsf4b, a neural tissue-specific immunoglobulin-like cell-cell adhesion molecule . J Biol Chem , 2006 ; 281 ( 15 ): 10610 - 7 . doi: 10.1074/jbc.M513459200 https://doi.org/10.1074/jbc.M513459200 . DOI: 10.1074/jbc.M513459200 http://doi.org/10.1074/jbc.M513459200 http://www.jbc.org/lookup/doi/10.1074/jbc.M513459200 http://www.jbc.org/lookup/doi/10.1074/jbc.M513459200

Fogel AI , Li Y , Giza J , et al . N-glycosylation at the SynCAM (synaptic cell adhesion molecule) immunoglobulin interface modulates synaptic adhesion . J Biol Chem , 2010 ; 285 ( 45 ): 34864 - 74 . doi: 10.1074/jbc.M110.120865 https://doi.org/10.1074/jbc.M110.120865 . DOI: 10.1074/jbc.M110.120865 http://doi.org/10.1074/jbc.M110.120865 http://www.jbc.org/lookup/doi/10.1074/jbc.M110.120865 http://www.jbc.org/lookup/doi/10.1074/jbc.M110.120865

Liu J , Qian X , Chen Z , et al . Crystal structure of cell adhesion molecule nectin-2/CD112 and its binding to immune receptor DNAM-1/CD226 . J Immunol , 2012 ; 188 ( 11 ): 5511 - 20 . doi: 10.4049/jimmunol.1200324 https://doi.org/10.4049/jimmunol.1200324 . DOI: 10.4049/jimmunol.1200324 http://doi.org/10.4049/jimmunol.1200324 http://www.jimmunol.org/cgi/doi/10.4049/jimmunol.1200324 http://www.jimmunol.org/cgi/doi/10.4049/jimmunol.1200324

Gao J , Chen T , Hu G , et al . Nectin-like molecule 1 is a glycoprotein with a single N-glycosylation site at N290KS which influences its adhesion activity . Biochim Biophys Acta , 2008 ; 1778 ( 6 ): 1429 - 35 . doi: 10.1016/j.bbamem.2008.03.013 https://doi.org/10.1016/j.bbamem.2008.03.013 . DOI: 10.1016/j.bbamem.2008.03.013 http://doi.org/10.1016/j.bbamem.2008.03.013 http://linkinghub.elsevier.com/retrieve/pii/S0005273608001089 http://linkinghub.elsevier.com/retrieve/pii/S0005273608001089

Chang VT , Crispin M , Aricescu AR , et al . Glycoprotein structural genomics: solving the glycosylation problem . Structure , 2007 ; 15 ( 3 ): 267 - 73 . doi: 10.1016/j.str.2007.01.011 https://doi.org/10.1016/j.str.2007.01.011 . DOI: 10.1016/j.str.2007.01.011 http://doi.org/10.1016/j.str.2007.01.011 http://linkinghub.elsevier.com/retrieve/pii/S0969212607000664 http://linkinghub.elsevier.com/retrieve/pii/S0969212607000664

Elbein AD , Tropea JE , Mitchell M , et al . Kifunensine, a potent inhibitor of the glycoprotein processing mannosidase I . J Biol Chem , 1990 ; 265 ( 26 ): 15599 - 605 . DOI: 10.1016/0014-5793(90)81275-S http://doi.org/10.1016/0014-5793(90)81275-S http://www.ncbi.nlm.nih.gov/pubmed/2144287 http://www.ncbi.nlm.nih.gov/pubmed/2144287

Flanagan JG , Leder P . The kit ligand: a cell surface molecule altered in steel mutant fibroblasts . Cell , 1990 ; 63 ( 1 ): 185 - 94 . doi: 10.1016/0092-8674(90)90299-T https://doi.org/10.1016/0092-8674(90)90299-T . DOI: 10.1016/0092-8674(90)90299-T http://doi.org/10.1016/0092-8674(90)90299-T http://linkinghub.elsevier.com/retrieve/pii/009286749090299T http://linkinghub.elsevier.com/retrieve/pii/009286749090299T

Flanagan JG , Cheng HJ . Alkaline phosphatase fusion proteins for molecular characterization and cloning of receptors and their ligands . Methods Enzymol , 2000 ; 327 : 198 - 210 . doi: 10.1016/S0076-6879(00)27277-7 https://doi.org/10.1016/S0076-6879(00)27277-7 . DOI: 10.1016/S0076-6879(00)27277-7 http://doi.org/10.1016/S0076-6879(00)27277-7 http://linkinghub.elsevier.com/retrieve/pii/S0076687900272777 http://linkinghub.elsevier.com/retrieve/pii/S0076687900272777

Flanagan JG , Cheng HJ , Feldheim DA , et al . Alkaline phosphatase fusions of ligands or receptors as in situ probes for staining of cells, tissues, and embryos . Methods Enzymol , 2000 ; 327 : 19 - 35 . doi: 10.1016/S0076-6879(00)27264-9 https://doi.org/10.1016/S0076-6879(00)27264-9 . DOI: 10.1016/S0076-6879(00)27264-9 http://doi.org/10.1016/S0076-6879(00)27264-9 http://linkinghub.elsevier.com/retrieve/pii/S0076687900272649 http://linkinghub.elsevier.com/retrieve/pii/S0076687900272649

Zhang Y , Chao T , Li R , et al . MicroRNA-128 inhibits glioma cells proliferation by targeting transcription factor E2F3a . J Mol Med (Berl) , 2009 ; 87 ( 1 ): 43 - 51 . doi: 10.1007/s00109-008-0403-6 https://doi.org/10.1007/s00109-008-0403-6 . DOI: 10.1007/s00109-008-0403-6 http://doi.org/10.1007/s00109-008-0403-6 http://link.springer.com/10.1007/s00109-008-0403-6 http://link.springer.com/10.1007/s00109-008-0403-6

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