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Volume ， Issue 2 ， 1994
- Abstract：Genetically-engineered CHO cell lines, rβ- 13 and CLF-8B2, were cultivated with the MC- 1 microcarrier culture system. The cell density could be enhanced by increasing the concentration of microcarrier. At a microcarrier concentration of 10 mg/ml. the cell density could reach 4 to 5 × 106 cells/ml. It was shown that these cell lines would spontaneously release from the microcarrier to attach to and proliferate on fresh microcarriers. We were thus able to scale up cultivation using a simple method. i. e. by adding fresh microcarriers and medium directly into the culture system to about 2, 4 or 8 times the original volume. Using a perfusion culture system. we have successfully cultivated CLF-8B2 cells in a 2 L bioreactor for several weeks at medium perfusion rates of 0. 5 to 3working volumes. Prourokinase was stably secreted.4|26|0Updated：2024-04-10
- Abstract：Boanmycin （bleomycin A6 . BM） . an antitumor antibiotic, was conjugated to monoclonal antibodies including R19, H 111 and CCT2. The immunoconjugates exhibited selective cytotoxicity to related target cells including cecum cancer Hce-8693 cells, liver cancer BEL-7402 cells and leukemia CEM cells. They were highly effective against related human tumor xenografts in nude mice, and the inhibition rates by the conjugates were much higher than those by free BM. The inhibition rate by R19-BM conjugate against human cecum cancer xenografts reached 90%. BY immunoelectron microscopy, CCT2-BM conjugate showed specific binding and internalization in leukemia CEM cells. The results indicate that boanmycin-monoclonal antibody immunoconjugates are highly active both in vitro and in vivo.4|18|1Updated：2024-04-10
- Abstract：ＳｕｂｓｔａｎｃｅＰｉｎｔｈｅＭｏｔｏｎｅｕｒｏｎＡｒｅａｓｏｆｔｈｅＶｅｎｔｒａｌＳｐｉｎａｌＣｏｒｄｉｎｔｈｅＣａｔ：ＩｍｍｕｎｏｅｌｅｃｔｒｏｎＭｉｃｒｏｓｃｏｐｉｃＳｔｕｄｙ￥ＨｕａｎｇＹａｏｄｅ（黄跃德）；ＨｏｎｇＸｉｕｈｕａ（洪秀华）；ｅ...4|4|0Updated：2024-04-10
- Abstract：It had been found that Beijing ducks（BD） have a high level of HDL（70%）, high LCAT but very low CETP activity and will not develop atherosclerosis on an atherogenic diet, suggesting that cholesterol ester is mainly carried by HDL and metabolized through an HDL receptor pathway in the liver. However, evidence of this receptor′s existence in the liver is not yet complete. In this paper. the HDL receptor in BD liver has been studied. Our experiments showed : 1 ) ApoE-free 125 I-HDL could bind specifically to duck hepatic cell membrane with high affinity （Kd=9.6μg/ml） and was saturable （Bmax=8.9μg/mg cell membrane protein）at room temperature 2)Competitive inhibition studies with unlabelled duck, human, rat and chick HDL and duck apo AI and its liposomes formed with PC or DMPC could inhibit the binding of 125I-HDL to duck hepatic cell membranes, but LDL,apo E and their liposomes with PC or DMPC could not with the exception of duck LDL. 3) The receptor could recognize apo AI but not apo B or E. 4 ) Both phosphorase A2 and pronase could inhibit the binding activity. The above results give strong evidence for the existence of a specific HDL receptor pathway in the duck liver, supporting our hypothesis that CE in Beijing ducks is metabolized directly through the hepatic HDL receptor instead of being transfered back to VLDL and LDL, then through the LDL receptor pathway. This unique way of metabolizing CE may be behind the Beijing duck’s antiatherogenicity.4|6|0Updated：2024-04-10
- Abstract：ＳｕｂｓｔａｎｃｅＰ－ａｎｄＣｈｏｌｉｎｅＡｃｅｔｙｌｔｒａｎｓｆｅｒａｓｅ－ｌｉｋｅＩｍｍｕｎｏｒｅａｃｔｉｖｉｔｙｉｎｔｈｅＭｏｔｏｎｅｕｒｏｎＡｒｅａｓｏｆｔｈｅＣａｔＶｅｎｔｒａｌＳｐｉｎａｌＣｏｒｄ￥ＨｕａｎｇＹａｏｄｅ（黄跃德）；Ｈｏｎｇ...4|3|0Updated：2024-04-10
- Abstract：Toad red blood cells were used to measure regional bone blood flow in the canine mandibular ramus. The blood cells were labelled with sodium pertechnetate and fixed in 1 0%formalin; they were 22 × 15 μm in size and had a specific gravity close to that of dog red blood cells. These cells had no discernible effect on systemic hemodynamics after injection, did not agglutinate, were well mixed and evenly distributed throughout the body, and were completely extracted in one circulation through the mandible. The mandibular ramus was divided into six regions. and the blood flow rates in each were found to be similar to those reported in previous studies with radiolabelled carbonized, microspheres. Furthermore, the blood flow distribution pattern of the mandibular ramus determined in this study was identical to that of our previous study using the bone-seeking radionuclide method. We suggest that radiolabelled toad red blood cells are an ideal marker for measuring regional blood flow in the canine mandible.4|10|0Updated：2024-04-10
- Abstract：ＧａｓｔｒｏｓｃｏｐｉｃＯｂｓｅｒｖａｔｉｏｎａｎｄＡｎａｌｙｓｉｓｏｆ８９ＣａｓｅｓｏｆＰｏｒｔａｌＨｙｐｅｒｔｅｎｓｉｖｅＧａｓｔｒｏｄｕｏｄｅｎａｌＭｕｃｏｓａｌＬｅｓｉｏｎｓ￥ＣｈｏｕＺｅｎｇｆｅｎ（周曾芬）(ＤｅｐａｒｔｍｅｎｔｏｆＧａｓｔ...4|3|0Updated：2024-04-10
- Abstract：A monoclonal antibody raised against bone morphogenetic protein （BMP-McAb） has been used to demonstrate the presence of bone morphogenetic protein（BMP） in experimental fracture healing. Rabbit mandibles were fractured using standardized methods and left to heal for 3, 7, 14, 21 and 24 d, respectively. The avidin-biotin complex （ABC） method demonstrated an accumulation of positively stained primitive mesenchymal cells at the fracture site in the hematoma stage of bone repair. These cells appeared to undergo differentiation into positively-stained chondroblasts and osteoblasts during the phase of callus formation. Undifferentiated mesenchymal cells showed a high positive reactivity in the early post-fracture stages but a much lower reactivity during the remodelling phase.The results of our study suggest that bone inductive processes are accompanied by the presence of BMP in osteoprogenitor cells during fracture healing of the mandible and that BMP may play a significant role in osteogenesis during bone healing.4|18|8Updated：2024-04-10
- Abstract：The responses of choriocapillary endothelial cells（CCE） and retinal capillary endothelial cells（RCE） in culture,in terms of phosphoinositide（PI） breakdown and cellular mitogenesis, to retinal pigment epithelial cell（RPE）-conditioned medium and vasoactive agents have been compared. RPE-conditioned medium did not induce PI breakdown in either type of cell. However, it stimulated DNA synthesis in CCE but not in RCE. Bradykinin（BDK） acted as both a fast signaling and a slow mitogenic factor on CCE, but BDK did not affect PI turnover or DNA synthesis in RCE. In contrast, thrombin stimulated PI turnover in RCE but not in CCE, though it did not induce 3H-thymidine incorporation into either type of cell. These differences in cellular functions between CCE and RCE following stimulation suggest that induction of DNA synthesis and receptor-mediated PI turnover by external factors is determined, at least in part, by the origin of the capillary endothelial cell. Therefore, extrapolation to CCE pathophysiology from experiments using endothelial cells from other capillary origins may not be valid.4|5|0Updated：2024-04-10
- Abstract：Injectable bovine collagen has been used clinically for years. But both the necessity of repeated injections to maintain corrections and the question of adverse allergic reactions developing from the use of a xenogenic collagen have been an area of serious concern. To overoome these adverse effects, we have developed injectable collagen preparations from human placenta. Gamma irradiation was used for sterilization and crosslinkins of the collagen.We observed the mouse immune respose to gamma-irradiated human placenta soluble and insoluble collagen following multiple injections. After six injections of these materials, no total IgG level increase was found, nor was antibody specifically directed against human collagen found.Mouse antibody levels were also observed following Zyderm II and Zyplast repetitive injections and following repetitive implantations of coated vicryl and chromic gut. No humoral immune response was found in this heterologous type system.4|7|0Updated：2024-04-10
- Abstract：ＳｔｕｄｙｏｆＬｕｎｇＣａｎｃｅｒｉｎＹｕｎｎａｎＴｉｎＣｏｒｐｏｒａｔｉｏｎＭｉｎｅｒｓ￥ＷａｎｇＸｉｅｈｕａ（王燮华）；ＺｈａｎｇＪｉｎｇｙｕａｎ（张景源）；ＨｕａｎｇＸｉｎｈｕｉ（黄星辉）；ＪｉａｎｇＨｕｉｘｉａ（姜会侠）；ＤｏｎｇＹｏｎｇｌｉ...4|5|0Updated：2024-04-10
- Abstract：Color Doppler flow imaging（CDFI） was performed in 24 patients with Graves’ disease and the results were compared with those from 8 patients with Hashimoto’s disease, 6 patients with simple diffuse goiter, and 15 normal volunteers. All cases were confirmed by clinical, laboratory tests or pathology. CDFI of Graves’ disease showed diffuse or localized hypoechoes within the thyroid glands, rich flow signals in the hypoechoes, accelerated flow velocity, and decreased resistance in the superior thyroid arteries（STA） and arteries within the glands. These appearances were characteristic as compared with the controls. The rate of flow in STA of Graves’ disease patients was 8 to 10 times higher than that in the normal volunteers. This study demonstrates that CDFI is convenient and efficient as compared with isotope scan and some other laboratory tests, and can be widely used in the diagnosis of Graves’ disase.4|19|1Updated：2024-04-10
- Abstract：To investigate the function of HLA-class Ⅱ genes in the autoimmune response of insulin dependent diabetes mellitus（IDDM） , the HLA-class Ⅱ gene of IDDM patients was introduced into Ltk-cells with pSV2-neo plasmid,using the calcium phosphate precipitation technique. We obtained a stable cell line expressing the HLA-class Ⅱ gene from lymphocytes of IDDM patients. Expression was identified by direct ox erythocyte-CrCl3-HLA DR monoclonal antibody rosetting.4|4|0Updated：2024-04-10
- Abstract：The multiglycosides of Tripterygium uilfordii（TII）, a ready-made Chinese herbal medicine used for the treatment of rheumatoid arthritis , have been shown to cause oligospermia in patients. In the present study, the antifertility effects of TII and tripchlorolide （T4, isolated from TII） were observed in male rats. In rats fed with TII at a dose of 10mg·kg·d for 7 weeks, the seminiferous tubules were essentially not influenced. However, most of the sperm heads along the surface of the tubular lumen were transformed from the normal sickle-shaped to round shaped. suggesting a possible mutagenic action. There was minimal testicular change but prominent epididymal spermatozoa damage in all rats treated with T4 （0. 05 mg·kg·d） for 7 weeks. The epididymal spermatozoa showed various structural abnormalities, including disrupted connecting pieces and cracked midpieces. and more than 80% of the spermatozoa were decapitated. No significant changes were seen in the main visceral organs. The data suggest that T4 may have good prospects as a male contraceptive.4|12|1Updated：2024-04-10
- Abstract：A case-control study involving 203 cases of pelvic endometriosis seen from 1987- 1989 , and 406 randomly selected and age-matched community controls was conducted in order to provide information relevant to effective prophylxais of the disease. The diagnosis was confirmed by pathology from laparotomy and/or laparoscopy. A questionnaire focused on menstrual, marital and reproductive status , professional exposure and physical activities ,and the results were analyzed by a conditional logistic regression model. Women characterized by earlier menarche（≤ 12 years） and longer period（≥8 days） were found to be associated with an elevated incurring risk, and a trend of increasing risk associated with primary dysmenorrhea （RR =2. 1 for mild to moderate and RR= 5. 2 for severe dysmenorrhea）, energetic physical activity during menstruation （RR =2. 1） , and allergic diathesis（RR=1 . 8） was seen. An inverse relationship was observed between the number of pregnancies and risk of endometriosis,and the protective effect was most significant when only the number of full-term pregnancies was counted.The risk factors of endometriosis are discussed, and intensive treatment of primary dysmenorrhea and avoidance of strenuous exercise during menstruation are identified as important measures in the prevention of endometriosis.4|15|1Updated：2024-04-10
- Abstract：ＴｈｅｒａｐｅｕｔｉｃＯｂｓｅｒｖａｔｉｏｎｓｏｆＴｉｇａｓｏｎｉｎＰｓｏｒｉａｓｉｓａｎｄＳｅｖｅｒｅＫｅｒａｔｏｔｉｃＤｅｒｍａｔｏｓｅｓ￥ＷａｎｇＪｉａｂｉ（王家璧）(ＤｅｐａｒｔｍｅｎｔｏｆＤｅｒｍａｔｏｌｏｇｙ，ＰＵＭＣＨｏｓｐｉｔａｌ，Ｃ...4|8|0Updated：2024-04-10
- Abstract：The functional differentiations of stomach cancer specimens from 121 patients were investigated by enzyme-,mucin-,affinity-and immunohistochemical methods, and the stomach cancers were divided into five functionally differentiated types : 1 ) Absorptive Function Differentiation Type （AFDT）, 19. 8% ; 2) Mucin Secreting Function Differentiation Type（MSFDT） , 24. 0% ; 3) Absorptive and Mucin-Producing Function Differentiation Type（AMPFDT） , 47. 1%; 4 ) Special Function Differentiation Type（SFDT） , 0. 8 %; and 5) Non-Function Differentiation Type（NFDT） , 8. 3%. The results indicate that stomach cancer tissues of the same histological type often display differing functional differentiation, and these functionally differentiated types have different invasive and metastatic characteristics. In addition, the functionally differentiated types have particular organic affinities of metastasis and different clinical prognoses. This study suggests that this new functional classification may supplement histological classification. The mechanisms of liver and ovary metastases of stomach cancer are also dis-cussed.4|9|0Updated：2024-04-10
- Abstract：We investigated the effects of mouse recombinant IL-4 on hematopoiesis in vitro and in vivo. IL-4 alone was found to be incapable of stimulating colony formation, but it inhibited both IL-3-and GM-CSF-induced colony formation by murine hematopoietic progenitor cells. In contrast, colony formation induced by G-CSF was enhanced in the presence of IL-4. We also studied the influence of IL-4 on hematopoietic reconstiution after allogeneic bone marrow transplantation in a murine medel, and found that IL-4 had significant inhibitory effects on neutrophil recovery and that neutrophil recovery accelerated by IL-3 and G-CSF was significantly suppressed by IL-4. The combination of IL-4 and GM-SF caused a significant decrease in the absolute number of neutrophils.4|10|0Updated：2024-04-10
- Abstract：ＳＬＥＣｏｍｐｌｉｃａｔｅｄＭｏｎｏｃｌｏｎａｌＧａｍｍｏｐａｔｈｙｏｆＵｎｄｅｔｅｒｍｉｎｅｄＳｉｇｎｉｆｉｃａｎｃｅ￥ＹａｏＳｈａｏｗｕ（姚少吾）ａｎｄＺｈｕＪｉｎｇｊｉｎｇ（朱晶晶）(ＡｆｆｉｌｉａｔｅｄＹｕｅＹａｎｇＨｏｓｐｉｔａｌ，Ｓｈａｎ...4|6|0Updated：2024-04-10
- Abstract：ＧＥＭＦＩＢＲＯＺＩＬＩＮＤＵＣＥＤＭＹＯＳＩＴＩＳ：ＡＣＡＳＥＲＥＰＯＲＴＷＩＴＨＬＩＧＨＴＭＩＣＲＯＳＣＯＰＩＣＡＮＤＵＬＴＲＡＳＴＲＵＣＴＵＲＡＬＳＴＵＤＹ￥ＬｏｕｉｓＴｓｕｎ－ｃｈｅｕｎｇＣｈｏｗ；ＭＲＣＰａｔｈ；ＦＲＣＰＡ；ＦＡＣＡａｎｄＷ...4|6|0Updated：2024-04-10
- Abstract：Seventy patients with left atrioventricular accessory pathways and paroxysmal supraventricular tachycardia（PSVT） underwent radiofrequency catheter ablation（RFCA）. The success rate was 94. 3%. Among these patients,26 had manifest preexcitation syndrome, and 44 had concealed preexcitation. Eighteen patients with concealed preexcitation underwent coronary sinus （CS） pacing, and delta wave appeared in 15. The keys to successful RFCA were correct positioning of the radiofrequency（RF） catheter tip, A/V amplitude ratio, AV interval （in sinus rhythm） and VA interval（during SVT or ventricular pacing）. After 1～14 months of follow-up. two patients had supraventricular tachycardia（SVT） recurrence.4|8|0Updated：2024-04-10
- Abstract：ＡＲＥＰＯＲＴＯＦ３９ＣＡＳＥＳＯＦＢＲＯＮＣＨＩＯＬＯＡＬＶＥＯＬＡＲＣＡＲＣＩＮＯＭＡ￥ＲｅｎＨｕａ（任华）；ＬｉＺｅｊｉａｎ（李泽坚）；ＺｈａｎｇＺｈｉｙｏｎｇ（张志庸）；ＳｕｎＣｈｅｎｇｆｕ（孙成孚）ａｎｄＸｕＬｅｔｉａｎ（徐乐天）(Ｄｅｐａ...4|4|0Updated：2024-04-10
- Abstract：ＩｎｖｅｓｔｉｇａｔｉｏｎｏｆＢｌｏｏｄＴｒａｃｅＥｌｅｍｅｎｔｓｉｎＡｃｕｔｅＬｅｕｋｅｍｉａＰａｔｉｅｎｔｓｂｙＰＩＸＥ￥ＬｉｕＧｕｏｄｏｎｇ（刘国栋）；ＱｉａｎＬｉｎｓｈｅｎｇ（钱林生）；ＬｉｎＨａｎ（林汉）；ＳｈａＹｉｎ（沙因）；ＬｉｕＰｉｎ...4|7|0Updated：2024-04-10
- Abstract：ＶａｒｉａｔｉｏｎｏｆＬｅｆｔＶｅｎｔｒｉｃｕｌａｒＤｉａｓｔｏｌｉｃＦｉｌｌｉｎｇｉｎＤｏｐｐｌｅｒＥｃｈｏｃａｒｄｉｏｇｒａｐｈｉｃＰａｃｉｎｇＳｔｒｅｓｓＴｅｓｔａｎｄＩｔｓＶａｌｕｅｉｎＤｅｔｅｃｔｉｎｇＣｏｒｏｎａｒｙＡｒｔｅｒｙＤｉｓｅａ...4|5|0Updated：2024-04-10
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