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Volume ， Issue 3 ， 1994
- Abstract：Four （CA）n repeats, located in introns 44, 45, 49 and 50 of the dystrophin gene., were evaluated in Chinese. These loci are highly polymorphic, with polymorphism information contents of 0. 872, 0. 772, 0. 870 and 0. 718, respectively. All four loci can be easily amplified and labelled using two duplex PCR reactions with α-32P-dCTP and can be detected by denaturing polyacrylamide gel electrophoresis. Using these four loci and the two polymorphic （CA）n repeats located at the 5’ and 3’ ends of the dystrophin gene, we have developed a new PCR-based procedure -Amp-FLP （amplified fragment length polymorphism） linkage analysis for the gene diagnosis of DMD/BMD. This method can detect intragenic recombination rapidly and efficiently and greatly. improves the success rate of carrier detection and prenatal diagnosis in non-deletion DMD/BMD families. All of the loci used in this procedure are intragenic. In addition, the loci in introns 44. 45, 49 and 50 are located in the deletion-prone region of the dystrophin gene, making them valuable and useful in the identification of deletion mutations. Here we report one case of deletion detection using these four loci.4|20|1Updated：2024-04-10
- Abstract：O6-methylguanine-DNA methyltransferase （MGMT） gene expression in 6 Mer+ （HeLa S3, SMMC-7721,SGC-7901, B-239, AGZY83-a, and Cc80 1） and 2 Mer- （SHG-44 , and HeLa MR） human tumor cell lines was examined. Southern blot analysis revealed no deletion, amplification, or rearrangement of the MGMT gene in these cell lines. However ,～ 1. 0 kb transcripts were detected in the 6 Mer+ cell lines but not in the 2 Mer-cell lines by Northern blot analysis. Furthermore,a rough correlation between MGMT activity and mRNA level in these cell lines was observed. These results suggest that transcriptional regulation of the MGMT gene is the molecular basis of the absence of MGMT activity in Mer-cell lines.4|14|2Updated：2024-04-10
- Abstract：ＣｌｉｎｉｃａｌＡｐｐｌｉｃａｔｉｏｎｏｆＣｏｍｐｏｕｎｄＴｅｒｒａｍｙｃｉｎＬｏｚｅｎｇｅＺｈａｎｇＣｈｕｎｓｈｅｎｇ（张春生）(ＤｅｐａｒｔｍｅｎｔｏｆＯｔｏｒｈｉｎｏｌａｒｙｎｇｏｌｏｇｙ，ＳｈａｎｇｈａｉＰｏｗｅｒＨｏｓｐｉｔａｌ，Ｓｈａｎｇ...4|23|0Updated：2024-04-10
- Abstract：Antisetum against purified cytoplasmic granules from rat LCL tumor cells. and protein A-gold immunoelectron microscopy were used to study the secretory events in lysis of YAC-1 tumor cells by rat LCL tumor cells or by isolated LGL from normal rats. After 30 min incubation of effector and target cells together, gold-labeled cytoplasmic granules were often seen concentrated in the area of the LGL adjacent to the bound YAC-1 target. Within 60 min, the granules were observed to move to the cell border near the conjugated site. At this point, the granules were fused with the cell membrane. and subsequently released the gold-labeled contents into the junction between the LGL and the target cell. Gold particles could be seen at the E-T interface, on the LGL surface, or sometimes on the target cell surface. These data provide direct evidence for the hypothesis that under conditions of active cytotoxicity, natural killer cells secrete their cytoplasmic granule contents leading to the deposition of granule material on the target cell surface and the eventual lysis of the cell.4|2|0Updated：2024-04-10
- Abstract：There may be a close relationship between myc oncogenes and carcinogenesis of human neuroblastoma. In previous studies, we were able to induce differentiation of certain neuroblastoma cell lines with NGF. In order to study gene regulation during differentiation, N-myc and c-myc cDNA probe were hybridized with RNA extracted from different cell lines before and after NGF treatment. It was found that cell lines which expressed N-myc did not express c-myc while those with c-myc did not express N-myc except for SHEP cell line which had neither c-myc nor N-myc expression. In NGF-induced differentiated neuroblastoma cells, c-myc oncogene was down-regulated in comparison with the control samples. The time course of c-myc down-regulation was concomitant with the appearance of morphological differentiation. In situ hybridization also showed remarkable reduction of c-myc oncogene expression in NGF-induced differentiated cells as compared with the untreated control cells. These results indicate that down-regulation of c-myc oncogene may be a key event during NGF-induced differentiation and overexpression of c-myc oncogene may, at least partially, be responsible for the genesis of neuroblastoma.4|9|1Updated：2024-04-10
- Abstract：ＢｒａｉｎｓｔｅｍＡｕｄｉｔｏｒｙＥｖｏｋｅｄＰｏｔｅｎｔｉａｌｓｉｎＤｅｖｅｌｏｐｉｎｇＣｈｉｌｄｒｅｎＺｈａｎｇＹｏｎｇｈｏｎｇ（张永红）；ＸｉｅＸｕｅｋｏｎｇ（解学孔）ａｎｄＣａｉＢａｉｙｕａｎ（蔡佰元）(ＴｈｅＮｅｕｏｌｏｇｉｃａｌＲｅｓｅａ...4|6|0Updated：2024-04-10
- Abstract：Reciprocal interactions between hemopoietic stromal cells and immature hemopoietic cells in human spleens obtained from 20 fetuses of 10-28 weeks gestation were observed by transmission electron microscopy and scanning electron microscopy. The close association of stromal cells with immature hemopoietic cells was confirmed under the electron microscope and a presumptive HIM （Hemopoietic inductive microenvironment） was visualized. In regions of immature hemopoietic cell-reticular cell, endothelial cell, macrophage and interdigitating cell contact,some communicating structures were found between the plasma membranes of adjacent cells; moreover, the cytoplasm of these four stromal cells were full of various kinds of organelles. These results suggest that reticular cells,endothelial cells, macrophages and interdigitating cells are component parts of the HIM of human fetal spleen and that these cells have a nurturing function in relation to hemopoietic cells.4|9|0Updated：2024-04-10
- Abstract：The present study was to investigate changes of free radicals in the whole rat liver, changes of ATP levels of hepatic cells, ultrastructural changes in hepatic tissue during ischemia and reperfusion in rats and the effects of ligustrazine and salvia compound. The results indicated that : 1 ) the free radical levels increased by 8. 3-fold and 9.0-fold in the groups with 30 and 60 min ischemia after 40 min reperfusion, as compared with the group with ischemia only （P<0.01, P<0.01, respectively）. 2) The levels of ATP returned to normal in the group with 30 min ischemia after reperfusion for 40 min and 3 d , remained low in the group with 90 min ischemia and fell again after a mild increase in the group with 60 min ischemia. 3) The hepatic and endothelial cell damage after ischemia became more severe after reperfusion, as revealed by electron microscopy. The presenet study also showed that ligustrazine and salvia compound have protective effects against reperfusion injury. They can be used to scavenge free radicals, improve hepatic microcirculation and alleviate hepatic and endothelial cell damage.4|15|0Updated：2024-04-10
- Abstract：Lipopolysaccharide （LPS） was found to induce significant hepatocytotoxicity against cultured mouse hepatocytes. Degeneration and necrosis of cultured hepatocytes and decrease of hepatocyte viability were prominent. The aspartate transferase level and 3H-TdR release in the medium were significantly increased after treatment, and the degree of these changes paralleled with LPS concentration. Various other parameters showed no significant difference between the hepatocytes cultured alone and those cocultured with nonparenchymal liver cells. However, if the nonparenchymal liver cells were isolated from mice which had been pretreated with D-galactosamine （GalN） not only was the hepatocyotoxicity induced by LPS enhanced, but the cells also showed certain cytotoxicity against cultured hepatocytes even without LPS, These results suggest that nonparenchymal liver cells might promote LPL-induced hepatocyte injury.4|8|1Updated：2024-04-10
- Abstract：Sixty-five patients with neoplasm （62 cases） or pancreatitis （3 cases） were treated with preserving pylorus pancreatoduodenectomy （PPPD） from 1984 to 1991. One postoperative death occurred. Follow-up studies were performed in 35 patients who had been treated by PPPD or the standard Whipple’s procedure; they were questioned carefully concerning clinical symptoms. Further studies were porformed in 20 patients with or without pylorus preservation （10 patients, respectively）. Nutritional status and gastrointestinal digestive and absorptive functions were evaluated by detemination of serum components, gastric analysis, barium emptying time, D-xylose absorption test,14CO2 breath test, PABA, and other methods. The results demonstrated malnutrition and postgastrectomy syndromes in some patients after the standard Whipple’ s procedure, but not in those with PPPD. The quality of life was better in the latter. Pylorus preservation may be the main reason for this above difference. Delayed gastric emptying in the early postoperative period was a complication in some patients（21%） treated by PPPD.We recommend PPPD for pancreatoduodenectomy.4|11|0Updated：2024-04-10
- Abstract：ＤｅｔｅｒｍｉｎａｔｉｏｎａｎｄＡｎａｌｙｓｉｓｏｆＢｏｎｅＭｉｎｅｒａｌＤｅｎｓｉｔｙｏｆｔｈｅＵｌｎａａｎｄＲａｄｉｕｓｉｎ８０ＰａｔｉｅｎｔｓｗｉｔｈＮｏｎ－ＩｎｓｕｌｉｎＤｅｐｅｎｄｅｎｔＤｉａｂｅｔｅｓＭｅｌｌｉｔｕｓｉｎＨａｎｇｚｈｏｕＧ...4|7|0Updated：2024-04-10
- Abstract：An 88-year-old man presented symptoms and signs of ascending cholangitis and died 20 days after the onset of illness. Postmortem examination revealed a mucinous tumor arising from the head of the pancreas, encasing the common bile duct and invading the liver with multiple hepatic metastasis. The tumor showed a unique and uniform histological apperarance, consisting of signet-ring neoplastic cells floating in mucin pools. The rapid clinical course and widespread hepatic metastasis of this patient suggest that this pure, signet-ring variant of mucinous adenocarcinoma of the pancreas might have a poorer prognosis.4|21|2Updated：2024-04-10
- Abstract：ＣｈｒｏｍｏｓｏｍａｌＬｏｃａｌｉｚａｔｉｏｎｏｆＨｕｍａｎＨｅａｔＳｈｏｃｋＰｒｏｔｅｉｎ９０βＧｅｎｅＳｈｉＹｉｊｉａｎｇ（施一江）；ＣｈｅｎｇＺａｉｙｕ（程在玉）；ＤａｏＣｈｕｎｓｈｅｎｇ（高春生）；ＬｉｕＪｕｈｏｎｇ（刘巨洪）ａｎｄＳｈｅｎＹ...4|5|0Updated：2024-04-10
- Abstract：This is a study of 18 patients who had surgical treatment for spinal tuberculosis. All patients were admitted with the spinal tuberculosis and treated with a combination of anterior debridement and bone graft with poterior internal fixaton using the Luque or Dick technique. The patients were able to walk within a few days postoperation. Three to seven years follow-ups show no recurrence. kyphosis and/or solid fusinon.4|12|1Updated：2024-04-10
- Abstract：ＴｈｅＣｒｏｓｓ－ＬｉｎｋｉｎｇＥｆｆｅｃｔｓｏｆＨｙｄｒｏｘｙｌＣｈｒｏｍｉｕｍｏｎＢｏｖｉｎｅＰｅｒｉｃａｒｄｉｕｍＪｉｎＬｅｉ（金磊）；ＺｈｕＸｉａｏｄｏｎｇ（朱晓东）；ＳｏｎｇＬａｉｆｅｎｇ（宋来凤）；ＣｈｅｎＬａｎｙｉｎｇ（陈兰英）ａｎｄＺ...4|17|0Updated：2024-04-10
- Abstract：Pulmonary arterial pressure （PAP） was increased obviously in rats after 3 days of normobaric hypoxic exposure and reached a maximum at 14 days of hypoxia. It remained at the same level during prolonged hypoxic exposure of up to 21 days. Right ventricular weight （RV/LV +S） and hydroxyproline （HP） content in the pulmonary artery began to increase at day 7. HP content had increased much faster than the relative rate of increase of PAP after 14 days, but HP content in the thoracic aorta showed no change. The relative proportion of type Ⅰto Ⅲ collagen increased singnificantly, and compliance of the pulmonary vessels obviously decreased. All parameters returned to the normal range within 14 days after recovery from hypoxia, except for HP content as expressed per vessel. 764-3 treatment obviously attenuated most of the changes caused by hypoxia, though it had no effect on compliance of the pulmonary vessels. It is suggested that collagen, especially type Ⅰcollagen, accumulation may play an important role in maintaining pulmonary hypertension. 764-3 has certain protective effects and may be useful in the treatment of early chronic obstructive pulmonary disease.4|8|0Updated：2024-04-10
- Abstract：From December 1991 to April 1993, we performed color Doppler flow imaging （CDFI） in 11 patients with parathyroid adenoma, and all cases were confirmed by operation and pathology. In all the parathyroid adenomas,vessels were clearly revealed at the periphery of the upper pole and/or anterior periphery, where arterial signals were elicited. These arteries had branches into the adenomas and originated from inferior thyroid arteries on the same side in most cases. The internal flow signals were increased markedly as compared to normal thyroid, and high-velocity arterial signals were detected. Because of the thyroid’ s rich blood supply and landmark peripheral vessels, CDFI can distinguish parathyroid foci from thyroid nodules, lymph nodes, and normal tissues and provide a sound basis for the diagnosis of small parathyroid foci.4|12|0Updated：2024-04-10
- Abstract：One hundred and twenty-five patients with cardiovascular disease were examined by biplanar transesophageal echocardiography （BTEE）, including 67 with rheumatic heart disease （7 monitored during operation）, 22 with aortic diseases, 20 with congenital heart diseases. and 16 with other kinds of heart disease. The results showed that BTEE was not influenced by corpulent and pulmonary emphysema and was better than transthoracic echocardiography at imaging the interatrial septum, left atrium and left atrial appendage, thoracic aorta, and mitral artificial valve and at intraoperative monitoring. BTEE was also better than single-plane transesophageal echocardiography,because BTEE could be used to observe the heart and thoracic aorta in transverse and longitudinal planes, thus enlarging the transmission "window" as well as allowing the entire lesion to be imaged.This study suggests that BTEE will have good prospects in clinical application.3|4|0Updated：2024-04-10
- Abstract：Using 3 non-invasive tests, abnormalities of cardiovascular reflex function were found in 7 of 15 patients with achalasia. Abnormalities of heart rate responses to the Valsalva maneuver, deep breathing, and standing were noted in patients with autonomic neuropathy defect. The findings are consistent with the hypothesis that an abnormality of vagal function may contribute to the pathogenesis of achalasia.4|7|0Updated：2024-04-10
- Abstract：ＴｈｅＳｃｒｅｅｎｉｎｇｏｆＣｏｌｏｒｅｃｔａｌＣａｎｃｅｒｂｙＥｎｚｙｍａｔｉｃＤｅｔｅｃｔｉｏｎｏｆＳｉａｌｉｃＡｃｉｄ－ｆｒｅｅβ－Ｄ－Ｇａｌ（１→３）－Ｄ－ＧａｌＮＡｃｉｎＲｅｃｔａｌＭｕｃｕｓＭｕＣｈｕａｎｊｉｅ（穆传杰）；ＬｉＳｈｉｓｈｅ...4|4|0Updated：2024-04-10
- Abstract：Fractures are one of the most common causes of disability in older women. The quantity and density of bone decreases with age. Most types of fractures increase as bone density declines. But most of the knowledge about causes and prevention of fractures comes from studies performed in Western countries. Asian women appear to have similar or slightly lower bone density that may be a result of their smaller size. They appear to have a lower risk of hip fracture than Whites, which may be a result of their shorter hip axis. The risks of other types of fractures in Chinese women is less well defined and reasons for differences in the rates of osteoporotic fractures between China and Western countries remain to be explored. A study is underway in Beijing to describe the risks and potential causes of fractures among older women in urban China. Randomized trials in Western countries have demonstrated that calcium and vitamin D, estrogen, calcitonin,or bisphosphonates can reduce the rate of fractures. Increased intake of calcium and vitamin D may be the most effective approach to preventing fractures in China, but this should be tested in a randomized trial.4|24|2Updated：2024-04-10
- Abstract：ＨｉｓＢｕｎｄｌｅＣｏｎｄｕｃｔｉｏｎＷｉｔｈｏｕｔａｎＨＤｅｆｌｅｃｔｉｏｎＤｅｎｇＺｈａｏｗｅｎ；（邓昭文）ＨｕａｎｇＺｉｚｈｅｎ；（黄自珍）ＹｅＨｕｉｙｉｎｇ；（叶慧膺）ａｎｄＬｉａｎｇＹａｏｒｏｎｇ（梁耀荣）(ＤｅｐａｒｔｍｅｎｔｏｆＭｅｄｉ...4|6|0Updated：2024-04-10
- Abstract：Ｅｆｆｅｃｔｓｏｆ７６４－３ｏｎｔｈｅＡｎｔｉｃｏａｇｕｌａｔｉｏｎＦｕｎｃｔｉｏｎｏｆＨｕｍａｎＶａｓｃｕｌａｒＥｎｄｏｔｈｅｌｉａｌＣｅｌｌｓＬｉＦｕｇａｎｇ（李福刚）；ＷｅｎｇＪｉｎ（翁进）ａｎｄＷａｎｇＺｈｏｎｇ（汪钟）(Ｉｎｓｔｉｔｕｔｅｏ...4|7|0Updated：2024-04-10
- Abstract：ＶａｒｉａｔｉｏｎｏｆＬｅｆｔＶｅｎｔｒｉｃｕｌａｒＤｉａｓｔｏｌｉｃＦｉｌｌｉｎｇｉｎＤｏｐｐｌｅｒＥｃｈｏｃａｒｄｉｏｇｒａｐｈｉｃＰａｃｉｎｇＳｔｒｅｓｓＴｅｓｔａｎｄＩｔｓＶａｌｕｅｉｎＤｅｔｅｃｔｉｎｇＣｏｒｏｎａｒｙＡｒｔｅｒｙＤｉｓｅａ...4|5|0Updated：2024-04-10
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