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Volume ， Issue 4 ， 1994
- Abstract：CD-1, a genetically-engineered CHO cell line, was cultivated with a Biosilon microcarrier culture system.We successfully cultivated CD-1 cells to a very high density （over1×107cells/ml）. Prourokinase was stably secreted at about 180 IU/ 1e6 cells/24 h. Experiments showed that CD-1 cells growing on Biosilon microcarriers were able to spontaneously release from the microcarriers, then reatthch and proliferate on fresh microcarriers. This makes it very easy to scale up preduction. The microcarriers could be reused several times without affecting adhesion. proliferation and prourokinase secretion. With CMPECC membrane radial flow chromatography and MPG chromatography, the prourokinase in conditioned medium could be purified to a specific activity of 1×105 IU/mg of protein. The purification factor was about 600 fold, and approxiamately 90 % of the biological activity was recovered.4|30|3Updated：2024-04-10
- Abstract：ＡＮｅｗＭｅｔｈｏｄＦｏｒＰｕｒｉｆｉｃａｔｉｏｎｏｆＡｎｔｉ－ＤＮＡＡｎｔｉｂｏｄｉｅｓ￥ＹａｎＸｉｙｕｎ闫锡蕴（ＩｎｓｔｉｔｕｔｅｏｆＭｉｃｒｏｂｉｏｌｏｇｙ，ＡｃａｄｅｍｉａＳｉｎｉｃａ），Ｂｅｉｊｉｎｇ１０００８０Ｔｈｅｐｕｒｉｆｉｃａｔｉｏ...4|9|0Updated：2024-04-10
- Abstract：Early diagnosis of leptospirosis of pulmonary diffuse hernorrhage type （PDH） is of crucial importance in saving patients. To develop a sensitive and specific methed for diagnosis, a genomic library of the main pathogen of PDH, L. interrogans serovar lai strain 017, was constructed with the plasmid vector PUC9. Recombinant plasmids which have hornologous fragments of pathogenic leptospires were screened from the bank. A recombinant plasmid,designated PCX7, could detect 1.7 kb fragment of strain 017, 9. 0 kb of strain 601 and 30. 0 kb of strain Hebdomadis, respectively, without cross hybridization with nonpathogenic leptospires such as L. biflexa strain Patoc I and hoptonema illini. The recombinant plasmid PCX7 could detect pathogenic leptospires which are the main pathogens endemic to Sichuan Province.4|4|0Updated：2024-04-10
- Abstract：In the present study, the effects of VIP on the growth of two human pancreatic carcinoma cell lines PU-PAN-l and PANC-I were determined using tritiated thymidine incorporation. VIP receptors. intracellular cAMP and polyamines were investigated. The results indicated that VIP at a concentration of 10-8 mol/L to 10-7 mol/L can significantly stimulate the growth of PU-PAN-I cells but not PANC-1 cells. This effect is dose-dependent and abolished by VIP receptor antagonist, [4-C1-Phe6 . Leu17] VIP, suggesting VIP receptors in PU-PAN-I cells may mediate this effect. VIP can markedly elevate the levels of intracellular cAMP and polyamines in PU-PAN-I cells.indicating that the growth-promoting effect stimulated by VIP may be via a rapid increase in the biosyntheses of cAMP and polyamines. In addition, the VIP-antibody inhibited the growth of PU-PAN-I cells in serum-free culture mediurn. The results above suggested that VIP has an autocrine regulatory effect on this pancreatic carcinoma cell line （PU-PAN-1）.4|5|1Updated：2024-04-10
- Abstract：ＴｈｅＲｅｌａｔｉｏｎｓｈｉｐＢｅｔｗｅｅｎＢｉｏｐｒｏｓｔｈｅｔｉｃＨｅａｒｔＶａｌｖｅＦａｔｉｇｕｅａｎｄＩｔｓＣａｌｃｉｆｉｃａｔｉｏｎｉｎＳｕｂｃｕｔａｎｅｏｕｓｌｙＩｍｐｌａｎｔｅｄＲａｔｓ￥ＪｉｎＬｅｉ金磊，ＺｈｕＸｉａｏｄｏｎｇ朱晓东Ｓ...4|9|0Updated：2024-04-10
- Abstract：This article reports the identification of 57 AFB cultures isolated from clinical specimens by using traditional methods （TM, including biochemical and cultural methods） and modern ELISA with monoclonal antibodly （McAb-ELISA） and nested primer gene amplification assay （NPCAA）. The tepresentive AFB culture M. A1 . A7, A19.A21 and A22) isolated from human lepromas were identified as new species by TM and it was shown that they were not identical to M. leprae by McAb-ELISA and NPGAA. Among another set of samples （M. S17 . S1, S2, S2R . S7,S29 ） . M. S17 was identical to M. scrofulaceum as assessed by TM only . while the others were found to be similar to M. tuberculosis and different from M. leprae using TM and McAb-ELISA, and identical to M. tuberculosis with NPGAA. The authors conclude that TM and MM are very useful for identifying mycobacteria, while MM was much more sensitive and specific than TM. The selection and use of these methods depends on practical need.4|7|0Updated：2024-04-10
- Abstract：ＴｈｅＴｒｅａｔｍｅｎｔｏｆＳｐｏｎｔａｎｅｏｕｓＰｎｅｕｍｏｔｈｏｒａｘ￥ＧｅＦｅｎｇ戈烽，ｗａｎｇＹｏｎｇｈｕａ王永华，ＬｉａｏＱｕａｎ廖泉，ａｎｄＬｉＺｅＪｉａｎ李泽坚（ＰＵＭＣＨｏｓｐｉｔａｌ，ＣＡＭＳ＆ＰＵＭＣ）．Ｂｅｉｊｉｎｇ１００００５...4|11|0Updated：2024-04-10
- Abstract：The aim of this study was to determine the time course of changes in extracellular fluid （ECF） concentrations of purines, amino acids, monoamines, and their metabolites in the striatum of rats during ischemia and reperfusion, using intracerebral microdialysis as the sampling technique. In rats subjected to 20 min forebrain ischemia by four-vessel occlusion, the concentrations of adenosine （Ade）, inosine （Ino） and hypoxanthine （Hyp） were found to rise markedly. These changes were accompanied by dramatically elevated levels of aspartate （Asp）, glutamate （Glu）, taurine （Tau）, γ-aminobutyric acid （GABA）, dopamine （DA） and norepinephrine （NE）, all of which gradually returned to baseline following reperfusion. Concomitantly, the levels of metabolite 3, 4-dihydroxyphenylacetic acid （DOPAC） . homovanillic acid （HVA）, 5-hydroxyindole-3-acetic acid （5-HIAA） and xanthine （Xan） decreased during ischemia and gradually recovered 60～ 90 min after reperfusion. It was concluded that during global brain ischemia, the ECF is flooded with both potentially harmful （e. g. Asp, Glu, DA） and protective （e. g. Tau, GABA, Ade） agents.4|18|0Updated：2024-04-10
- Abstract：ＵｌｔｒａｓｔｒｕｃｔｕｒａｌＤｙｎａｍｉｃＣｈａｎｇｅｓｉｎＲｅｇｅｎｅｒａｔｉｎｇＨｅｐａｔｉｃｃｅｌｌｓＦｏｌｌｏｗｉｎｇＰａｒｔｉａｌＨｅｐａｔｅｃｔｏｍｙｉｎＲａｔｓ￥ＨａｎＳｈｕｙｕａｎ（韩涉媛），ＨｕａｎｇＺｈｉｑｉａｎｇ（黄志强），Ｌ...6|5|0Updated：2024-04-10
- Abstract：The action of Gly-Tyr-NH2, （GY-NH2） and Gly-Tyr-LYS（GYK） on ￣（125）I-LH binding, cAMP accumulation and progesterone production was investigated. Incubation of rat luteal cells for 2.5 h with GY-NH2 and GYK at dosage of 0. 2 mmol/L caused a significant inhidition of basal and gonadotropin-stimulated steroidogenesis. GY-NH2 and GYK were also found to reduce cAMP formation in response to hCG. The activity of adenylate cycles of luteal cells was inhibitd by 0. 2 mmol/LGY-NH2 and GYK. GY-NH2 and GYK at a concentration of 0. 2mmol/L were not found to have an inhibitory effect on 8Br-cAMP-stimulated progesterone preduction. GY-NH2 and GYK did not affect ￣（125）I-LH binding to LH receptors on the luteal cell surface. These results suggest that GY-NH2 and GYK inhibit steroidogenesis at the step of gonadotropin-stimulated cAMP formation in luteal cells. Adenylate cyclase in luteal cells was also inhibited.4|5|0Updated：2024-04-10
- Abstract：This study explores a novel noninvasive method for monitoring blood alfentanil concentrations using a dog model. Alfentanil which "back", permeated across the oral mucosa from the systemic circulation was collected from the oral mucosal surface and quantitated. The levels of the "back" permeated alfentanil were found to closely reflect real time serum alfentanil concentrations. With further work, this finding may lead to a novel noninvasive method for monitoring real time serum alfentanil concentrations in its clinical applications.4|8|0Updated：2024-04-10
- Abstract：ＦｌｏｗＣｙｔｏｍｅｔｒｉｃＡｎａｌｙｓｉｓｏｆＣｅｌｌｕｌａｒＤＮＡＣｏｎｔｅｎｔａｎｄＩｔｓＲｅｌａｔｉｏｎｔｏＡｇｅＤｉｓｔｒｉｂｕｔｉｏｎｉｎＨｕｍａｎＢｒｅａｓｔＣａｎｃｅｒ￥ＬｉＪｉｖｅ（李基业）ａｎｄＺｈａｎｇＳｈｕａｎｇｘｉ（张双喜）...4|6|0Updated：2024-04-10
- Abstract：Cytokeratin （CK） 8 mRNA expression in developing and degenerating rat prostate was studied using in situ hybridization with an antisense RNA-probe. It was found that : 1) the CK 8 antisense probe was accumulated only within prostatic epithelial cells ; 2) after castration, CK 8 mRNA signals in ventral prostute（VP） sections were significantly increased, and elevated CK 8 mRNA expression persisted even long after prostate involution was complete; and 3) during prostate development,the strongest CK 8 mRNA staining was found in the early neonatal prostatic epithelia which were composed mainly of prostatic stem cells. Thereafter, a shift of CK 8 mRNA staining to peripheral regions and decreased overall CK 8 mRNA levels were noted. These data indicate that excessive expression of CK 8 mRNA is a characteristic of prostatic stem cells, and CK molecules are excellent markers for determing the hierarchical pathway of cell differentiation in prostate epithelium.4|12|0Updated：2024-04-10
- Abstract：For TBI （total body irradiation） prior to BMT（bone marrow transplantation ）and in order to guarantee exact treatment,it is necessary to perfect in vivo dosimetry to detect any deviation of the treatment and to verify the dose dis-tribution. A simplified and convenient transmission type in vivo dosimetry and problems are introduced and discussed.4|5|0Updated：2024-04-10
- Abstract：The effects of opioid peptides on iminune responses were investigated. It was found that β-endorphin （β-END） can depress proliferative responses to PHA in rat splenocytes but enhance those in mice, and it could also inhibit the plaque-forming cell （PFC） response to sheep red blood cells when mouse splenocytes immunized in vivo were cultured in vitro with the peptide. The peptide antagonist naloxone was able to reverse β-END suppression of the PFC response. The data indicate that β-END suppresses antibody production or secretion via a specific opioidreceptor-mediated mechanism.4|10|0Updated：2024-04-10
- Abstract：The characteristics of rhuIL-4 induced cytotoxicity was detected in vitro by using ￣（51）Cr release assay and the anti-tumor activity of rhuIL-4 induced killer cell was evaluated in vivo by using a human tumor model in nude mice. huIL-4 can induce LAK activity from peripheral blood lymphocytes （PBMC） stimulated with phytohemagglutinin （PHA）. Compared with the LAK activity induced by rhuIL-2, the cytotoxicity of the killer cells induced by rhuIL-4 to K562 and Raji cells was lower, but that to TBL-E, a human lymphoid leukernia cell line established in our laboratory, and PHA-activated blast cells （PHA-blasts） was of similar magnitude. In the cytotoxicity assay using PHA-blasts, the addition of PHA increased the IL-4-induced killer cell cytotoxicity by 131%, but had no effect on IL-2-induced killer cell cytotoxicity. This implies that IL-4 mainly induces CTL-like activity, while IL-2 mainly induces NK-like activity. An experimental human tumor model in nude mice was established by injection of TBL-E human leukemia cells. The anti-tumor activity of rhuIL-4 was evaluated by injection of human LAK cells induced from PHA-blasts by rhuIL-2+rhuIL-4 and human cytokines into tumor-bearing nude mice. The results showed that human LAK cells effectively inhibit the tumorigenicity of TBL-E cells in nude mice with an inhibition rate of 61 %. The antitumor effect of rhuIL-2 was better than that of rIL-4, and the antitumor effect of rhuIL-2+rhuIL-4 was similar to that of rhuIL-2, though the former delayed the occurence of tumors. Our data imply the potential application of human IL-4 in clinic, and provide an animal model to evaluate the anti-tumor activity of human cytokine（s） with species specificity.4|9|0Updated：2024-04-10
- Abstract：Chondromalacia and patellofemoral osteoarthritis are common diseases that cause pain and disablement of the knee. Conservative therapy is not always effective. Since 1983 we have used isolated polyethylene patellar prosthesis for patella resurfacing of 19 patients with patellofemoral osteoarthritis. After an average of 44. 9 months follow-up, we found the treatment was not as satisfactory as earlier trials. The main reason is the wide erosion of femoral condyle caused by the polyethylene patella. To overcome this shortness, we designed a new type of patellofemoral prosthesis which is named Y-L-Q. From January . 1991 to November. 1 993, we used this prosthesis to treat 16 knees of 13 patients with patellofemoral osteoarthritis. Most of those patients improved both symptomatically and functionally. The good to excellent results rate was 87. 5% （1 4/16 knees） at the time of an average 16 months follow-up. The early results of our experience with patellofemoral resurfacing are encouraging. And extended follow-ups are in progress.4|20|0Updated：2024-04-10
- Abstract：ＩＮＴＥＲＮＡＴＩＯＮＡＬＣＯＮＦＥＲＥＮＣＥＩＮＴＲＯＤＵＣＴＩＯＮ￥ＺｈａｎｇＢｉｔａｏ（张必涛）．ＰＵＭＣＨｏｓｐｉｔａｌ．ＢｅｉｊｉｎｇＴｈｅ１９９４ＡｓｉａｎａｎｄＰａｃｉｆｉｃｉｎｔｅｒｎａｔｉｏｎａｌＣｏｎｆｅｒｅｎｃｅｏｎＭｅｄｉｃａ...4|10|0Updated：2024-04-10
- Abstract：Four cases of anterior communicating aneurysms are reported with clinical presentations of recurrent intracranial hemorrhage undisclosed in their cerebral angiography. Operative exploration revealed anterior communicating aneurysms in these patients. It is thought possible that these patients harbored aneurysms which failed to exhibit on angiograms due to temporary thrombosis or development of abnormal cerebral vessels. CT scanning was important for diagnosis of these patients.4|26|0Updated：2024-04-10
- Abstract：ＩｓｃｈｅｍｉａａｎｄＲｅｐｅｒｆｕｓｉｏｎＲｅｄｕｃｅｓｔｈｅＥｎｄｏｇｅｎｏｕｓＢａｓｉｃＦｉｂｒｏｂｌａｓｔＧｒｏｗｔｈＦａｃｔｏｒｉｎＲａｔＳｋｅｌｅｔａｌＭｕｓｃｌｅｓ￥ＦｕＸｉａｏｂｉｎｇ（傅小兵），ＧｕｅｖａｓＰ，Ｇｉｍｅｎｅｚ－Ｇａｌ...4|9|3Updated：2024-04-10
- Abstract：During a 17-year period, 38 patients with primary tracheobronchial tumors received surgical treatment in PUMC Hospital. Arnong the 38. 12 had low-malignancy tracheal tumors, 11 had benign tracheal tumors, 14 had low malignancy bronchial tumors and 1 had a benign bronchial tumor. Fifteen operations were perforrned on 12 patients with low-malignancy tracheal tumors, including local resection of the tumor and tracheal wall in 4 and curettage of the tumor plus electrical cauterization in 10. Postoperative radiotherapy was used as an adjuvant treatment in 8 patients with adenocystic carcinoma. Eight patients have survived for more than 5 years and 3 patients have survived for longer than 10 years postoperatively. All 11 patients with benign tumors received curettage of the tumor and were followed up for an average of 6. 5 years. Among them, 9 are still alive. Of the 14 patients with low malignancy bronchial tumors, 5 underwent curettage of the tumor plus electrical cauterization through incision of the main bronchus or intermedial bronchus, and 7 underwent lobectomy or pneumonectomy. The authors conclude that the correct diagnosis rate can be increased by enhancing recognition of this disease and applying tracheal tomography and bronchoscopy.4|11|0Updated：2024-04-10
- Abstract：ＳＵＢＪＥＣＴＩＮＤＥＸＣＨＩＮＥＳＥＭＥＤＩＣＡＬＳＣＩＥＮＣＥＳＪＯＵＲＮＡＬＶｏｌｕｍｅ９．１９９４ＡＣＥｉｎｈｉｂｉｔｉｏｎＥｆｆｅｃｔｏｆＣｈｒｏｎｉｃＡＣＥＩｎｈｉｂｉｔｉｏｎｏｎＧｌｕｃｏｓｅＴｏｌｅｒａｎｃｅａｎｄＩｎｓｕｌｉｎＳｅｎ...4|4|0Updated：2024-04-10
- Abstract：ＥｆｆｅｃｔｓｏｆＭｅｄｉｃａｌＴｏｏｔｈｐａｓｔｅｏｎＤｅｎｔｉｎＰｅｒｍｅａｂｉｌｉｔｙｉｎＶｉｔｒｏ￥ＬｉｕＣｕｉｔｅｎｇ（刘翠凤）（ＰＵＭＣＨｏｓｐｉｔａｌ，ＣＡＭＳａｎｄＰＵＭＣ，Ｂｅｉｊｉｎｇ１００７３０）Ｉｎａｃｃｏｒｄａｎｃｅｗｉｔｈ...4|9|0Updated：2024-04-10
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