Latest Issue
Volume ， Issue 1 ， 1995
- Abstract：We present a novel approach for making cybrids. By introducing neo gene expression plasmids into rabbit reticulocytes, fusing the gene transferred reticulocytes with K562 cells and selecting in G418 selection medium, a cybrid strain K-RRneo was established. Whole mount TEM study demonstrated that after cybridization, there was a reorganization of the intermediate filaments which showed a tendency to differentiate towards reticulocytes. SDS-PAGE and western blot analysis verified the above observation, in which the vimentin blot pattern of the cybrids was similar to that of reticulocytes, but totally different from that of K562 cells. Using this model, we reaffirmed the hypothesis that the erythroid differentiation factor （EDF） might be responsible for erythroid differentiation as well as the initiation of denucleation.4|17|0Updated：2024-04-10
- Abstract：Investigations on the relationship between some tumor cell properties and their metastatic potential using different clones from two tumor cell lines are reported. CNE2L2（highly metastatic） and CNE2L4 （low metastatic） clones were isolated from human nasopharyngeal carcinoma CNE-2Z cell line. LA1. LAD and LA5 clones were isolated from mouse pulmonary adenocarcinoma LA795 cell line with different metastatic potentials. The results from both cell lines showed that the metastatic potential of tumor cell positively correlated with elecirophoretic mobility rate. adhesion with endothelial cells and in vitro and in vivo invasiveness. and negatively correlated with self-adhesion and adhesion to fibroblast cells. The data further confirmed the relationship between these factors and the metastatic potential of tumor cells.The results provide a theoretical basis for elucidating the mechanism of metastasis.4|9|0Updated：2024-04-10
- Abstract：ＳｙｎｔｈｅｓｉｓａｎｄＲａｄｉｏｓｅｎｓｉｔｉｚｉｎｇＥｆｆｅｃｔｓｏｆＩｎｄａｚｏｌｅｓＤｅｒｉｖａｔｉｖｅｓＷａｎｇＲｏｎｇｘｉａｎ（王荣先）；ＳｈｉＰｅｉｊｉ（施培基）；ＨｕＢｉ（胡壁）；ＲａｏＥｒｃｈａｎｇ（饶尔昌）；ＭｉＦｕｓｈｕｎ；(糜...4|12|2Updated：2024-04-10
- Abstract：In a retrospective study. liquid nitrogen preserved specimens from 50 women with primary breast cancer, who underwent surgery at the Beijing Institute for Cancer Research between June. 1986 and September, 1988, were investigated. All patients under this study were staged in TNM II or later, involved with axillary lymph node metastasis. and treated with systemic postoperative adjuvant chemotherapy. The median length of follow-up was 69 months. The expression of P-glycoprotein was investigated by means of immunohistochemistry, using a monoclonal antibody C219 specifically against P-glycoprotein and avidin -biotin peroxidase method. Posrtive staining for P-glycoprotein was found in 23（16%） of the 50 patients. The P-glycoprotein expression negative group fared better than the group that was P-glycoprotein posrtive in overall survival curves （p= 0. 0008 , by the generalized Wilcoxon test）. The prognostic effect of P-glycoprotein expression remained statistically significant （p=0. 0007） after adjustment by multivariate analysis（Cox’s model）for other prognostic factors. It is demonstrated that P-glycoprotein expression is a significant and independent Predictor of postoperative survival in breast cancer patients. The results of the present study suggest that P-glycoprotein expression might also influence the bioiogical behavior of breast cancers.4|15|1Updated：2024-04-10
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EFFECT OF GLYCYRRHETINIC ACID ON DNA DAMAGE AND UNSCHEDULED DNA SYNTHESIS INDUCED BY BENZO （α）PYRENE
Abstract：Glyeyrrhetinic acid （GA） is an active component of Glycyrrhiza uraleusis fisch,In this study,GA was found to inhibit ear edema and ornithine decarboxylase （ODC）activity induced by croton oil in mice. GA could also protect rapid DNA damage and decrease the unscheduled DNA synthesis induced by benzo（α）pyrene. The results demonstrate that GA has a potential cancer chemopreventive activity.4|18|1Updated：2024-04-10 - Abstract：Intact pZ189 DNA was replicated in monkey kidney vero cells which had been pretreated with Nmethyl-N’-nitro-N-nitrosoguanidine （MNNG） . The mutants were selected in E. coli MBM7070 and the mutation frequencies involving mutants with unchanged electrophoretic mobilrty of their plasmid DNA were scored. When compared to the spontaneous mutation frequency. the mutation frequencies were increased by 5.8 and 2.9-fold in cells pretreated with 0. 2 and 2μmol/L MNNG, respectively. The supF genes of these mutants were sequenced. and it was found that the types of base substitution and the sites of frameshifts differed from findings in studies of spontaneous and targeted mutagenesis. The results suggest that nontargeted mutagenesis occurs in mammalian cells and may have a sequence specificity.4|4|2Updated：2024-04-10
- Abstract：The effect of nucleoside diphosphate kinase （NDPK） on the activrty of guanine nucleotide regulatory protein （G-protein） mediated phospholipase C（PLC） and on the [35S]GTPTτS binding of G-protein was investigated in this work in order to demonstrate the mechanism behind the regulation of G-protein and its effector PLC by NDPK. The stimulation of PLC in turkey erythrocyte membrane by both GTP and GTPτS indicated that the PLC stimulation was mediated by G-protein. NDPK alone stimulated PLC activity. as well as the stimulation in the presence of GTP and GDP, in a dose-dependent manner. However. NDPK inhibited GTPτS-stimulated PLC. Furthermore, NDPK inhibited [35S]GTPτS binding of purified Gi-protein in a non-competitive manner. A hypothesis implying an important role of direct interaction of G-protein and NDPK in the regulation of their functions is suggested and discussed.4|12|0Updated：2024-04-10
- Abstract：Using immunoradiornetric TSH assay （TSH-IRMA） to measure whole blood TSH levels spotted onto filter paper, we compared TSH levels among different age groups （neonates, <1 yr infants, schoolchildren aged 7 to 14 yrs, adults,pregnant women and reproductive-age women） in Guiyang, where iodine deficiency has long been a problem. The results showed: 1) The percentage of subjects with TSH levels equal to or greater than 5 mIU/L in the neonate group was 38.9% while the percentages in the other groups were 0-3.3% （P<0.01）; 2)The TSH levels of the neonates were inversely related to the urinary iodine values of their mothers （pregnant women）. （r=-0. 5, P<0. 01）;3)97.6% of the inhabitants in Guiyang ingested salt with less than 20mg/kg iodine. The results indicate that iodine deficiency remains a problem in Guiyang. Neonates are the only ideal population for monitoring iodine deficiency.4|14|0Updated：2024-04-10
- Abstract：ＣｌｉｎｉｃａｌＲｅｖｉｅｗｏｆｔｈｅＮｕｔｒｉｔｉｏｎａｌＳｕｐｐｏｒｔｏｆＩｎｆｌａｍｍａｔｏｒｙＢｏｗｅｌＤｉｓｅａｓｅＣｈｅｎＴｉｎｇｙｕａｎ（陈亭苑）；ＹｕＫａｎｇ（于康）ａｎｄＺｈａｎｇＳｉｑｕａｎ（张思源）(ＰｅｋｉｎｇＵｎｉｏｎＭｅｄ...4|7|0Updated：2024-04-10
- Abstract：We report a simple and rapid HLA-DRBI generic typing method, PCRSSP, which is practical and inexpensive. We use 9 sequence-specific primers and 2 group specific Primers to define the HI-ADRB1 specificities DR1,DR2, DR3, DR4,DR5,DR6, DR7, DR8, DR9 and DR10. The HLA DR3, DR5,DR6 and DR8 can be amplified by the two primers of DR3568 and DRB1. The DR6 specificity can be identified by excluding the DR3, DR5 and DR8 when the DR3568 are positive. Any individuals can be typed with some exception: the three pairs of phenotype DR3/DR3 and DR3/DR6, DR5/DRS and DR5/DR6,DR8/DR8 and DR8/DR6 cannot be discriminated from each other. We typed 106 unrelated healthy people from Beijing locations in two weeks. We think this typing method is suitable to replace the error-prone serologic HLA-DR tests in routine clinical practice, including the Prospective typing of cadaveric organ donors.4|14|2Updated：2024-04-10
- Abstract：ＣｌｉｎｉｃａｌＳｔｕｄｙｏｆＢｌａｓｔｏｃｙｉｓａｓｉｓｉｎＣｈｉｌｄｒｅｎｏｆＦｕｚｈｏｕＤｉｓｔｒｉｃｔＷｕＢｉｎ（吴斌）；ＺｈａｏＺｉｑｉｎｇ（赵子庆）；ＨｕａｎｇＭｉａｏｈｕｉ（黄妙辉）ａｎｄＳｕＱｉｎｇｐｉｎｇ（苏庆平）(ＴｈｅｌｓｔＡｆ...4|6|0Updated：2024-04-10
- Abstract：The results of application of flow cytometry （FCM） in analysis of 139 bladder irrigations of DNA contents in tumor cells from 52 cases of bladder tumors are reported. A comparison was made between this assay and cytological examinarions as well as pathological grading. The results suggested that the accuracy of cytological analysis was apparently lower than the cell DNA measurement by flow cytometry,the higher the tumor pathological grading and the deeper the infiltration. the higher the heteroploid DNA contents in the tumor cells. Treatment of high heteroploid bladder tumor required radical cystectomy while the efficiency of simple electrosection or partial cystectomy appeared inferior. When FCM and the bladder tumorpathological grading had a high degree of correlation. especially in the high grading tumor, recurrence was high even when chemical treatment with bladder instillation of BCG was adopted. However, FCM cannot substitute for cystoscopy.4|3|0Updated：2024-04-10
- Abstract：ＳｔｒｕｃｔｕｒａｌＡｂｎｏｒｍａｌｉｔｉｅｓａｎｄＰｏｌｙｍｏｒｐｈｉｓｍｓｏｆｔｈｅＲｅｔｉｎｏｂｌａｓｔｏｍａＧｅｎｅｉｎＮａｓｏｐｈａｒｙｎｇｅａｌＣａｒｃｉｎｏｍａＬｏｎｇＪｉａｎｇｂｉｎ（龙江斌）；ＯｕＢａｏｘｉａｎｇ（区宝祥）；Ｌｉａｎ...4|4|0Updated：2024-04-10
- Abstract：A randomized block design was used to compare the Singh index, the cortical index of the femoral neck and the lateral cortical width of the femur of a group of patients with femoral neck fracture, a group with intertrochanteric fracture, and a normal control group among a total of 102 postmenopausal women.The three indices in fracture group were very significantly （P<0.01） or significantly （P<0.05） different from those in the normal control subjects. The importance of osteoporosis in hip fracture of the aged is con firmed. Radiographic measurement of bone mass was found to have some predictive value for the risk of hip fracture.4|12|0Updated：2024-04-10
- Abstract：Chorionic villi and fetal tissues from 50 pathological human conceptions at gestational weeks 9-40 were cultured and cytogenetically analyzed to explore the existence of chromosomal mosaicism confined to the extraembryonic tissues and to clarify the relationship between confined placental mosaicism and adverse outcome of pregnancy. Chorionic villi and fetal tissues from 12 second trimester gestations terminated for social reasons served as a control group. In two pathological gestations, true mosaicism was found exclusively in chorionic cells and could not be confirmed in cells derived from the fetal tissues. One of these was severely growth retarded. Concordant results were obtained in all other cases.4|5|0Updated：2024-04-10
- Abstract：ＰｌａｓｍａＦｉｂｒｏｎｅｃｔｉｎｉｎＮｏｒｍａｌＳｕｂｊｅｃｔｓａｎｄＰａｔｉｅｎｔｓｗｉｔｈＶａｒｉｏｕｓＤｉｓｅａｓｅｓＴａｎｇＤｅｊｉ（汤德骥）；ＰａｎｇＸｉｎｇｇｕａｎｇ（庞兴光）ａｎｄＬａｎｇＹａｍｉｎ（郎亚民）(ＩｎｓｔｉｔｕｔｅｏｆＨ...6|4|0Updated：2024-04-10
- Abstract：The phenomenon of cytogenetic adaptive and cross-adaptive response induced by low dose irradiation and chemical mutagen in mice is described. We found, firstly, that adaptation can be induced by acute low dose Xirradiation （0-100 mGy）. Secondly, a cross-adaptation can occur between X-irradiation and mitomycin C （MMC）. And finally, mice pre-exposed to chronic low dose rate￣（60）Co-Gamma irradiation （0-226.0 mGy/day） are less susceptible to chromosome aberration induced by subsequent acute higher Xirradiation. Therefore, our data suggest that radioadaptive response depends on dose, dose rate and time interval. Possible mechanisms are also discussed.4|8|0Updated：2024-04-10
- Abstract：One patient with wound surface sepsis caused by secondary pyocyanic infection after extensive burns associated with visceral injuries （peptic ulcer hemorrhage, renal insufficiency and hepatic dysfunction） and generalied candidiasis albicans was cured after anti-infection treatment with proper antibiotics, removal of the infected focus, and effective anti-fungal drugs.4|8|0Updated：2024-04-10
- Abstract：Twentysix Patients with sleep disturbances who had completed therapy with hypnotics were observed under single-blind and self-comparable method for 7 days each. We found that patients treated with imovane or estazolam had no statistical differences with respect to sleep time onset. total sleep time. nocturnal awakening. feeling after awakening, dream, drug side effects and sleep qualirty.Imovane performed better than estazolam in sleep time onset and total sleep time. From this study, imovane can he recommended as a new hypuotic to treat patients with sleep disturbances.4|9|0Updated：2024-04-10
- Abstract：ＳｙｎａｐｔｉｃＣｏｎｔａｃｔｓｏｆｔｈｅＣｅｎｔｒａｌＡｍｙｇｄａｌｏｉｄＮｕｃｌｅｕｓｉｎＳｐｏｎｔａｎｅｏｕｓｌｙＨｙｐｅｒｔｅｎｓｉｖｅＲａｔＡｎｄＮｏｒｍｏｔｅｎｓｉｖｅＷｉｓｔａｒＫｙｏｔｏＲａｔＡｎＸｉｎｈａｉ（安新海）ａｎｄＸｕＬｕｘ...4|6|0Updated：2024-04-10
- Abstract：In order to study the altered molecular events during laryngeal carcinogenesis and elucidate the role of Haras oncogene amplification and mutation. we have examined their profile by polymerase chain reaction （PCR） and selective oligonucleotide hybridization. We analyzed the mutational status of codon 12 of Haras in 22 laryngeal carcinomas and 10 normal tissues. and found that 7 of 22 laryngeal carcinomas contained a Ha-ras mutation at codon 12. The frequency of mutation was 32%. None of the normal tissues revealed mutation. Moreover. no amplification was found in cancers when compared to the normal. Ourfindings indicated that the activated Ha-ras gene existed in laryngeal carcinoma. and activation of the Haras gene by mutation at codon 12 might play a key role in laryngeal carcinogenesis.4|10|3Updated：2024-04-10
- Abstract：Between 1974 and 1993, 22 patients with bronchogenic cysts were operated on in our hospital; there were 14 men and 8 women, ranging in age from 11 to 62 years. The cyst locations were mediastinal in 13 （59.1%） and intrapulmonary in 9 （40. 9%）. There were symptoms （chest pain and recurrent bronchiolits） in 20 patients （91%）. The preoperative complications included infection in the lung and in the cyst and dysphagia due to esophageal compression. Chest pain was the main symptom in mediastinal cyst and recurrent infection of lung in intrapulmonary cyst. Plain chest radiograms showed that a round shadow, occasional air-fluid levels, and peripheral calcification may be found in cysts. An operation is the best treatment for cysts. All cysts were completely excised. No postoperative cornphcations. late complications, or recurrence developed in our patients.4|14|0Updated：2024-04-10
- Abstract：ＥｎｄｏｔｏｘｉｃＤａｍａｇｅｔｏｔｈｅＳｔｒｉａＶａｓｃｕｌａｒｉｓ：ＴｈｅＰａｔｈｏｇｅｎｅｓｉｓｏｆＳｅｎｓｏｒｉｎｅｕｒａｌＨｅａｒｉｎｇ ＬｏｓｓＳｅｃｏｎｄａｒｙｔｏＯｔｉｔｉｓ ＭｅｄｉａＧｕｏＹａｎｇｃｈｕｎ（郭养淳）；ＷｕＹｉｑｉｎ...6|10|0Updated：2024-04-10
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