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Volume ， Issue 3 ， 1995
- Abstract：This study focused on the influences of opioids on the generation of antibody against sheep erythrocyte in vitro, It was found that morphine. a-CAO, DADLE, MENK were able to inhibit the capacity of murine spleen cells to generate antibody and leukotriene C4 and conversely. dynorphin was able to stimulate the capacity of murine spleen cells to generate antibody and leukotriene C4. Morphine, a-CAO, MENK, DADLE, dynorphin decreased intracellular cAMP level, increased [Ca（2+）]i and calmodulin activity. The effects were completely blocked by naloxone, the specific opioid antagonist. Our results showed that opioids regulate the production of antibody in murine spleen cells, and alter intracellular cAMP, [Ca（2+）]i calmodulin activity. and leukotriene C4 production by way of binding to different receptor types.4|6|0Updated：2024-04-10
- Abstract：ＡＳｔｕｄｙｏｆｔｈｅＢｅｓｔＰｒｏｂｅｓｔｏＤｅｔｅｃｔＨＬＡ－ＤＰＢ１，ＤＱＢ１ＡｌｌｅｌｅＧｅｎｅｓＫｅＢｉｎｇｓｈｅｎ（克丙申）ａｎｄＳｕｎＸｉａｏｍｉｎｇ（孙晓明）(ＤｅｐａｒｔｍｅｎｔｏｆＩｍｍｕｎｏｌｏｇｙ，ＪｉｎａｎＭｉｌｉｔａｒｙＧ...4|8|0Updated：2024-04-10
- Abstract：Five restriction endonucleases were used to digest genomic DNA from 5 isolates of Trichinella spiralis obtained from Changchun, Tianjin, Xian, Henan and Yunnan. All the isolates were secured from pigs except the Changchun strain which came from dog. The DNA fragments digested by endonuclease were separated by agarose gel electrophoresis. The Changchun isolate had a EcoRI band at 1. 12kb and a DraI band at 1. 97kb which were unique to this isolate. A cloned specific repetitive DNA sequence （1. 12kb） from the Changchun strain was selected to prepare a probe for the Southern blotting of EcoRI restriction DNA fragments for the 5 isolates. The 1.12kb hybridizing band did not appear except in the Changchun isolate.These results seem to indicate that there are differences between the isolates obtained from hosts in different geographical regions.4|14|0Updated：2024-04-10
- Abstract：ＰｒｅｌｉｍｉｎａｒｙＡｎａｌｙｓｉｓｏｆｔｈｅＧｌｙｃｏｐｒｏｔｅｉｎｓｗｉｔｈＮ－ｇｌｙｃｏｓｙｌａｔｉｏｎｂｙＰｈａｓｅｏｌｕｓＶａｌｇａｒｉｓＡｇｇｌｕｔｉｎｉｎ（ＰＨＡ－Ｅ）ｉｎＣｈｉｎｅｓｅＰａｔｉｅｎｔｗｉｔｈＧａｓｔｒｏｉｎｔｅｓｔｉ...4|11|0Updated：2024-04-10
- Abstract：It has recently been shown that chimeric toxin composed of IL2 fused tp PE40, a mutant form of Pseudomonas Exotoxin A devoid of its native cell recognition and binding domain was cytotoxic to IL-2 receptor bearing cells. We here amplified the gene IL-2（60）, which cedes for the N-terminal 1-60 amino acids of human IL-2 by PCR. After that, we fused it to PE40 and the new chimeric protein IL-2（60）-PE40 was expressed in E. coli. SDS-PAGE revealed that IL-2（60）-PE40 chimeric protein accounts for more than 18% of total cell proteins. As the region IL-2 binds with its receptor was defined in the N-terminal residues 8-54 of IL-2, such fusion proteins will have the same activity with IL-2-PE40. Following primany purification, IL-2 （60）-PE40 was shown to be very toxic to IL-2 receptor-positive cells but non measurable effect on the cells lacking IL-2 receptors. Such a structure has not been reported by now. The fusion protein is useful for suppressing the immune response in cases of rejection of allografts and organ transplants and as therapeutic agents for the treatment of IL-2 receptor related diseases scuh as autoimmune disease, ATL（adult T-cell leukemia）, et al.4|8|0Updated：2024-04-10
- Abstract：Hyperpermeability is a crux of pathogenesis of sudden lung edema in many pulmonary disorders. especially in acute lung injury and adult respiratory distress syndrome（ARDS）. Using our modified method for assessment of pulmonary vascular permeability. we observed the effects of xanthine with xanthine oxidase（X-XO） perfused in rat pulmonary artery and the protection of vasoactive intestinal polypeptide（VIP） against the injury of pulmonary vascular permeabilrty. After addition of xanthine oxidase in the perfusate reservoir containing xanthine￣（125） I-albumin leak index （￣（125）IALI）was remarkably increased while peak airway pressure（Paw） was not significantly increased, and perfusion pressure of pulmonary artery（Ppa）and lung wet/dry weight ratio（W/D） were only slightly increased. Xanthine plus xanthine oxidase also increased thromboxane B2（TX B2） and 6-keto-prostaglandin F1α(6-keto -PGF1α) in the perfusate. Treatment with VIP obviously reduced or totally prevented all signs of injury. Simultaneously, VIP also diminished or abolished the associated generation of arachidonate products. The results indicated that VIP has potent protective activity against injury of pulmonary vascular permeability and may be a physiological modulator of inflammatory damage to vascular endothelium associated with toxic oxygen metacolites.4|8|1Updated：2024-04-10
- Abstract：Using mice infected with coxsackie B-3 virus （CVB3） as a viral myocarditis model, we observed the inhibitory effect of Astragalus membranaceus （AM） on CVB3-RNA replication in myocardial tissue of mice by RNA-RNA in situ hybridization with negative-strand RNA Probes labelled with ￣（35）S and quantitative imaging analysis of positive signals. The mechanism of its effect on CVB3-RNA replication has been investigated by detection of beta-interferon （β-IFN） as well. Results showed that the copy numbers of CVB3-RNA as well as the histologic scores （necrosis） in myocardial tissues of infected-AM treated mice were significantly lower than those in infected and normal saline treated mice, suggesting that AM could inhibit the replication of CVB3-RVA,but its effect on CVB3-RNA replication had no correlation with induction of β-IFN.4|13|21Updated：2024-04-10
- Abstract：ＴｈｅＣｌｉｎｉｃａｌＳｉｇｎｉｆｉｃａｎｃｅｏｆＭｅｔａｂｏｌｉｃＨｙｐｅｒｔｅｎｓｉｏｎＣｈｅｎＨｕｉ（陈慧）；ＣｈｅｎＭｅｎｇｙｉｎｇ（陈孟扬）；ｅｔａｌ．(ＦｕｊｉａｎＰｒｏｖｉｎｃｉａｌＲｅｓｅａｒｃｈＩｎｓｔｉｔｕｔｅｆｏｒＣａｒｄｉｏｖ...4|7|0Updated：2024-04-10
- Abstract：Coronary blood flow reserve （CFR） was assessed by transesophageal Doppler echocardiography in normal subjects （group A. n=20）,hypertensive non-left ventricular hypertrophy （non-LVH）Patients （group B,n=22）. hypertensive patients with LVH（group C.n=32）and coronary heart disease patients （group D. n=33） with the volume sample placed at the bifurcation of the left main and left main and left descending coronary artery. Coronary blood flow velocity （CBFV）was evaluated at rest.2 minutes after dipyridamole （0. 56mg/kg. i. v.） . and 2 minutes after aminophylline i.v. The ratio of dipyridamole to rest maximal diastolic velocity （D/R PDV） was considered the index of coronary blood flow reserve.It was found that D/R PDV was significantly less in groups C and D compared with that in groups A and B （D PDC,1.84±0. 57. 1. 57±0. 41 versus 2.59±0.70 and 2.22+0.58,respectively）,with no difference in D/R PDV between groups C and D.Twenty-four out of 32 patients in group C with D/R PDV were less than 2.0 compared to 29 out of 33 patients in group D （P>0.05）.Significant negative correlation was found between D/R PDV. D/R PSV and interseptal thickness. left ventricular mass index in hypertensive patients.These data show that impaired CFR in hypertensive patients with LVH is comparable to that in patients with coronary heart disease.4|8|0Updated：2024-04-10
- Abstract：Clinical, electrocardiographic and echocardiographic findings in 64 patients with dilated cardiomyopathy were retrospectively studied. Compared with 51 patients without complete left bundle branch block （CLBBB）, 13 patients with CLBBB had higher New York Heart Association （NYHA） functional class （P<0. 05）, increased left ventricular end-diastolic and end-systolic diameters （P<0. 002） and myocardial mass （P<0. 02）. severe mitral regurgitation （P<0. 01） and higher mortality rate （P<0. 04）. Multivariate stepwise regression analysis revealed that the presence of CLBBB was an independent prognostic factor for patients with dilated cardiomyopathy.4|7|5Updated：2024-04-10
- Abstract：Radioimmunoassays were used to measure the concentration changes of plasma endothelin（ET） and atrial natriuretic peptide（ANP） during the onset and after termination of paroxysmal supraventricular tachycardia（SVT）. 30 cases were reviewed and compansons with 42 normal subjects were made. There are very significant differences（P<0.0001） in the concentration changes of both plasma ET and ANP during the onset and 30 minutes after the termination of SVT. During the onset period of SVT. the plasma ET and ANP were markedly elevated and 30 minutes after its termination they were lowered significantly, but their concentrations were still 2-fold higher than ihose of the control group. As the biological effects of ANP and ET are antagonistic to each other. their parallel elevation and lowering of plasma concentrations during and.after the termination of SVT reveal that these 2 hormones parucipate in the pathophysiological process of SVT. This phenomenon is possibly one of the homeostatic regulatory functions in the organism.4|11|1Updated：2024-04-10
- Abstract：ＳｙｎｔｈｅｓｉｓａｎｄＲａｄｉｏｐｒｏｔｅｃｔｉｖｅＥｆｆｅｃｔｓｏｆＯｒｇａｎｉｃＳａｌｔｓｏｆ２，２－Ｄｉｍｅｔｈｙｌ－ＴｈｉａｚｏｌｉｄｉｎｅａｎｄＣｙｓｔａｍｉｎｅＷａｎｇＲｏｎｇｘｉａｎ（王荣先）；ＺｈｕａｎｇＸｉａｎｇｌｉａｎ（庄湘莲）...4|10|0Updated：2024-04-10
- Abstract：Immune-deficient nude mice were inoculated with nude mouse derived Mycobacteriurn leprae by multiple routes （intravenously. subcutaneously at the foot pads and ears）. The results showed that these inoculated animals were capable of producing a great number of Mycobacterium leprae to a level 10（11-12） per gram of tissue. and were detected histopathologically to have heavy leproniatous lesions. The dissemination of the infection was found particularly in sites with lower body temperature.The organisms have a partiality to striated muscles and peripheral nerves. The authors suggest that experimental leprosy in nude mice is a very useful tool for leprosy resarch. especially in countries without armadillos. Compared with the single-route inoculation reported previously. multiple-route inoculation is more available.4|4|0Updated：2024-04-10
- Abstract：In order to evaluate the accuracy of preoperative segmental localization of hepatic mass lesions,68 cases were prospectively studied and evaluated by the correlation of magnetic resonance imaging （MRI） and surgical findings. Right, middle and left hepatic veins were seen in 100%, 97% and 94% of the subjects respectively on MRI. The right and left portal veins were seen in 100% and 95.6% respectively. The accuracy in determining the segmental location of hepatic lesions was 89.7%. The accuracy could be increased to 91. 3% if the lesion was located at single segment whereas it was only 86. 4% when the lesion extended across several segments. It is possible to say that MRI is an important modality in the preoperative segmental localization of masses.4|8|1Updated：2024-04-10
- Abstract：ＡＣｌｉｎｉｃａｌＡｎａｌｙｓｉｓｏｎｔｈｅＴｈｅｒａｐｅａｔｉｃＥｆｆｅｃｔｓｏｆ３６０ＣａｓｅｓＡｃｕｔｅＨｉｇｈＡｌｔｉｔｕｄｅＰｕｌｍｏｎａｒｙＥｄｅｍａＴｒｅａｔｅｄｏｎｔｈｅＳｐｏｔｉｎＨｉｇｈＡｌｔｉｔｕｄｅＲｅｇｉｏｎｓＹａｎｇＪｉｎ...4|4|1Updated：2024-04-10
- Abstract：In a newborn rat model of sepsis, the changes of nitric oxide and the protective effects of methylene blue orland dexamethason were investigated. The results revealed that plasma nitric oxide levels were elevated at 6 h and peaked at 12 h after bacterial challenge. The treatment with methylene or/and dexamethasone was found to blunt hypoglycemia and hyperlacticemia, to reduce the occurrence rate of loss of response to pain, and to prolong the survival time. Moreover, therapy by dexamethasone was shown to decrease the 24 h mortality. The results suggested that nitric oxide play an important role during the course of fatal P. aeruginosa sepsis, but it is clear that the clinical value of nitric oxide and its inhibitors need to be further studied.4|4|0Updated：2024-04-10
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ESTABLISHMENT OF A HUMAN T-LYMPHOMA CELL LINE（H-TL90） AND ANALYSIS OF ITS BIOLOGICAL CHARACTERISTICS
Abstract：We established a human T-lymphoma cell line from the cancerous ascrtes of a male patient with prostate cancer which was named H-TL90. This cell line was characterized by its histological features, and by chromosomal and immunological analysis. Immunophenotypic analysis revealed that the cells expressed surface antigen CD3- CD4- CD7+ CD8-. Biological analysis revealed that the cell can promote lymphoryte proliferation. This suggested that the cell line has an autosecretion function. Cytogenetic analysis revealed that H-TL90 was a hyperdiploid with 47 chromosomes and had characteristic transloration between chromosome 3 and 11, and the deletion of the long arm of chromosome 6. These resuhs demonstrated the H-TL90 cell line can be a useful model for the study of human T-lymphoma.4|13|0Updated：2024-04-10 - Abstract：The effects of propofol and midazolam as an intravenous anesthetic were compared in 40 ASA Ⅰ-Ⅱ patients undergoing gynecological surgery during total intravenous anesthesia （TIVA）. They were divided into propofol group （Pn= 20） and midazolam group （Mn= 20） randomly. The anesthesia was designed for each group respectively. Here, we discuss the experimental method and the results, which indicate that propofol is not only an effective anesthetic but also has more rapid and head-clear recovery properties than midazolam.4|9|0Updated：2024-04-10
- Abstract：ＧｕｔＦｌｏｒａＤｉｓｏｒｄｅｒａｎｄＭｕｌｔｉｐｌｅＯｒｇａｎＩｎｊｕｒｉｅｓＣａｕｓｅｄｂｙＨｉｇｈＥｎｅｒｇｙＷｏｕｎｄｓＦｕＸｉａｏｂｉｎｇ（付小兵），ＷａｎｇＹａｐｉｎｇ（王亚平），ＹａｏＹｏｎｇｍｉｎｇ（姚咏明）ａｎｄＴｉａｎＨｕｉｍｉｎ...4|9|0Updated：2024-04-10
- Abstract：Tumor-infiltrating lymphocytes （TIL）isolated from metastatic lymph nodes in patients with nonoperable advanced gastric cancer were induced to become LAK-like cytotoxic activrty of TIL after in vitro culture with rlL-2.Twenty-three patients with advanced gastric cancer were treated by intravenously transfer of autologous TIL combined with rlL-2. The tumor forus disappeared （complete remission, CR） in 3 patients （13. 0%） and significantly decreased （partial remission, PR） in 5 patients （21. 7%）. Fifteen patients did not respond to the treatment. The amount of soluable IL-2 receptor in serum was significantly decreased after treatment, the cytotoxicity of NK cells and OT test were significantly increased. No significant difference in CD4/CD8 was found between before and after treatment. No serious side effect was obseved in the treatment.4|17|1Updated：2024-04-10
- Abstract：Twenty-eight cases of abdominal scar endometriosis from Janurary, 1980 to December, 1993 are reported. Of these patients, twenty-four underwent term cesarean section, and four underwent a midtrimester abortion by abdominal hysterotomy, The majority of patients manifested symptoms 1 year after the operation. The most common was a painful mass of scar tissue that became swollen and tender during menstruation. The pathogenesis, diagnosis and treatment are discussed. In correlation with the pathological findings, the effects of drug therapy are evaluated. It was found that surgical excision is the best method of treatment.4|15|1Updated：2024-04-10
- Abstract：ＳｔｕｄｙｏｆＤＮＡＲｅｐａｉｒＣａｐａｃｉｔｙｏｆＰｅｒｉｐｈｅｒａｌＢｌｏｏｄＬｙｍｐｈｏｃｙｔｅｓＦｒｏｍＡｇｅｄＰａｔｉｅｎｔｓｗｉｔｈＤｉａｂｅｔｅｓＭｅｌｌｉｔｕｓＧｅＷｅｉ（葛炜），ＣｈｅｎｇＹｉｎｍｅｉ（程吟梅）ＹｉｎＹａｐｉｎｇ(尹...4|6|0Updated：2024-04-10
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