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Volume ， Issue 1 ， 1996
- Abstract：The present study was designed to make certain whether there exists adrenomedullin （ADM） in the rat central nervous system and evaluated the hemodynamic actions of intracerebroventricular administration （ICVA） of human ADM[13-52]. By immunohistochemistry （ABC method）,We found that there was a discrete localization of ADM-positive immunoreactivity in the rat central system including cerebral cortex,paraventricular tissues, hypothalamus, cerebella cortex, mesencephalon and medulla oblongata. By reverse transcription-polymerase chain reaction（RT-PCR） analysis, rat ADM mRNA was found to be expressed in rat brain. These above results of immunohistochemistry and RT-PCR suggest that ADM exists in the rat brain. We also found that centrally administered ADM[13-52]in a dose of 0.4 to 3. 2 nmol/kg provoked marked, prolonged and dosedependent increases in mean arterial blood pressure （MABP） and heart rate （HR）. To clarify the mechanisms of the hemodynamic changes induced by centrally administered ADM [13-52]. the effect of centrally administered ADM [13-52] on renal sympathetic nerve activity （RSNA） was studied. The result showed that centrally administered ADM [13-52] （ 1. 6 nmol/kg） provoked a marked increase in RSNA .therefore .the increases in MABP and HR induced by centrally administered ADM [13-52]might be due to the stimulation of central sympathetic mechanism. In addtion,we also compared the relationship of activity and structure among the different fragments of ADM. In conclusion, ADMexists in the rat brain, and it may play an important role in the central control of cardiovascular system.4|12|0Updated：2024-04-10
- Abstract：ＥｆｆｅｃｔｓｏｆＨｙｐｅｒｔｏｎｉｃＳａｌｉｎｅＩｎｆｕｓｉｏｎｏｎＥｎｄｏｃｒｉｎｅＦｕｎｃｔｉｏｎｉｎＲａｂｂｉｔｓｗｉｔｈＨｅｍｏｒｒｈａｇｉｃＳｈｏｃｋｕｎｄｅｒＡｃｕｔｅＨｙｐｏｘｉａＺｈｏｕＱｉｑｕａｎ周其全ａｎｄＺｈｉＤｏｎｇｘｕｅ支...4|6|0Updated：2024-04-10
- Abstract：patients with angina decubitus （AD） were studied during hospitalization. These patients were found to have severe coronary artery obstructive lesions and an increase of myocardial oxygen consumption （MOC） before the onset to AD, indicating that AD belongs to the category of effort angina.18 patients were investigated by continuous hemodynamic monitoring. Three patients had significant increase in pulmonary artery diastolic pressure （PADP） before the onset. In the other 15 patients. PADP increased slightly in 12 and remained unchanged in 3 cases before the onset. Left ventriculography showed ejection fraction （EF）>45% in 25 of the 27 patients. These results indicate that left ventricular （LV） systolic dysfunction is not a major factor in the Pathogenesis of AD. The patients with LVEDP>12 mmHg constituted 60% of 25 patients with EF>45%, suggesting that these patients had ohvious LV diastolic dysfunction, which may be the major factor in the pathogenesis of AD.According to the results of our treatment, beta blockers may be used as the major form of tteatment in the patients with AD.4|13|0Updated：2024-04-10
- Abstract：ＡｓｐｉｒｉｎＰｌｕｓＣＥｆｆｅｒｖｅｓｃｅｎｔＴａｂｌｅｔｓ：ＡＰｒｏｄｕｃｔＯｆＮｅｗＴｅｃｈｎｏｌｏｇｙＡｓｐｉｒｉｎａｎｄＶｉｔａｍｉｎＣｃａｎｐｒｏｖｉｄｅｆａｓｔａｎｄｅｆｆｅｃｔｉｖｅｒｅｌｉｅｆｆｒｏｍａｖａｒｉｅｔｙｏｆａｉｌｍｅｎ...4|13|0Updated：2024-04-10
- Abstract：The recombinant PSG5-RARβ plasmid and the G418-resistant PSV2neo Plasmid （ 10 : 1） were cotransfected into PGCL3 cells by copreciprtation with calcium phosphate. The transfectants CR3 and CR4, which expressed the RARβ gene. were identified by Northern blot hybridization. The results showed that the in vitro growth and invasion of CR3 and CR4 were dramatically reduced compared to the control-transfected cell （CSV1）. Furthermore, the colony-forming abilities in soft agar and the tumorigenicrty in nude mice of CR3 and CR4 were abrogated. Our results suggests that RARβ functions not only as a receptor mediating the RA action, but also as a suppressor in lung tumorigenesis.4|16|0Updated：2024-04-10
- Abstract：ＦａｃｔｏｒｓＩｎｆｌｕｅｎｃｉｎｇｔｈｅＰｏｓｔｏｐｅｒａｔｉｖｅＳｕｒｖｉｖａｌＴｉｍｅｏｆＰａｔｉｅｎｔｓｗｉｔｈＢｒａｉｎＧｌｉｏｂｌａｓｔｏｍａＳｈｉＸｉａｎｇｅｎ石祥恩；ＷａｎｇＺｈｏｎｇｃｈｅｎｇ王忠诚ａｎｄＹａｏＢａｏｊｉｎ姚宝金(Ｄ...4|16|0Updated：2024-04-10
- Abstract：P-glycoprotein plays an important role in highly drug resistant cells. But its high expression cannot be acheived by chemotherapy. In order to study the effect of P-glycoprotein on clinical tumors, we established a low ADM resistant colon cancer cell line HR/ADM and determined the amplification and expression of mdr-1 gene. The GLC/ADM showed a resistant pattern similar to classical MDR and the transcription of mdr-1 gene determined by RT-PCR increased. The immunocytochemical analysis showed strong positive staining with monoclonal antibody. The gene amplification of mdr-1 was clearly demonstrated by southernblot. Our results suggested that moderate expression of P-glycoprotein might be enough for a high resistantpattern.4|6|1Updated：2024-04-10
- Abstract：The object of this study is to investigate the effect of VIP on pulmonary artery of chronically hypoxic rats. It was shown that chronic hypoxia depressed significantly pulmonary artery relaxation induced by VIP as compared with those of control （P<0. 001）. The vascular relaxation of both groups was correlated with concentration of VIP. In addition, the relaxant effect of VIP on pulmonary arteries in rats was endothelium─independent, and was not prevented by indomethacin or nordihydroguaiaretic acid, but was abolished completely by methylene blue. These results suggest that the lower relaxation of pulmonary artery in rats might not be due to the endothelial injury caused by chronic hypoxia, and chronic hypoxia may inhibit directly the soluble guanylate cyclase in vascular smooth muscle cells invloved in synthesis of cGMP and thus reduced the sensitivity and reactivity of pulmonary artery to VIP.4|10|0Updated：2024-04-10
- Abstract：ＬｙｍｐｈｏｃｙｔｅＳｕｂｓｅｔｓａｎｄＨＬＡ－ＤＲＥｘｐｒｅｓｓｉｏｎｉｎＬａｂｉａｌＳａｌｉｖａｒｙＧｌａｎｄｉｎＳｊｏｇｒｅｎ＇ｓＳｙｎｄｒｏｍｅＬｉＸｉａｏｃｈｕｎ李小春ａｎｄＷｅｎＺｈｕｘｉａｎ文竹咸(ＤｅｐａｒｔｍｅｎｔｏｆＳｔｏｍａｔｏ...4|10|0Updated：2024-04-10
- Abstract：Progressive inflammation and fibrosis are the central processes in the pathogenesis of pulmonary fibrosis. It is believed that macrophages in areas of chronically inflamed lung play a key role in fibrotic response. Therefore, we investigated the effects of alveolar macrophage （AmΦ） conditioned media from interstitial lung disease （ILD） patients on lung fibroblast proliferation and procollagen mRNA expression. After stimulating with AmΦ conditioned media from ILD patients, the fibroblast proliferation increased 71. 4 % compared with the control, but for media from bronchial carcinoma （BC） patients, it just increased 14. 3%. There is a significant difference between the two groups （P<0. 05）. The procollagen a1 （Ⅰ） mRNA in fibroblasts stimulated with AmΦ conditioned media from ILD patients was increased 21. 3 %, and a1 （ Ⅲ） was 37. 2% higher than control （P<0.05）. It increased 6. 8% and 12. 8% for media from BC patients respectively, but there was no difference when compared to the control. We considered that AmΦ from ILD patients might be in an activated state and could release some growth factors to stimulate fibroblast proliferation and promote collagen DNA expression.4|9|0Updated：2024-04-10
- Abstract：Hypoxia and endothelin-1 （ET-1） are associated with constriction of pulmonary vasculature both in vivo and in vitro. However, the role of hypoxia and ET-1 in the vascular remodelling during the development of pulmonary hypertension is unclear. This study demonstrated that ET-1（0. 1 nmol/L to 100 nmol/L） increased the [￣3H]thymidine uptake in a dose-dependent manner in cultured bovine pulmonary artery smooth muscle cells（PASMC）. which was enhanced by exposing PASMC to hypoxia （2%O2, 93%N2,5%CO2 ）. BQ123, the specific antagonist of endothelin receptor subtype A,eliminated the ET-1 medicated proliferation of PASMC and the cooperative effect of hypoxia. Some dilatory drugs could inhibite the mitogenic effect of ET-1. We also observed that hypoxia significantly increased [￣3H] thymidine uptake in PASMC without ET-1 and BQ123 could inhibite this effect. Radioimmunoassay suggested that there was an autocrine of ET-1 in cultured PASMC which was enhanced by hypoxia significantly.4|7|4Updated：2024-04-10
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INTRAPANCREATIC CHOLECYSTOKININ MEDIATES VAGALLY STIMULATED EXOCRINE SECRETION FROM THE RAT PANCREAS
Abstract：Although cholecystokinin is localized within neuronal fibres of the pancreas, a physiological role for intrapancreatic cholecystokinin has not been identified. The strategy of this study was to elicit pure vagal stimulation electrically, and to use specific receptor antagonists to identify the mediators of exocrine pancreatic secretion. We conclude that vagal stimulation of the rat pancreas involves ganglionic neurotransmission and release of acetylcholine and cholecystokinin from intrapancreatic, postganglionic fibres. To our knowledge, this is the first study to demonstrate a physiological role for intrapancreatic cholecystokinin.4|4|0Updated：2024-04-10 - Abstract：Glycoproteins from tumor cells isolated by Con A, PHAE and RCAI, were coupled to horse radish peroxidase and then used as reporters to evaluate the oligosaccharides change on serum glycoproteins in digestive tract diseases and individual healthy persons. A value 2 standard deviation above the mean of the healthy person group was considered positive. The results indicated that the positive rates of the oligosaccharides bound with Con A, PHA-E and RCAI were 51 % （61/119） . 70. 6% （84/119） , and 76. 4% （91/119） in patients with gastric cancer: 53. 3% （16/30）,46. 7% （ 14/30） and 66. 7% （20/30） in esophageal cancers; 28. 5% （15/49）, 49. 0% （24/49） and .46. 9% （23/49） in colorectal cancer; 78. 1% （25/ 32） ,81. 3% （26/32） and 31. 3% （10/32） in gallbladder tumors; 60. 4% （29/48）, 54. 1% （26/48） and 39. 5% （19/48） in hepatocellular cancer. The positive rates among the patients with benign diseases were also found to be 12. 7% （16/126）, 15. 5% （20/126） and 26. 2% （33/126）. However, the expression of the oligosaccharide moieties recognized by WGA on the glycoprotein bound with Con A, was significantly lower in the serum of patients with tumors than in those with benign disease and in healthy individuals （P<0. 01 , P<0. 01）.4|11|2Updated：2024-04-10
- Abstract：One hundred and twenty-four patients with heart disease were examined by omniplane TEE in order to systematically research every views of omniplane TEE, and further explore anatomy and image feature of each view. The result showed that omniplane TEE transducer can be rotated in probe from 0°to 180°.obtain many views at various angles behind the heart and fully demonstrate the structure and pathology of the heart and great vessels. It was useful for clinical diagnosis because of getting more information about the heart and great vessels. As omniplane TEE probe was little rotated in esophagus, it lessened esophagus stimulation. Meanwhile, it was suitable for three-dimensional reconstruction of left ventriculum.4|6|0Updated：2024-04-10
- Abstract：ＥｆｆｅｃｔｓｏｆＴｒｉｐｃｈｌｏｒｏｌｉｄｅ（Ｔ４）ｏｆＴｒｉｐｔｅｒｙｇｉｕｍＷｉｌｆｏｒｄｉｉＨｏｏｋｏｎｔｈｅＰｒｏｄｕｃｔｉｏｎｏｆＩｎｔｅｒｌｅｕｋｉｎ－６ｂｙｐｅｒｉｐｈｅｒａｌＢｌｏｏｄＭｏｎｏｎｅｕｃｌｅａｒＣｅｌｌｓａｎｄＳｙｎｏ...4|14|0Updated：2024-04-10
- Abstract：This paper presented the effects of systemic fluconazole therapy via intravenous （IV） and oral （PO） administrations on the adhesion of Candida albicans （C. albicans） to the buccal epithelial cells （BEC） from five treated patients with three candidosis, one mucormycosis and one sporotrichosis and at the same time.an analysis of the cell surface proteins involving candidal adherent receptor in the BEC of the patients in the course of 7 days were exposed to ￣3H-leucine radiolabeled C. albicans for in vitro candidal adherent assay.and the BEC from first intake day and the last intake day of the patients were extracted by dithiothreitol （DTT）-iodoacetamide treatment for SDSPAGE. These results indicate that the systemic fluconazole therapy results in the inhibitory effect of candidal adhesion to BEC of treated patients to prevent them from oral candidosis for a prolonged time, which is based on the absent surface protein （35 KDa） of the BEC.4|8|0Updated：2024-04-10
- Abstract：Washed human platelets were loaded with the Ca2+-sensitive photoprotein, aequorin. using hypoosmotic shock treatment-technique. Then aggregation and cytoplasmic ionized calcium concentration （ [Ca2+] i） changes in response to collagen or thrombin were measured simultaneously in the aequorin-loaded human platelets with a Platelet Ionized Calcium Aggregometer. 764-3. an active component isolated from the Chinese medicinal herb Salvia Miltiorrhiza Bge, inhibited platelet [Ca2+]i rise as well as aggregation evoked by collagen or thrombin in the presence of extracellular Ca2+. After the extracellular Ca2+. was removed by addition of EGTA, collagen or thrombin. causing no aggregation. still elicited platelet [Ca2+] i rise which reflected Ca2+ mobilization from intraplatelet stores. Under this condition, 764-3 could also suppress platelet [Ca2+] i rise. Analysis shows that 764-3 inhibrts platelet Ca2+ influx and Ca2+ mobilization with similar potency. which accounts for its suppression of platelet [Ca2+] i rise, and must contribute to its inhibition of platelet aggregation.4|24|3Updated：2024-04-10
- Abstract：This study differentiated pseudocondyloma of vulva from condyloma acuminata using dot blot hybridization and polymerase chain reaction （PCR）. A total of 27 cases of pseudocondyloma of vulva and 65 cases of condyloma acuminata were selected for the study. The genital lesions were examined clinically and were biopsied. Each biopsy was subjected to histological examination and HPV DNA analysis by dot blot hybridization and PCR. Dot blot analysis detected HPV DNA in 19（82. 6%） out of 23 cases of condyloma acuminata and 2（25% ） out of 8 cases pseudocondyloma of vulvae （P<0. 05）. PCR detected HPV DNA in 51 （92. 7%） out of 55 cases of condyloma acuminata , compared with none in 23 cases of pseudocondyloma （P<0.001 ）. HPV DNA was present in the majority of condyloma acuminata specimens. HPV 6 and 11 were the predominant types. Peudocondyloma is probably not associated with HPV. PCR was the most sensitive and useful technique for HPV DNA detection.4|8|0Updated：2024-04-10
- Abstract：ＣｕｔａｎｅｏｕｓＳｐｏｒｏｔｒｉｃｈｏｓｉｓＴｒｅａｔｅｄｗｉｔｈＴｅｒｂｉｎａｆｉｎｅＷｕＳｈａｏｘｉ吴绍熙；ＧｕｏＮｉｎｇｒｕ郭宁如ａｎｄＬｕＧｕｉｘｉａ吕桂霞(ＩｎｓｔｉｔｕｔｅｏｆＤｅｒｍａｔｏｌｏｇｙ，ＣＡＭＳ＆ＰＵＭＣ，Ｎａｎｊｉｎｇ２...4|6|0Updated：2024-04-10
- Abstract：The incidence of sexually transmitted diseases（STDs） increased from 26. 04 per 100 000 in 1987 to 104. 81 per 100 000 in 1993 in selected areas of the country. Gonorrhea is by far the most common STD but its constituent ratio declined because of a rapid increase of nongonococcal uretheritis and genital warts during most recent years. The incidence of syphilis is relatively low and cases of congenital infection are noted. The wide spread of resistant Neisseria gonorrhoeae infection gives a challenge to the therapeutical and control strategies of STDs. Sexually transmitted Chlamydia trachomatis infections, an important cause of urethritis. cervicitis and pelvic inflammatory disease. is becoming common in our country. Attention has been drawn on viral hepatitis in their means of transmission by sexually behaviors, and also, on the homosexuals, assumed to be the high risk group to catch STDs. Coordinated national efforts to control STDs in China have been taken.4|7|0Updated：2024-04-10
- Abstract：ＥｘｐｌｏｒａｔｉｏｎｏｆｔｈｅＳｐｅｃｉｆｉｃｉｔｙｏｆＮｅｏｎａｔｅＴＳＨ－ＩＲＭＡＭｅａｓｕｒｅｍｅｎｔｆｏｒＭｏｎｉｔｏｒｉｎｇＩＤＤＳｈｉＬｉｘｉｎ时立新；ＭａＱｉｌｉｎｇ马启玲；ＳｈｉＺｈｏｎｇｆｕ时钟孚；ＺｈａｎｇＪｉａｘｉｕ张家秀ａｎ...4|8|0Updated：2024-04-10
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