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Volume 0 ， Issue 1 ， 1997
- Abstract：The distribution of the immediate early gene c-fos expression in the mouse central nervous system after subcutaneous injection of Bay K 8644 was observed immunohistochemically. Half an hour after injection, cfos protein （FOS） was expressed in the piriform cortex, sensorimotor cortex, caudate putamen, thalamic paraventricular nucleus and striate cortex,etc. Intense FOS immunoreactive （FOS-ir） cells were seen during 2 ～ 4 h after injection. The restllts suggested that the distribution of FOS-ir ce1ls after subcutaneous injection of Bay K 8644 was coincldent with that of L-type calcium channels in the different areas of the CNS. After Bay K 8644 injection, mice appeared seizure-like behavior. The percentage of cells double-labelled by FOS and CaBP immunoreactivities in the observed regions was about 60. 2～72. 8% in CaBP-ir cells. It suggested that most CaBP-lr cells may have L-type calcium channels.Keywords：c-fos;Bay K 8644;immunohistochemistry4|13|0Updated：2024-04-10
- Abstract：Syrian hamster ernbryo（SHE） cells are irradiated by a particles emitted from Plutonium-238 in vtiro.Transfection frequencies of donor DNA derived from passaged cells,including monocloned cells from 20th passage cells,after irradiation are parallel with their tumorigenicity of donor cells. After trans formation, expressions of H-ras and c-myc genes are in the state of activation, which might be resulted from occurrence of mutation in H-ras gene and hypomethylation of c-myc gene respectively, even though no alteration was observed on their restriction fragment length polymorphisms（RELP） patterns of both genes. On the other hand,expression of p53 gene is also found to be in the state of activation in transformant, but no heavy staining of p53 protein appeared in the transformant-derived tumor with p53 specific McAb HD 200. This might be result from the partlal deletion of p53 cDNA in transformant by reverse transcriptional polymerase chain reaction（RT PCR）. These results imply that activation of H-ras and c-myc genes coupled with inactivation of p53 turnor suppressor gene play important role in a particle-induced cell trans formation.Keywords：a particles;cell transformation;oncogenes4|6|0Updated：2024-04-10
- Abstract：Breast cancer containing estrogen receptors （ER ） are responslve to antiestrogen treatment and have a better prognosis compared with ER-negative tumors. The loss of estrogen receptors appears to be associated with a progression to less clifferentiated cells. We transfected the human ER into the ER-negative breast cancer cell line MDA MD 231 cells. We found that expresslon of adequate ER is strong associated with the ability of human breast cancer cell growth inhibition and progression. Compared with nontransfected or mock-trans fected cells, ER-transfected cells exhibited growth slower, forming smaller colonles in soft agar and growth inhibited by estrogen and tamoxifen. Therefore reactivation or transfection of the estrogen receptor gene can be considered as therapeutic approaches to hormone-independent breast cancer.Keywords：estrogen receptor;breast cancer;stable transfection4|38|5Updated：2024-04-10
- Abstract：A pair of oligonucleotide primers were designed to amplify the endoflagella gene of L. Interrogans serovar lai. An approximately 840 bp fragment was generated with PCR and inserted into plasmid pUC8,after the fragment and pUC8 were digested respectively with BamHI and PstI. A recombinant plasmid （designated as pLF1 ） was obtained. SDS-PAGE analysis indicated that a 33 kDa was expressed in E. Coli JM103 harboring pLF1 and the expression level of the protein was 11 % of the total bacterial soluble proteins. Western blot analysis showed that the protein band could be recognized by the antiserum against the endoflegella （Axial filament ） of Leptospira interrt,gans serover lai. Nucleotide sequence data showed an open reading frame encoding 282 aminoacids residues,corresponding to a protein of molecular weight 33. 6kDa. Comparison of the deduced endoflagellar subunit protein （flaB） amino acid sequence with flagellins from other bacteria revealed a high level of identity with the Trehoema pallid um flaB proteins. Immunization/protection experiment was performed on the model of BALB/C mice and showed that there was higher survival rate in the group JM103-pLE1 than in the group JM103-pUC8.Keywords：leptospira;endoflagellin;sequencing4|11|0Updated：2024-04-10
- Abstract：We report novel mutations in exon 7 of human phenylalanine hydroxylase （PAH） gene of phenylketonuria （PKU ） in southern Chinese, analysed by using PCR-DGGE （denaturing gradient gel electrophoresis ）, solid phase DNA sequencing and Ih vliro expression. One of the 2 novel mutations, IVS6nt1, is an intron-exon Junctional mutation which results a splicing defect in mRNA. Arg252Gln is another novel mutation with residual PAH activity only 24 % compared to wild type in in vitro mutagenesis and expression in Cos-1 cell. Other 3 known mutations and polymorphism including Arg241Cys, Arg243Gln and Val245Val（GTG to GTA） together with these novel mutations composed the mutatlonal profile of exon 7 in the PAH gene of PKUs in this populations.Keywords：phenylketonuria;phenylalanine hydroxylase gene;mutation4|27|0Updated：2024-04-10
- Abstract：This study compares the persistence and histological characteristics of gut suture with those of human amnion collagen, bovine collagen, and Vicryl suture implants in rats. Gut suture and human amnion collagen more resembled living tissue than did bovine collagen and were characterized by their cel1ularity and the presence of numerous capillaries. The Vicryl suture implants were quickly absorbed. Picrosirius polarization revealed the synthesis of host collagen by rat fibroblasts which immigrated into the gut suture and human amnion collagen implants. The authors suggest the potential of gut suture as a soft tissue substitute to improve linear skin contour deficits.Keywords：biomaterial;collagen;gut suture4|8|0Updated：2024-04-10
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- Abstract：The safety and toxicity of chemicals given first to animals and finally to humans are generally estimated with a method of safe coefficient, which is scientifically a way lack of grounds. To make a change of the old method, we designed a Physiologically Based Pharmacokinetics Medel for the estimate of safety and toxicity of chemicais. As an example,p-nitrophenol sodium （PNP-Na） is used in the research work. Studies of the PNP-Na pharmacokinetics in bodies of rat as well as humans are made, and possibilities of making use of the Model in the estimate of safety and toxicity of chemicals are discussed.Keywords：physiologically based pharmacokinetics model （PBPK ）;p-nitrophenol sodium （PNP-Na）;estimation of safety of chemical4|27|1Updated：2024-04-10
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- Abstract：HBV Pre S1 sequence is supposed to play an important role in the infection of HBV. Presence of Pre S1 /anti-Pre S1 in serum has valuble clinical imphations. In order to improve the study of Pre S1, Pre S1 sequence was overexpressed in E. coli as a fusion protein with MBP （Maltose- binding Protein）, and anti-Pre S1 antiserum was elicited in rabbits by Pre S1 MBP purified by affinity chromatography. The recombinant plasmid constructed from PMAL-cRI expressed the 106aa Pre S1 sequence at the C terminal of MBP by tac promoter. The resulting Protein is about 54kD in size. Western-blot analysis confirmed its reactivity with antiserum derived from synthetic Pre S1 peptide and serum from patients with acute hepatitis B （AHB）. ELISA showed that Pre S1-MBP and Dane Particles purified from AHB patient’s serum reacted with antiserum against synthetic Pre S1 peptlde,and this reaction was specifically inhibited by synthetic Pre S1 peptide. ELISA also demonstrated that antiserum against Pre S1-MBP reacted with synthetic Pre S1 peptide, but not with synthetic HCV peptide or HEV peptide.Keywords：HBV;gene expression;E. coli4|19|1Updated：2024-04-10
- Abstract：This study was designed to systematically investigate expression of TNF α,IL-1β,I1-6 mRNA in the liver,lungs and kidneys, release of TNF α in the above tissues,their relationship with hepatic, pulmonary and renal dysfunction,and distribution of endogenous endotoxin in tissues after hemorrhagic shock in mice and rats, with reverse-transcription-polymerase chain reaction, ELISA, etc, to elucidate the kinetics of expression and release of major cytokines in vital organs,their role and mechanism of production in shock. The results were: ①expression of TNF α, IL-1 β, IL-6 mRNA in vital organs successively increased after hemorrhagic shock and resuscitation,and TNF α expression was the first to appear followed by IL-1β. Though expression of IL-6 mRNA appeared late,it persisted longer;②TNF α levels in the liver,lungs and kidneys were all elevated but to different degrees after shock and resuscitation. At 3 hours after resuscitation,TNF α levels in the three above tissues were still significantly high,while plasma TNF α levels were already decreased to control levels; ③hepatic,pulmonary and renal functions were damaged to different degrees after hemorrhagic shock,with hepatic dysfunction being the most severe; ④endotoxin levels in the liver, lungs and kidneys were markedly increased after shock and resuscitation,and paraIleled the expression of cytokine genes. In addition,there was slgniflcant correlatlon between changes in endotoxin level in tissues and TNF α release in tissues during early shock. lt is suggested that expression and release of cytokines in vital organs might play an important role in local organ damage after hemorrhagic shock,and production of cytokines is related to endotoxin translocation.Keywords：hemorrhagic shock;cytokines4|15|9Updated：2024-04-10
- Abstract：This study was done to define the human genital immune response to infection with Neisseria gonorrhoeae. The semen specimens were obtained from 15 patients with uncomplicated gonococcal infection in the acute and convalescent phases and 15 men with uninfected control- After precipitated with amoniasulfate,the semen was tested against the outer membrane protein of gonococcal isolates from the same patients to examine antigen-antibody interactions by use of the western blot technique. The antibodies in the semen reacted with more gonococcal antigens in the acute phase than in the convalescent phase. IgA in the semen reacted with more antigens than did IgG in the same specimens. The predominant reacted antigens were protein I, protein II, 46～ 48, kD, 14 ～16 kD and 88～ 90 kD protein.Keywords：Neisseria gonorrhoeae;outer membrane protein;antibody4|6|0Updated：2024-04-10
- Abstract：Objective.To study the speciality of orbicularis oculi muscles, nerves and oris muscles, nerves and mechanism of difference of faclal muscular injury in facial paralysis deeply.Methods. The conductive velocity of the efferent nerves of orbicularis oculi and oris muscles of the health human beings and guinea pigs is measured with electromyogram （EMG） apparatus.Results. The conductive velocity of orbicularis oculi nerve is quicker and oris muscles are controlled by facial nerves on both sides. Measuring the threshold of Strength-Duration （S-D） curves of the motor point of orbicularis oculi and oris muscles shows the threshold of the former is lower. Measuring the diameter of orbicularis oculi and oris nerve fibers on the guinea pigs and rabbits shows the diameter of orbicularis oculi nerve fiber is bigger. The area of secondary synapse space of orbicularis oculi motor end plate is larger than that of oris under scanning electromicroscope.Conclusions. Orbicularis oculi muscles,orbicularis oculi nerves and oris muscles,oris nerves all have their own characteristic on physiology and anatomy. It elucidated the mechanism that orbicularis oculi muscle is easy to be injuried.Keywords：facial nerve;action potentials;motor endplate4|14|2Updated：2024-04-10
- Abstract：Background. Decrease in intraocular pressure （IOP） during pregnancy has been reported by previous studies,but amount of decrease varies from study to study. Moreover,previous studies have concentrated on the last trimester of pregnancy or one reading per trimester. IOP changes throughout the pregnancy have never been described. Therefore,the present study was planned to determine IOP throughout the pregnan cy,after placing control on IOP affecting factors.Subjects and methods. Thirty-six pregnant and same numbers of non-pregnanat women of the same age group were studied. IOP measurements were taken at four week intervals throughout the pregnancy,with the Goldmann applanation tonometer.Results. As compared to control subjects,up to 8th week,IOP remained the same. At 12th week,it becomes significantly lower. The IOP differences between fIrst and second （P<0. 05）,and second and third （P<0. 001 ） trimesters of pregnancy were significant. In every subject, IOP decreased during pregnancy.However,there were variations among individuals in the extent of IOP decrease Conclusbos. With advancing pregnancy, intraocular pressure decreases. Knowledge of the normal level of intraocular pressure in various stages of pregnancy may help glaucoma screeners.Keywords：ocular pressure;pregnancy;tonometry4|7|2Updated：2024-04-10
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- Abstract：Uging diet control method to feed the weaning male Wistar rats with selenium and iodine deficiency Keshan endemic area food for 8 weeks to set up animal model. Singal channel recortling in cell attached model was used to measure cardiac cell membrane potassium channel conductances. which coincide with the cardiac cell membrane potassium channel conductances in normal Wistar rats.The potassium channel conductance on selenlum and lodine cleficiency rat cardiac cell membrane is showing current-voltage increasing lineally in the range of clamping volatge 0±30 mV with channel conductance of 43.4 pS. The channel current does not increase depending on the clamping voltage that is showing the rectifying characteristic and the channel current amplitude can be augmented by added KIOa 5 mmol/L in bath solution. A kind of inward rectiyfing potassium channel activity was recorded, but this channel activity disappeared affer lasting 6～ 10 minutes only. Then an inward rectifying potassium channel with the conductance of 11. 2 PS was activated by KIOa 5 mmol/L, introduced to bath solution. Both conductances are less than that of normal Wistar rats.Keywords：cardiomyocytes;selenium;iodine;potassium channel4|8|0Updated：2024-04-10
- Abstract：Objective. This study analyses the clincopathologic features and hormone receptor level of breast cancer in aged patients（≥60 years） and explores the related factors of the prognosis.Methnds. For all patients,the clinical data and pathologic data were reviewed retrospectively. Followup data were available. Of the 308 cases,tumor specimens in 130 patients were subjected to hormone receptor analysis by a dextran-coated charcoal technique.Results’ Of the 308 cases, 82 cases were in stage I, 147 cases in stage II, 66 cases in stage III,and 13 cases in stage IV. Metastatic rate （axillary lymph nedes） was 35. 7%. The overall postoperative 5 and 10-year survival rates were 70. 8 % and 50. 5 %,respectively. Estrogen receptor and progesterone receptor positive rates were 66. 9 % and 40.0%,respectively. The survival rate was decreased with the increasing grade of TNM（P<0.01）. The mortality rate of the breast cancer was related to the clinical stage and the hormone receptor level （P<0. 05 ）.Conclusbos. There was a relatively gdri prognosis in this series. The size of tumor, number of metastatic nedes and hormone receptor level were related to the prognosis.Keywords：breast cancer;estrogen;progesterone;prognosis4|7|0Updated：2024-04-10
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