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Volume ， Issue 3 ， 1999
- Abstract：Effects of genistein on invasion and matrix metalloproteinase activities were investigated in HT1080 human sarcoma cells.Invasion of HT1080 cells through reconstituted basement membrane was inhibited when the cells were treated with 100 μ mol/L and 200 μ mol/L genistein.At the same concentrations,genistein not only suppressed latent forms of matrix metalloprotinese 2 and 9（MMP 2 and MMP 9） to convert into active forms,but also increase dramatically the tissue inhibitor of metalloproteinase（TIMP 1） mRNA contents and reverse the imbalance of MMPs and TIMPs.However,expressions of MMP 2 and MMP 9 were not significantly affected.Suppression of MMP activation and increase of TIMP 1 expression will decrease matrix degradation by MMPs,and consequently inhibit invasions of the cells.These results emphasized the existence of the imbalance between MMPs and TIMPs in tumor invasion and metastasis formation.The value of genistein as a drug for antiinvasion and anti metastasis chemotherapy was suggested.4|14|0Updated：2024-04-10
- Abstract：Mutations of the p53 tumor suppressor gene are the most frequent genetic alterations detected in human lung cancer. To assess the pathogenic significance of p53 gene alterations in Chinese non small cell lung cancer（NSCLC）,74 paired samples of primary lung cancer and normal lung tissue far away from the cancer were analyzed for mutations of the p53 gene（exons 5 8） using exon specific PCR, single strand conformation polymorphism （PCR SSCP）. p53 mutations were observed in 55 4%（41/74） of the samples. No linkages were detected between the incidence of p53 mutations and histological type, lymph node metastasis,age or sex. Significant association between p53 mutations and degree of differentiation in adenocarcinomas, not in squamous cell carcinomas, was observed. The frequency of p53 mutations in smokers（65 3%） was higher than in nonsmokers（33 3%） and reached statistical significance.We also found p53 mutations in 6/7 samples which had tissue invasion and distant metastasis.These results suggest that smoking could be an important factor in lung carcinogenesis,p53 mutation is a worse prognosis indicator in adenocarcinomas and related to high aggressive behavior of human lung cancer.5|8|0Updated：2024-04-10
- Abstract：The monoterpene d limonene inhibit the plasma membrane associated P21 ras expression and the posttranslational isoprenylation of P21 ras , a mechanism that may contribute to its efficacy in the chemoprevention and therapy of chemically induced rodent cancers and some human solid tumor cells. In the present study,the relative abilities of d limonene to inhibit membrane associated P21 ras expression in pancreas tumor cell（PaCa） was carried out with Western blotting, and the inhibition of farnesyl protein transferase （FTPase） activity during the Ras protein isoprenylation and cell proliferation were determined.Concomitantly,the effects of d limonene on P21 ras localization by immunohistochemistry and H ras oncogene expression in PaCa tumor cell line by Northern blotting were observed. The results showed that d limonene inhibited FPTase activity, thus to reduce P21H ras isoprenylation. d limonene could decrease P21 ras membrane association and increase cytosolic accumulation of P21 ras . This phenomenon was also noted when d limonene treated PaCa cells were stained immunohistochemically with anti P21 ras antibody. It is suggested that the inhibition of FPTase activity was closely related with the inhibiton of P21 ras membrane association and the alteration of P21 ras localization. Inhibition of farnesylation of P21 ras altered their intracellular localization and, hence, disrupted their biological activity,but no relationship with H ras oncogene expression was found.4|10|1Updated：2024-04-10
- Abstract：The aim of this study was to compare six methods of detecting apoptosis induced by extracellular adenosine triphosphate （ATP） in human leukemic lymphocytes with purinergic P2Z receptors.These methods used were electron microscopy（EM）, detection of internucleosomal DNA fragmentation by agarose gel electrophoresis, autoradiographic analysis of DNA fragmentation, in situ labeling of DNA strand breaks with fluorescein dUTP and exogenous terminal deoxynucleotidyl transferase （TUNEL）, quantitation of 3 ends of DNA breaks by labeling with α 32 PdCTP（TdT assay）, and quantitation of apoptotic cells with fluorescein annexin V using flow cytometry（FCA）.We found EM and detection of DNA ladder pattern by agarose gel electrophoresis to be specific,but lacking in sensitivity. The combination of autoradiography and gel electrophoresis gave an increase in sensitivity of at least 50 fold although, of all the methods, the TdT assay was shown to be most sensitive. The four methods for quantifying apoptosis EM, FCA, TUNEL and TdT assay proved to be reliable and gave statistically similar results on apoptotic lymphocytes. These observations indicate it is essential to combine specific, sensitive and quantitative techniques in detecting apoptosis.4|8|2Updated：2024-04-10
- Abstract：Objective.In order to demonstrate the binding of HBV X protein （HBX） with the general transcription factor TFIIB. Methods.In vitro glutathion S transferase （GST） resin Pull Down assay and Far Western Blotting assay, in vivo Co immunoprecipition assay were used. Results.The X199（51 99） domain of HBX is reponsible for HBX binding to TFIIB. While the d10 domain （125 295） of TFIIB is required for TFIIB binding to HBX. When the two basic amino acids（K） at position 178 and 189 of TFIIB were substituted by neutral amino acids（L）, the binding of TFIIBK178L and K189L to HBX was siginificantly reduced. When the the basic amino acids were substituted by the acidic amino acids（E）,the binding of TFIIB K178E and K189E to HBX were almost lost. In vitro results of HBX binding to TFIIB were further confirmed by in vivo co immunoprecipitation assay. Our results also indicated that the Woodchuck hepatitis virus X protein （WHX） interacts with TFIIB. Conclusion.These results suggested that the communication between HBX and general transcription factor TFIIB is one of the mechanisms which account for its transcriptional transactivation.4|27|5Updated：2024-04-10
- Abstract：The activation of T cells to differentiate and to proliferate is an essential step in the immune response to antigen, especially in cell mediated acute allograft rejection. Besides the interaction of CD3/TCR complex with Ag/MHC complex presented on antigen presenting cells, a complete T cell activation and proliferation requires a second costimulatory signal. The interaction of CD28/CTLA 4 and B7 is a major costimulatory pathway for T Cell activation. Inhibition of this pathway results in development of antigen specific unresponsiveness and clonal anergy.In present study,the biologic function of anti CD28 monoclonal antibody and its Fab fragment were investigated in vitro and in vivo.The results indicate that mAbCD28 and its Fab fragments could promote the functional recovery of allografts and prolong the graft survival,but could not reverse the acute rejection or induce transplantation tolerance in the rat PTG allograft model. We also found that peripheral TNF α level and NK cell activity were suppressed in the presence of mAbCD28 and its Fab fragments for a relatively long time after PTG transplantation.4|11|2Updated：2024-04-10
- Abstract：ｔｉｓｑｕｉｔｅｃｏｍｍｏｎｆｏｒｌｉｖｅｒｃｉｒｈｏｓｉｓｔｏｂｅｆｏｌｏｗｅｄｓｕｂｓｅｑｕｅｎｔｌｙｂｙｋｉｄｎｅｙｉｍｐａｉｒｍｅｎｔ，ｗｈｉｃｈ，ｂｅｉｎｇｒｅｃｅｓｉｖｅｉｎｓｔａｔｅａｎｄｓｈｏｗｉｎｇｎｏｃｌｉｎｉｃａｌｓｙｍｐｔｏｍ...4|7|0Updated：2024-04-10
- Abstract：Objective.The purpose of this study was to assess the renal graft expression of ICAM 1（intercellular adhesion molecule 1） and LFA 1（lymphocyte function associated antigen 1）molecule with relation to graft rejection. Methods.Rat kidney transplantation was performed according to the procedure of Kamada with some modification.Experimental rats were divided into 5 groups.The survival time of recipient rats and function of grafts after renal transplantation were observed.The sections of renal graft were stained for monoclonal antibody ICAM 1 and LFA 1, and then quantification of ICAM 1 and LFA 1 expression was accomplished by computer image analysis. Results.ICAM 1 and LFA 1 increased significantly in the renal allograft rejection group as compared with the non rejection groups（P<0 05）. Conclusion.Both biopsy of renal graft and monitoring of ICAM 1 and LFA 1 are useful tools in diagnosing and treating acute rejection.4|30|2Updated：2024-04-10
- Abstract：The purpose of this study was to determine the efficacy of using an ultrasound contrast agent（levovist）to enhance the color Doppler imaging of liver neoplasms.Thirty patients with hepatic tumors were enrolled in this study.After intravenous administration of levovist,the color Doppler signals of normal hepatic vessels were enhanced.In various hepatic tumors,the different patterns of tumor vascularity were observed,which had not been demonstrated in conventional non contrast color Doppler imaging.In 11 of 16 patients with hepatocarcinoma,additional color Doppler signals were observed in the central part of the tumors.On the contrary,3 patients with metastatic liver lesions the enhanced color Doppler signals appear only at the peripheral of tumors.A typical rim like color enhancement was seen in 2 of the 3 cases.In six patients with hepatic hemangiomas contrast enhanced color Doppler imaging demonstrated the blood vessels at the margin of the neoplasms.Contrast enhanced color Doppler imaging improves the visualization of the hepatic neoplasm vascularity.This technique holds great promise for detecting small liver tumors and differentiating hepatic neoplasms.4|37|6Updated：2024-04-10
- Abstract：Objective.The purpose of this study was to determine the impact of intraoperative ultrasound（IOUS）on the management of patients with neoplasms of the liver. Methods.Forty nine patients operated on for liver or other pathologic processes were examined intraoperatively with 5.0 MHz special ultrasound transducers during surgical exploration of the abdomen.Subjects were evaluated because of known or suspected disease of the liver.Preoperative imaging studies included percutaneous ultrasound（n=49）,magnetic resonance imaging（n=11）,and computed tomography（n=34）.Intraoperative evaluation on all patients included inspection,bimanual palpation,and ultrasonography.Comparison between preoperative imagings and IOUS were analysed. Results.Sensitivity for detection of hepatic neoplasms showed in intraoperative ultrasound,percutaneous ultrasound,magnetic resonance imaging and computed tomography as 100%（23/23）,74%（17/23）,74%（14/19） and 75%（6/8）.Specificity showed 100%（26/26）,100%（26/26）,93%（14/15） and 67（2/3）.In seven patients（14%）,the neoplasms were not found by inspection,bimanual palpation,and identified only by IOUS. Conclusions.Intraoperative ultrasound is the most sensitive and specific method for detection and surgery of liver neoplasms,especially the occult neoplasms and small size lesion（<2cm）.4|13|0Updated：2024-04-10
- Abstract：Complications occur frequently after surgical treatment of scoliosis.In order to prevent from them effectively,101 cases with failure and postoperative complications were analysed.They included rod fracture in 22 cases（15 Harrington rods,4 Zielke rods,and 3 Luque rods）;recurrence of curve severity in 12 cases;broken or loossened luque wires in 15 cases;loss of thoracic kyphosis（flat back） in 6 cases;progressive kyphosis with or without paraplegia following incorrect posterior decompression in 5 cases;and increased unbalance of shoulders after instrumentation in 2 cases due to neglect of the tilting of the first thoracic vertebra.Infection occurred in 8 cases（incision infection 7 cases,deep wound infection in 1 case）;and pneumothorax in 1 case.They were induced by biomechanical factors in 23 cases（22 8%）,incorrect selections of indications in 29 cases（28 7%）,operational mistakes in 37 cases（36 6%）,internal fixation factors in 15 cases（14 9%）.The authors hold that there are quite a lot of factors leading to occurrence of complications and the effective way for prevention from them is to understand the factors and main technical points related to internal fixation.4|26|0Updated：2024-04-10
- Abstract：Ｅｎｄｏｇｅｎｏｕｓｆｏｒｍａｔｉｏｎｏｆｎｉｔｒｉｃｏｘｉｄｅ（ＮＯ）ｉｎｖａｒｉｏｕｓｔｉｓｕｅｓｐｌａｙｓａｎｉｍｐｏｒｔａｎｔｒｏｌｅｉｎｃｅｌｆｕｎｃｔｉｏｎａｎｄｃｅｌｃｏｍｍｕｎｉｃａｔｉｏｎｂｏｔｈｉｎｈｅａｌｔｈａｎｄｄｉｓｅａｓ...4|6|0Updated：2024-04-10
- Abstract：Objective. To testify the phenomenon that large amplitude action potential appears at the early stage of facial paralysis, and to search for the mechanism through clinical and experimental studies. Patients（animals） and methods. The action potentials of the orbicular ocular and oral muscles were recorded in 34 normal persons by electromyogram instruments. The normal range of amplitude percentage was found out according to the normal distribution. One hundred patients with facial paralysis were also studied. The action potentials of facial muscles were recorded in 17 guinea pigs before and after the facial nerve was compressed and the facial nerve was examined under electromicroscope before and after the compression. Results. The amplitude percentage of the suffered side to the healthy side was more than 153 percent in 6 of the 100 patients. Large amplitude action potential occured in 35 per cent guinea pigs which were performed the experiment of facial nerve compression. Electromicroscopic examination revealed separation of the lammae of the facial nerve’s myelin sheath in the guinea pigs which exhibited large amplitude action potential. Conclusion. The facial nerve exhibited a temporary over excitability at the early stage of facial nerve injury in some patients and guinea pigs. If the injury was limited in the myelin sheath, the prognosis was relatively good.4|20|0Updated：2024-04-10
- Abstract：ＴｈｅｍｅｃｈａｎｉｓｍｏｆｔｈｅｄｅｖｅｌｏｐｍｅｎｔｏｆｈｙｐｏｘｉｃｐｕｌｍｏｎａｒｙｈｙｐｅｒｔｅｎｓｉｏｎｉｓｎｏｔｃｌｅａｒＴｈｅｒｅｃｅｎｔｓｔｕｄｉｅｓｉｎｄｉｃａｔｅｄｔｈａｔｔｈｅｅｎｄｏｔｈｅｌｉｕｍｄｅｒｉｖｅｄｒｅｌａ...4|12|4Updated：2024-04-10
- Abstract：Objective.To study the experience of diagnosis and treatment of gastrinoma. Methods.A retrospective study of 17 patients with gastrinoma seen from 1978 through 1998 in PUMC hospital. Results.Three of the 17 cases were associated with multiple endocrine neoplasia type I（MEN I）syndrome.Tumors dispersed extensively in pancreas（31%）,duodenum（25%）,lymphnode（19%）and other place（25%）.Ultrasonography and computed tomography（CT）were sensitive in localizing tumor in pancreas and liver,but were not so well for small tumor in duodenum and lymph node.The angiography,percutaneous transhepatic portal sampling（PTPS）,scintigraphy and endoscopic ultrasonography（EUS）also can be used.Sixteen of 17 patients underwent 38 operations and 56 percent of the 16 cases underwent several operations.Seven patients at last performed total gastrectomy that was still considered as a choice of treatment.Tumor resection was rare because the advancing of tumor in most cases. Conclusions.Diagnosing gastrinoma in its early stage and striving for the tumor resection and reducing the metastasis of liver were the cruxes for good prognosis.4|16|0Updated：2024-04-10
- Abstract：ＷｅｈａｖｅｆｏｕｎｄｏｕｔａｎｅｗＰｖｕⅡｒｅｓｔｒｉｃｔｉｏｎｓｉｔｅｉｎａｐａｔｉｅｎｔｗｉｔｈｍｉｔｏｃｈｏｎｄｒｉａｌｅｎｃｅｐｈａｌｏｍｙｏｐａｔｈｙ，ＭｏｓｔｈｕｍａｎｍｉｔｏｃｈｏｎｄｒｉａｌＤＮＡ（ｍｔＤＮＡ）ｈａｓａｓｉｎｇｌｅＰ...4|4|0Updated：2024-04-10
- Abstract：Objective.To compare the effects of alfentanil and esmolol on hemodynamic and catecholamine response to tracheal intubation. Methods.Thirty five adult patients were randomly allocated to one of three groups,Group A（control group）,Group B（esmolol group）and Group C（alfentanil group）.The patients received either 2 mg/kg esmolol（in Group B）or 30 μg/kg alfentanil（in Group C）before intubation.Tracheal intubation was performed with 4 mg/kg thiopental and 0 1 mg/kg vecuronium and 3% isoflurane.Systolic blood pressure（SBP）,diastolic blood pressure（DBP）,mean blood pressure（MBP）,heart rate（HR）,norepinephrine（NE）,epinephrine（E）and dopamine（DA）were measured before and after intubation. Results.The control group had a baseline SBP of 149±23 mmHg while Groups B,C had a baseline SBP of 148±23,and 150±21mmHg,respectively（P>0 05）.Three min after tracheal intubation,the control group SBP increased to 160±30 mmHg and Group B remained at the baseline level,147±5 mmHg,and Group C significantly decreased to 91±22 mmHg（P<0 01）.Two min after intubation HR in Group B increased significantly but 3 min after intubation HR in Groups B and C were significantly lower than that of control group（P<0 05）.NE in Groups A and B increased significantly to 5 75±3 51 and 6 75±3 30 nmol/L 3 min after intubation（P<0 01）.In Group C,3 min after intubation NE was not significantly different from the baseline but E decreased significantly（P<0 01）. Conclusion.2 mg/kg esmolol can moderate the hemodynamic response to tracheal intubation to a certain extent and 30μg/kg alfentanil can completely attenuate the hemodynamic and catecholamine responses.4|10|2Updated：2024-04-10
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Cloning of Encoding Sequences for Chemokine Receptors CXCR4 and CCR5 from a Chinese Lymphocyte cDNAs
Abstract：Ｉｔｈａｓｂｅｎｋｎｏｗｎｒｅｃｅｎｔｌｙｔｈａｔｃｏｆａｃｔｏｒｓ，ｗｈｉｃｈｂｅｌｏｎｇｔｏｔｈｅｆａｍｉｌｙｏｆｓｅｖｅｎｔｒａｎｓｍｅｍｂｒａｎｅＧＴＰｂｉｎｄｉｎｇｐｒｏｔｅｉｎｃｏｕｐｌｅｄｒｅｃｅｐｔｏｒｓ，ａｒｅｎｅｃｅｓａｒｙ...4|8|0Updated：2024-04-10 - Abstract：ＣｈｉｎｅｓｅＡｃａｄｅｍｉｃＪｏｕｒｎａｌ（ＣＡＪＣＤ）ｉｓａｓｅｒｉｅｓｏｆｉｎｔｅｇｒａｔｅｄｆｕｌｔｅｘｔｄａｔａｂａｓｅｏｆｃｕｒｅｎｔｅｌｅｃｔｒｏｉｃａｃａｄｅｍｉｃｊｏｕｒｎａｌｓｉｎＣｈｉｎａＴｈｅｓｕｂｊｅｃｔａｒｅａｓｏｆＣ...4|2|0Updated：2024-04-10
- Abstract：Ｔｈｉｓｅｘｐｅｒｉｍｅｎｔｗａｓｄｅｓｉｇｎｅｄｔｏｅｘｐｌｏｒｅｔｈｅｉｏｎｉｃｂａｓｉｓｏｆｔｈｅａｂｎｏｒｍａｌｅｌｅｃｔｒｏｐｈｙｓｉｏｌｏｇｉｃａｌｃｈａｒａｃｔｅｒｏｆｔｈｅｍｙｏｃｙｔｅｓｆｒｏｍｔｈｅｒｉｇｈｔｖｅｎｔｒｉｃｌｅｏ...4|8|0Updated：2024-04-10
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