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Volume 0 ， Issue 1 ， 2000
- Abstract：WT5”BZ] To study the molecular mechanism of the stimulatory effect of low dose radiation（LDR） on T cell activation. [WT5”BX]Methods.[WT5”BZ] Thymocytes from Kunming mice exposed to whole body irradiation（WBI） with different doses of X rays were analyzed for the changes in signal molecules of the phospholipase C phosphatidylinositol biphosphate（PLC IP2） and G protein adenylate cyclase（AC） pathways. [WT5”BX]Results.[WT5”BZ]It was found that[Ca 2+ ] i increased in response to doses within 0 2 Gy which was most marked after 0 075 Gy and the increase was accentuated in the presence of Con A. The changes in CD3 and calcineurin（CN） expression of the thymocytes followed the same pattern as the alterations in [Ca 2+ ] i after LDR. The expression of α,β1 and β2 isoforms of protein kinase C（PKC） was all up regulated after 0 075 Gy with the increase in PKC β1 expression being most marked. The cAMP/cGMP ratio and PKA activity of the thymocytes was lowered after low dose radiation and increased after doses above 0 5 Gy in a dose dependent manner, thus giving rise to J shaped dose response curves. The Ca antagonist TMB 8 and cAMP stimulant cholera toxin suppressed the augmented thymocyte proliferation induced by LDR. [WT5”BX]Conclusion.[WT5”BZ]Data presented in the present paper suggest that activation of the PLC PIP2 signal pathway and suppression of the AC cAMP signal pathway are involved in the stimulation of the thymocytes following WBI with low dose X rays.Keywords：WT5BZ]low dose radiation;intracellular Ca2+;PKC isoforms;cAMP/cGMP ratio4|17|3Updated：2024-04-10
- Abstract：To study the effects of XW630 on bone formation in overiectomized（OVX） rats and in human osteoblast like cell line TE85. [WT5”BX]Method.[WT5”BZ] Bone histomorphometric analysis was performed with undecalcified bone sections and tetracycline intraperitoneally labeling. [WT5”BX]Results.[WT5”BZ] Compared with that of OVX rats, the static data of trabecular bone volume（TBV）/ total tissue volume（TTV）, TBV/sponge bone volume（SBV） and mean trabecular plate density （MTPD） were enhanced while mean trabecular plate spacing（MTPS） decreased after treated with XW630 for 13w. The dynamic data of single labeled surface [Sfract（s）], double labeled surface[Sfract（d）],Sfract（d+1/2s）,trabecular osteoid surface（TOS）, and bone formation rate in tissue level （Svf） were increased and osteoid maturation period （OMP） shortened in XW630 group. In osteoblast like cells, both 3H thymidine incorporation and cell count increased after treated with XW630 for 48. Treated with XW630 for 12～18h,inducible nitric oxide synthase（iNOS） activity and cGMP content increased in time dependent manners. [WT5”BX]Conclusions.[WT5”BZ] XW630 enhanced bone activation frequency and increased trabecular connectivity, stability, and strength. The cellular mechanism related to effects of XW630 on bone formation in ovariectomized rats.Keywords：WT5BZ]XW630;ovariectomy;osteoblast4|24|0Updated：2024-04-10
- Abstract：To evaluate how the efficacy of DNA inocutation affects the ability to raise protective immunity against Leptospira. [WT5”BX]Methods.[WT5”BZ] A pair of oligonucleotide primers were designed to amplify the endoflagellar gene of L. interrogans sensu stricto serovar lai. An approximately 840bp fragment was generated with PCR and inserted into VR1012, a plasmid DNA expression vector, after the fragment and VR1012 were digested respectively with EcoRV and Sal I. A recombinant plasmid designated as VR1012+flaB2 was obtained. The vector, VR1012 consits of a pUC18 backbone with the cytomegalovirus（CMV） IE1 enhancer, promoter, and intron A, transcription regulatory elements and the BGH polyadenylation sequences driving the expressing of leptospiral endoflagellar gene of L. interrogans sensu stricto serovar lai. Plasmid encoding leptospiral endoflagellin gene was injected into quadriceps of NZW rabbits. [WT5”BX]Results.[WT5”BZ]This resulted in the generation of specific leptospiral antibody with high ELISA titer （1:32768） in the rabbits. Immuno/protection was performed in guinea pigs without adjuvant. The group“VR1012+flaB2” showed higher survival rate（90%,9/10 animals）,compared with the group “VR1012 lack flaB2” and the group “normal saline”. [WT5”BX]Conclusion.[WT5”BZ]The technique of DNA vaccine has potential advantages over certain other vaccine preparation technologies. However whether DNA vaccine will be useful for vaccine development remains to be tested.Keywords：leptospira;endoflagellin;DNA vaccine4|7|1Updated：2024-04-10
- Abstract：In order to understand the role of transmembrane signal transduction of host cells in the early steps of infection,the adherence of E. coli to HEp 2 cells and the change of activity of phospholipase C γ （PLC γ） induced by the adherence were investigated.The adherence of enteropathogenic E.coli （EPEC）, strain E.7, induced a significant increase of inositol triphosphat （IP 3） level in HEp 2 cells. The adherence of the bacteria and the increase of IP 3 was kinetically correlated. Whereas the increase of IP 3 level induced by the adherence of the control strain EPEC （H511）, a non piliated strain, was much meager than that by E7, a piliated strain. The results highlighted an important role of transmembrane signals like IP 3 in the pathogenesis of EPEC.Keywords：inositol triphosphate;entero- pathogenic E coli;adherence4|23|0Updated：2024-04-10
- Abstract：Angiotensin converting enzyme （ACE） plays a key role in the metabolism of angiotensin Ⅱ （AT Ⅱ） and inactivation of bradykinins and tachykinins, which are potent bronchialconstrictors and mediators of inflammation asthma, and ACE is heavily expressed in the lungs. An insertion deletion （D/I） polymorphism of ACE gene has been shown to be associated with levels of ACE. We investigate whether the polymorphism of ACE gene is associated with asthma and bronchial responsiveness. Methods. A case control study was carried out in 50 asthmatics, 7 families with at least 2 asthmatic individuals, and 50 healthy subjects. The insertion/deletion （I/D） polymorphism of ACE gene was amplified by polymerase chain reaction （PCR）. Methacholine brocho provocation and pulmonary function tests were performed in all asthmatics. Results. There was an higher gene frequency of DD genotype of ACE gene in asthmatic subjects and families individuals compared with healthy subjects （46%, 53% vs 16%, P<0 05; odd ratio 4 98）. Anhigher prevalence of DD genotype of ACE was in patients with bronchial hyperresposiveness （BHR） （67%vs 33%, P<0 05; odd ratio 3 8）. Accordingly, the mean values of FEV 1% and FEV 1/FVC were higher in asthmatics carrying non DD alleles than patients with DD genotype （73 78% vs 56 56%, P<0 05; 79 19% vs 69 29%, P<0 05, respectively）. Conclusion. These results suggested that DD allele of ACE genotype was significantly involved in genetic susceptibility to asthma. DD genotype of ACE might be a risk factor for the degree of airway obstruction, it could also be implicated in pathogenesis of bronchial hyperresponsiveness.Keywords：asthma;genetics;polymorphism;angiotensin converting enzyme6|27|2Updated：2024-04-10
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- Abstract：This study sought to investigate whether low dose dobutamine MRI can detect residual myocardial viability in patients with chronic myocardial infarction and left ventricular dysfunction. Methods. Eleven patients with chronic myocardial infarction and left ventricular dysfunction were employed for identification of viable myocardium by cine MRI during dobutamine infusion. All patients underwent coronary angiography and left ventriculography, 18 FDG PET, MRI at rest and stress.The systolic wall thickening measured at rest and during stress was compared with the results of 18 FDG PET, respectively. Results. A significant difference of either dobutamine induced systolic wall thickening （SWth stress ） or dobutamine induced contractile reserve （ΔSWth= SWth stress - SWth rest ） was present between viable and scar regions （1 0±0 3 versus -0 3 ±0 1, P<0 01; 1 0±0 3 versus -0 2±0 2, P<0 01）. Conclusions. Dobutamine induced contractile reserve can be predicted in the regions of akinesia or dyskinesia at rest when systolic wall thickening was 1 0 mm during dobutamine stimulation.Keywords：myocardial infarction;inotropic agents;stress4|15|1Updated：2024-04-10
- Abstract：BZ]Dendro dendritic and dendro somatic projections are common between spinal motoneurons. We attempted to clarify whether there are functional connections through these projections. Methods. Motoneurons were antidromically stimulated by the muscle nerve and recorded intracellularly to examine the direct interaction between them, after the related dorsal roots had been cut. Results. Excitatory connections, demonstrated by depolarizing potentials in response to muscle nerve stimulation, were found between motoneurons innervating the same muscle or synergistic muscles, but never between motoneurons innervating antagonistic muscles. These potentials were finely graded in response to a series of increasing stimuli and resistant to high frequency （50Hz） stimulation. Conclusions.These results indicate that excitatory connections, with certain specificity of spatial and temporal distribution, occur in the spinal motoneurons. It is also suggested that electrical coupling should be involved in these connections and this mechanism should improve the excitability of the motoneurons in the same column.Keywords：motoneuron;dendritic projection;gap junction;excitatory connection4|28|9Updated：2024-04-10
- Abstract：BZ]To investigate the exact mechanism of melatonin to prohibit the apoptosis of neural cells induced by various kinds of cytotoxic agents. [WT5”BX]Methods. [WT5”BZ]We used the methods of phase contrast microscopy, MTT assay and hoechst dye staining to check this mechanism in SKNSH and U251 cell lines. [WT5”BX]Results. Both 2mmol/L H 2O 2 and 0 5 μ mol/L amyloid β protein （Aβ） induce these two cell lines die via apoptosis. Either melatonin or glutathione can significantly protect both cell lines. The protective effect of 10 μ mol/L melatonin is as same as that of 60 μ mol/L glutathione. [WT5”BX]Conclusion. Melatonin can partly inhibit the cytotoxicity of H 2O 2 and Aβ through its role as a free radical scavenger.Keywords：melatonin;apoptosis;amyloid β protein;cytotoxicity6|24|1Updated：2024-04-10
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- Abstract：To evaluate the role of hysterectomy for patients with gestational trophoblastic tumor. [WT5”BX]Methods.[WT5”BZ]We retrospectively analyzed 68 cases of gestational trophoblastic neoplasia treated by hysterectomy from 1985～1997 at PUMC hospital. Thirty eight cases were diagnosed of choriocarcinoma and 30 were invasive mole. [WT5”BX]Results.[WT5”BZ]Twenty three elder patients who didn’t desire to preserve fertility were selected for hysterectomy after shorter courses of chemotherapy, 22 of them had a complete remission（95 6%）, the total aver age courses of chemotherapy was 4 2. Of twenty seven chemorefractory cases who were suspected of a refractory isolated lesion in the uterus, delayed hysterectomy as an adjunct to chemotherapy was performed, 20 of them got a complete remission（74 1%）, the total average courses of chemotherapy were 9 4. Emergency hysterectomy is indicated in 18 patients with uterine perforation or life threatening hemorrhage, 17 cases had a complete remission（94 4%）, the total average courses of chemotherapy were 7 6. [WT5”BX]Conclusion.[WT5”BZ]Although the development of effective chemotherapy has resulted in improved survival of patients with gestational trophoblastic tumor, hysterectomy remains an important adjuncts in the treatment of a selected subset of patients; in order to operate more completely and prevent recurrence, it’s better to perform extended hysterectomy for the indicated patients.Keywords：trophoblastic tumor;hysterectomy4|41|5Updated：2024-04-10
- Abstract：To evaluate the treatment results of bilateral alveolar bone grafting （BABG） in patients with bilateral complete clefts of lip and palate. [WT5”BX] Methods.[WT5”BZ] A retrospective study was performed in 66 bilateral complete cleft lip and palate patients who received the procedure of BABG, among them 15 were primary BABG and 51 were secondary BABG. The patients were further divided into three groups according to age and eruption stage of the canine at the time of surgery. The result of BABG was evaluated on the radiographs. [WT5”BX] Results.[WT5”BZ] （1）The overall success rate of BABG was 75 0%, with 83 3% and 72 5% for primary and secondary BABG respectively; （2）The marginal bone level was found to be significantly higher in the youngest age group than in the other groups both for primary and secondary BABG; （3）For both primary and secondary BABG, Group C （patients’ age more than 16 years） had the least optimal success rate, with 66 7% and 65 4% respectively. [WT5”BX] Conclusion.[WT5”BZ] Simultaneous primary palate repair and BABG is safe and feasible procedure for treating unoperated bilateral complete cleft lip and cleft palate patients. For both primary and secondary BABG, significantly better results can be achieved if the operation is performed before eruption of the canine.Keywords：cleft lip and palate;alveolar bone grafting4|30|0Updated：2024-04-10
- Abstract：The aim of this paper is to describe the mechanism, clinical manifestation, diagnosis and surgical management of congenital choledochal cyst. [WT5”BX] Methods.[WT5”BZ] From 1984 to 1997, 56 cases of congenital choledochal cyst were reviewed. Among them, 8 patients were male, 48 patients were female, the age ranges from 12 to 50 years old with an average of 26 3. [WT5”BX] Results.[WT5”BZ]The main clinical manifestation includes jaundice, abdominal pain and abdominal mass. All of them were confirmed by ultrasonography, while 39 cases were performed ERCP and PTC with the same diagnosis. Fifty one patients were performed cystectomy and hepatojejunostomy, two cases were performed cystjejunostomy because of diffused angioma and severe hemorrhage respectively, external drainage was performed in one case with emergent cholangitis, the other two cases were reported malignancy through biopsy and operation was abandoned. [WT5”BX] Conclusions. [WT5”BZ]Ultrasound diagnostics is essential to accurately diagnose the cyst, preoperative ERCP is helpful for differentiating pancreatic duct from bile duct, while MRCP is a reliable method; cystectomy and cholangiojejunostomy is recommended, laparoscopic procedure is becoming more and more accepted.Keywords：congenital biliary cyst;hepatobiliary anastomosis4|9|1Updated：2024-04-10
- Abstract：To introduce the epidemical, pathological, and clinical characteristics as well as the diagnostic and therapeutical experiences of endomyocardial fibrosis（EMF） in China. [WT5”BX] Data sources.[WT5”BZ]A CMBdisc search was done of the Chinese language literature published from January 1983 through June 1997 about EMF and/or restrictive cardiomyopathy. A manual search was then done for other contributions, including abstracts, between January 1965 and June 1997. [WT5”BX] Results. [WT5”BZ]Eighty seven Chinese cases of EMF were collected in this paper. There were 49 men and 38 women, with a mean age of 28±13 years（range, 8 to 68 years）. The distribution of the cases is mainly in the south of China. Combined right and left ventricular disease occurs in 48 percent of cases, with pure right ventricular involvement occurring in 42 percent and pure left ventricular involvement in the remaining 10 percent of patients who are examined postmortem. The diagnosis of EMF was confirmed in 21 cases at autopsy, and in 66 cases by echocardiography, angiocardiography, and/or endomyocardial biopsy which showed the characteristic changes. Clinically, right sided disease is the commonest variety. Endocardiectomy and tricuspid（n=7） or mitral（n=1） valves replacement have been performed in 8 patients. There were 2 operative deaths. Six patients had a satisfactory recovery postoperatively and living well in the follow up duration. [WT5”BX] Conclusion.Keywords：EMF has been diagnosed clinically and confirmed at necropsy in a number of cases in the south of China. The etiology;incidence and epidemiology are still unknown. The pathological and clinical features are simil4|16|3Updated：2024-04-10
- Abstract：To delineate the clinical spectrum and treatment choice of sacral fractures. [WT5”BX]Methods.[WT5”BZ] In this series, 39 sacral fractures were retrospectively reviewed and classified utilizing Denis′ classification. There were 21 Zone Ⅰ fractures, 6 Zone Ⅱ fractures and 12 Zone Ⅲ fractures. Neurological deficits were present in seven patients. Thirty seven patients were treated conservatively and two underwent surgical management. [WT5”BX]Results.[WT5”BZ]Thirty eight patients were followed up for three months to 19 years. Thirty three have recovered, four improved, and one remained disabled. [WT5”BX]Conclusion.[WT5”BZ]The treatment of sacral fractures requires assessment of pelvic stability and existing nerve injury. The patients with pelvic ring instability and neurological deficits should be treated with fracture reduction and stability reconstruction. When the patients with pelvic fracture are complicated with neurological deficits, sacral fracture should be first suspected. Once the diagnosis of sacral fracture is made, fracture reduction should be indicated. Conservative treatment usually permits satisfactory results.Keywords：sacrum;fractures4|28|0Updated：2024-04-10
- Abstract：The aim of this study was to detect coronary artery disease using 99m Tc MIBI myocardial perfusion imaging in patients with valvular disease. [WT5”BX]Methods.[WT5”BZ] Thirty patients with valvular disease confirmed by echocardiography underwent 99m Tc MIBI myocardial perfusion imaging using multiSPECT 1h after stress test （exercise, dipyridamole or dobutamine test） and were performed coronary angiography within 1 month before valvular operation. [WT5”BX]Results.[WT5”BZ]For 29 out of the 30 patients, the results of 99m Tc MIBI myocardial perfusion imaging were similar with those of coronary angiography, the concordance rate was 96 7% and the negative predictability was 100%. [WT5”BX]Conclusion.[WT5”BZ] 99m Tc MIBI myocardial perfusion imaging is a reliable non invasive method for detecting coronary artery disease in patients with valvular disease and so as to draw up suitable operation programs for them.Keywords：valvular disease;coronary artery disease;radionuclide imaging4|15|1Updated：2024-04-10
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