Latest Issue
Volume 0 ， Issue 2 ， 2000
- Abstract：Objective. To make a preliminary investigation of the patency and function of coronary artery bypass grafts（CABG） by magnetic resonance（MR） images and to establish a suitable method for followup study after CABG operation among Chinese. Methods. MR imaging was performed with a Toshiba 15T unit in 27 patients with 74 grafts. All patients were examined with a breathhold ECGgated twodimensional fast field echo （FFE） sequence to evaluate the patency of bypass grafts, among them 16 patients with 42 grafts were further examined with a phase shift magnetic resonance angiography flow （PSMRAflow） sequence to evaluate the grafts patency as well as the flow velocity and flow volume vs. time. Results. The results showed that 66 of the 74 grafts in the patients of the present series studied with FFE were patent with a patency rate of 892% The results evaluated both with FFE and PSMRAflow remained the same except that two grafts were patent with FFE and the results with PSMRAflow were uncertain. Diastolic perfusion pattern curves were found in 25 of the 32 grafts in patients of the present series. Comparing the flow curves of the grafted left internal mammary artery with those of the native right internal mammary artery in 7 patients, the systolic peak velocity value（SPV） of the grafted arteries was significantly lower than that of the ungrafted ones, whereas the diastolic peak velocity value（DPV） and the ratio of DPV to SPV were significantly greater than that of the ungrafed ones. Conclusion. The FFE and PSMRAflow sequences were efficient in evaluating patency and obtaining the curves of flow velocity and volume of the bypass grafts. Therefore, they may offer a noninvasive screening method for followup study in patients after CABG surgery, although its accuracy should be further evaluated in more patients and comparatively studied with other methods.Keywords：coronary artery bypass;magnetic resonance imaging;flow4|7|0Updated：2024-04-10
- Abstract：Objective.In a model of balloon injury of rat aortic endothelium, the effects of C-type natriuretic peptide（CNP） on α 1-adrenoreceptor and inositol 1,4,5-triphosphate （IP 3） receptor were studied Methods. Aortic injuries were produced by vascular endothelium-denudation.α 1- adrenoreceptor in smooth muscle sarcolemma and IP 3 receptor in smooth muscle sarcoplasmic reticulum in the rat aorta were assayed by radioactive analysis method Results. It was found that neointima was formed and the contents of DNA, collagen and elastin of each intimamedia were significantly increased in 7 days and 21 days after balloon injury of rat aorta. α 1adrenoreceptor in smooth muscle sarcolemma and IP 3 receptor in sarcoplasmic reticulum were also upregulated. Results also showed that the administration of CNP ip significantly decreased the contents of DNA, collagen and elastin of each intimamedia, and inhibited the upregulation of α 1adrenoreceptor and IP 3 receptor Conclusion. The inhibition of the upregulation of α 1adrenoreceptor and IP 3 receptor by CNP might be one of the mechanisms of its suppressive action on intimal proliferationKeywords：restenosis;balloon angioplasty;C-type natriuretic peptide4|21|0Updated：2024-04-10
- Abstract：Objective. The goal of this paper is to investigate the relationship between the N-glycosylation of acetylglucosaminyltransferase V（Glc NAcT-V） and its activity and to know which site among the 6 N-glycosylation sites in the GlcNAcT-V gene is the most important. Methods.Wild type of GlcNAcTV was transfected into COS7 cells and its activity was measured 48 h later. The first site （Asn 110） was mutated with sitedirected mutagenesis and transfected into COS7 cells. Results. It was found that after the cells were added tunicamycin（TM, 1 μ g/ml）, the activity was 117% of the wild type. The activity of the cells with mutating GlcNAcTV was about 120% of the wild type RTPCR showed that there was no significant change in mRNA expression among the three groups. Conclusion.The Nglycosylation is important for its activity. Our results suggest that the Nlinked carbohydrates on GlcNAcTV are required for the posttranscriptional activity of the enzyme.Keywords：GlcNAcT-V;glycosylation site;mutagenesis4|20|0Updated：2024-04-10
- Abstract：Objective. To study the effects of two kinds of Chinese herbal medicine, Radix angelicae sinensis（RAS）（当归）and Shuanghuanglian（SHL）（双黄连） on chronic Pseudomonas aeruginosa（PA）lung infection in a rat model mimicking cystic fibrosis（CF）. Methods.Rats were divided into RAS, SHL and control groups. All rats were challenged intratracheally with alginate embedded PA and the treatments with herbal medicine started on the same day of challenge. The drugs were administered subcutaneously once a day for ten days and the control group was treated with sterile saline.The rats were sacrificed two weeks after challenge. Results. Significantly improved lung bacterial clearance（P<005, P<001） and milder macroscopic lung pathology （P<0005） were found in the two treated groups compared to the control group. In the SHL treated group, the neutrophil percent in the peripheral blood leukocytes（P<005）, the antiPA IgG level in serum （P<005）, the incidence of lung abscesses（P<0005） and the incidence of acute lung inflammation（P<005） were significantly lower than in the control group. The RAS treatment reduced fever（P<005）, decreased the incidence of lung abscesses（P<0005） and lung mast cell number （P<005）, and lowered antiPA IgG1 level in serum（P<005） when compared to the control group. The antiPA bacterial activity test in SHL was weakly positive whereas in RAS it was negative. Conclusion.The treatment with both herbal medicines could increase the resistance of the rats against PA lung infection and they therefore might be potential promising drugs for stimulation of the immune system in CF patients with chronic PA lung infection.Keywords：cystic fibrosis;chronic P. aeruginosa pneumonia;Chinese herbal pharmacology4|42|5Updated：2024-04-10
- Abstract：bjectives. To investigate the role of endogenous heme oxygenase （HO）/carbon monoxide （CO） system in regulating the process of intussusception （IN） induced by administration of lipopolysaccharide （LPS） in rats. Methods. IN model of rats were induced by lipopolysaccharide. HO activity was determined by the amount of bilirubin formation which was measured with a doublebeam spectrophotometer, and HbCO formation was measured by COoximeter. Results. The results showed that LPS （10mg/kg） caused IN in up to 40% of the rats at 6h after treatment of LPS. The incidence of IN were significantly increased by 50% （P<005） and by 832%（P<001） in HO substrate（hemeLlysinate）treated rats and in exogenous COtreated rats, respectively; but it was significantly decreased by 418%（P<005） after administration of ZnDPBG, an inhibitor of heme oxygenase （HO） activity. Furthermore, LPS increased HO activity, HbCO formation cGMP content within colic smooth muscle and the plasma level of cGMP, and these parameters were significantly elevated by 626%（P<001）, 400%（P<001）, 493%（P<005） and 38%（P<005）, respectively, compared with LPSnonIN rats. Conclusion. It is suggested that endogenous HO/CO system plays an important role in the process of IN induced by LPS, and inhibition of HO activity may decrease the formation of IN.Keywords：heme oxygenase;carbon monoxide;intussusception4|14|1Updated：2024-04-10
- Abstract：Objective. To approach the relation and the possible mechanism between the expression of intercellular adhesion molecule （ICAM1） mRNA and acute lung injury （ALI） and the mechanisms of rhubarb in the prevention and treatment of the lung injury. Methods. Lipopolysaccharide （LPS） was injected into the sublingual vein of male Wistar rats to perform ALI animal model. The rats were divided into 4 groups: LPS group, control group, rhubarb group and dexamethasone group. Macroscopic and histopathological examinations were performed and biological markers were measured for the lung specimens. The markers included lung wet/dry weight, the rate of neutrophils and protein content in the pulmonary alveolar lavage fluid, pulmonary vascular permeability and pulmonary alveolar permeability index. Molecular hybridization method was used to determine the expression of ICAM1 mRNA. Results. In the lung tissues, the ICAM1 mRNA expression was increased in the endothelial cells of pulmonary veins and capillaries, rhubarb and dexamethasone had the action of decreasing the expression. The light reflex value in the gray scale scanning showed that in the comparison between the LPS and the control group, the gray scale value of the lung tissues in ALI was significantly increased, thus the light reflex value was markedly decreased （P<001）, demonstrating the expression of ICAM1 mRNA was increased. In comparison with the LPS group, dexamethasone and rhubarb could decrease the gray scale value of the lung tissue significantly, thus the light reflex value was elevated （P<001, P<005）; the corresponding pathologic changes of lung tissues and the biological markers of the lung injury were significantly decreased or ameliorated. Conclusions. The increase of the expression of ICAM1 mRNA in the lung tissues of ALI plays the roles in ALI. The application of rhubarb and dexamethasone can decrease the expression and ameliorate the lung damage; its mechanism is possibly via the inhibition of ICAM1 mRNA expression.Keywords：aucte lung injury;rhubarb;intercellular adhesion molecule4|31|9Updated：2024-04-10
- Abstract：Objective.To study the role of endogenous carbon monoxide （CO） in endotoxin shock. Methods. The changes of CO levels and the effects of zinc protoporphyrin IX （ZnPP）,an inhibitor of hemeoxygenase （HO）, in endotoxin shock and the efficacy of hemin,an inducer of HO were investigated. Results.The plasma CO levels were found to be significantly increased during the course of endotoxin shock. Injection of ZnPP was shown to abrogate the endotoxininduced hypotension and metabolic derangements markedly. Administration of hemin to healthy rabbits revealed the hypotension and metabolic derangements similar to the animals given endotoxin. Conclusion.CO is a newly found endogenously produced mediator which may play an important role in the pathogenesis of endotoxin shock.Keywords：carbon monoxide;endotoxin;septic shock4|18|0Updated：2024-04-10
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- Abstract：Objective.Analysis of the cause of intrauterine death of one fetus of twin and to evaluate the outcome of conservative management of twin pregnancy with single fetal death. Method.A retrospective review was undertaken on PUMC hospital records of women who delivered twin between Jan. 1987 and Dec. 1998. Result.During the recent 12 years, 99 pregnant women were documented as twin pregnancies. Of the twin pregnancies during this period, 13 were complicated by a single intrauterine death. Four women suffered this complication during their first trimester. In the remain 9 cases one fetus died during second or third trimester. The cesarean section was given in three pregnant women soon after a single intrauterine death because of the survival fetal distress. No consumptive coagulopathy was apparent in all 13 pregnant women. It is wise that the pregnancy was managed conservatively, with regular coagulation parameters obtained. Conclusion.Our results support conservative management in twin pregnancies complicated by single fetal death.Keywords：twin pregnancy;fetal death4|22|0Updated：2024-04-10
- Abstract：Objective.To analyse the efficiency of two operations for treatment of unirary stress incontinence （USI）. Methods. The clinical data of 95 USI cases or accompany USI treated by surgical procedures （Kelly operation 63 cases, Colposuspension 32 cases） was analysed retrospectively in our department. Results.The cure rates during three months after operation were 92.9% in Colposuspension group and 68.9% in Kelly operation group （P<0.05）, and decreased to 73.7% and 57.5% one year after operation in Colposuspension group and Kelly operation group respectively （P>0.05）. Abnormal senses rates of sexual intercouse was 3.7% in Cloposuspension and 7.3% in Kelly operation group. The length of retaining Folly catheter after Colposuspension was more than Kelly operation. [WT5”BX]Conclusion.[WT5”BZ]The cure rate during three months after Colposuspension is better than Kelly operation. But the cure rates during one year after surgery are the same in two groups.Keywords：unirary stress incontinence;Kelly operation;colposuspension6|46|0Updated：2024-04-10
- Abstract：To observe the clinical outcomes of using a new instrumentation SOCON system in the treatment of degenerative spondylolisthesis. Methods. Retrospective clinical and roentgenograph review of 21 patients who suffered from degenerative spondylolisthesis with spinal stenosis treated by decompression, posterolateral intertransverse arthrodesis, and with transpedicle instrumentation of SOCON system. Results.Nineteen of 21 patients were completely recovery from their preoperative symptoms, 17 of 18 cases with grade one slippage reduced to normal spine alignment, 2 cases of grade two slippage fully reduced, and one case of grade two spondylolisthesis got 70% reduction. Post operative satisfactory rate was 90.5%. Pain relief was 90.5%. Neither infection nor neurologic complication occurred in this series. Conclusion. Our short time followup and limited cases showed satisfactory preliminary result of surgical treatment of spondylolisthesis with SOCON instrumentation.Keywords：spondylolisthesis;surgical treatment;SOCON instrumentation4|34|0Updated：2024-04-10
- Abstract：Objective. To improve the recognition of diagnosis and treatment of gallbladder cancer. Methods. Retrospective analysis of 52 cases of gallbladder carcinoma in our hospital from 1988 to 1998. Results. Preoperative diagnostic rate was 90.3%, of which 12 cases （23%） were early stage of carcinoma. The total operation resection rate was 55.8%, for which only 17.8% were advance stage of carcinoma. Conclution.The early diagnosis is the key factor of increasing treatment successful rate. The following are the symptoms that raise our special attention to carcinoma of gallbladder: （1） Age over 50 have recurrent cholecystitis and with past history of gallstone; （2） Congenital malformation of bile duct; （3） Local thickening and irregularity of gallbladder wall; （4） Polypoid lesion larger than 1cm inside gallbladder; （5） Atrophic gallbladder; （6） Intraluminal stone of gallbladder does not move when change in body position; （7） Regional lymph node enlargement.Keywords：gallbladder carcinoma;radical resection;anomaly6|19|0Updated：2024-04-10
- Abstract：Objective. In order to investigate 5,10-methylenetetrahydrofolate reductase （MTHFR）s polymorphic changes in psoriasis vulgaris. Methods.We detected mutation of site C677V of MTHFR in 39 psoriastics by PCR-RFLP. Results.Genotype frequencies of the psoriastics were C/C=20.15%,C/T=48.72% and T/T= 30.77%; the allelic frequencies were C=0.4487 and T=0.5513. Homozygous mutant （TT） of the psoriastics was significantly different from the normal control group by X2 test. Conclusion.C677V mutant of MTHFR might be related with psoriasis.Keywords：MTHFR;PCR-RFLP;psoriasis;gene mutation4|20|0Updated：2024-04-10
- Abstract：Objective. Report of first case of Protothecosis zopfii in China and causes the skin infection in the world. Method.By clinical and laboratory examinations to confirm the diagnosis and the response to treatment. By the review of literatures to confirm the first case of human skin infection in the world. Result.From the literatures and the clinical pictures, it is confirmed that this is the first case report of Protothecosis zopfii of skin in the world. Conclusion.The first case of Protothecosis zopfii in human being was reported and successfully treated with local infiltration of Diflucan （fluconazole） 3ml （2mg/ml）/week×4.Keywords：protothecosis zopfii;prototheca zopfii;fluconazole4|29|5Updated：2024-04-10
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- Abstract：Objective. This study is to investigate the functional mimicry by using antiidiotypic antibodies of enzymes. Methods.Monoclonal antiidiotypic antibodies against antiHEL（hen eggwhite lysozyme, HEL） antibodies were obtained by fusion of Sp2/0 myeloma cells with spleen cells of syngeneic mice immunized with monoclonal antiHEL antibodies against HELs different antigenic epitopes. Then bacteriolysis of the antiidiotypic antibodies were observed. Results.Eight hybridomas strains secreting antiidiotypic antibodies were selected and characterized. It was shown that two of eight antiidiotypic antibodies secreted by two hybridomas（1A 10 C 9 and 2A 11 C 1B 3） could mimic HEL catalytic activity to lyse Micrococcus lysodeikticus and that the catalytic effect of mixed antiidiotypic antibodies of 1A 10 C 9 and 2A 11 C 1B 3 was stronger than that of one of them, but less than HEL. Conclusion. The results demonstrated that the antiidiotypic antibodies that could mimic enzyme activity existed in the idiotype network during antienzymatic immune response.Keywords：anti-idiotypic antibody;functional mimicry;hen egg-white lysozyme4|23|0Updated：2024-04-10
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