Latest Issue
Volume 0 ， Issue 3 ， 2001
- Abstract：Objective. To study the effect of interleukin 10 （IL- 10） on the angiotensin II （AngII） stimulated rat VSMC proliferation and collagen secretion, and furthermore, explore its mechanism. Methods. On cultured VSMC of rat, 3H- thymine （3H- TdR） and 3H- proline incorporations were used to evaluate the DNA and collagen synthesis, respectively. Western blot and immunoprecipitation were applied to assay the expression and activity of focal adhesion kinase （FAK）, respectively. Results. IL- 10 （10－ 8～ 10－ 10g/ml） inhibited the increase of 3H- TdR and 3H- proline incorporation as well as FAK activity, which was induced by 10－ 7mol/L AngII （P< 0.05 or P< 0.01）. IL- 10 also obviously downregulated the synthesis and secretion of collagen by AngII stimulated VSMC. But there was no difference in the protein expression of FAK among all the groups （P >0.05）. Conclusion. IL- 10 antagonizes the VSMC proliferation and collagen synthesis by regulating FAK activity stimulated by AngII.Keywords：interleukin 10;angiotensin II;focal adhesion kinase4|18|2Updated：2024-04-10
- Abstract：Objective. Using a recombinant human adenovirus to express modified VP4 gene of rotavirus SA11 strain. Methods. A whole VP4 gene was obtained with PCR and induced the signal peptide at the gene N terminal. The chimera gene was cloned into pCMV plasmid that consists of human cytomegalovirus promoter, and then the gene was cloned to the transfer vector of human adenovirus type 5. Homologous recombination was performed by co- transfection to 293 cell lines with recombinant plasmid and viral genome using CaPO4 precipitation. Results. No mutation was found in the whole VP4 gene sequence of 2362 base pair. The expressed product in recombinant adenovirus was confirmed to be specific and more antigenicity by indirect immunofluorescence assay. Both the Western blot and immunoprecipitation assay showed that the molecular mass of the expressed protein was higher than the wild type VP4 protein, and that the modified product was corresponding to a glycosylation of VP4 protein. Conclusion. To modify the target gene might be an effective method to enhance the stability, antigenicity and immunogenicity of expressed protein.Keywords：rotavirus;recombinant adenovirus;glycosylation4|8|0Updated：2024-04-10
- Abstract：Objective.To study the mechanism and effects of blood perfusion on acute ischemic region of myocardium through channel created by Ho- Yag laser and True- cut biopsy needles with myocardial contrast echocardiography. Methods. We partially ligated the left anterior descending coronary artery of canine hearts between the lst and 2nd diagonal branches to produce two groups of acute myocardial ischemia models and then performed tran- smyocardial revascularization （TMR） on this region with Ho- Yag laser and True- cut biopsy needles. Myocardial contrast echocardiography was performed with a new generation of ultrasound contrast agent and second harmonic imaging of this region before, during ischemia and after revascularization. Pictures were taken with“ R” wave trigger skill. Results. Acoustic density （dB） in the ischemic region （anterior wall） with myocardial contrast echocardiography decreased obviously after the left anterior descending artery was ligated （Laser group: 5.40± 1.81, Needle group: 7.11± 2.51） compared with that before （Laser group: 11.69± 1.61, Needle group: 12.96± 2.88, P< 0.01）. dB increased remarkably after TMR by either laser or True cut biopsy needle （Laser group: 11.02± 2.01, Needle group: 10.01± 4.45. P< 0.01） compared to that during ischemia and approximated to that before ischemia （P >0.05）. We found that the acoustic density of the contrast developed one picture （one cardiac cycle） ahead in the transmyocardial revascularization region than that in the lateral and other region of the left ventricle wall in the scan of both groups. Conclusions. Acute ischemic myocardium can be perfused immediately by oxygenated blood from the left ventricle through channels created with both Ho- Yag laser and True- cut biopsy needles. Evidence of blood perfusion through these channels mainly during systolic phase was detected, and myocardial contrast ultrasound using intravenous perfluorocarbon- exposed sonicated dextrose albumin was regarded as a reliable method in the study of transmyocardial revascularization.Keywords：Ho- Yag laser;True- cut biopsy needle;transmyocardial revascularization4|11|0Updated：2024-04-10
- Abstract：Aim. To investigate the bioactivity of the self- designed biodegradable osteosynthetic devices made of resorbable hydroxyapatite microparticles/ poly- DL- lactide （HA/PDLLA） composites. Method. Forty- three rabbits with a transverse transcondylar osteotomy of the distal femur were fixed intramedullary by a HA/PDLLA rod, the duration of follow- up were 3， 6， 12, 24 and 36 weeks. Histological, scanning electron microscopic （SEM）, energy dispersive X- ray （EDX） and biomechanical analyses were done. Results. Active new bone formation and direct bone- bonding were seen at the bone- implant interface. Generous apatite crystals deposited and grew on the surface of the composites at 3～ 6 weeks postoperation. The interfacial shear strength increased significantly. Conclusion. Through the incorporating of resorbable HA microparticles, specific bone- bonding and active osteogenic capacity is introduced. This kind of bioactivity, together with other properties such as sufficient mechanical strength, enhanced biocompatibility and radiopacity, which are intrinsically unobtainable in totally resorbable polymer/polymer systems, make the HA/PDLLA composites become a desirable material for the internal fixation of cancellous bone.Keywords：bioresorbable material;osteosynthetic material;bioactivity4|20|5Updated：2024-04-10
- Abstract：Objective. To investigate the anti- tumor effects of human single chain interleukin- 12 （hscIL- 12）. Method. pcDNA/ hscIL- 12 recombinant was transfected into human hepatic carcinoma cells （7721 cells） by lipofectin method. The 7721/hscIL- 12 cells which secrete hscIL- 12 stably, were obtained via G418 selection, and in vitro the influence of hscIL- 12 gene transduction on the growth of tumor cells was evaluated by cell cycle analysis. In vivo, genetically engineered 7721 cells （7721/hscIL- 12, 7721/pcDNA） and parental cells were implanted into BALB/c nude mice,respectively. 7721/pcDNA and 7721/hscIL- 12 groups were divided into two sub- groups on day 8: one was administered with hPBL twice, 6 days at interval; the other was given equal volume of PBS. Mice were sacrificed on day 26, and spleens and tumors were taken out for histologic assay. Results. hscIL- 12 produced stably by 7721/hscIL- 12 cells had bioactivity, and it was proved by Western blot, immunocytochemistry, and in situ hybridization. In vitro, compared with 7721 and 7721/pcDNA, the 7721/hscIL- 12 grew much more slowly. FACS assay showed apparent G1 arrest of 7721/hscIL- 12 cells. In animal experiment, on day 8 after inoculation, the tumors of 7721 and 7721/pcDNA group were up to 5～ 7mm,while those of 7721/hscIL- 12 group were 2～ 4mm.When treated with hPBL, the tumor of 7721/hscIL- 12 group disappeared completely. Histologically, the tumors from 7721/hscIL- 12 without hPBL treatment had numerous lymphocyte infiltration, the tumor cells displayed depression looking, atrophy, focal necrosis and apoptosis , whereas the tumors of 7721 and 7721/pcDNA groups grew thrivingly. Conclusion. hscIL- 12 transduced 7721 cells could induced significant antitumor immune response which resulted in tumor regression totally when the hPBL was inoculated, and also hscIL- 12 has certain effects on mice immune system. These findings suggest that hscIL- 12 and hscIL- 12 gene therapy might have promising prospects in clinical application.Keywords：hscIL- 12;antitumor activity;gene transduction;immunity4|13|1Updated：2024-04-10
-
- Abstract：Objective. To explore the changes of three- tone paradigm P300 and observe the improvement of the diagnostic sensitivity of P300 in aging dementia. Methods. Thirty- four patients of aging dementia underwent traditional P300 （2t- P300） and three- tone paradigm auditory P300 （3t- P300）. The results of these two methods were compared. Results. Abnormal P300 was found in 18 of 34 dementia cases （52.9％ ） by 2t- P300, and in 26 of 30 patients who finished the 3t- P300 tests （86.7％ ）. In 2t- P300, 9 of 24 mild cases, 7 of 8 moderate cases and 2 severe cases showed abnormal results. In 3t- P300, 20 of 24 mild cases and all 6 moderate cases showed abnormal results. Abnormal 3t- P300 was found in 13 of 16 cases which showed normal 2t- P300. Besides prolonged and bad- shaped P300, no certain response of P300 to rare non- target stimulus （r- nT） was found in 10 mild cases by 3t- P300. Conclusion. Three- tone paradigm auditory P300 had higher sensitivity in early diagnosis of aging dementia than traditional auditory P300. No P300 response to r- nT in 3t- P300 is an important index in early diagnosis of mild dementia.Keywords：event- related potential;dementia;diagnosis4|13|0Updated：2024-04-10
- Abstract：Objective. The purpose of the study was to build up an animal model of mitochondrial myopathy in order to analyse the pathogenesis of the disease. Methods. The skeletal muscles from Wistar rats treated with germanium dioxide for 24 weeks were analysed by histopathologic and electron- microscopic studies. A quantitative analysis was carried out in mitochondrial DNAs of these samples. The biological function of the model was determined. Results. An animal model of mitochondrial myopathy was built up, in which oxygen free radicals were increased and mitochondrial DNA copies were decreased contrasted with controls. Conclusion. It suggested that environmental toxin may play a role in the pathogenesis of mitochondrial myopathy. The increase of oxygen free radicals is an important link causing the disease.Keywords：mitochondrial myopathy;Wistar rat;animal model4|8|1Updated：2024-04-10
- Abstract：Objective. To establish a PCR- SSP method for discriminating as many HLA- A* 02 alleles, which could easily be introduced into a routine laboratory. Methods. In this study we typed HLA- A* 02 polymorphisms by a sequence- specific primer （SSP） method, which involved round 1 and round 2 PCR reactions to detect 17 HLA- A* 02 alleles （they are HLA- A* 0201- 0217 alleles） covering exon 2 and exon 3. Results. We have found that DNA sample concentration and purity were the most important variables in determining the quality of the results. For identifying correct band size, the size marker used was important. We noticed that different PCR machines performed differently. By this method, we detected 20 HLA- A* 02 positive genomic DNA samples and found 4 kinds of HLA- A* 02 alleles. They were HLA- A* 0201, 0203, 0206 and 0210. Conclusion. The HLA- A* 02 PCR- SSP method was proven to be a reliable and easily applicable typing method. Our results suggest that the SSP described here provides an optimal HLA- A* 02 typing technique that may be useful in selecting donor- recipient pairs in bone marrow transplantation between unrelated individuals.Keywords：DNA typing;PCR- SSP;HLA- A* 02 alleles4|15|1Updated：2024-04-10
- Abstract：Objective. To review our preliminary experience and evaluate our early results of a combined intraoperative iliac angioplasty and stenting with infrainguinal revascularization in multilevel atherosclerotic occlusive disease. Methods. From July 1999 to April 2000, intraoperative iliac angioplasty and stenting combined with simultaneous femoro- popliteal bypass were performed on 12 lower extremities of 10 patients suffering from multilevel atherosclerotic occlusive disease. There were 8 men and 2 women, average 72 years. The indications for procedures included disabling claudication in 3 and rest pain in 7 patients. Results. Eleven iliac angioplasty and stent procedures combined with simultaneous 9 femoro- popliteal bypass and 3 femoro- femoral- popliteal bypass were performed in 12 limbs of 10 patients. Angioplasty and stent placement was technically successful in all patients. One contralateral femoral- popliteal bypass was failure after femorofemoral- popliteal bypass. There were no additional instances of procedural or postoperative morbidity or mortali- ty. Mean follow- up was 5 months （range 1～ 10 months）. During the follow- up period, one femoro- infrapopliteal graft became occluded after 7 months and above- knee amputation was required. The cumulative primary patency rate of stented iliac arteries, femoro- femoral bypass grafts and femoro- popliteal bypass grafts were 100％ （11/11）, 100％ （3/3） and 90.9％ （10/11） in the follow- up period, respectively. The amputation rate was 8.3％ （1/12）. Conclusions. Intraoperative iliac artery PTA and stent placement can be safely and effectively performed simultaneously with infrainguinal revascularization for multilevel atherosclerotic occlusive disease by skilled vascular surgeon, using a portable C arm fluoroscopy in the operating room. Furthermore, iliac artery PTA and stenting was valuable adjunct to distal bypass either to improve inflow and outflow, or to reduce the extent of traditional surgical intervention, and also, any angioplasty and stenting- related complications can be immediately corrected as well.Keywords：atherosclerotic occlusive disease;iliac angioplasty;stenting;femoro- popliteal bypass4|9|0Updated：2024-04-10
-
- Abstract：Objective. To measure the circulating levels of thrombomodulin （TM） and plasminogen activator inhibitor type- I （PAI- I） in women with pregnancy induced hypertension （PIH）. Methods. Blood samples were drawn from 97 pregnant women in their third trimester, grouped as 25 mild PIH,26 moderate PIH,22 severe PIH and 24 normotensive healthy pregnant women for determining levels of TM by ELISA,PAI- I by colorimetric assay methods, and creatinine （Cr） in serum by biochemical method. Results. Circulating levels of TM, PAI- I and TM/Cr ratio increased with increasing severity of PIH. There were no significant differences between mild and normotensive pregnant women. The parameters were significantly changed in the moderate and severe PIH groups. Conclusion. TM and PAI- I may serve as meaningful clinical markers for the assessment of the endothelial damage in PIH, which is very important in evaluating and following the development of PIH.Keywords：pregnancy complication;hypertension;plasminogen activator inhibitor;thrombomodulin4|14|6Updated：2024-04-10
- Abstract：Objective. To explore the relationship between human parvovirus B19 （HPV B19） infection and aplastic anemia （AA） and to investigate the role of HPV B19 in the occurrence of AA. Methods. The presence of HPV B19 DNA was detected in the peripheral blood samples of 60 patients with AA （children 38 and adults 22） by nested polymerase chain reaction （PCR） assay, and 30 healthy persons were selected as control. Results. Sixteen （26.7％ ） of 60 AA cases were HPV B19 DNA positive, while all the samples in the control group were negative for HPV B19 （P = 0.000914）. Among the case group, the positive rates of HPV B19 DNA were 21.4％ （6 / 28）, 30.0％ （3 / 10）, 20.0％ （1 / 5） and 35.3％ （6 / 17） in children acute AA （AAA）, children chronic AA （CAA）, adults AAA and adults CAA patients respectively, which were significantly higher than that in the control group. Furthermore, there was no remarkable difference between children AA and adults AA in the 16 HPV B19 DNA positive patients; neither was there between AAA and CAA. Conclusions. HPV B19 infection is not only correlated with the occurrence of children AAA and CAA, but also with adults AAA and CAA, and might be an important viral cause for AA in humans.Keywords：aplastic anemia;parvovirus B19;nested polymerase chain reaction4|11|1Updated：2024-04-10
- Abstract：Objective. To collect nationwide basic data about cancer related pain. Methods. Sixty cancer patients in each province were randomly selected to participate in this survey. The subjects represented all stages of cancer, tumor sites, and different demographic characteristics. Two self- designed structured questionnaires including reasons, types of pain and pain management were used by patients and physicians respectively. Subjects were asked to report whether he/she had experienced any type of cancer related pain and filled out the equivalent questionnaire. The severity of pain was assessed by using“ visual analogue scale” . Original data input and analysis were using EPI- INFO software package. Results. The result showed that 61.6％ （958/1555） of patients had different types of cancer related pain. Majority of pain （85.1％ ） were caused by advanced cancer. The major reasons （64.4％ ） for poor management or impedimental factors of pain care are due to patient including over- concern on opioid analgesic addiction, reluctance to report pain or refused to use opioid analgesic until at times when pain is intolerable; 26.8％ belonged to physician’s reasons including fear to cause addiction on opioid and lack of knowledge about cancer pain management; 16.2％ are due to lack of different kinds of opioid analgesic for use and 16.1％ belonged to drug regulation. Conclusions. The results showed that majority of patients （61.6％ ） had different types of cancer related pain. In most of patients, cancer pain was relieved when they were treated. The major reason for under- treatment or impeded factors for effective relief of cancer pain was fear of opioid addiction by both medical professionals and patients.Keywords：neoplasm;epidemiology;pain management6|40|3Updated：2024-04-10
- Abstract：In this article, we introduce the system of high throughput screening （HTS）. Its role in new drug study and current development is described. The relationship between research achievements of genome study and new type screening model of new drugs is emphasized. The personal opinions of current problems about HTS study in China are raised.Keywords：high throughput screening;new drug screening;recombinant protein target;automatic analysis4|17|0Updated：2024-04-10
- Abstract：Objective. To investigate the roles of apoptosis in the pulmonary artery remodeling of pulmonary hypertension secondary to hypoxia and illustrate the relative genes expression. Methods. Thirty rats were divided into hypoxia group（ 10％ O2, 8h/d） and normal control group. On the 15th day of hypoxia, pulmonary artery pressure and right ventricular hypertrophy index were measured and pulmonary artery vessels were studied by light microscope. Then terminal deoxynucleotidyl transferase- mediated dUTP nick- end labeling（ TUNEL） technique was used to detect nucleosomal DNA fragmentation of apoptotic cells. In situ hybridization and RT- PCR were used to detect the expression level of bcl- 2 and bax. Results. The pulmonary artery pressure and right ventricular hypertrophy index of hypoxia group were increased significantly, the pulmonary artery wall of hypoxic group become incrassate than control group. Apoptotic cells can be found in lung with hypoxia or without hypoxia. Compared with control group, apoptotic index of hypoxic group decreased significantly. Through the methods of in situ hybridization and RT- PCR, we found the expression of bcl- 2 increased whereas bax decreased significantly in the hypoxic group. Conclusion. The alternation in bcl- 2 and bax expression induced by hypoxia play an important role in the pulmonary artery remodeling which is the main pathologic change of pulmonary hypertension secondary to hypoxia.Keywords：pulmonary hypertension;pulmonary artery remodeling;bcl- 24|18|11Updated：2024-04-10
-
-
- © 2024 Chinese Academy of Medical Sciences 京ICP备09064830号-19
京公网安备11010802024621 - It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
- Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.
0