Latest Issue
Volume 0 ， Issue 2 ， 2002
- Abstract：Objective.To search for genomic DNA sequence of the augmenter of liver regeneration（ALR）of rat.Methods.Polymerase chain reaction（PCR）with specific primers was used to amp lify the sequence from the rat genome.Results.A piece of genomic DNA sequence and a p iece of pseudogene of rat ALR were identified.The lengths of the gene and pseudogene are 1508bp and 442bp,respectively.The ALR gene of rat includes 3exons and 2introns.The 442bp DNA sequence may represent a pseudogene or a ALR-related peptide.Predicted amino acid sequence analysis showed that there were 14different amino acid residues between the gene and pseu do-gene.ALR-related peptide is 84amin o acid residues in length and relates closely to ALR protein.Conclusion.There might be a multigene family of A LR in rat.Keywords：liver regeneration;polymerase chain reaction;gene;pseudogene4|10|0Updated：2024-04-10
- Abstract：Objective.To study the therapeutic T cell vaccine for the treatment of chronic hepatitis B by improving the cellular immunization of HBsAg vaccine with the coexpression of the preS1（1-42）and the Core（1-144）anti-gen of HBV in E.coli.Methods.The genes of HBcAg （1-144）and preS1（1-42）were amplified and fused by PCR.This fused gene was inserted in the prokaryotic expression vector pET-11d and expre ssed in E.coli.Results.It was showed by SDS-PAGE that the pr otein molecular weight of the coexpr ession product was about 20kD,20%of all bacteria prote in.The monoclonal antibodies again st core and preS1antibody could re-act with this fused protein by Western-blot technique respectively.The fused gene was verified by sequencin g.Under the immune electron microscop y,this fused protein is typical particles of HBcAg but in an aggregated form.Conclusion.The results might aid for studying T c ell immunotherapeutic vaccine for c hronic hepatitis B.Keywords：HBV preS1antigen;HBcAg;coexpression of fused gene3|14|2Updated：2024-04-10
- Abstract：Objective.To identify the A3243G mutation of mi tochondrial（mt ）DNA in patients with latent autoimmu ne diabetes mellitus in adults（LADA）of Han nationality in the northeast a rea of China.Methods.Seventy-nine diabetics of Han natio nality,whose families have resided in the northeast area of China for more than 3generations,we re divided into 3groups:Group 1（22cases of type 2diabetes with mater-nal inheritance history）,Group 2（34cases of LADA）,Group 3（23cases of type 1diabetes in adolesc ents）.The A3243G of mt DNA was detected in th ese 79subjects with the method of PCR-RFLP.Results.None of the 79diabetics studied was p ositively identified for the A3243G mutation of mt DNA.Conclusion.The A3243G mutation of mt DNA might not be related to the onset of LADA in dia betic popula-tion of Han nationality in northeast area of China and there might not be close relationship between A3243G mut a-tion of mt DNA and autoimmunity.Keywords：mitochondrial gene;autoimmune dia betes mellitus;autoimmune4|14|0Updated：2024-04-10
- Abstract：Objective.To assess patients’ knowledge,attitudes,and concerns regarding anesthetic management.Method.A survey of 55items was developed and administered preoperatively to 500patients including 190men and 310women in our hospital.Patients were interviewed on their know ledge of the role of anesthesiolo-gists,their preferences regarding anesthetic management,and also their concernaboutpotential anesthetic complica-tions.Results.Patients’ perceptions ofanesthesiologists’ training androle have reacheda certainlevel.Mostsignifi-cant preoperative concerns regardi ng the anesthesiologists focused on experience,qualifications,and pr esence or absence during the anesthesia.Patients’ concerns also included the possibility of not being waken up following anesthesia,experiencing postoperative pain,and becoming paralyzed.The variations of concerns depended partial-ly on patients’ sex,type of anesthesia,and propose d surgical procedure,partially on t heir education and living environments.Conclusion.It is suggested that anesthesiologists address significant patient con cerns during the preopera-tive visit to enhance their effectiv eness in patient care.Efforts to educate the public on the anesthesiologists’ role in preoperative care would improve p atients’ confidence.Keywords：surgical operation;patients;anesthesia4|17|0Updated：2024-04-10
- Abstract：Objective.To construct recombinant BCG again st leptospirosis.Methods.We amplified the entire open readin g frame of the OmpL1gene from the genome of the leptospire serovar Lai strain 017.Two recombin ant plasmids pBQ1and pBQ2were constructed by oriented ligation based on the E.coli-BCG shuttle plasmids pMV261and pMV361respectively.The recombinant plasmids were transformed into BCG by electroporation.The rBCGs bearing pBQ1and pBQ2were induced by high temperature of 45℃.Results.The expressed product,a 35kD prote in was detected by SDS-PAGE.The resu lt indicates that pBQ1and pBQ2can express OmpL1in rBCG.Conclusion.The technical methods in this study may help detect the immunogenicity a nd immunoprotection of OmpL1and develop more safe,highl y effective rBCG bearing leptospira l antigen with long-lasting protection.Keywords：Leptospira interrogans serovar Lai;recombinant BCG;OmpL1gene3|36|3Updated：2024-04-10
- Abstract：Objective.To summarize the experience of minim ally invasive treatment in 520patie nts with intracranial aneurysms on a retrospective study.Methods.The measures used in the treatment of520patients were reviewed in terms o f timing of surgery,induced-hypotensive anesthesia,b rain protection combined with temporal occlusion of the feeding artery,exter-nal drainage of CSF,dynamic monitoring of intracranial pressure,blood flow velocity,serum osmolality and CT scanning,anti-vasospasm therapy a s well as selected interventional en dovascular embolization of aneurysms.Results.Of the 520patients,485were treated with either direct clipping or endov ascular embolization and35patients were treated non-surgic ally.In 449patients undergoing dir ect clipping and 36undergoing endov ascu-lar embolization,intraoperative r upture of aneurysm occurred in 27（6.0%）and 0%,respectively.Death oc-curred in 13（2.6%）,hemiplegia in 8（1.6%）,and vegetative state in 2（0.4%）.The operative mortality of direct clipping was 3.8%in 210patie nts before 1990and 1.8%in 275patien ts after 1990（36patients undergo-ing endovascular embolization,the operative mortality was 0%）.Conclusion.The outcome of patients with intracranial aneurysms can be markedly impr oved and the opera-tive mortality can be lowered by mini mally invasive treatment.Keywords：aneurysm;spontaneous subarachnoid hemorrhage;minimally invasive neurosurgery4|12|2Updated：2024-04-10
- Abstract：Objective.To explore the expression characteristic of fibronectin gene in hypertr ophic scars and diabetic ul-cer tissues.Methods.The biopsies from normal skins,hypertrophic scars and diabetic foot ulc ers were taken.The tech-nique of quantitative polymerase ch ain reaction（PCR）was used to evaluate the gene express ion of fibronectin in the above biopsies.Results.Fibronectin gene expression was enhanced in hypertrophic scars and decreased in diabetic foot ul-cers compared with that in normal ski ns.Quantitative comparison showed about 2-fold increase of fibronectin mR-NA level in hypertrophic scars and ab out 3-fold decrease of fibronectin mRNA level in diabetic ulcers as com-pared with that in normal skins.Conclusions.Fibronectin gene expression is influenced by the tissue environment.Di fferent expression and synthesis of fibronectin may cause d ifferent outcomes in wound healing.Keywords：wound healing;fibronectin;gene;c hronic ulcer;scar5|19|3Updated：2024-04-10
- Abstract：Objective.It was suggested that coronary stent design and coating may affect stent performance and hence induce varying degrees of thrombogenesis and neointimal hyperplasia.T he purpose of this study is to compare the6-month follow-up results between o ld and new Multilink stents with the method of intravascular ultrasound（IVUS）imaging.Methods.We have performed old（n =40）and new（n =35）Multilink stent implantations on 75patients with coronary artery disease.Coron ary angiography was performed before,immediately after,and 6months after the in-stent procedure respectively.Six-month follow-up IVUS imaging was performed and analyzed off-line.Results.Minimal lumen cross sectional area（CSA）of new Multilink stents was signific antly larger than that of old Multilink stents（P=0.0053）.Mean stent lumen area of new Multili nk stents was significantly larger than that of old Multilink stents（P=0.040）.Similarly,minimal lumen diameter（MLD）of new Multilink stents was larger than that of old Mul tilink stents（P=0.011）.Old Multilink stents had a higher percentage of plaque area than new Multilink stent s.Conclusion.The new Multilink stent is obviously superior to old Multilink stents,in particular,in the stent MLD and lumen CSA---major determinants of the restenosis.Keywords：stents;coronary artery disease;in travascular ultrasound imaging4|3|0Updated：2024-04-10
- Abstract：Objective.To investigate the frequency of p16a nd p15gene methylation in multiple myeloma （MM）,and its relationship with bone marrow ce ll apoptosis and clinical outcome.Methods.Twenty-two patients with MM were stu died to detect p16and p15gene methylation.Methyla-tion-specific polymerase chain rea ction（MSP）was used to detect gene methylation,and terminal trans-ferase-mediated dUTP nick end-labeling（TUNEL）was used to detect cell apoptosis.Results.p16and /or p15gene methylatoin was d etected in 10of 22patients（45.4%）.There were 3pa-tients with p16gene methylation,9p atients with p15gene methylation,a nd 2patients with both genes methyla-tion.The incidence of methylation o f p15gene was higher than that of p16g ene（P<0.05）.The patients with p16and /or p15gene methylation had a delayed cell apoptosis,poor respon se to chemotherapy,and a short over-all survival（OS）.Conclusion.The methylation of p16and /or p15gen e plays a key role in MM apoptosis path ogenesis.The patients with both p16and p15gene me thylation had a poor prognosis.Keywords：multiple myeloma;p16gene;p15gene;methylation2|10|2Updated：2024-04-10
- Abstract：Objective.To determine the apoptotic and proliferative activities in various ovarian epithelial tumors.Methods.Formalin-fixed,paraffin-embedded tissues of 86ovarian epithelial tu mors,including 52adenocarcino-mas,23borderline tumors and 11cystadenomas,were retrieved.Apoptoti c（AI ）and proliferative（PI ）index were estimated using the monoclonal antibodies:M30,Ki-67and Ki-S1in t hese tumors.Quantitative assess-ment of AI and PI was estimated by calc ulating the percentage of positive c ells among no less than 1000tumor cells.Results.Statistically significant differe nce in AI was found between benign and borderline tumors or carcino-mas（P=0.028,0.001,respectively）.Significant differences in PI,as a ssessed by both Ki-67and topo IIα,were demonstrated between carcinom as and benign or borderline tumors（both P<0.001）.Benign tumors had both low PI and AI;borderline tumors had lower PI but higher AI,while aden ocarcinomas had both high prolifera-tive and high apoptotic rates.Among borderline tumors,serous tumors had significantly lower AI and higher PI than mucinous ones.Conclusions.The results suggest that apoptotic a nd proliferative activities play im portant roles in the pathogene-sis and development of ovarian borderline and malignant tumors.The high apoptotic rate in borderline tumor m ay explain its relatively indolent beh avior while the high proliferative r ate in carcinomas tends to explain its aggres-sive behavior.Keywords：Ki-67;M30;topoisomerase IIα;ovarian epithelial tumor4|20|0Updated：2024-04-10
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- Abstract：Objective.To investigate the effect of peroxis ome proliferator-activated recept ors（PPARs ）activators on plasminogen activator inhibitor ty pe-1（PAI-1）expression in human umbilical vein e ndothelial cells and the possi-ble mechanism.Methods.Human umbilical vein endothelial ce lls（HUVECs ）were obtained from normal fetus,and cul-tured conventionally.Then the HUVECs were exposed to test agents（linolenic acid,linoleic acid,oleic acid,stearic acid and prostaglandin J 2 respectively）in varying concentrations with fresh media.RT -PCR and ELISA were applied to determine the expression of PPARs and PAI-1in HUVECs.Results.PPARα,PPARδand PPARγmRNA were detected by using RT-PCR in HUVECs.Treatment of HUVECs with PPARαand PPARγactivators---linolenic acid,linoleic acid,oleic acid and prostaglandin J 2 respectively,but not with stearic a cid could augment PAI-I mRNA expression and protein secretion in a concentration-dependent manner.However,the mRNA expressions of 3subclasses of PPAR with their activators in HUVECs were not changed compared w ith controls.Conclusion.HUVECs express PPARs.PPARs activators may increase PAI-1expression in ECs,but the underlying mechanism remains uncle ar.Although PPARs expression was not enhanced after stimulated by their activators in ECs,the role of functionally active PPARs in regulating PA I-1expression in ECs needs to be further investigated by using transient gen e transfection assay.Keywords：peroxisome proliferator-activate d receptors;plasminogen activator inhibitor type-1;expression;endothelial cells4|13|4Updated：2024-04-10
- Abstract：Objective.To analyze the clinical characteris tics of peripartum cardiomyopathy a nd to evaluate the different factors that influence the prognosis of the peripartum cardiomyopathy.Method.A retrospective review was undertak en on records of women who were diagnosed with peripartum cardiomyopathy at Peking Union Medi cal College Hospital between Jan.1983and May 1999.Results.During the research period,only 16p regnant women were documented as peripartum cardiomyopa-thy.Some of the women undertook ultr asonic cardiographic（UCG）examination that showed decreased s ystolic function.Seven women were complica ted with pregnancy induced hyperten sion.Three died of disseminated intravascular coagulation,embolism and cardiogenic shock respectively.Conclusion.Early diagnosis of the peripartum ca rdiomyopathy is extremely important.The UCG can pro-vide helpful information on disease progression or regression.Keywords：peripartum cardiomyopathy;heart failure;peripartum4|13|0Updated：2024-04-10
- Abstract：Objective.To investigate the serum sE-cadherin level in patients with endometriosis and the alterations of that level in healthy control during the menstrual cycle.Methods.Thirty-two patients with endometriosis and 30healthy women were tested for serum sE-cadherin levels by enzyme-linked immunosorb ent assay.Results.The serum sE-cadherin levels in healthy control did not vary throughout t he menstrual cycle,which were lower than those in patients with endometriosis.Conclusions.E-cadherin might be involved in endometrial shedding during menstruati on in endometriosis pa-tients.The serum sE-cadherin assay might be helpful as a serum marker for the diagnosis and management of endometriosis.Keywords：endometriosis;cadherin2|22|8Updated：2024-04-10
- Abstract：To get a better understanding of the l ocation,pathophysiology,etiology and prognosis of multiple malignan t tumors（MPMT ）,we evaluated the medical records of22patients with MPMT.Our results su ggested that radiothera-py and chemotherapy might play an imp ortant role in the pathogenesis of MP MT and follow-up is important in dete ct-ing a secondary primary malignant tu mor（PMT ）at an early stage.Surgical removal o f tumors is the first-choice therapy for MPMT.Keywords：multiple malignant tumor;clinicop athological analysis6|6|3Updated：2024-04-10
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