Latest Issue
Volume ， Issue 2 ， 2003
- Abstract：Objective. To investigate the properties of voltage-gated sodium （Na+） channels in developing auditory neurons during early postnatal stages in the mammalian central nervous system.Methods. Using the whole-cell voltage-clamp technique, we have studied changes in the electrophysi-ological properties of Na+ channels in the principal neurons of the medial nucleus of the trapezoid body （MNTB）.Results. We found that MNTB neurons already express functional Na+ channels at postnatal day 1 （P1）, and that channel density begins to increase at P5 when the neurons receive synaptic innervation and reach its maximum （3 fold） at P11 when functional hearing onsets. These changes were paralleled by an age-dependent acceleration in both inactivation and recovery from inactivation. In contrast, there was very little alteration in the voltage-dependence of inactivation.Conclusion. These profound changes in the properties of voltage-gated Na+ channels may increase the excitability of MNTB neurons and enhance their phase-locking fidelity and capacity during high-frequency synaptic transmission.4|6|0Updated：2024-04-10
- Abstract：<正> 5-8 June, 2003-41st Nordic Lung CongressReykjavik, IcelandFor further information:Congress Secretariat:CongressReykjavik, c/o Lara B.Ptursdttir,Engjateigi 5,105 Reykjavik, Iceland.4|1|0Updated：2024-04-10
- Abstract：Purpose. To evaluate and compare the effects of heart rate on conduction velocity in the cavotricus-pid isthmus （CTI） and septum in patients with and without typical atrial flutter （AF） using electro-anatomic mapping （EAM） of the right atrium （RA）.Methods. Ten patients（age 53+10 yrs,7M/3F）with AF and 13 patients （age 51+11 yrs, 5M/8F） with atrio-ventricular nodal reentrant tachycardia （AVNRT） underwent conventional electrophysionogical study, electro -anatomic mapping and radiofrequency ablation. Using EAMs obtained during coronary sinus pacing at pacing cycle length （PCL） 600 ms, 400 ms, and 300 ms, we evaluated conduction velocities in the CTI and septum of RA in 10 patients with AF and compared EAMs to 13 patients with AVNRT to determine whether the conduction slowing required to maintain AFL was related to changes in volume alone or altered RA electrophysiology.Results. Conduction velocities in CTI and septum were significantly slower at all PCL when AF was compared to AVNRT（P<0.05）. Additionally, in the AF group, septal conduction velocities were slower at PCL 600 ms and 400 ms, but not at 300 ms compared to CTI （P<0.05）. In AF, during PCL 300. conduction in CTI slowed significantly compared to PCL 600 and 400 ms such that there was no difference between CTI and septum at PCL 300.Conclusions. There is slower conduction in the septum compared to the CTI in all patients. However, in patients with AF, there is significant slowing of conduction in the CTI and septum as well as decremen-tal rate-dependent slowing of conduction in the CTI. These findings indicate that in addition to RA enlargement, changes in atrial electrophysiology distinguish AF patients from patients with AVNRT.4|8|0Updated：2024-04-10
- Abstract：Objective. To investigate the role of matrix metalloproteinase-7 （MMP-7） expression in caricinogenesis and progression of gastric cancer.Methods. We studied MMP-7 expression and microvessel density （MVD） in adjacent mucosa and primary foci of 113 cases of gastric cancer by streptavidin -biotin -immunoperoxidase method using anti -MMP-7 and anti-CD34 antibodies. MMP-7 expression and mean MVD were compared with clinicopatholog-ical features of gastric cancer, with the relationship between MMP-7 expression and MVD concerned in gastric cancer.Results. MMP-7 showed positive expression in adjacent mucosa of gastric cancer （29.20%, 33/113）, less than that in gastric cancer （69.03%, 78/113）. MMP-7 expression in primary foci of gastric cancer was positively correlated with tumor size, invasive depth, metastasis and TNM staging （P<0.05）, but not with differentiation or growth pattern of gastric cancer （P>0.05）. Positive correlation of mean MVD with tumor size, invasive depth, metastasis and TNM staging was found （P<0.05）, despite no relationship between mean MVD and differentiation of gastric cancer （P>0.05）. Mean MVD was dependent on MMP-7 expression in gastric cancer （P<0.05）.Conclusion. Up-regulated expression of MMP-7 played an important role in carcinogenesis and progression by participating in growth, invasion, metastasis and angiogenesis of gastric cancer. MMP-7 expression could be regarded as an effective and objective marker to reflect the biological behaviors of gastric cancer.4|16|1Updated：2024-04-10
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THE ASSOCIATION OF THE EXPRESSION OF MTA1, NM23H1 WITH THE INVASION, METASTASIS OF OVARIAN CARCINOMA
Abstract：Objective. To understanding the molecular mechanisms in invasion and metastasis of the ovarian carcinoma, we investigate a novel candidate metastasis-associated gene （ MTA1） and nm23Hl mRNA expression and mutation in ovarian carcinoma.Methods. Twenty primary ovarian carcinoma specimens, 20 corresponding lymph nodes and 8 normal ovarian was examined for mRNA expression and mutation of MTA1 and nm23Hl genes by reverse-transcription ploymerase chain reaction （RT-PCR） and RT-PCR-SSCP analysis. The level of the expression was determined by the relative optic desity （ROD） of the PCR products.Results. The frequency of MAT1 overexpression was 100% （7/7） in primary ovarian carcinoma with metastasis but only 38.5% （5/13） in those without metastasis （P=0.0103）. Overexpression of MAT1 was observed in 87.5%（6/7） of lymph nodes with metastasis but only 23%（3/13） of lymph nodes without metastasis （P=0.0118）. In contrast with MAT1, low expression of nm23Hl mRNA was seen in 7 of 7 o-varian carcinoma with metastasis but only in 4 of 13（30%） of those without metastassis （P=0.0043）. Low nm23Hl expression was also seen in 7 of 7 lymph nodes with metastasis but only in 5 of 13 （38.5%） nonmetastatic lymph nodes （P=0.0102）. The ROD ratio of MAT1 to nm23Hl increased with the development of metastasis. No mutation of MAT1 and nm23Hl genes was found by SSCP analysis.Conclusion. The mRNA expression of MTA1 and nm23Hl is positively and negatively correlated with lymph node metastasis, respectively. Expression abnormalities but not mutation of the two genes are frequent events related to lymph node metastasis of ovarian cancer.4|26|39Updated：2024-04-10 - Abstract：<正> The present study analyzed the DNA content（ratio of heterploid,RH）,s-phase cell（SPC）per-centage,the proliferating cell nuclear antigen（PC-NA） labelling index and area of nucleolar organizerregion （AgNOR） in 57 patients with laryngeal can-cer. The results were shown in Table 1.4|4|1Updated：2024-04-10
- Abstract：Objective. To evaluate the surgery in simple ovarian cysts.Methods. From Jan.1994 to Dec.1999, 221 women with simple ovarian cysts were admitted into our hospital. The diameter of cysts was <5 cm in 76 cases, and was>5 cm in 145 cases ultrasonically. One hundred and eighty-four patients underwent laparosocopy, and thirty-seven underwent laparotomy.Results. Histological findings showed no malignancy in this series. Simple cysts, paraovarian cysts and corpus luteum were found histologically in 90.8% of cases with cysts <5 cm, and 60% of those with cysts >1 cm respectively, their difference was significant （x2=37.13, P<0.001）. The simple cysts, paraovarian cysts and corpus luteum cysts were found in 81.5% of postmenopausal women and 84.0% of pre-menopausal women.Conclusion. Patients with cysts >1 cm are indicated for surgical procedures, while a period of fol-lowup is acceptable for patients with cysts <7 cm, and surgery is advisable if the cyst is persistent during followup. Postmenopausal women with cysts should have operations.4|20|0Updated：2024-04-10
- Abstract：Objective. To study the whole body bone mineral changes in normal subjects.Methods. Bone mineral was measured by dual X-ray absorptiometry （DXA） in 292 normal subjects aged 1079 years, including 152 females and 140 males. They were divided into age-groups by decade.Results. In males, the ages with peak value of total bone mineral content （TBMC）, total bone mineral density （TBMD）, anteroposterior spine BMD（L2-4 BMD） were in the 30-39,20-39, 20-29 age-groups respectively. In females, they were all in the 30-39 age-group. Peak values of TBMC, TBMD, L2-4 BMD were higher in males than those in females. Loss of bone mineral for females was more pronounced than that for males. Loss of L2-4 BMD was more pronounced than TBMD.Conclusion. This study provides the normal bone mineral data of whole body for males and females, and the characteristic of changes.4|10|0Updated：2024-04-10
- Abstract：Objective. To investigate the effect of peritoneal dialysis on fluid balance and outcome in children who receive cardiac operation.Methods. From July to Dec. 2000, 12（2.08%） patients of 576 consecutive children who underwent heart operation required peritoneal dialysis because of acute renal failure. The mean age of these 12 patients was （2.9-2.0） years （range, 5 months-7 years） and the mean body weight was （12+3） kg （range, 7.4-18.5 kg ）.Results. The interval between the operation and the initiation of peritoneal dialysis was （21.2+11.4） hours （4.4-42 hours）. The duration of peritoneal dialysis was （6.3+4.8） days （0.47-15 days）. Mortality in these 12 patients was 25%. Fluid removed by peritoneal dialysis was（34.7+17.8） ml . kg-1 . day-1. Asymop-tomatic hypokalemia, thrombocytopenia and hyperglycemia were frequent complications, which were easily managed. Hemodynamics and pulmonary function improved during the study period.Conclusion. The early initiation of peritoneal dialysis is an effective and safe method to treat acute renal failure after cardiac operation in children.4|10|1Updated：2024-04-10
- Abstract：Objective. To investigate the changes and influencing factors of early postoperative pulmonary function of thoracotomy.Methods. Pre-and early postoperative pulmonary function was studied in 64 consecutive cases with optimal thoracotomy. Pain assessment was done before pulmonary function test, and the chief complaints of patients were recorded after the procedure. The changing curves of pulmonary function were done and the differences associated with groups, surgical styles, pain assessment, epidural analgesia, chief complaint and preoperative conditions were analyzed.Results. Pulmonary function was severely lowered to about 40% of the base line on the first day, and it was rehabilitated to about 60% of the base line on the eighth day. There was a greater gradient on the recovery curve on the 3rd and 4th days. Epidural analgesia was able to improve pain relaxation and pulmonary function in some degree. Single-factor analysis showed that postoperative pain, postoperative day and surgical style were the significant influencing factors for early postoperative pulmonary function. By multiple-factor analysis, preoperative pulmonary function, age and postoperative pain were the main factors, while surgical style had only weak effect on it.Conclusions. Early postoperative pulmonary function is severely impaired by thoracotomy. It rehabilitate gradually with time. Improvement of preoperative pulmonary function, reducing surgical procedure injuries, especially injury to respiratory muscle system, and enough postoperative pain relief are the most important means that would reduce pulmonary function impairment and consequently reduce postoperative pulmonary complications.4|11|5Updated：2024-04-10
- Abstract：Objective. Report the results of interventional or semi-interventional techniques for 173 patients with Budd-Chiari syndrome.Method. This group included 120 males and 53 females. The pathologic lesions composed of localized complete occlusion of inferior vena cava （IVC） （78）, IVC stenosis （49）, IVC membrane with a hole （37）, membrane of hepatic vein （HV） （3）, IVC thrombosis （4）, IVC membrane with thrombosis （2） and IVC lesion with occlusion of HV （32）. Treatment methods included that I: Percutaneous transinferior vena cava angioplasty （PTA） （76）; II: IVC PTA with stent （59）; III: Percutaneous transhepatic vein recanalization （3）; IV: IVC thrombolysis through a catheter （4）; V; Combined transcardiac and trans-femoral venous membranotomy and balloon dilation （22）; VI: V and stent （17）; VII; Stenting during radical surgery （3）; VIII: Additional operation after intervention （23）.Results. The immediate technique success rate for intervention was 90.1%, for the semi-intervention was 100%. The IVC pressure was reduced from 3 to 29 cmH20. Complications occurred in 8 cases. The death rate was 2.9%. A follow-up study showed the recurrence rates were 14.5% in IVC PTA group, 1.7% in IVC PTA with stent, 18.2% in combined technique without stent and no recurrence was found in other groups.Conclusion. The PTA is the first choice for localized lesions. When elastic recoil occurs, immediate stenting is suggested. The semi-interventional approach is advised for PTA failure and more complicated cases. For those with both IVC lesion and occlusion of HV, the additional operation is needed after IVC intervention.4|32|10Updated：2024-04-10
- Abstract：Objective. To describe our surgical experience on rupture of abdominal aortic aneurysm .Methods. Two cases of ruptured aortic aneurysms with severe complication were analyzed. Aorta reconstruction procedures were performed using bifurcated e-PTFE grafts during emergency operation. Diagnosis, preoperadve resuscitation, emergency surgical intervention, and postoperative complications of these patients were summarized and discussed.Results. Rupture of aortic aneurysm in both patients presented as a huge retroperitoneum haematoma by computed tomography scan. They were successfully saved by prompt body fluid compensation, emergency procedure, intraoperative resuscitation, and postoperative intensive care.Conclusions. Correct diagnosis, prompt surgical management, immediate intraoperative proximal aorta clamping during procedure, and effective management of postoperative complications were the key points to successful treatment of ruptured aortic aneurysm.4|18|1Updated：2024-04-10
- Abstract：Objective. To clarify the frequency, presentation, associated factors, treatment and outcome of hy-ponatremia after transsphenoidal surgery of pituitary adenomas.Methods. Retrospectively reviewed the database of 183 patients who underwent transsphenoidal surgery of pituitary adenomas between January 1999 and June 2000 in our department.Results. 38.8% （71/183） had postoperative hyponatremia. Among them, 59.2% （42/71） appeared on the 4th to 7th day postoperatively. 59.2% （42/71） presented with nausea, vomiting, headache, dizziness, confusion and weakness. Hyponatremia was related to age, tumor size and adenoma type, but not related to sex and degree of resection. Treatment consisted of salt replacement and mild fluid restriction in 4 patients and salt and fluid replacement in 67 patients. Hyponatremia resolved within 16 days in all the patients.Conclusions. Hyponatremia often appeared about 7 days after transsphenoidal surgery of pituitary adenomas, especially in elderly and patients with macroadenomas and huge pituitary adenomas. The principle of treatment was salt and fluid replacement.4|20|5Updated：2024-04-10
- Abstract：Objectives. To detect telomerase activity in patients with mycosis fungoides （MF） and to study the role of telomerase in the tumorigenesis of MF.Methods. The technique of PCR-ELISA was employed to detect telomerase activity in 35 patients with various stages of MF.Results. 92.3% tumor stage of MF, 78.6% plaque stage of MF and 75.0% patch stage of MF had positive telomerase activity. The control samples had no telomerase activity. Telomerase activity in tumor stage of MF was significantly higher than that in plaque stage, while the latter was higher than that in patch stage. Telomerase activity was correlated with the stage of MF.Conclusion. High level of telomerase activity frequently occurred in patients with MF, suggesting that telomerase might play an important role in the tumorigenesis of MF and is a useful marker for the diagnosis of MF possibly.4|12|1Updated：2024-04-10
- Abstract：Objective. To study the characteristics of changes of LDH enzyme patterns of mice under slight hypoxia.Methods. Mice treated with artificial hypoxia, various tissues were made for the test of LDH enzymatic activity by the specific staining technique. LDH （1 -5） relative percentage enzymatic activity （RPEA） were measured with CS-910 dual-wavelength thin layer chromatography scanner.Results. The RPEA of LDH isozymes of various tissues after slight hypoxia shifted to the isozymes LDH1 and LDH2, whose principal subunits are H subunits, and the RPEA of LDH,（H4）, LDH2（H3M） increased, while RPEA of LDH5（M4） in various tissues decreased prominently except the cardiac muscle, and that of LDH4（HM3） decreased as well. After polyacrylamide gel electrophoresis （PAGE） of the hypoxia treated cardiac muscle specimen was made, activity subbands originated regularly in the isozyme patterns of LDH, with the regularity of LDH1 （0 subband）, LDH2 （0-1 subbands）, LDH3 （0-2 subbands）, LDH4 （1-3 subbands）, LDH5 （2-4 subbands）. After adding appropriate amount of NAD+ to the hypoxia treated cardiac muscle specimen, PAGE showed the subbands of four isozymes （LDH2-LDH5） reduced or even totally disappeared in the isozyme patterns.Conclusions. The negative feedback regulation of coenzymization and decoenzymization of LDH isozymes is one of the mouse stress responses to slight hypoxia.4|13|0Updated：2024-04-10
- Abstract：<正> In this study,we investigated preoperatlve diag-nostic value of telomerase activity for breast cancerand the correlation between biological characteristicsof breast tumor and telomerase activity.4|4|0Updated：2024-04-10
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