Latest Issue
Volume 0 ， Issue 3 ， 2004
- Abstract：To clone and identify the proteins involved in regulating the transcription of hTERT and study the role of genes in both hTERT transcription and telomerase activity. Methods The full cDNA of COUP-TFII was cloned from HeLa cDNA library by hTERT promoter-based yeast one-hybrid assay and then in-frame inserted into His-tag fusion expression vector pEK318. The His-tag COUP-TFII fusion proteins were purified by Ni-NTA chromatography. The interaction of COUP-TFII with hTERT promoter in vitro was identified by electrophoretic mobility shift assay and Footprint. The role of COUP-TFII in both hTERT transcription and telomerase activity were probed through Luciferase reporter assay, Northern blot, and TRAP-PCR ELISA. Results COUP-TFII could firmly bind to the downstream E-box and the other two binding sites in hTERT promoter. Luciferase reporter assay indicated COUP-TFII could suppress hTERT promoter activity and stable introduction of COUP-TFII into HeLa cells also decreased both endogenous hTERT transcription and telomerase activity. Conclusion The human COUP-TFII can firmly bind to hTERT promoter, and inhibit telomerase activity through decreasing hTERT transcription. It will greatly facilitate understanding of telomerase regulation in normal and cancer cellsKeywords：COUP-TFII;hTERT;telomerase;yeast one-hybrid assay;transcription regulation4|28|4Updated：2024-04-10
- Abstract：To investigate whether the polymorphisms of β2-adrenergic receptor （β2-AR） at position 16, 27, 164 are asso-ciated with asthma in Northern Chinese subjects. Methods Genomic DNA was collected from unrelated Northern Chinese population of Han ethnicity, including 125 un-related asthmatic individuals and 96 healthy controls. β2-AR genes at position 16, 27, 164 were amplified by using restriction fragment length polymorphism （RFLP） and allelic specific polymerase chain reaction methods. All asthmatics had their serum IgE （total and specific） antibody or skin-prick test measured, bronchial reactivity to methacholine （Mch） and bronchial rever-sibility by β2-agonist evaluated. Results （1） The frequency of Gly 16 homozygous was significantly higher in the asthmatic group than that in healthy controls （22.4% vs. 8.3%, P < 0.05）, OR was 2.9 with 95% CI 1.26-6.78. The proportion of Gly 16 allele was also higher in asthmatics than that in control （0.46 vs. 0.36, P < 0.05）; Gly16 homozygous was not independently associated with asthma pathogenesis （P = 0.21, OR 0.42 with 95% CI 0.11-1.61）. （2） Of 51 night attack patients, 18 carrying Gly16 homozygosity, if compared with 10 of 74 nonnocturnal asthmatics carrying this genotype, there was significant difference between these two groups （35.3% vs. 13.5%, P < 0.01）. （3） The average dose of PD20-Mch was significantly lower in patients carrying Gln 27 homozygous than those carrying homozygous Glu 27 and Gln/Glu 27 heterozygous （0.2 ± 0.3, 1.6 ± 0.8, and 2.1 ± 3.0 μmol/L,P < 0.05）. Conclusion β2-AR gene polymorphisms might confer susceptibility to asthma in Chinese Northern patients. β2-AR gene, coordinated with other candidate loci, plays a role in the development of asthma.Keywords：adrenergic receptor;polymorphism;asthma4|20|1Updated：2024-04-10
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EXPRESSION OF mRNA FOR MEMBRANE-TYPE 1, 2, AND 3 MATRIX METALLOPROTEINASES IN HUMAN LARYNGEAL CANCER
Abstract：To investigate correlation of expressions of membrane-type 1, 2, and 3 matrix metalloproteinases （MT1, MT2, and MT3-MMP） to the invasion and metastases in laryngeal cancer. Methods Reverse transcription-polymerase chain reaction （RT-PCR） was used to examine the mRNA level of MT1, MT2, and MT3-MMP in 24 patients with laryngeal cancer. The relationships of these three MT-MMP expressions to clinico-pathology were analyzed by statistics. Results The expressions of MT1, MT2, and MT3-MMP were significantly higher in laryngeal cancer tissues than those in para-tumorous tissues （P < 0.01） and had a close relationship with invasive depth （P < 0.05）. But no significantly different expressions of these three MT-MMPs were found in different primary location and different histological grade of laryngeal cancer （P > 0.05）. The expression of MT1-MMP was obviously higher in patients with metastatic lymph nodes than that in patients without metastatic lymph nodes （P < 0.05）. Conclusion MT1, MT2, and MT3-MMP play an important role in the progression of laryngeal cancer, and MT1-MMP may serve as a reliable marker in estimating invasive and metastatic potency of laryngeal cancer. Suppressing expressions of MT1, MT2, and MT3-MMP early may inhibit the invasion and metastases of laryngeal cancer.Keywords：laryngeal cancer;membrane type matrix metalloproteinases;polymerase chain reaction4|8|4Updated：2024-04-10 - Abstract：To evaluate the value of 16-slice spiral CT in the demonstration of coronary artery and in the diagnose of coronary artery stenosis. Methods Plain and enhanced CT scans were performed with a 16-slice CT scanner （Sensation 16, Siemens, Germany） in 230 patients with suspected coronary heart disease （CHD）. Parameters of the plain scan were: 120 kV, 133 mA, slice col-limation 16 mm×1.5 mm, rotation time 0.42 seconds, increment 1.5 mm, and slice width 3 mm. Parameters of the enhanced scan were: 120 kV, 500 mA, slice collimation 16 mm×0.75 mm, rotation time 0.42 seconds, increment 0.5 mm, and slice width 1 mm. Enhanced CT scan was performed with a rapid intravenous injection of 100 mL iothalamate meglumine （Ultravist） （370 mgI/mL） or Omnipaque （350 mgI/mL） and 30 mL 0.9% NaCl chaser bolus at a flow rate of 3.5 mL/s. Calcium scoring with plain scan images and two and three dimensional reconstruction with enhanced scan images were made in all cases, among which 30 cases underwent conventional coronary angiography. Demonstration of coronary arteries and their stenosis were evaluated and the factors that might influence the image quality were analyzed. Results Coronary calcium scores were calculated and coronary artery was demonstrated in our study. In the evaluationof image quality with volume rendering technique （VRT） images, 78.3% of the images were of the first class, 12.2% the sec-ond class, and 9.6% the third class. Multi-planar reconstruction （MPR） and maximal intensity projection （MIP） were better than VRT in the demonstration of small branches. The image quality was related to the heart rate, with or without arrhythmia, and breath-hold ability of patients. Comparative study of the stenosis of coronary arteries in 30 cases showed that the sensi-tivity and specificity of 16-slice coronary CT angiography （CTA） to diagnose significant stenosis were 95.8% and 94.8% resp-ectively. Conclusion As a non-invasive and quick method, 16-slice coronary CTA is sensitive and specific to diagnose the stenosis of coronary arteries and can be used as a screening method in the diagnosis of CHD.Keywords：angiography;tomography, X-ray computed;coronary artery4|12|8Updated：2024-04-10
- Abstract：To explore the protective effect of tetramethylpyrazine （TMP） on the learning and memory function in D-galactose （D-gal）-lesioned mice. Methods C57BL/6 mice were injected （s.c.） 2% D-gal for 40 days （100 mg·kg-1·d-1）. Normal saline, TMP, and Huper-zine A were respectively given by intragastric administration in different groups from the third week. Learning and memory ability was tested with Morris water maze for 5 days at the sixth week. After completion of behavioral test, the mice were sacrificed by decapitation. The brain was rapidly removed, and the cortex and hippocampus were separated. The superoxide dismutase （SOD） activity and malondialdehyde （MDA） content in the cortex were determined. At the same time, the activity of choline acetyltransferase （ChAT） and acetylcholinesterase （AChE）, the binding sites （Bmax） and the affinity （KD） of M-cholinergic receptor in the cortex, and Bmax and KD of N-methyl-D-aspartate （NMDA） receptor in the hippocampus were determined. Results In this model group, （1） The deficit of learning and memory ability, （2） elevated MDA content and lowered SOD activity, （3） decreased AChE activity and M-cholinergic receptor binding sites in the cortex, and （4） lowered NMDA receptor binding sites were observed in the hippocampus, as compared with the normal control. TMP could markedly （1） attenuate cognitive dysfunction, （2） lower MDA content and elevate SOD activity, （3） increase the activity of ChAT and AChE, and M-cholinergic receptor binding sites in the cortex in the mice treated with D-gal. NMDA receptor binding sites were also increased in the hippocampus in the treated mice. Conclusion TMP can significantly strengthen antioxidative function, improve central cholinergic system function, protect NMDA receptor activity, and thus enhance the learning and memory ability in D-gal-lesioned mice.Keywords：tetramethylpyrazine;D-galactose;Alzheimer’s disease;cholinergic system;N-methyl-D-aspartate receptor4|35|10Updated：2024-04-10
- Abstract：To measure Derp1 and Blot5 allergen levels in asthmatics’ homes in Hongkong. Methods Seventy houses were enrolled for a mite indoor environment study. Dust samples were obtained from two sites of each patients’ house: bed and floor. Derp1 and Blot5 levels were quantified by a two-site monoclonal antibody-based ELISA technique. Results The levels of Derp1 allergens found in bed （geometric mean （GM） 3.43 μg/g of dust; 95%CI, 1.89-4.96 μg/g） and on the floor （GM 1.12 μg/g of dust; 95%CI, 0.71-1.53 μg/g） indicated significant differences （P = 0.005）. However, the levels of Blot5 allergens found in bed （GM 19.00 μg/g of dust; 95%CI, 0.89-38.90 μg/g） and on the floor （GM 6.14 μg/g of dust; 95%CI, 0.40-11.90 μg/g） showed no statistically significant difference. In addition, in regards to the exposure index for Derp1 and Blot5 allergens found in bed and on the floor, 17.6% in bed and 8.6% on the floor had levels of Blot5 ≥ 10 μg/g of dust, higher than those obtained for Derp1 （7.2% and 0% in bed and on the floor respectively, P < 0.05）; higher percentages in bed and on the floor （25.0% and 35.7%） were observed for levels of Blot5 = 0 μg/g of dust as compared with Derp1 in bed and on the floor （4.3% and 14.5% respectively, P < 0.05）. Conclusions Derp1 and Blot5 are the major allergens found in this regional study, Blot5 is a more potent allergen in Hongkong, probably reflecting the high level of exposure to Blomia tropicalis （Bt）. Bt and Dermatophagoides pteronyssinus（Dp） allergens should be included for precise diagnosis and effective immuno-therapeutic treatment of mite allergy in Hong-kong.Keywords：house dust mite;Derp1;Blot5;asthma4|24|10Updated：2024-04-10
- Abstract：To investigate the effects of early nutritional intervention on the serum insulin-like growth factor-1 （IGF1）,insulin-like growth factor binding protein 3 （IGFBP3）, intestinal development, and catch-up growth of intrauterine growth retardation （IUGR） rats by giving the IUGR new born rats different protein level diet. Methods IUGR rat model was built by starvation of pregnant female rats. Twenty-four IUGR pups and 8 normal pups were divided randomly into 4 groups: normal control group （C group）; IUGR control group（S group）, IUGR low-protein diet group （SL group）, and IUGR high-protein diet group （SH group）. Detected the serum IGF1, IGFBP3, body weight, body length, intestinal weight length, intestinal villi height （VH）, crypt depth （CD）, villi absorbing area （VSA）, mucous thickness （MT）, and disaccharidase at the 4th week. Results （1） The SH group showed the fastest catch-up growth, serum IGF1, IGFBP3, VH, and VSA were significantly higher than those of normal control group and IUGR control group. The intestinal weight and length, and the activities of lactase and saccharase of the SH group also reached the normal control group level. （2） The SL group kept on small size, the serum IGF1, IGFBP3, and most of intestinal histological indexes were all significantly lower than other groups. （3） IGF-1, IGFBP3 were positively correlated to intestinal VH, VSA, saccharase, body weight and length. Conclusions The serum IGF1 was a sensitive index to the catch-up growth. The early nutritional intervention of high-protein diet after birth is helpful for the catch-up growth of IUGR through promoting the intestinal development and the ab-sorption of nutritionKeywords：intrauterine growth retardation;nutritional intervention;insulin-like growth factor-14|35|4Updated：2024-04-10
- Abstract：To investigate the roles of maspin and kai1 expression in tumorigenesis and progression of gastric cancer. Methods Maspin and kai1 expressions were detected in normal gastric mucosa （n = 182）, gastric dysplasia （n = 69）, and gastric cancer （n = 113） by immunohisto-chemistry. Their expressions were compared with clinicopathological parameters of tumors. Relationship between maspin and kai1 expression was also concerned in gastric cancer. Results The positive rates of maspin expression were 79.8% （145/182）, 75.4% （52/69）, and 50.4% （57/113） in normal gastric mucosa, gastric dysplasia, and gastric cancer, while those of kai1 expression were 81.9% （149/182）, 65.2% （49/69）, and 58.4% （66/113） in corresponding tissues respectively. Gastric cancer less frequently expressed maspin than the normal gastric mucosa and gastric dysplasia （P < 0.05）, while dysplasia and cancer showed less frequent expression of kai1 than normal mucosa （P < 0.05）. Maspin expression showed negative association with invasive depth, metastasis, Lauren’s and histological classifications （P < 0.05）, but not with tumor size, Borrmann’s classification, growth pattern or TNM staging （P > 0.05）. Kai1 expression was negatively correlated with invasive depth, metastasis, growth pattern, Lauren’s and histo-logical classifications （P < 0.05）, but not with tumor size, Borrmann’s classification or TNM staging （P > 0.05）. Maspin and kai1 were collaboratively expressed in gastric cancer （P < 0.05）. Conclusions Down-regulated expressions of maspin and kai1 play an important role in gastric carcinogenesis. Abnormal expression of maspin and kai1 might have inhibitory effects on invasion and metastasis of gastric cancer and act as an effe-ctive and objective marker to indicate the pathobiological behaviors of gastric cancer.Keywords：maspin;kai1;carcinogenesis;progression;stomach neoplasms4|59|32Updated：2024-04-10
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- Abstract：To investigate effect of the soluble epidermal growth factor receptor （sEGFR/sErbB1） level in the periph-eral blood in development, invasiveness, apoplexy of each type of pituitary tumor. Methods The sEGFR level was determined in peripheral serum from 190 patients with pituitary diseases by enzyme linked immunosobent assay. The sEGFR levels were measured in 10 pituitary Rathke’s pouch, 18 pituitary hyperplasia, 161 pituitary adenomas including 30 microadenomas, 83 large adenomas, 48 giant adenomas, 1 pituitary carcinoma, and 28 hea-lthy controls. Results In the patients with pituitary hyperplasia, microadenoma, large adenoma, giant adenoma, and pituitary carci-noma, the sEGFR level was 188.92 ± 32.62, 209.83 ± 19.01, 333.20 ± 69.33, 405.85 ± 37.38, and 617.45 fmol/mL indepen-dently. They were all significantly higher than patients with pituitary Rathke’s pouch （156.78 ± 18.24 fmol/mL, P < 0.001） and healthy control group （159.11 ± 40.50 fmol/mL, P < 0.05）. The sEGFR level in pituitary carcinoma was higher than pi-tuitary adenoma. In patients with pituitary adenoma, the sEGFR level was positive correlated to the size of pituitary adeno-mas （r = 0.998）, the significant difference was observed for the sEGFR level in each group of the patients with pituitary adenomas （P < 0.001）. Furthermore, in patients with pituitary ACTH-secreting microadenomas, the serum sEGFR levels in invasiveness （295.00 ± 77.80 fmol/mL） was higher than that in non-invasiveness （210.60 ± 16.4 fmol/mL, P < 0.05）. In pati-ents with pituitary ACTH-secreting, PRL-secreting, GH-secreting, and non-functioning large adenomas, the serum sEGFR levels in invasiveness （407.86 ± 28.50, 399.25 ± 30.10, 386.00 ± 13.08, and 369.25 ± 36.70 fmol/mL） was higher than that in non-invasiveness （335.25 ± 63.49, 300.64 ± 47.57, 297.00 ± 61.93, and 269.30 ± 25.68 fmol/mL） respectively （P < 0.05）. In patients with invasive pituitary PRL-secreting, GH-secreting, and non-functioning giant adenomas, the serum sEGFR levels not significantly different in between invasiveness （417.50 ± 35.94, 409.50 ± 69.14, and 417.50 ± 44.13 fmol/mL） and non-invasiveness （386.00 ± 49.64, 417.50 ± 44.03, and 409.51 ± 35.17 fmol/mL） （P > 0.05）. In patients with pituitary large adeno-mas, the sEGFR levels in pituitary apoplexy （377.48 ± 39.18 fmol/mL） was higher than that in non-pituitary apoplexy （343.18 ± 68.17 fmol/mL, P > 0.05）. Conclusions The increased level of peripheral serum sEGFR is concomitant with development, proliferous size of the adenomas in patients with pituitary adenomas. In addition, the elevated levels of serum sEGFR occur in pituitary apoplexy as clinical active tumors, and the non-invasive ACTH secreting adenomas. The sEGFR levels could be differen-tiated helpfully between pituitary adenomas and non-pituitary adenomas. These data suggest that serum sEGFR could be as a referable marker of the size and activation of proliferation in pituitary adenoma.Keywords：pituitary adenoma;pituitary carcinoma;soluble epidermal growth factor receptor4|9|4Updated：2024-04-10
- Abstract：To determine the clinical application of pulsed Doppler tissue imaging in assessing the left ventricular diasto-lic function and in discriminating between normal subjects and patients with hypertrophic cardiomyopathy with various stages of diastolic dysfunction. Methods We measured the peak diastolic velocities of mitral annulus in 81 patients with hypertrophic cardiomyopathy with various stages of diastolic dysfunction and 50 normal volunteers by Doppler tissue imaging using the apical window at 2-ch-amber and long apical views, respectively. The myocardial velocities were determined with use of variance F statistical analysis. Results Early diastolic myocardial velocities of mitral annulus were higher in normal subjects than in patients with hy-pertrophic cardiomyopathy with either delayed relaxation, pseudonormal filling, or restrictive filling. However, peak myocar-dial velocities of mitral annulus during atrial contraction were similar in normal subjects and patients with hypertrophic cardiomyopathy. Conclusion Doppler tissue imaging can directly reflect upon left diastolic ventricular function. Early phase of diastole was the best discriminator between control subjects and patients with hypertrophic cardiomyopathy.Keywords：Doppler tissue imaging;left ventricular diastolic function;hypertrophic cardiomyopathy4|3|2Updated：2024-04-10
- Abstract：To establish a cell line for stable expression of human beta-defensin 3 （hBD3）. Methods Full length cDNA of hBD3 was isolated from previously constructed pGEM-hBD3 and then inserted into pcDNA3. The recombinant vector identified carrying hBD3 with right direction was introduced into COS-7 cells by Lipofe-ctamine. Cell clones survived in G418-rich medium and with stable expression of hBD3 in both mRNA and protein levels were identified by RT-PCR and Western blot respectively. Genomic integration of the hBD3 gene with the COS-7 cells was confirmed by Southern dot blot and primary analysis. The antimicrobial activity of the secreted hBD3 was also evaluated. Results COS-7 cells transfected with pcDNA3-hBD3 expressed hBD3 stably in mRNA and protein level. Southern dot blot analysis showed successful integration of the hBD3 gene into the genome of COS-7 cell and the hBD-3 protein secreted into the culture medium showed antimicrobial activity. Conclusion We successfully established a hBD3-expressing cell line.Keywords：human beta-defensin 3;eukaryotic expression;gene transfection4|22|1Updated：2024-04-10
- Abstract：To detect serum HER-2 oncoprotein levels in patients with operable and metastatic breast cancers, and to study the correlations between serum HER-2 level and lymph node status as well as other clinical parameters. Methods A total of 120 women were studied consisting of 10 healthy volunteers, 31 benign breast disease, 53 operable breast cancer, and 26 metastatic breast cancer patients. The levels of serum HER-2 were measured using an enzyme-liked im-munosorbent assay （ELISA）. Results The mean serum HER-2 levels were 9.6 ± 1.5 ng/mL in healthy volunteers, 11.9 ± 1.6 ng/mL in benign breast disease, 13.2 ± 4.2 ng/mL in operable breast cancer, and 30.5 ± 30.8 ng/mL in metastatic breast cancer patients. The former is much lower than the latter three （P = 0.02, 0.001, 0.03, respectively）. If using 15 ng/mL as a normal baseline, elevated serum HER-2 levels were observed in none of the healthy volunteers as well as patients with benign disease, but in 18.9% （10/53） operable breast cancer patients and 61.5% （16/26） metastatic patients. In patients with operable breast cancer, there was a positive correlation between serum concentrations of HER-2 and the size of primary tumor （P < 0.05）, whereas there was no correlation between serum concentration and axillary lymph node or estrogen receptor status. In patients with metastatic dise-ase, there was no correlation with site of metastases （P > 0.05）. Conclusion Serum HER-2 level was strongly correlated with tumor loads and clinical stages, thus acting as a promising predictor of cancer recurrence in breast cancer patients.Keywords：breast cancer;serum HER-2;ELISA4|18|11Updated：2024-04-10
- Abstract：To define the criteria of posterior selective thoracic fusion in patients with adolescent idiopathic scoliosis. Methods By reviewing the medical records and roentgenograms of 17 patients with adolescent idiopathic scoliosis who un-derwent posterior selective thoracic fusion, the curve type, Cobb angle, apical vertebral rotation and translation, trunk shift, and thoracolumbar kyphosis were measured and analyzed. Results There were 17 King type Ⅱ patients （PUMC type: Ⅱb1 13, Ⅱc3 4）. The coronal Cobb angle of thoracic curve be-fore and after operation were 56.9°and 21.6° respectively, the mean correction rate was 60.1%. The coronal Cobb angle of lumbar curve before and after operation were 34.8° and 12.1° respectively, and the mean spontaneous correction rate was 64.8%. At final follow-up, the coronal Cobb angle of thoracic and lumbar curve were 23.5° and 15.2° respectively, there were no significant changes in the coronal Cobb angle, apical vertebral translation and rotation compared with that after operation. One patient had 12° of thoracolumbar kyphosis after operation, no progression was noted at final follow-up. There was no trunk decompensation or deterioration of the lumbar curve. In this group, 3.9 levels were saved compared with fusing both the th-oracic and lumbar curves. Conclusion Posterior selective thoracic fusion can be safely and effectively performed in King type Ⅱ patients with a mo-derate and flexible lumbar curve, which can save more mobile segments and at the same time can maintain a good coronal and sagittal balance.Keywords：adolescent idiopathic scoliosis;selective thoracic fusion4|16|3Updated：2024-04-10
- Abstract：To express and purify the protein coded by the TRAF-type zinc finger domain of myasthenia gravis （MG）-re-lated gene P9 （ P9-ZFD ） and to prepare P9-ZFD antiserum for detecting expression and subcellular distribution of P9-ZFD protein in the skeletal muscles of patient with MG. Methods The cDNA encoding P9-ZFD was amplified by RT-PCR. The cloned P9-ZFD cDNA was ligated into pET-24a, and the P9-ZFD recombinant protein was induced via E.coli. BL21 （DE3） and purified by histidine affinity chromato-graphy. P9-ZFD antiserum was prepared and its titer and specificity were determined by ELISA and Western blot. Expres-sion and subcellular distribution of P9-ZFD protein in the skeletal muscles of MG and control were studied. Results The molecular weight of purified P9-ZFD protein was about 30 kD. Its purity was more than 95%. Antiserum specific for P9-ZFD was excellent. P9-ZFD protein is fully confined to the cytoplasm membrane of skeletal muscle cell of MG, obvious immunostaining was absent in the A, I, and Z bands of cytoplasm and no immunoreactivity was observed in the skeletal muscle cell of control. Conclusion P9-ZFD protein is expressed as a cytoplasm membrane-bound protein and has obvious distribution difference in the skeletal muscle cells of patient with MG and normal control.Keywords：myasthenia gravis;skeletal muscle;P9-ZFD gene fragment4|12|0Updated：2024-04-10
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EFFECTS OF CHINA-MADE RECOMBINANT HUMAN GROWTH HORMONE ON THE TREATMENT OF GROWTH HORMONE DEFICIENCY
Abstract：To evaluate the therapeutic effect of China-made recombinant human growth hormone （r-hGH） in children with growth hormone deficiency （GHD） and to investigate the utilities of various biochemical parameters in GHD diagnosis and treatment. Methods Our study comprises of 30 normal children and 71 GHD children treated with China-made r-hGH substitution therapy 0.1 IU·kg-1·d-1 for 6 months. Serum insulin-like growth factor-1 （IGF-1）, insulin-like growth factor binding protein 3 （IGFBP-3）, bone turnover markers （Ost, ICTP）, and anti-growth hormone antibody （GHAb） were detected before and after r-hGH treatment. Results After the first 3 and 6 months of treatment, growth velocities of GHD children were significantly increased （13.1 ± 3.7 and 12.6 ± 3.6 cm/year） compared with pretreatment values （2.9 ± 0.8 cm/year, P < 0.01）. GHD Children had obviously reduced serum levels of IGF-1, IGFBP-3, and bone turnover markers （Ost, ICTP） compared with normal controls （P < 0.01）, and these biochemical parameters improved significantly after treatment （P < 0.01）. Growth hormone antibodies were positive in 17 of 45 cases after treatment by binding capacity detection. The binding percentage of growth hormone an-tibody which was increased more than 30% after the treatment showed a negative correlation with growth velocity （P < 0.01）. Conclusions （1） The growth stimulating effect and safety were confirmed in using China-made r-hGH in the treatment of GHD children for 6 months. （2） The measurements of serum IGF-1 and IGFBP-3 may serve as useful parameters in the diagnosis of GHD. （3） Serum Ost and ICTP are useful laboratory criteria for evaluating the effect of r-hGH therapy in the early stage. （4） It is necessary to monitor serum levels of GHAb during r-hGH therapy.Keywords：somatotropins;recombinant;growth hormone deficiency;child4|8|0Updated：2024-04-10 - Abstract：To test the accuracy of real-time three-dimensional echocardiography （RT3DE） imaging system for evaluating left ventricular mass （LVM） in phantom and excised canine heart. Methods Ten left ventricular （LV） wall phantoms made of two rubber-bursas, ten excised canine hearts underwent RT3DE and two-dimensional echocardiography （2DE）. In RT3DE "full volume" imaging, the myocardial volume was mea-sured using 2,4,8, and 16-plane method with the analysis software of RT3DE. Mass was then calculated by multiplying the resulting myocardial volume by specific density of myocardial tissue. In 2DE the masses were measured by area-length meth-od. The true LV wall phantom mass was measured by water displacement and the canine LVM was weighed by anatomy, which served as a reference standard. We compared RT3DE or 2DE with true mass. Results In LV wall phantoms, RT3DE correlated with true masses strongly （r = 0.813-0.994） and weakly correlated between 2DE and true masses （r = 0.628）. In excised canine hearts, there is an excellent correlation between RT3DE and true masses （r = 0.764-0.991）, while 2DE value showed a lesser correlation （r = 0.514）. There are no difference between RT-3DE and true masses （P > 0.05） but different between 2DE and true masses （P < 0.05）. In different planes, there was no difference between 8-plane and 16-plane （P > 0.05） but different between 8-plane and 2, 4-plane （P < 0.05）. Conclusion RT3DE can accurately quantify LVM and provide a new tool to evaluate LV function. For LVM by RT3DE, 8-plane measurement method is the best choice for accuracy and convenience.Keywords：real-time three-dimensional;echocardiography;left ventricular mass4|8|7Updated：2024-04-10
- Abstract：To construct a DNA vaccine as a prophylactic model to prevent condyloma acuminatum and detect its immu-nogenicity in mice. Methods The major capsid protein （L1） gene of human papillomavirus （HPV） 6b was inserted into an eukaryotic ex-pression plasmid （pcDNA3.1）. The recombinant plasmid was transfected into COS-7 cells. Western blot were performed to detect whether L1 protein can be expressed in eukaryotic cells. Eighteen female BALB/c mice were tested for immunoge-nicity study. Results The recombinant plasmid （pcDNA3.1-HPV6bL1） was verified as HPV6b L1 gene by sequencing. Western blot showed specific strip. Anti-L1 protein antibodies could be detected in the mice’s sera inoculated with pcDNA3.1-HPV6bL1. Similarly, IL-4, IL-2, and IFN-γ were increased in the same mice. Conclusion HPV6b L1 recombinant plasmid was constructed successfully which had immunogenicity for BALB/c mice. It provided experimental evidence for the research of DNA vaccine of condyloma acuminata..-Keywords：condyloma acuminata;human papillomavirus;DNA vaccine4|19|1Updated：2024-04-10
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