Latest Issue
Volume 27 ， Issue 1 ， 2012
- Abstract：ObjectiveTo screen the asymmetric dimethyl arginines (ADMA)-containing proteins which could combine with protein arginine methyltransferase 1 (PRMT1). Methods Western blot was adopted to identify the expression of PRMT1 and the proteins with ADMA in glioma cell lines and normal brain tissues, and then to detect the changes of ADMA level after knock-down of PRMT1 with RNAi transfection in U87MG cells. Co-Immunoprecipitation (Co-IP), western blot, and sliver staining were employed to screen the candidate binding proteins of PRMT1. Then liquid chromatography-tandem mass spectrometry (LC-MS/MS) was used to identify the binding proteins of PRMT1. Results The expression of PRMT1 and some levels of ADMA were higher in glioma cell lines than in normal brain tissues. After knocking down PRMT1, some ADMA levels were found declined. After screening the binding proteins of PRMT1 with Co-IP and LC-MS/MS, 26 candidate binding proteins were identified. Among them, 6 candidate proteins had higher ions scores (>38) and bioinformation analysis predicted that SEC23-IP, ANKHD1-EIF4EBP3 protein, and 1-phosphatidylinositol-3-phosphate 5-kinase isoform 2 had possible methylated aginine sites. Conclusions The high expression of PRMT 1 in glioma may induce the change of ADMA levels. Altogether 26 candidate proteins were identified, which contain ADMA and specifically bind with PRMT1.Keywords：protein arginine methyltransferase 1;asymmetric dimethyl arginines;liquid chromatography-tandem mass spectrometry4|3577|0Updated：2024-04-10
- Abstract：ObjectiveTo determine the spatio-temporal expression of p70S6k activation in hippocampus in mesial temporal lobe epilepsy. Methods Temporal lobe epilepsy model was established by stereotaxically unilateral and intrahippocampal injection of kainite acid (KA) in adult male C57BL/6 mice. Latent and chronic epileptogenesis were represented by mice 5 days after KA injection ( n=5) and mice 5 weeks after KA injection ( n=8), respectively. Control mice ( n=5) were injected with saline. Immunohistochemical assays were performed on brain sections of the mice. Results Hippocampus both ipsilateral and contralateral to the KA injection displayed significantly up-regulated pS6 immunoreactivity in dispersed granule cells in 5-day and 5-week model mice. Conclusion The activation of p70S6k is mainly located in the dentate gyrus in KA-induced mouse model of temporal lobe epilepsy, indicating that the activation may be related with the disperse degree and hypertrophy of granule cells.Keywords：mesial temporal lobe epilepsy;p70S6k;kainate acid;immunoreactivity4|2633|0Updated：2024-04-10
- Abstract：ObjectiveTo examine the inhibitory effects of recombinant purified arresten on tumor formation. Methods Purified arresten protein was incubated with human umbilical vein endothelial cells (HUVECs) and HeLa cells in vitro. The effect on proliferation of HUVECs and HeLa cells was examined using 3-(4,5)-dimethylthiahiazo (-z-y1)-3,5-di-phenytetrazoliumromide assay, and apoptosis of these cells monitored by flow cytometry. The effect on migration of HUVECs and HeLa cells was examined by Boyden chamber. Twenty colon carcinoma-bearing C67BL/6 mice were used to investigate the antitumor effects of arresten protein. The mice were randomly divided into arresten treatment group ( n=10) and control group ( n=10). The microvessel densities of the tumors were measured by immunohistochemical staining with anti-CD31 monoclonal antibody. Results Arresten inhibited the proliferation and migration of HUVECs in a dose-dependent manner while promoting apoptosis. However, arresten had no significant effects on the proliferation and apoptosis of HeLa cells. The migration of HeLa cells was modestly inhibited by arresten. The arresten treatment group of mice showed no weight loss or unusual behavior during the course of treatment, and the tumor growth was significantly decreased; in contrast, the control group of mice exhibited rapidly growing tumors and cachexia. A dramatically decreased microvessel density in tumor tissues was found in arresten-treated mice compared with that in the control mice. Conclusion Arresten can inhibit tumor growth through inhibition of tumor angiogenesis.Keywords：arresten;prokaryotic expression;purification;tumor4|2551|0Updated：2024-04-10
- Abstract：ObjectiveTo elucidate GPR40/FFA1 and its downstream signaling pathways in regulating insulin secretion. Methods GPR40/FFA1 expression was detected by immunofluorescence imaging. We employed linoleic acid (LA), a free fatty acid that has a high affinity to the rat GPR40, and examined its effect on cytosolic free calcium concentration ([Ca2+]i) in primary rat β-cells by Fluo-3 intensity under confocal microscopy recording. Downregulation of GPR40/FFA1 expression by antisense oligonucleotides was performed in pancreatic β-cells, and insulin secretion was assessed by enzyme-linked immunosorbent assay. Results LA acutely stimulated insulin secretion from primary cultured rat pancreatic islets. LA induced significant increase of [Ca2+]i in the presence of 5.6 mmol/L and 11.1 mmol/L glucose, which was reflected by increased Fluo-3 intensity under confocal microscopy recording. LA-stimulated increase in [Ca2+]i and insulin secretion were blocked by inhibition of GPR40/FFA1 expression in β-cells after GPR40/FFA1-specific antisense treatment. In addition, the inhibition of phospholipase C (PLC) activity by U73122, PLC inhibitor, also markedly inhibited the LA-induced [Ca2+]i increase. Conclusion LA activates GPR40/FFA1 and PLC to stimulate Ca2+ release, resulting in an increase in [Ca2+]i and insulin secretion in rat islet β-cells.Keywords：GPR40 /FFA1;phospholipase C;antisense oligonucleotides;intracellular calcium;linoleic acid4|2467|0Updated：2024-04-10
- Abstract：ObjectiveTo study the clinicopathological features of patients with urothelial carcinoma of the urinary bladder (UCB), and analyze the association of clinicopathological characteristics with tumor recurrence and progression. Methods Altogether 658 UCB cases in Fudan University Shanghai Cancer Center were collected from January 2006 to December 2010. The histopathologic materials and the clinical records were reviewed. Univariate and multivariate analyses were preformed to detect the association. Results The mean age of the patients was 61.97 ±12.97 years (range, 20-90 years). Male to female ratio was about 5:1. A total of 517 cases (78.6%) were superficial at the time of diagnosis (stage Ta/T1). The mean follow-up period was 22.36 ±24.92 months. Twenty-five patients lacking follow-up information were excluded in calculating recurrence and progression rates, the recurrence rate was about 37.0% (234/633), and progression rate about 6.2% (39/633). Three variables (grade, tumor growth pattern, and pathological stage) were found to be significant risk factors for tumor progression in univariate and multivariate analyses ( P<0.05). Conclusions Most of the newly diagnosed UCB cases may be superficial diseases. Grade, tumor growth pattern, and pathological stage are associated with tumor progression of UCB.Keywords：urinary bladder;urothelial carcinoma;tumor growth pattern;recurrence;progression6|2895|0Updated：2024-04-10
- Abstract：ObjectiveTo provide novel spectral domain optical coherence tomography (SD OCT) findings of Vogt-Koyanagi-Harada (VKH) disease as well as new insights into the pathogenesis of this disease. Methods Detailed SD OCT and fluorescein angiography (FA) findings of 18 consecutive VKH patients (11 women and 7 men) from December 2007 to April 2009 who were in acute uveitic stage at presentation were reviewed. All the patients had been followed up for at least 6 months with reevaluation(s) of SD OCT performed in 10 patients. Results Intraretinal cysts were found to be located in various layers of the outer retina. In addition to the photoreceptor layer, t hey could also be found between the outer plexiform layer and the outer nuclear layer, or spanning the external limiting membrane. On FA, intraretinal cysts could be hypofluorescent, normofluorescent, or hyperfluorescent. Some intraretinal cysts had a characteristic FA pattern, in which a small round hypofluorescent area was surrounded by a ring of hyperfluorescence (donut-shaped dye pooling). Subretinal fibrinoid deposit appeared in acute uveitic stage in two severe VKH patients and seemed to develop from subretinal exudates and evolved into typical subretinal fibrosis. Gradual transfiguration/migration and progressive proliferation/pigmentation of the subretinal fibrinoid deposit/subretinal fibrosis was observed in one patient. Conclusions Intraretinal cysts could form in various layers of the outer retina and may result from extension of choroidal inflammation. Subretinal fibrosis may develop from subretinal exudates in VKH patients and may cause substantial visual impairment.Keywords：Vogt-Koyanagi-Harada disease;spectral domain optical coherence tomography;fluorescein angiography;subretinal fibrosis;intraretinal cysts6|2827|0Updated：2024-04-10
- Abstract：ObjectiveTo summarize the management of anastomotic leak following surgery for esophageal carcinoma. Methods The medical records of the patients developing digestive tract leak after surgery for esophageal carcinoma in our hospital from January 2003 to March 2011 were retrospectively analyzed. Results A total of 36 patients were included, in whom 13 developed cervical anastomotic leak, 18 had intra-thoracic anastomotic leak, and 5 had intra-thoracic gastric necrosis. Of these patients, 7 were treated with resurgery, 6 with esophageal stent implantation, and 23 with conservative treatment. Treatment lasted for 5 to 181 days, averagely 47.0?31.9 days. After management, 9 patients died (25.0%). Among seven patients with resurgery, four had deceased, two were cured, and one developed leak again and was switched to conservative treatment until discharged. All the 6 patients treated with stent implantation were cured. Of the 24 patients receiving conservative treatment (including one switched from resurgery), 18 (75.0%) were cured and 1 was not cured but survived. Conclusions Anastomotic leak following surgery for esophageal carcinoma should be treated individually based on the onset time, location, size, and extent of the leakage. Conservative treatment is still a safe and effective method. The efficacy of stent implantation needs further investigation to confirm.Keywords：esophageal surgery;anastomotic leak;resurgery;stent4|2871|0Updated：2024-04-10
- Abstract：ObjectiveTo assess whether Community Periodontal Index (CPI) teeth and random half-mouth methods are representative of full- mouth method in gingival bleeding examination. Methods Data from 1000 untreated adults (age > 18) collected in Beijing from January 2000 to January 2001 were utilized in the analysis. Half of the subjects were examined by Gingival Bleeding Index (GBI) and the other half by Sulcus Bleeding Index (SBI). The data were sorted out and calculated according to CPI teeth and random half-mouth teeth methods. The results of the 2 methods were separately compared with those of full-mouth examination. Results There was a significant difference between CPI teeth method and full-mouth examination ( P0.05), nor in diagonal half-mouth results in GBI ( P>0.05) Conclusions The results of CPI teeth method on gingival bleeding could not reflect the full-mouth situation. Ipsilateral and diagonal half-mouth results can serve as a substitute for full-mouth results in SBI, while diagonal half-mouth results in GBI.Keywords：Community Periodontal Index;half-mouth examination;full-mouth examination;Gingival Bleeding Index;Sulcus Bleeding Index;epidemiology4|3065|0Updated：2024-04-10
- Abstract：ObjectiveTo discuss the interaction between pregnancy and ankylosing spondylitis, and the management of pregnancy with ankylosing spondylitis. Methods Twelve cases of pregnancy with ankylosing spondylitis in Peking Union Medical College Hospital from September 2004 to July 2011 were analyzed retrospectively, focusing on the arteritis condition, pregnancy complications, and outcomes. Results All the 12 patients had full-term pregnancy. Five cases gave birth naturally, and 7 cases received cesarean section for maternity factors. No adverse pregnancy outcomes were encountered. Waist pain appeared in 2 cases in the second trimester, for both of which medication failed. One of the 2 cases had natural childbirth, while the other maintained pregnancy smoothly to cesarean section. Conclusions Pregnancy monitoring can help obtain favorable pregnancy outcomes. Attention should be paid to postpartum change of the illness.Keywords：ankylosing spondylitis;pregnancy;pregnancy outcome4|2970|0Updated：2024-04-10
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Primary Sjören's Syndrome Accompanied by Intestinal Obstruction: a Case Report and Literature Review
Abstract：SJóREN'S syndrome (SS) is a chronic inflammatory autoimmune disease characterized by the infiltration of lymphocytes and plasma cells in exocrine glands, especially salivary and lacrimal gland interstitium. The clinical manifestations of SS are complex. When the digestive system is involved, the patient always presents with liver damage, atrophic gastritis, and chronic diarrhea. Intestinal obstruction is rare and the specific pathogenesis remains unclear.1 Currently, there is no clear treatment guidelines for SS with intestinal obstruction. The general measures include gastrointestinal decompression, spasmolysis, and other symptomatic treatments and the use of glucocorticoid to control the progressing of disease. Here we describe a case of primary SS with intestinal obstruction who was treated with conservative treatment.Keywords：Sjóren's syndrome;intestinal obstruction6|2494|0Updated：2024-04-10 - Abstract：ELECTROCORTICOGRAPHY (ECoG), the intra- operative recording of cortical potentials, has played an important role in the surgical management of patients with medically intractable epilepsy. This technique is useful in epilepsy surgery to delineate margins of epileptogenic zones, guide resection, and evaluate completeness of resection.1 About one third of the patients with intractable epilepsy have obvious causes, such as tumor, sclerosis, or vascular malformation. However, the presence of tumor in the eloquent cortex like speech area makes it challenging to completely resect the tumor. ECoG-based surgery in conjunction with direct cortical stimulation in such cases helps surgical resection of tumor with no functional tissue affected, producing good surgical outcome. This report describes a case treated with ECoG-based surgery in combination with direct cortical stimulation.Keywords：electrocorticography;intractable epilepsy;direct cortical stimulation;speech area4|4834|0Updated：2024-04-10
- Abstract：HEPATIC paragonimiasis is a rare disease with non-specific manifestation, which is often misdiagnosed in clinical practice. Long-time drug therapy treating this disease would be accompanied by the possible development of side effects. Given the fact that this disease has seldom been reported in the literature, we provide in this article a detailed report of one case with hepatic paragonimiases treated by radiofrequency ablation, which brought favorable outcome, aiming to shed some light on the understanding of this rare condition.Keywords：radiofrequency ablation;paragonimiasis;liver6|2626|0Updated：2024-04-10
- Abstract：MORTALITY rate of acute myocardial infarction (AMI) patients could be as high as 80%, if present with or develop fatal arrhythmia and cardiac arrest. Some patients, despite the treatment with inotropes and intra-aortic balloon pump, are not hemodynamically stable enough to undergo emergent revascularization. The use of extracorporeal membrane oxygenation (ECMO) can help stabilize circulation and respiration, which could be a potential solution for patients under such critical circumstances. In this paper, we describe the application of ECMO for the resuscitations of 4 cardiac arrested AMI cases.4|3143|0Updated：2024-04-10
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