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Volume 28 ， Issue 2 ， 2013
- Abstract：SIRT1 and FasL protein levels were detected by Western blotting in either mouse arteries extract or the whole rat aortic vascular smooth muscle cell (VSMC) lysate. Smooth muscle cell (SMC)-specific human SIRT1 transgenic (Tg) C57BL/6 mice and their littermate wild-type (WT) controls underwent complete carotid artery ligation (ligation groups) or the ligation-excluded operation (sham groups). The carotid arteries were collected 1 day after operation. Reverse transcription-polymerase chain reaction was performed to detect the mRNA levels of SIRT1 and FasL. Luciferase reporter assays were performed to detect the effect of WT-SIRT1, a dominant-negative form of SIRT1 (SIRT1H363Y), and GATA-6 on the promoter activity of FasL. Flow cytometry assay was applied to measure the hypodiploid DNA content of VSMC so as to monitor cellular apoptosis.Keywords：sirtuin1;Fas ligand;GATA-6;vascular smooth muscle cell6|1072|0Updated：2024-04-10
- Abstract：Literatures published up to April 2012 were retrieved from PubMed, Embase and Cochrane library, Chinese National Knowledge Infrastructure (CNKI), Chinese Scientific and Technological Periodical Database (VIP), Wangfang, and Traditional Chinese Medicine online. Two researchers collected data independently. The assessment of methodological quality was based on Cochrane handbook and the materials were analyzed with software RevMan 5.1.2. The outcome measure index was relative risk or difference of mean value (95% confidence interval). The following outcomes were evaluated (1) general clinical improvement rate; (2) maximum vertical pocket depth; (3) amniotic fluid index (AFI) value; (4) rate of fetal ductus arteriosus constriction; (5) incidence of adverse events.Keywords：Chinese herbal medicine;polyhydramnios;meta-analysis;systematic review4|2527|0Updated：2024-04-10
- Abstract：Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) and Western blotting were performed to analyze the expression of LSD1 and interleukin-6 (IL-6) in THP-1 monocytes and THP-1-derived macrophages. Chromatin immunoprecipitation (ChIP) assay was applied to detect the occupancy of LSD1 and H3K4 methylation at IL-6 promoter during THP-1 monocyte-to-macrophage differentiation. IL-6 mRNA level and H3K4 methylation at IL-6 promoter were analyzed using qRT-PCR and ChIP assay in LSD1-knockdown THP-1 cells treated with 12-O-tetradecanoylphorbol-13-acetate (TPA) for 0, 4, 8, 12, and 24 hours. Fluorescence activated flow cytometry was performed to reveal the percentage of macrophages differentiated from THP-1 monocytes.Keywords：lysine-specific demethylase 1;interleukin-6;H3K4 methylation;monocyte-to-macrophage differentiation4|2621|0Updated：2024-04-10
- Abstract：Male littermates and testicular feminized (Tfm) mice were randomly separated into 4 experimental groups littermate controls (n=8), Tfm mice (n=7), testosterone-treated Tfm mice (n=8), and Tfm mice treated with testosterone in combination with the aromatase inhibitor anastrazole (n=7). Cardiomyocytes were isolated from mouse left ventricles, the activity of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px), and the amount of malondialdehyde (MDA) were measured using colorimetry method, and expression of p16INK4α and retinoblastoma (Rb) proteins were detected by Western blotting.Keywords：aging;cardiomyocyte;testosterone;androgen receptor4|927|1Updated：2024-04-10
- Abstract：Pregnant women were recruited from September 2009 to February 2010 at the prenatal clinic in Nanjing Maternity and Child Health Care Hospital. Altogether 174 healthy pregnant women without pregnant or obstetric complications or abnormal pregnancy outcomes were enrolled as the gravida group, and 120 healthy non-pregnant women as the control group. BLLs during pregnancy were determined by flame atomic absorption spectroscopy.Keywords：blood lead levels;pregnancy;lead exposure4|1155|0Updated：2024-04-10
- Abstract：The Editor of Chinese Medical Sciences Journal retracts the following article: Zhen Li, Zhong-gao Wang, Bian Ce, Chen Xiu, Han Bing, Gao-feng Hou, Chu Jian, and Cui Qi. Interferon Regulatory Factor-1 Exerts Inhibitory Effect on Neointimal Formation after Vascular Injury. Chin Med Sci J 2009; 24(2): 91-6. PubMed ID: 19618605. The reason was that the first author, Zhen Li, used data contained in this article without permission of Dr. Masatsugu Horiuchi, Department of Molecular Cardiovascular Biology and Pharmacology of Ehime University. The first author admitted personal responsibility for the mistake.4|11|0Updated：2024-04-10
- Abstract：Medical records of 8005 patients, who underwent laparoscopic cholecystectomy in Peking Union Medical College Hospital between June 1993 and June 2011, were reviewed. Patients that pathologically diagnosed as UGC were retrospectively studied in terms of clinical features, preoperative and postoperative diagnosis, surviving period, and complications.Keywords：laparoscopic cholecystectomy;gallbladder neoplasms;carcinoma;frozen sections;survival rate4|2230|3Updated：2024-04-10
- Abstract：Between January 1994 and December 2011, 165 patients with biopsy-proven adenocarcinoma of the pancreatic head were treated in West China Hospital, among whom 93 underwent SPD and 72 had EPD. Complications and survival after the surgery were analyzed retrospectively.Keywords：pancreas;cancer;pancreaticoduodenectomy;standard resection;extended resection4|1997|0Updated：2024-04-10
- Abstract：Diagnostic accuracy was calculated by comparing biopsy diagnosis with de?nitive pathology in 62 patients who underwent frameless stereotactic brain biopsy between January 2008 and December 2010 in Xiamen University Southeast Hospital. Preoperative characteristics and histological diagnosis were reviewed and then information was analysed to identify factors associated with the biopsy not yielding a diagnosis and complications.Keywords：stereotaxis;frameless;biopsy;histology4|1836|0Updated：2024-04-10
- Abstract：Data of the patients receiving open surgical Tenkchoff straight catheter insertion in our department from January 2005 to January 2011 were retrospectively analyzed. The 117 patients in whom PDC insertion was conducted with the guidance of guide wire were enrolled into group A, and the 121 cases receiving PDC insertion without guide wire were enrolled into group B. The incidences of post-operative complications (catheter obstruction, catheter displacement, bloody dialysate, and dialysate leakage), catheter survival, and patient survival rates were compared between the 2 groups. The baseline characteristics (gender, age, body mass index, prothrombin time, activated partial thromboplastin time, platelet count, serum creatinine, follow-up time, primary diseases, and outcomes) of the 2 groups were comparable (all P>0.05). In post-operative complications, only the incidence of early bloody dialysate showed significant difference, being 16.2% in group A and 7.4% in group B (P=0.04). Catheter and patient survival rates were not significantly different between the two groups. Overweight patients showed a higher incidence of catheter obstruction compared with normal weight patients [16.0% (4/25) vs.3.3% (7/213), P=0.02], but no differences in post-operative complications were found among overweight patients between the 2 groups.Keywords：peritoneal dialysis;catheters;surgery;guide wire6|2202|0Updated：2024-04-10
- Abstract：With the development of genome-wide sequencing technology, 195 types of functional long non-coding RNAs (lncRNAs) have so far been found, and their cellular roles are gradually being revealed. Now lncRNAs have become a hotspot in the life science. These small molecules exist in almost all higher eukaryotes, and have very important regulatory roles in these organisms. This review briefly summarizes recent progress in researches on antisense non-coding RNA in the INK4 locus.Keywords：long non-coding RNAs;antisense non-coding RNA in the INK4 locus;expression4|2027|0Updated：2024-04-10
- Abstract：SINCE the introduction of double J stents in 1978, ureteral stents have become an integral tool in treating ureteral obstruction from calculi and other causes.1 Owing to the widespread usage of indwelling ureteral catheters, a number of possible complications such as migration, infection, pyelonephritis, breakage, encrustation, stone formation, and ureteral stents fragmentation have been noted.2 Of all the complications, ureteral stent fragmentation was sporadically reported and believed to be rare.3 Here we present a case of fragmentation of a new type of double J stent, which was used to provide free drainage to the bladder after balloon dilation.Keywords：ureteral stent fragmentation;ureteral foreign body;complication4|2183|0Updated：2024-04-10
- Keywords：chronic myeloid leukemia;imatinib;imatinib-resistent;rapamycin;mammalian target of rapamycin4|883|1Updated：2024-04-10
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