Objective To establish and evaluate a hypercoagulable animal model for the assessment of anticoagulants. Methods Forty mice

thirty-two rats

and twenty-four rabbits were randomly and equally divided into control group (saline) and three ellagic acid (EA)-treated groups (low

middle

and high doses). In the mice

bleeding time (BT) was estimated with tail transaction

and clotting time (CT) with template method. Prothrombin time (PT) and the activated partial thromboplastin time (APTT) in rats and rabbits were measured by means of Quick’s one-stage assay and modified APTT assay respectively. In addition

thrombin activity was estimated in rats with PT assay using a hemagglutination analyzer. The circulating platelet aggregates were detected in rabbits through platelet counting and presented as the circulating platelet aggregate ratio (CPAR). Results EA shortened BT and CT in mice

PT and APTT in rats

and increased thrombin activity and CPAR

all in a dose-dependent manner. EA also brought reduction of PT and APTT in rabbits in dose- and time-dependent manners. Conclusion EA could induce hypercoagulable state through activating coagulation system and platelets in mice

rats

and rabbits.