白细胞介素-36<italic style="font-style: italic">β</italic>对体外培养角质形成细胞炎症反应与分化的影响

王文明; 吴超; 余晓玲; 晋红中

doi:10.24920/003489

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白细胞介素-36β对体外培养角质形成细胞炎症反应与分化的影响

中国医学科学杂志（英文版） 2019年34卷第3期页码：199-204

Original Article | Updated：2024-04-10

- 白细胞介素-36β对体外培养角质形成细胞炎症反应与分化的影响
- Affiliations：
  
  1. 1 Department of Dermatology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100730, China
  2. 2 Department of Dermatology, Dermatology Hospital of Southern Medical University, Guangdong Provincial Dermatology Hospital, Guangdong 510080, China
- Author bio：
  
  *Tel & Fax: 86-10-69151502, E-mail: jinhongzhong@263.net
- Funds：
  
  Supported by the National Natural Science Foundation of China(81773331);CAMS Initiative for Innovative Medicine(No. 2017-I2M-B&R-01)
- DOI：10.24920/003489
  中图分类号：
- 收稿日期：2018-12-28，
  
  网络出版日期：2019-09-30，
  
  纸质出版日期：2019-09-30
- Accepted：
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王文明, 吴超, 余晓玲, 等. 白细胞介素-36β对体外培养角质形成细胞炎症反应与分化的影响[J]. 中国医学科学杂志（英文版）, 2019,34(3):199-204.

Wang Wenming, Wu Chao, Yu Xiaoling, et al. IL-36β Promotes Inflammatory Activity and Inhibits Differentiation of Keratinocytes In Vitro[J]. Chinese medical sciences journal, 2019, 34(3): 199-204.
王文明, 吴超, 余晓玲, 等. 白细胞介素-36β对体外培养角质形成细胞炎症反应与分化的影响[J]. 中国医学科学杂志（英文版）, 2019,34(3):199-204. DOI： 10.24920/003489.

Wang Wenming, Wu Chao, Yu Xiaoling, et al. IL-36β Promotes Inflammatory Activity and Inhibits Differentiation of Keratinocytes In Vitro[J]. Chinese medical sciences journal, 2019, 34(3): 199-204. DOI： 10.24920/003489.

摘要

目的

银屑病是一种免疫介导的炎症性疾病。尽管目前银屑病的研究取得了巨大的进展

但其确切机制尚不完全清楚。表皮的增殖异常在银屑病的发病中发挥重要作用。本研究将在体外探究白细胞介素-36β（IL-36β）对角质形成细胞功能的影响。

方法

体外培养人永生化角质形成细胞系HaCaT

分为3组:对照组、50 ng/ml IL-36

组、100 ng/ml IL-36

组

分别处理24 h。采用CCK8法检测细胞增殖

流式细胞术检测细胞凋亡与细胞周期分布

实时荧光定量PCR检测角质形成细胞分化指标keratin 10 mRNA和involucrin mRNA的表达

ELISA法检测培养液中炎症因子IL-1β和IL-6的含量。

结果

与对照组相比

50、100 ng/ml IL-36

可使HaCaT细胞阻滞在S期（

0.05）;同时可抑制细胞keratin 10、involucrin mRNA的表达（

0.01）;100 ng/ml IL-36

可增加细胞培养液中IL-1

与IL-6的含量（

0.05）。

结论

IL-36

可能导致角质形成细胞周期阻滞

抑制角质形成细胞分化

促进角质形成细胞的炎症反应。

Abstract

Objective

Psoriasis is an immune-mediated inflammatory disease. Despite advances in the study of its pathogenesis

the exact development mechanism of psoriasis remains to be fully elucidated. Hyperproliferative epidermis plays a crucial role in psoriasis. This study aimed to investigate the effects of interleukin-36

(IL-36

) on keratinocyte dysfunction

in vitro

Methods

Human keratinocyte cell lines

HaCaT cells

were treated with 0 (control)

50 or 100 ng/ml IL-36

respectively for 24 h. Cell viability was determined with a cell counting kit-8 assay. Flow cytometry was used to assess the effects of IL-36

on apoptosis and cell cycle distribution. Expressions of the differentiation markers

such as keratin 10 and involucrin

were evaluated by quantitative real-time polymerase chain reaction (RT-qPCR). Expressions of the inflammatory cytokines

IL-1

and IL-6 were tested by ELISA.

Results

CCK8 assay showed the survival rate had no significant difference between the control and treated group (

0.05). Flow cytometry analysis showed cell cycle arrest at S phase in the IL-36

-treated groups compared with the control group (

0.05). RT-qPCR verified the decreased mRNA expressions of keratin 10 and involucrin in the IL-36

-treated groups compared with the negative control (

0.01). ELISA showed 100 ng/ml IL-36

enhanced levels of IL-1

and IL-6 in culture supernatants of HaCaT cells compared with the negative control (

0.05).

Conclusion

Taken together

these findings suggest that IL-36

could induce cell cycle arrest at S phase

inhibit keratin 10 and involucrin expressions and promote inflammatory activity in HaCaT cell lines.

关键词

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references

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白细胞介素-36β对体外培养角质形成细胞炎症反应与分化的影响

DOI：10.24920/003489

摘要

Abstract

关键词

Keywords

references