Alpha-GalCer Administration after Allogeneic Bone Marrow Transplantation Improves Immune Reconstitution in Mice

Jing-hua Liu; Li-ping Dou; Li-xin Wang; Li-li Wang; Fan Zhou; and Li Yu

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Alpha-GalCer Administration after Allogeneic Bone Marrow Transplantation Improves Immune Reconstitution in Mice

中国医学科学杂志（英文版） 2011年第2期页码：91-97

• Original Article • | Updated：2024-04-10

- Alpha-GalCer Administration after Allogeneic Bone Marrow Transplantation Improves Immune Reconstitution in Mice
- Affiliations：
  
  1. Department of Hematology, General Hospital of the Chinese People’s Liberation Army,Beijing,China,100853
  2. Department of Hematology, Shenyang Military General Hospital,Shenyang,China,110016
- Author bio：
- Funds：
- DOI：
  中图分类号：
- 网络出版日期：2011-06-24，
  
  纸质出版日期：2011
- Accepted：
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Jing-hua Liu, Li-ping Dou, Li-xin Wang, 等. Alpha-GalCer Administration after Allogeneic Bone Marrow Transplantation Improves Immune Reconstitution in Mice[J]. 中国医学科学杂志（英文版）, 2011,(2):91-97.

Jing-hua Liu, Li-ping Dou, Li-xin Wang, et al. Alpha-GalCer Administration after Allogeneic Bone Marrow Transplantation Improves Immune Reconstitution in Mice[J]. Chinese medical sciences journal, 2011, (2): 91-97.
Jing-hua Liu, Li-ping Dou, Li-xin Wang, 等. Alpha-GalCer Administration after Allogeneic Bone Marrow Transplantation Improves Immune Reconstitution in Mice[J]. 中国医学科学杂志（英文版）, 2011,(2):91-97. DOI：

Jing-hua Liu, Li-ping Dou, Li-xin Wang, et al. Alpha-GalCer Administration after Allogeneic Bone Marrow Transplantation Improves Immune Reconstitution in Mice[J]. Chinese medical sciences journal, 2011, (2): 91-97. DOI：

摘要

Objective To explore the effect of α- galactosyleramide( α-GalCer ) on immune reconstitution under acute graft-versus-host disease (aGVHD). Methods BALB/c mice were transplanted wit hallogeneic C57BL/6 bone marrow cells and splenocytes (both 1×10

)after receiving lethal total-body irradiation. α-GalCer (100 ug/kg) or vehicle (dimethylsulfoxide) was administered intraperitoneally immediately after transplantation. The effects of α-GalCer on immune reconstitution

proliferation of T cells and B cells

hematopoiesis

and thymic microenvironment were assessed. Results The α-GalCer group exhibited higher percentages of CD3

CD4

CD8

B220

CD40

and CD86

cells compared with the vehicle group . The number of colony forming unit per 1000 CD34

cells in the α-GalCer group was higher than in the vehicle group ( P=0.0012).In vitro proliferation assays showed that the α-GalCer group had higher percentages of CD3

CD4

CD8

and B220

cells compared with the vehicle group. As for the results of in vivo proliferation assays

the numbers of CD3

CD4

CD8

and B220

cells were higher in the α-GalCer group than in the normal group

especially the number of B220

cells ( P=0.007).Significant difference was not found in thymocyte count between the α-GalCer group and the vehicle group

nor in the percentages of CD3

CD4

and CD8

cells.Conclusion Administration of α-GalCer after allogeneic bone marrow transplantation may promote immune reconstitution in the presence of aGVHD.

Abstract

proliferation of T cells and B cells

hematopoiesis

and thymic microenvironment were assessed. Results The α-GalCer group exhibited higher percentages of CD3

CD4

CD8

B220

CD40

and CD86

cells compared with the vehicle group . The number of colony forming unit per 1000 CD34

cells in the α-GalCer group was higher than in the vehicle group ( P=0.0012).In vitro proliferation assays showed that the α-GalCer group had higher percentages of CD3

CD4

CD8

and B220

cells compared with the vehicle group. As for the results of in vivo proliferation assays

the numbers of CD3

CD4

CD8

and B220

cells were higher in the α-GalCer group than in the normal group

especially the number of B220

cells ( P=0.007).Significant difference was not found in thymocyte count between the α-GalCer group and the vehicle group

nor in the percentages of CD3

CD4

and CD8

cells.Conclusion Administration of α-GalCer after allogeneic bone marrow transplantation may promote immune reconstitution in the presence of aGVHD.

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