PTEN and PDCD4 are Bona Fide Targets of microRNA-21 in Human Cholangiocarcinoma

Chang-zheng Liu; Wei Liu; Yi Zheng; Jin-mei Su; Jing-jing Li; Lan Yu; Xiao-dong He; and Song-sen Chen

Your Location：

Home >

Browse articles >

PTEN and PDCD4 are Bona Fide Targets of microRNA-21 in Human Cholangiocarcinoma

中国医学科学杂志（英文版） 2012年27卷第2期页码：65-72

Updated：2024-04-10

- PTEN and PDCD4 are Bona Fide Targets of microRNA-21 in Human Cholangiocarcinoma
- Affiliations：
  
  1. Department of Biochemistry, National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences & Peking Union Medical College,Beijing,China,100005
  2. Department of General Surgery
  3. Department of Rheumatology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College,Beijing,China,100730
- Author bio：
- Funds：
- DOI：
  中图分类号：
- 网络出版日期：2012-07-05，
  
  纸质出版日期：2012
- Accepted：
Scan QR Code
Chang-zheng Liu, Wei Liu, Yi Zheng, 等. PTEN and PDCD4 are Bona Fide Targets of microRNA-21 in Human Cholangiocarcinoma[J]. 中国医学科学杂志（英文版）, 2012,27(2):65-72.
Chang-zheng Liu, Wei Liu, Yi Zheng, 等. PTEN and PDCD4 are Bona Fide Targets of microRNA-21 in Human Cholangiocarcinoma[J]. 中国医学科学杂志（英文版）, 2012,27(2):65-72. DOI：

DOI：

摘要

To investigate the expression profile of microRNA-21 in human cholangiocarcinoma tissues and to validate its bona fide targets in human cholangiocarcinoma cells. The expression profile of microRNA-21 in human cholangiocarcinoma tissues and cholangiocarcinoma cell line

QBC939

was evaluated by using real-time PCR analysis. The bona fide targets of microRNA-21 were analyzed and confirmed by dual luciferase reporter gene assay and western blot

respectively. The expressional correlation of microRNA-21 and its targets was probed in human cholangiocarcinoma tissues by using real-time PCR

locked nucleic acid hybridization (LNA-ISH)

and immunohistochemistry analysis. Real-time PCR analysis revealed that microRNA-21 expression depicted a significant up-regulation in human cholangiocarcinoma tissues about 5.6-fold as compared to the matched normal bile Conclusion microRNA-21 expression is up-regulated in human cholangiocarcinoma and PTEN

PDCD4 are direct effectors of microRNA-21. △Supported by Institute of Basic Medical Sciences (2009PY13 and 2010PYZ18) and the National Natural Science Foundation of China (81100608 and 30901342).

Abstract

关键词

Keywords

references

浏览量

4039

Downloads

CSCD

文章被引用时，请邮件提醒。

Submit

关联资源

PTEN and PDCD4 are Bona Fide Targets of microRNA-21 in Human Cholangiocarcinoma^△

Influence of Photodynamic Therapy on Apoptosis and Invasion of Human Cholangiocarcinoma QBC939 Cell Line