Zinc Finger Protein-activating Transcription Factor Up-regulates Vascular Endothelial Growth Factor-A Expression in Vitro△

Li-shan Lian; Yao-guo Yang; Wei Liu; Li-long Guo; Heng Guan; Chang-wei Liu; Yong-jun Li

Your Location：

Home >

Browse articles >

Zinc Finger Protein-activating Transcription Factor Up-regulates Vascular Endothelial Growth Factor-A Expression in Vitro△

中国医学科学杂志（英文版） 2012年27卷第3期页码：171-175

• Original Article • | Updated：2024-04-10

- Zinc Finger Protein-activating Transcription Factor Up-regulates Vascular Endothelial Growth Factor-A Expression in Vitro△
- Affiliations：
  
  Department of Vascular Surgery, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College,Beijing,China,100730
- Author bio：
- Funds：
- DOI：
  中图分类号：
- 网络出版日期：2012-10-08，
  
  纸质出版日期：2012
- Accepted：
Scan QR Code
Li-shan Lian, Yao-guo Yang, Wei Liu, 等. Zinc Finger Protein-activating Transcription Factor Up-regulates Vascular Endothelial Growth Factor-A Expression in Vitro△[J]. 中国医学科学杂志（英文版）, 2012,27(3):171-175.

Li-shan Lian, Yao-guo Yang, Wei Liu, et al. Zinc Finger Protein-activating Transcription Factor Up-regulates Vascular Endothelial Growth Factor-A Expression in Vitro△[J]. Chinese medical sciences journal, 2012, 27(3): 171-175.
Li-shan Lian, Yao-guo Yang, Wei Liu, 等. Zinc Finger Protein-activating Transcription Factor Up-regulates Vascular Endothelial Growth Factor-A Expression in Vitro△[J]. 中国医学科学杂志（英文版）, 2012,27(3):171-175. DOI：

Li-shan Lian, Yao-guo Yang, Wei Liu, et al. Zinc Finger Protein-activating Transcription Factor Up-regulates Vascular Endothelial Growth Factor-A Expression in Vitro△[J]. Chinese medical sciences journal, 2012, 27(3): 171-175. DOI：

摘要

To construct the zinc finger protein-activating transcription factor (ZFP-ATF) plasmid and evaluate its efficacy in inducing vascular endothelial growth factor (VEGF) expression in EY.HY926 endothelial cells. Methods Firstly

we constructed the ZFP-ATF plasmid

then testified the quantity of VEGF protein in EY.HY926 endothelial cells after transfected with ZFP-ATP plasmid by Western blot

finally

we used the RT-PCR to testify whether the ZFP-ATF can stimulate expression of VEGF splice variants. Results The ZFP-ATF DNA sequences were located the multiclone sites of PVAX1 vector between the site of BamHⅠand Xhol.Western blot result showed VEGF expression in EY.HY926 endothelial cells transfected with ZFP-ATF plasmid was significantly higher than that in cells transfected with VEGF165 (19.95±3.95 vs.12.15±1.55 μg/μL

P<0.01).RT-PCR result showed VEGF-A mRNA expression level induced by ZFP-ATF was high than that induced by VEGF165. Conclusion ZFP-ATF can up-regulate the VEGF-A expression in comparison with VEGF165

which might have beneficial effects in angiogenesis process.

Abstract

we constructed the ZFP-ATF plasmid

then testified the quantity of VEGF protein in EY.HY926 endothelial cells after transfected with ZFP-ATP plasmid by Western blot

finally

P<0.01).RT-PCR result showed VEGF-A mRNA expression level induced by ZFP-ATF was high than that induced by VEGF165. Conclusion ZFP-ATF can up-regulate the VEGF-A expression in comparison with VEGF165

which might have beneficial effects in angiogenesis process.

关键词

Keywords

references

浏览量

2477

Downloads

CSCD

文章被引用时，请邮件提醒。

Submit

关联资源

REGULATING EFFECTS OF VASCULAR ENDOTHELIAL GROWTH FACTOR AND ANG Ⅱ ON FROG'S PERICARDIAL STOMATA, MESOTHELIUM AND ANGIOGENESIS

THE EFFECT OF BASIC FIBROBLAST GROWTH FACTOR SLOW-RELEASE MICROCAPSULES ON ANGIOGENESIS IN INFARCTED RABBIT MYOCARDIUM

CONTROL OF ANGIOGENESIS BY INHIBITOR OF PHOSPHOLIPASE A₂

Serum HIF-1α and VEGF Levels Pre- and Post-TACE in Patients with Primary Liver Cancer