ObjectiveTo screen the asymmetric dimethyl arginines (ADMA)-containing proteins which could combine with protein arginine methyltransferase 1 (PRMT1). Methods Western blot was adopted to identify the expression of PRMT1 and the proteins with ADMA in glioma cell lines and normal brain tissues

and then to detect the changes of ADMA level after knock-down of PRMT1 with RNAi transfection in U87MG cells. Co-Immunoprecipitation (Co-IP)

western blot

and sliver staining were employed to screen the candidate binding proteins of PRMT1. Then liquid chromatography-tandem mass spectrometry (LC-MS/MS) was used to identify the binding proteins of PRMT1. Results The expression of PRMT1 and some levels of ADMA were higher in glioma cell lines than in normal brain tissues. After knocking down PRMT1

some ADMA levels were found declined. After screening the binding proteins of PRMT1 with Co-IP and LC-MS/MS

26 candidate binding proteins were identified. Among them

6 candidate proteins had higher ions scores (>38) and bioinformation analysis predicted that SEC23-IP

ANKHD1-EIF4EBP3 protein

and 1-phosphatidylinositol-3-phosphate 5-kinase isoform 2 had possible methylated aginine sites. Conclusions The high expression of PRMT 1 in glioma may induce the change of ADMA levels. Altogether 26 candidate proteins were identified

which contain ADMA and specifically bind with PRMT1.