Phosphatase and Tension Homolog Overexpression in Insulin Resistant Diabetic Adipose Tissue<sup>△</sup>

Jing-bo Zeng; Yun Zhang; Qi Sun; Yu-xiu Li

Your Location：

Home >

Browse articles >

Phosphatase and Tension Homolog Overexpression in Insulin Resistant Diabetic Adipose Tissue^△

中国医学科学杂志（英文版） 2014年29卷第3期页码：167-173

• Orginal Article • | Updated：2024-04-10

- Phosphatase and Tension Homolog Overexpression in Insulin Resistant Diabetic Adipose Tissue^△
- Affiliations：
  
  1. Department of Endocrinology, Key Laboratory of Endocrinology of the Ministry of Health, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College,Beijing,China,100730
  2. ,China
  3. Department of Endocrinology, Fuxing Hospital Affiliated to Capital University of Medical Sciences,Beijing,China,100038
- Author bio：
- Funds：
- DOI：
  中图分类号：
- 网络出版日期：2014-09-29，
  
  纸质出版日期：2014
- Accepted：
Scan QR Code
Jing-bo Zeng, Yun Zhang, Qi Sun, 等. Phosphatase and Tension Homolog Overexpression in Insulin Resistant Diabetic Adipose Tissue^△[J]. 中国医学科学杂志（英文版）, 2014,29(3):167-173.

Jing-bo Zeng, Yun Zhang, Qi Sun, et al. Phosphatase and Tension Homolog Overexpression in Insulin Resistant Diabetic Adipose Tissue[J]. Chinese medical sciences journal, 2014, 29(3): 167-173.
Jing-bo Zeng, Yun Zhang, Qi Sun, 等. Phosphatase and Tension Homolog Overexpression in Insulin Resistant Diabetic Adipose Tissue^△[J]. 中国医学科学杂志（英文版）, 2014,29(3):167-173. DOI：

Jing-bo Zeng, Yun Zhang, Qi Sun, et al. Phosphatase and Tension Homolog Overexpression in Insulin Resistant Diabetic Adipose Tissue[J]. Chinese medical sciences journal, 2014, 29(3): 167-173. DOI：

摘要

Objective To investigate the expression of phosphatase and tension homolog (PTEN) in adipose tissue of KKAy diabetic mice

a mouse model of type 2 diabetes. Methods KKAy diabetic mice were fed with high fat diet for 4 weeks. After blood glucose met the criteria of diabetes (over 16.7 mmol/L)

mice were randomly divided into 3 groups: a control group (without any treatment)

a rosiglitazone group (treated with rosiglitazone 12.5 mg/kg?d once per day)

and a metformin group (treated with metformin 3 g/kg?d twice daily). After 4 weeks

we then determined the expression of PTEN and phosphoserine 473-Akt (pS473-Akt) in the epididymal adipose tissue with Western blots. The mice in each group were further divided into the insulin (-) subgroup and insulin (+) subgroup

which were intraperitoneally injected with saline and insulin (5 mU/g body weight)

respectively. Results The expression of PTEN was elevated in the epididymal adipose tissue obtained from KKAy diabetic mice compared with that from the C57BL/6J mice (P<0.001). In accordance with the enhanced expression of PTEN

the level of pS473-Akt stimulated by insulin was decreased in the adipose tissue of KKAy mice compared to the C57BL/6J mice (P<0.001). Treatment with the insulin-sensitizing agents

rosiglitazone and metformin did not inhibit the elevated expression of PTEN in adipose tissue of KKAy diabetic mice. Conclusion PTEN may play an important role in the development of insulin resistance in adipose tissue of type 2 diabetes mice model.

Abstract

Objective To investigate the expression of phosphatase and tension homolog (PTEN) in adipose tissue of KKAy diabetic mice

a mouse model of type 2 diabetes. Methods KKAy diabetic mice were fed with high fat diet for 4 weeks. After blood glucose met the criteria of diabetes (over 16.7 mmol/L)

mice were randomly divided into 3 groups: a control group (without any treatment)

a rosiglitazone group (treated with rosiglitazone 12.5 mg/kg?d once per day)

and a metformin group (treated with metformin 3 g/kg?d twice daily). After 4 weeks

which were intraperitoneally injected with saline and insulin (5 mU/g body weight)

the level of pS473-Akt stimulated by insulin was decreased in the adipose tissue of KKAy mice compared to the C57BL/6J mice (P<0.001). Treatment with the insulin-sensitizing agents

关键词

Keywords

references

浏览量

961

Downloads

CSCD

文章被引用时，请邮件提醒。

Submit

关联资源

INSULIN RESISTANCE AND CAROTID ATHEROSCLEROSIS IN 221 PATIENTS WITH POTENTIAL HYPERGLYCEMIA

PLASMA RESISTIN LEVELS AND SINGLE-NUCLEOTIDE POLYMORPHISMS IN RESISTIN GENE 5 FLANKING REGION IN PATIENTS WITH STROKE

Fatty Acid Synthase and Hormone-sensitive Lipase Expression in Liver Are Involved in Zinc-α2-glycoprotein-induced Body Fat Loss in Obese Mice

Nucleotide-binding Oligomerization Domain-1 Ligand Induces Inflammation and Attenuates Glucose Uptake in Human Adipocytes△

Saturated Fatty Acid Induces Insulin Resistance Partially Through Nucleotide-binding Oligomerization Domain 1 Signaling Pathway in Adipocytes^△