Ribotrap Analysis of Proteins Associated with FHL3 3’Untranslated Region in Glioma Cells<sup>△</sup>

Wei Han; Qing Xia; Bin Yin; Xiao-zhong Peng

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Ribotrap Analysis of Proteins Associated with FHL3 3’Untranslated Region in Glioma Cells^△

中国医学科学杂志（英文版） 2014年29卷第2期页码：78-84

• 论著 • | Updated：2024-04-10

- Ribotrap Analysis of Proteins Associated with FHL3 3’Untranslated Region in Glioma Cells^△
- Affiliations：
  
  1. State Key Laboratory of Medical Molecular Biology, Department of Biochemistry and Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences & Peking Union Medical College,Beijing,China,100005
  2. ,China
  3. State Key Laboratory of Proteomics, Institute of Basic Medical Sciences, National Center of Biomedical Analysis,Beijing,China,100850
- Author bio：
- Funds：
- DOI：
  中图分类号：
- 网络出版日期：2014-06-26，
  
  纸质出版日期：2014
- Accepted：
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Wei Han, Qing Xia, Bin Yin, 等. Ribotrap Analysis of Proteins Associated with FHL3 3’Untranslated Region in Glioma Cells^△[J]. 中国医学科学杂志（英文版）, 2014,29(2):78-84.

Wei Han, Qing Xia, Bin Yin, et al. Ribotrap Analysis of Proteins Associated with FHL3 3’Untranslated Region in Glioma Cells[J]. Chinese medical sciences journal, 2014, 29(2): 78-84.
Wei Han, Qing Xia, Bin Yin, 等. Ribotrap Analysis of Proteins Associated with FHL3 3’Untranslated Region in Glioma Cells^△[J]. 中国医学科学杂志（英文版）, 2014,29(2):78-84. DOI：

Wei Han, Qing Xia, Bin Yin, et al. Ribotrap Analysis of Proteins Associated with FHL3 3’Untranslated Region in Glioma Cells[J]. Chinese medical sciences journal, 2014, 29(2): 78-84. DOI：

摘要

Objective To screen the proteins associated with four-and-a-half LIM domains 3 (FHL3) 3’ untranslated region (3’UTR) in glioma cells.Methods Western blot was adopted to detect the regulatory effect of poly(C)-binding protein 2 (PCBP2) on FHL3. Biotin pull-down and sliver staining were employed to screen and verify the candidate binding proteins of FHL3 3’UTR. Then liquid chromatography-tandem mass spectrometry (LC-MS/MS) and molecule annotation system were used to identify and analyze the candidate binding proteins. Immuno- precipitation was conducted to study the interaction between PCBP2 and polypyrimidine tract-binding protein 1 (PTBP1)

a binding protein identified by LC-MS/MS.Results PCBP2 could bind to FHL3 mRNA 3’UTR-A and inhibited the expression of FHL3 in T98G glioms cells. 22 candidate binding proteins were identified. Among them

there were 11 RNA binding proteins

including PCBP2. PTBP1 associated with FHL3 mRNA 3’UTR and interacted with PCBP2 protein.Conclusions PCBP2 and PTBP1 can both associate with FHL3 mRNA 3’UTR through forming a protein complex.

Abstract

there were 11 RNA binding proteins

including PCBP2. PTBP1 associated with FHL3 mRNA 3’UTR and interacted with PCBP2 protein.Conclusions PCBP2 and PTBP1 can both associate with FHL3 mRNA 3’UTR through forming a protein complex.

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