PTEN and PDCD4 are Bona Fide Targets of microRNA-21 in Human Cholangiocarcinoma<sup>△</sup>

Chang-zheng Liu; Wei Liu; Yi Zheng; Jin-mei Su; Jing-jing Li; Lan Yu; Xiao-dong He; and Song-sen Chen

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PTEN and PDCD4 are Bona Fide Targets of microRNA-21 in Human Cholangiocarcinoma^△

中国医学科学杂志（英文版） 2012年第2期页码：65-72

• Original Article • | Updated：2024-04-10

- PTEN and PDCD4 are Bona Fide Targets of microRNA-21 in Human Cholangiocarcinoma^△
- Affiliations：
  
  1. Department of Biochemistry, National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences & Peking Union Medical College,Beijing,China,100005
  2. Department of General Surgery
  3. Department of Rheumatology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College,Beijing,China,100730
- Author bio：
  
  [ "†These authors equally contribute to this work.*Corresponding author Chang-zheng Liu, Tel: 86-10-69156424, Fax: 86-10-65253005, E-mail: cz-liu@ibms.pumc.edu.cn" ]
- Funds：
- DOI：
  中图分类号：
- 网络出版日期：2012-07-05，
  
  纸质出版日期：2012
- Accepted：
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Chang-zheng Liu, Wei Liu, Yi Zheng, 等. PTEN and PDCD4 are Bona Fide Targets of microRNA-21 in Human Cholangiocarcinoma^△[J]. 中国医学科学杂志（英文版）, 2012,(2):65-72.

Chang-zheng Liu, Wei Liu, Yi Zheng, et al. PTEN and PDCD4 are Bona Fide Targets of microRNA-21 in Human Cholangiocarcinoma[J]. Null, 2012, (2): 65-72.
Chang-zheng Liu, Wei Liu, Yi Zheng, 等. PTEN and PDCD4 are Bona Fide Targets of microRNA-21 in Human Cholangiocarcinoma^△[J]. 中国医学科学杂志（英文版）, 2012,(2):65-72. DOI：

Chang-zheng Liu, Wei Liu, Yi Zheng, et al. PTEN and PDCD4 are Bona Fide Targets of microRNA-21 in Human Cholangiocarcinoma[J]. Null, 2012, (2): 65-72. DOI：

摘要

Objective To investigate the expression profile of microRNA-21 in human cholangiocarcinoma tissues and to validate its bona fide targets in human cholangiocarcinoma cells. Methods The expression profile of microRNA-21 in human cholangiocarcinoma tissues and cholangiocarcinoma cell line

QBC939

was evaluated by using real-time PCR analysis. The bona fide targets of microRNA-21 were analyzed and confirmed by dual luciferase reporter gene assay and western blot

respectively. The expressional correlation of microRNA-21 and its targets was probed in human cholangiocarcinoma tissues by using real-time PCR

locked nucleic acid in situ hybridization (LNA-ISH)

and immunohistochemistry analysis. Results Real-time PCR analysis revealed that microRNA-21 expression depicted a significant up-regulation in human cholangiocarcinoma tissues about 5.6-fold as compared to the matched normal bile Conclusion microRNA-21 expression is up-regulated in human cholangiocarcinoma and PTEN

PDCD4 are direct effectors of microRNA-21.

Abstract

QBC939

was evaluated by using real-time PCR analysis. The bona fide targets of microRNA-21 were analyzed and confirmed by dual luciferase reporter gene assay and western blot

respectively. The expressional correlation of microRNA-21 and its targets was probed in human cholangiocarcinoma tissues by using real-time PCR

locked nucleic acid in situ hybridization (LNA-ISH)

PDCD4 are direct effectors of microRNA-21.

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