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Effect of Atorvastatin on Expression of Peroxisome Proliferator-activated Receptor Beta/delta in Angiotensin Ⅱ-induced Hypertrophic Myocardial Cells
In Vitro △- Affiliations：
1Department of Geriatric Cardiology, Chinese PLA General Hospital, Beijing 100853, China
2Department of Pharmacology & Toxicology, Beijing Institute of Radiation Medicine, Beijing 100850, China;
- Author bio：
*Corresponding author Tel: 86-10-88626575, Fax: 86-10-88270497, E-mail: yeping301@yahoo.com.cn
- Funds：△Supported by the National Basic Research Program (973 Program) (2013CB530804).
DOI：
中图分类号：-
网络出版日期：2015-08-20，
纸质出版日期：2015-08
- Accepted：
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Effect of Atorvastatin on Expression of Peroxisome Proliferator-activated Receptor Beta/delta in Angiotensin Ⅱ-induced Hypertrophic Myocardial Cells
In Vitro △[J]. 中国医学科学杂志（英文版）, 2015,30(4):245-251.Sheng Li, Yang Xu, Ye Ping, et al. Effect of Atorvastatin on Expression of Peroxisome Proliferator-activated Receptor Beta/delta in Angiotensin Ⅱ-induced Hypertrophic Myocardial Cells
In Vitro △[J]. Chinese medical sciences journal, 2015, 30(4): 245-251.Effect of Atorvastatin on Expression of Peroxisome Proliferator-activated Receptor Beta/delta in Angiotensin Ⅱ-induced Hypertrophic Myocardial Cells
In Vitro △[J]. 中国医学科学杂志（英文版）, 2015,30(4):245-251. DOI：Sheng Li, Yang Xu, Ye Ping, et al. Effect of Atorvastatin on Expression of Peroxisome Proliferator-activated Receptor Beta/delta in Angiotensin Ⅱ-induced Hypertrophic Myocardial Cells
In Vitro △[J]. Chinese medical sciences journal, 2015, 30(4): 245-251. DOI：
摘要
Abstract
Objective
To explore the effect of atorvastatin on cardiac hypertrophy and to determine the potential mechanism involved.
Methods
An
in vitro
cardiomyocyte hypertrophy from neonatal rats was induced with angiotensinⅡ (Ang Ⅱ) stimulation. Before AngⅡ stimulation
the cultured rat cardiac myocytes were pretreated with atorvastatin at different concentrations (0.1
1
and 10 μmol/L). The following parameters were evaluated: the myocyte surface area
3
H-leucine incorporation into myocytes
mRNA expressions of atrial natriuretic peptide
brain natriuretic peptide
matrix metalloproteinase 9
matrix metalloproteinase 2
and interleukin-1β
mRNA and protein expressions of the δ/β peroxisome proliferator-activated receptor (PPAR) subtypes.
Results
It was shown that atorvastatin could ameliorate Ang Ⅱ-induced neonatal cardiomyocyte hypertrophy in the area of cardiomyocytes
3
H-leucine incorporation
and the expression of atrial natriuretic peptide and brain natriuretic peptide markedly. Meanwhile
atorvastatin also inhibited the augmented mRNA level of several cytokines in hypertrophic myocytes. Furthermore
the down-regulated expression of PPAR- δ/β at both the mRNA and protein levels in hypertrophic myocytes could be significantly reversed by atorvastatin treatment.
Conclusions
Atorvastatin could improve AngⅡ-induced cardiac hypertrophy and inhibit the expression of cytokines. Such effect might be partly achieved through activation of the PPAR-δ/β pathway.
关键词
Keywords
references
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