Expression of miRNA-140 in Chondrocytes and Synovial Fluid of Knee Joints in Patients with Osteoarthritis<sup>△</sup>

Si Hai-bo; Zeng Yi; Zhou Zong-ke; Pei Fu-xing; Lu Yan-rong; Cheng Jing-qiu; Shen Bin

Your Location：

Home >

Browse articles >

Expression of miRNA-140 in Chondrocytes and Synovial Fluid of Knee Joints in Patients with Osteoarthritis^△

中国医学科学杂志（英文版） 2016年31卷第4期页码：207-212

Orginal Article | Updated：2024-04-10

- Expression of miRNA-140 in Chondrocytes and Synovial Fluid of Knee Joints in Patients with Osteoarthritis^△
- Affiliations：
  
  1. 1Department of Orthopedics,
  2. 2 Key Laboratory of Transplant Engineering and Immunology, Regenerative Medicine Research Centre, West China Hospital, Sichuan University, Chengdu 610041, China
- Author bio：
  
  *Corresponding author, Tel: 86-13881878767, E-mail: shenbin_1971@163.com
- Funds：
  
  △Supported by the National Natural Science Foundation of China (No. 81672219;No. 81601936), and the Science and Technology Support Program of Sichuan province (No. 2014SZ0023-2).
- DOI：
  中图分类号：
- 网络出版日期：2016-08-20，
  
  纸质出版日期：2016-08
- Accepted：
Scan QR Code
Expression of miRNA-140 in Chondrocytes and Synovial Fluid of Knee Joints in Patients with Osteoarthritis^△[J]. 中国医学科学杂志（英文版）, 2016,31(4):207-212.

Si Hai-bo, Zeng Yi, Zhou Zong-ke, et al. Expression of miRNA-140 in Chondrocytes and Synovial Fluid of Knee Joints in Patients with Osteoarthritis^△[J]. Chinese medical sciences journal, 2016, 31(4): 207-212.
Expression of miRNA-140 in Chondrocytes and Synovial Fluid of Knee Joints in Patients with Osteoarthritis^△[J]. 中国医学科学杂志（英文版）, 2016,31(4):207-212. DOI：

Si Hai-bo, Zeng Yi, Zhou Zong-ke, et al. Expression of miRNA-140 in Chondrocytes and Synovial Fluid of Knee Joints in Patients with Osteoarthritis^△[J]. Chinese medical sciences journal, 2016, 31(4): 207-212. DOI：

摘要

Abstract

Objective

To investigate the expression of miRNA-140 in chondrocytes and synovial fluid of osteoarthritis (OA) patients

and explore the relationship between the miRNA-140 expression and OA severity.

Methods

This study enrolled 30 OA patients who underwent total knee arthroplasty for chondrocytes sampling and 30 OA patients who underwent intra-articular injection for synovial fluid sampling. All OA patients were grouped into mild [Kellgren and Lawrence (KL) grade 1-2]

moderate (KL grade 3) and severe (KL grade 4)

with 10 in each subgroups for each sampling purposes. 7 non-OA patients and 10 patients with knee injury were collected for cartilage and synovial fluid sampling respectively as control groups. Chondrocytes were isolated from the cartilage tissue and cultured

in vitro

. Quantitative real time PCR for miRNA-140 in chondrocytes and synovial fluid were performed

and the U6 snRNA was used as internal control. The expression difference of miRNA-140 among groups and correlation between the expression and the KL grade of OA were analysed using one-way ANOVA and Spearman test respectively.

Results

The expression of miRNA-140 in chondrocytes of knees in OA patients was reduced than that in normal knees

and the between-group difference was statistically significant (

=305.464

0.001). miRNA-140 could be detected in synovial fluid of both normal knees and OA knees

its relative expression level was reduced in synovial fluid of OA group compared with normal group

and the between-group difference was statistically significant as well (

=314.245

0.001). The relative expression level of miRNA-140 in both chondrocytes and synovial fluid were negatively correlated with the KL grade of OA(

=-0.969

0.001;

=-0.970

0.001).

Conclusion

miRNA-140 could be detected in chondrocytes and synovial fluid of OA patients

and its expression was negatively correlated with the severity of OA.

关键词

Keywords

references

Sharma L. Osteoarthritis year in review 2015: clinical. Osteoarthritis Cartil 2016 ; 24 : 36 - 48 .

Poulet B , Staines KA. New developments in osteoarthritis and cartilage biology. Curr Opin Pharmacol 2016 ; 28 : 8 - 13 .

Xia B , Di Chen , Zhang J , et al. Osteoarthritis pathogenesis: a review of molecular mechanisms. Calcif Tissue Int 2014 ; 95 : 495 - 505 .

Zhang R , Ma J , Yao J. Molecular mechanisms of the cartilage-specific microRNA-140 in osteoarthritis. Inflamm Res 2013 ; 62 : 871 - 7 .

Trzeciak T , Czarny-Ratajczak M. MicroRNAs: Important epigenetic regulators in osteoarthritis. Curr Genomics 2014 ; 15 : 481 - 4 .

Miyaki S , Nakasa T , Otsuki S , et al. MicroRNA-140 is expressed in differentiated human articular chondrocytes and modulates interleukin-1 responses. Arthritis Rheum 2009 ; 60 : 2723 - 30 .

Kellgren JH , Lawrence JS. Radiological assessment of osteo-arthrosis. Ann Rheum Dis 1957 ; 16 : 494 - 502 .

Livak KJ , Schmittgen TD. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. Methods 2001 ; 25 : 402 - 8 .

Araldi E , Schipani E. MicroRNA-140 and the silencing of osteoarthritis. Genes Dev 2010 ; 24 : 1075 - 80 .

Nugent M. MicroRNAs: exploring new horizons in osteoar- thritis. Osteoarthr Cartil 2016 ; 24 : 573 - 80 .

Nakamura Y , He X , Kobayashi T , et al. Unique roles of microRNA140 and its host gene WWP2 in cartilage biology. J Musculoskelet Neuronal Interact 2008 ; 8 : 321 - 2 .

Yang J , Qin S , Yi C , et al. MiR-140 is co-expressed with Wwp2-C transcript and activated by Sox9 to target Sp1 in maintaining the chondrocyte proliferation. FEBS Lett 2011 ; 585 : 2992 - 7 .

Asahara H . miRNAs in cartilage development. Clin Calcium 2012 ; 22 : 653 - 7 .

Swingler TE , Wheeler G , Carmont V , et al. The expression and function of microRNAs in chondrogenesis and osteoar- thritis. Arthritis Rheum 2012 ; 64 : 1909 - 19 .

Goldring MB. Update on the biology of the chondrocyte and new approaches to treating cartilage diseases. Best Pract Res Clin Rheumatol 2006 ; 20 : 1003 - 25 .

Blasioli DJ , Kaplan DL. The roles of catabolic factors in the development of osteoarthritis. Tissue Eng Part B Rev 2014 ; 20 : 355 - 63 .

Scanzello CR , Goldring SR. The role of synovitis in osteoarthritis pathogenesis. Bone 2012 ; 51 : 249 - 57 .

Shoji T , Nakasa T , Yamasaki K , et al. The effect of intra- articular injection of microRNA-210 on ligament healing in a rat model. Am J Sports Med 2012 ; 40 : 2470 - 8 .

Kawanishi Y , Nakasa T , Shoji T , et al. Intra-articular injection of synthetic microRNA-210 accelerates avascular meniscal healing in rat medial meniscal injured model. Arthritis Res Ther 2014 ; 16 : 488 .

浏览量

Downloads

CSCD

文章被引用时，请邮件提醒。

Submit

关联资源

暂无数据