Up-regulation of <italic style="font-style: italic">Let-7a</italic> Expression Induces Gastric Carcinoma Cell Apoptosis <italic style="font-style: italic">In Vitro</italic>

Zhu Yimin; Xu Fuying

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Up-regulation of Let-7a Expression Induces Gastric Carcinoma Cell Apoptosis In Vitro

中国医学科学杂志（英文版） 2017年32卷第1期页码：44-47

Orginal Article | Updated：2024-04-10

- Up-regulation of Let-7a Expression Induces Gastric Carcinoma Cell Apoptosis In Vitro
- Affiliations：
  
  Department of Surgery, College of Medicine and Health, Lishui University/Lishui People’s Hospital, Lishui, Zhejiang 323000, China
- Author bio：
  
  *Corresponding author E-mail: zym330422@163.com
- Funds：
- DOI：
  中图分类号：
- 网络出版日期：2017-04-11，
  
  纸质出版日期：2017-04
- Accepted：
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Up-regulation of Let-7a Expression Induces Gastric Carcinoma Cell Apoptosis In Vitro[J]. 中国医学科学杂志（英文版）, 2017,32(1):44-47.

Zhu Yimin, Xu Fuying. Up-regulation of Let-7a Expression Induces Gastric Carcinoma Cell Apoptosis In Vitro[J]. Chinese medical sciences journal, 2017, 32(1): 44-47.
Up-regulation of Let-7a Expression Induces Gastric Carcinoma Cell Apoptosis In Vitro[J]. 中国医学科学杂志（英文版）, 2017,32(1):44-47. DOI：

Zhu Yimin, Xu Fuying. Up-regulation of Let-7a Expression Induces Gastric Carcinoma Cell Apoptosis In Vitro[J]. Chinese medical sciences journal, 2017, 32(1): 44-47. DOI：

摘要

Abstract

Objective

To investigate effect of

let-7a

gene over-expression on apoptosis of gastric cancer cell lines SGC-7901.

Methods

The stable

let-7a

gene over-expressing SGC-7901 cells

SGC-7901/

let-7a

cells

were established using shRNA lentiviral vector methods. Real-time RT-PCR analysis was used to evaluate the expression level of let-7a mRNA. Cells apoptosis was assessed by flow cytometry.

Results

RT-PCR analysis revealed

let-7a

expression in SGC-7901/

let-7a

cells was significantly increased. Cellular apoptosis assay showed that over-expression of

let-7a

could increase apoptosis of SGC-7901 cells (

0.002).

Conclusion

Up-regulating

let-7a

expression promoted apoptosis in SGC-7901 cells.

关键词

Keywords

references

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Up-regulation of Let-7a Expression Induces Gastric Carcinoma Cell Apoptosis In Vitro

DOI：

摘要

Abstract

关键词

Keywords

references