RNA INTERFERENCE OF ANNEXIN II GENE IN PC3 CELLS BY USING SMALL INTERFERENCE RNA SYNTHESIZED WITH IN VITRO TRANSCRIPTION

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RNA INTERFERENCE OF ANNEXIN II GENE IN PC3 CELLS BY USING SMALL INTERFERENCE RNA SYNTHESIZED WITH IN VITRO TRANSCRIPTION

中国医学科学杂志（英文版） 2007年0卷第1期页码：33-37

Updated：2024-04-10

- RNA INTERFERENCE OF ANNEXIN II GENE IN PC3 CELLS BY USING SMALL INTERFERENCE RNA SYNTHESIZED WITH IN VITRO TRANSCRIPTION
- Affiliations：
  
  Ya-wei Yuan1, Ai-min Sun1, Ying Lui1, Long-hua Chen1, and Banerjee A.G21Department of Radiotherapy, Nanfang Hospital of Nanfang Medical University,Guangzhou 5105152Department of Biochemistry and Molecular Biology, University of Nebraska Medical Center,NE,Omaha,USA,68198-7835
- Author bio：
- Funds：
- DOI：
  中图分类号： R346
- 纸质出版日期：2007
- Accepted：
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RNA INTERFERENCE OF ANNEXIN II GENE IN PC3 CELLS BY USING SMALL INTERFERENCE RNA SYNTHESIZED WITH IN VITRO TRANSCRIPTION[J]. 中国医学科学杂志（英文版）, 2007,0(1):33-37.

RNA INTERFERENCE OF ANNEXIN II GENE IN PC3 CELLS BY USING SMALL INTERFERENCE RNA SYNTHESIZED WITH IN VITRO TRANSCRIPTION[J]. Chinese medical sciences journal, 2007, 0(1): 33-37.
RNA INTERFERENCE OF ANNEXIN II GENE IN PC3 CELLS BY USING SMALL INTERFERENCE RNA SYNTHESIZED WITH IN VITRO TRANSCRIPTION[J]. 中国医学科学杂志（英文版）, 2007,0(1):33-37. DOI：

RNA INTERFERENCE OF ANNEXIN II GENE IN PC3 CELLS BY USING SMALL INTERFERENCE RNA SYNTHESIZED WITH IN VITRO TRANSCRIPTION[J]. Chinese medical sciences journal, 2007, 0(1): 33-37. DOI：

摘要

Abstract

Objective To silence annexin II gene expression by using small interference RNA （siRNA） in prostate cancer cell line PC3. Methods For in vitro transcription

four sequences of 29-nucleotide DNA template oligonucleotides were designed

and one pair of the sequences were complementary to annexin II gene. The other pair was negative control. The 8 nucleotides at the 3’ end of each oligonucleotide were complementary to the T7 Promoter Primer. The sense and antisense siRNA templates were transcribed by T7 RNA polymerase and the resulting RNA transcripts were hybridized to create dsRNA. The siRNA was transfected into prostate cancer cell PC3. For assaying the efficiency of siRNA

confocal microscopy

Northern blotting

and Western blotting were employed to examine the expression of annexin II protein and its mRNA. 3H thymidine was used to measure DNA synthesis. Results The siRNA sequence specific to annexin II gene was capable of inhibiting the expression of annexin II protein and its mRNA. And cellular DNA synthesis was significantly reduced in siRNA transfected cells. Conclusions The protocol for the synthesis of siRNA by T7 RNA polymerase is feasible. Annexin II might be involved in DNA synthesis.

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