HPV 6b L1VIRUS-LIKE PARTICLES ELICIT HUMORAL IMMUNITY IN MICE

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HPV 6b L1VIRUS-LIKE PARTICLES ELICIT HUMORAL IMMUNITY IN MICE

中国医学科学杂志（英文版） 2003年0卷第3期页码：185-188

Updated：2024-04-10

- HPV 6b L1VIRUS-LIKE PARTICLES ELICIT HUMORAL IMMUNITY IN MICE
- Affiliations：
  
  1. Liu Yuehua ,1 ,Liu Wenjun 2 ,Liu Xiaosong 2 and Ian H.Frazer1 Department of Dermatology,PUMC Hospital,CAMS &PUMC,Beijing 2 Center for Immunology and Cancer Research100730,University of Queensland,Qld41 02,Australia
  2. PUMC Hospital
  3. CAMS &PUMC Beijing,100730
  4. Center for Immunology and Cancer Research
  5. University of Queensland Qld41 02
  6. ,China
- Author bio：
- Funds：
- DOI：
  中图分类号： R392
- 纸质出版日期：2003
- Accepted：
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HPV 6b L1VIRUS-LIKE PARTICLES ELICIT HUMORAL IMMUNITY IN MICE[J]. 中国医学科学杂志（英文版）, 2003,0(3):185-188.

HPV 6b L1VIRUS-LIKE PARTICLES ELICIT HUMORAL IMMUNITY IN MICE[J]. Chinese medical sciences journal, 2003, 0(3): 185-188.
HPV 6b L1VIRUS-LIKE PARTICLES ELICIT HUMORAL IMMUNITY IN MICE[J]. 中国医学科学杂志（英文版）, 2003,0(3):185-188. DOI：

HPV 6b L1VIRUS-LIKE PARTICLES ELICIT HUMORAL IMMUNITY IN MICE[J]. Chinese medical sciences journal, 2003, 0(3): 185-188. DOI：

摘要

Abstract

Objective.To test whether intramuscular

intranasal

intrarectal and intravagina l administration of HPV6b L 1 virus-like particlescould induce immune response in mice and to as sess whether intra-muscular and mucosal vaccination against HPV is feasible.Me thods.HPV6b L1proteins self-assembled into VLPs in Sf-9cell in vitro.Mic e were immunized on day0and21with50ìg HPV6b L1VLPs intramuscularly

int ranasally

intrarectally and intravagi-nally respectively.Sera were collected for testing IgG titer after a further7days and3months respec-tively.Results .After immunizations

all mice developed significant anti-HPV6b L1antibody titers in serum by7days after the second immunization.The titer of the serum I gG antibody against HPV6b L1VLPs in the intramuscularly immunized group was h igher than that in the intranasally

intrarectally and intravaginally immunized groups respectively

indicating that both muscular and mucosal administration of HPV6b L1VLPs can stimulate a systemic HPV-specific antibody response.Sera of the mice in the in-tramuscularly immunized group still maintained a high tit er of the serum IgG antibody against HPV6b L1VLPs 3months after the immunizat ion.Conclusion.The results demonstrated that the HPV6b L1VLPs maintain stro ng antigenicity.Immu-nization with HPV6b L1VLPs via intramuscular and mucos al routes

without adjuvant

can elicit spe-cific antibody in sera.These fin dings suggest that the VLPs are able to induce protective antibodies.

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关联资源

CONSTRUCTION AND IMMUNOGENICITY OF HUMAN PAPILLOMAVIRUS TYPE 6B L1 RECOMBINANT PLASMID