DIFFERENTIATION AND MALIGNANT SUPPRESSION INDUCED BY MOUSE ERYTHROID DIFFERENTIATION AND DENUCLEATION FACTOR ON MOUSE ERYTHROLEUKEMIA CELLS

韩代书; 赵青; 葛晔华; 周建平; 马静; 陈克铨; 薛社普

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DIFFERENTIATION AND MALIGNANT SUPPRESSION INDUCED BY MOUSE ERYTHROID DIFFERENTIATION AND DENUCLEATION FACTOR ON MOUSE ERYTHROLEUKEMIA CELLS

中国医学科学杂志（英文版） 2002年第4期页码：199-203

Updated：2024-04-10

- DIFFERENTIATION AND MALIGNANT SUPPRESSION INDUCED BY MOUSE ERYTHROID DIFFERENTIATION AND DENUCLEATION FACTOR ON MOUSE ERYTHROLEUKEMIA CELLS
- Affiliations：
- Author bio：
- Funds：
  
  This work supported by the National Natural Sciences Foundation of China（39670364）;This work was originally published in Acta Academiae Medicinae Sinicae（2001;23: 32-35）in Chinese.
- DOI：
  中图分类号： R733.7
- 纸质出版日期：2002
- Accepted：
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[1]韩代书,,,,,,,,,,赵青,,,,,,,,,,葛晔华,,,,,,,,,,周建平,,,,,,,,,,马静,,,,,,,,,,陈克铨,,,,,,,,,,薛社普.DIFFERENTIATION AND MALIGNANT SUPPRESSION INDUCED BY MOUSE ERYTHROID DIFFERENTIATION AND DENUCLEATION FACTOR ON MOUSE ERYTHROLEUKEMIA CELLS[J].Chinese Medical Sciences Journal,2002(04):199-203.

DIFFERENTIATION AND MALIGNANT SUPPRESSION INDUCED BY MOUSE ERYTHROID DIFFERENTIATION AND DENUCLEATION FACTOR ON MOUSE ERYTHROLEUKEMIA CELLS^△,＃[J]. Chinese medical sciences journal, 2002, (4): 199-203.
[1]韩代书,,,,,,,,,,赵青,,,,,,,,,,葛晔华,,,,,,,,,,周建平,,,,,,,,,,马静,,,,,,,,,,陈克铨,,,,,,,,,,薛社普.DIFFERENTIATION AND MALIGNANT SUPPRESSION INDUCED BY MOUSE ERYTHROID DIFFERENTIATION AND DENUCLEATION FACTOR ON MOUSE ERYTHROLEUKEMIA CELLS[J].Chinese Medical Sciences Journal,2002(04):199-203. DOI：

DIFFERENTIATION AND MALIGNANT SUPPRESSION INDUCED BY MOUSE ERYTHROID DIFFERENTIATION AND DENUCLEATION FACTOR ON MOUSE ERYTHROLEUKEMIA CELLS^△,＃[J]. Chinese medical sciences journal, 2002, (4): 199-203. DOI：

摘要

<正>To investigate the roles of mouse erythroid differentiation and denucleation factor （MEDDF）

a novel factor cloned in our laboratory recently

in erylhroid terminal differentiation.Methods. Mouse erythroleukemia （MEL） cells were transfected with eukaryotie expression plasmid pcD-NA-MEDDF. Then we investigated the changes on characteristics of cell growth by analyzing cells growth rate

mitotie index and colony-forming rate in semi-solid medium. The expressions of c-mye and β-globin genes were analysed by semi-quantitative RT-PCR.Results. MEL cells transfected with pcDNA-MEDDF showed significant lower growth rate

mitotic index

and colony-forming rate in semi-solid medium （ P<0. 01）. The percentage of benzidine-positive cells was 32. 8%after transfection. The expression of β-globin in cells transfected with pcDNA-MEDDF was 3. 43 times higherthan that of control （MEL transfected with blank vector

pcDNA3. 1）

and the expression of c-mye decreased by66. 3% .Conclusions. MEDDF can induce differentiation of

Abstract

Objective. To investigate the roles of mouse erythroid differentiation and denucleation factor （MKI）DF)

a novel factor cloned in our laboratory recently

in erylhroid terminal differentiation.Method. Mouse erythroleukemia （MEL） cells were transfected with eukaryotie expression plasinid pcl)-NA-MEDDF. Then we investigated the changes on characteristics of cell growth by analyzing cells growth rate

mitotie index and colony-forming rate in semi-solid medium. The expressions of c-mye and p-globin genes were analysed by semi-quantitative RT-PCR.Results. MEL cells transfected with pcDNA-MEDDF showed significant lower growth rate

mitolic index

and colony-forming rate in semi-solid medium （ P<0. 01）. The percentage of benzidine-positive cells was 32. 8%after transfection. The expression of β-globin in cells trarisfected with pcDNA-MEDDF was 3. 43 times higherthan that of control （MEL transfected with blank vector

pcDNA3. 1）

and the expression of c-rnyc decreased by66. 3% .Conclusions. MEDDF can induce differentiation of MEL cell and suppress its malignancy.

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