PROTECTIVE EFFECTS OF MELATONIN ON CULTURED RAT CARDIOMYOCYTES DAMAGED BY H<sub>2</sub>O<sub>2</sub>

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PROTECTIVE EFFECTS OF MELATONIN ON CULTURED RAT CARDIOMYOCYTES DAMAGED BY H₂O₂

中国医学科学杂志（英文版） 2003年0卷第4期页码：260-264

Updated：2024-04-10

- PROTECTIVE EFFECTS OF MELATONIN ON CULTURED RAT CARDIOMYOCYTES DAMAGED BY H₂O₂
- Affiliations：
  
  1. Li Yuan 1,,Zheng Yansong 2 ,Gong Weiqin 1 and Wang Xiaoming 1 1 Department of Geriatrics,Xijing Hospital,Fourth Military Medical University,Xi ’an710032 2 Department of Geriatrics,Chinese PLA General Hospital,Beijing,100853
  2. Xijing Hospital
  3. Fourth Military Medical University
  4. Xi ’an,710032
  5. Chinese PLA General Hospital
  6. ,China
- Author bio：
- Funds：
- DOI：
  中图分类号： R96
- 纸质出版日期：2003
- Accepted：
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PROTECTIVE EFFECTS OF MELATONIN ON CULTURED RAT CARDIOMYOCYTES DAMAGED BY H₂O₂[J]. 中国医学科学杂志（英文版）, 2003,0(4):260-264.

PROTECTIVE EFFECTS OF MELATONIN ON CULTURED RAT CARDIOMYOCYTES DAMAGED BY H₂O₂[J]. Chinese medical sciences journal, 2003, 0(4): 260-264.
PROTECTIVE EFFECTS OF MELATONIN ON CULTURED RAT CARDIOMYOCYTES DAMAGED BY H₂O₂[J]. 中国医学科学杂志（英文版）, 2003,0(4):260-264. DOI：

PROTECTIVE EFFECTS OF MELATONIN ON CULTURED RAT CARDIOMYOCYTES DAMAGED BY H₂O₂[J]. Chinese medical sciences journal, 2003, 0(4): 260-264. DOI：

摘要

Abstract

Objective.To investigate the protective effects of melatonin（MT ）on cardiomyocytes against the ox-idative injury induced by H 2 O 2 .Methods.Ventricular myocytes were isolated from neonatal rats and cultured for3～5days.Cells were divided into4groups:control group

H 2 O 2 （100μmol/L H 2 O 2 ）group

MT group（100μmol/L MT ）and MT preconditioning group（100μmol/L MT+100μmol/L H 2 O 2 ）.The fluorescent probe

DCFH?DA

was used to detect intracellular levels of reactive oxygen species（ROS）and another fluorescent probe

Fluo-3?AM

was used to detect[Ca 2+ ] i by using a laser scanning confocal microscopy（LSCM）.The malondi-aldehyde（MDA）content in cardiomyocytes was determined by measuring thiobarbituric acid?reactive sub-stances to monitor lipid peroxidation.The activity of cytoplasmic enzyme lactate dehydrogenase（LDH）that was released into the culture media was assayed to indicate alternation in the integrity of the cellular membrane.Trypan blue exclusion was used to detect the cell viability.Results.Compared with the control group

intracellular ROS

[Ca 2+ ] i

MDA content

LDH leakage and cell death were significantly elevated when cells were treated by100μmol/L H 2 O 2 for60minutes（P<0.01）.However

those changes were significantly attenuated in MT preconditioning group.Conclusion.MT has very good antioxidant effect and can protect the cardiomyocytes against H 2 O 2 -in-duced injury.

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PROTECTIVE EFFECTS OF MELATONIN ON CULTURED RAT CARDIOMYOCYTES DAMAGED BY H2O2

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PROTECTIVE EFFECTS OF MELATONIN ON CULTURED RAT CARDIOMYOCYTES DAMAGED BY H₂O₂