THE COEXPRESSION OF THE preS1(1-42) AND THE CORE(1-144)ANTIGEN OF HBV IN  E.coli

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THE COEXPRESSION OF THE preS1(1-42) AND THE CORE(1-144)ANTIGEN OF HBV IN E.coli

中国医学科学杂志（英文版） 2002年0卷第2期页码：68-72

Updated：2024-04-10

- THE COEXPRESSION OF THE preS1(1-42) AND THE CORE(1-144)ANTIGEN OF HBV IN E.coli
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  中图分类号： R392.1
- 纸质出版日期：2002
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THE COEXPRESSION OF THE preS1(1-42) AND THE CORE(1-144)ANTIGEN OF HBV IN E.coli[J]. 中国医学科学杂志（英文版）, 2002,0(2):68-72.

THE COEXPRESSION OF THE preS1（1-42）AND THE CORE（1-144）ANTIGEN OF HBV IN E.coli[J]. Chinese medical sciences journal, 2002, 0(2): 68-72.
THE COEXPRESSION OF THE preS1(1-42) AND THE CORE(1-144)ANTIGEN OF HBV IN E.coli[J]. 中国医学科学杂志（英文版）, 2002,0(2):68-72. DOI：

THE COEXPRESSION OF THE preS1（1-42）AND THE CORE（1-144）ANTIGEN OF HBV IN E.coli[J]. Chinese medical sciences journal, 2002, 0(2): 68-72. DOI：

摘要

Abstract

Objective.To study the therapeutic T cell vaccine for the treatment of chronic hepatitis B by improving the cellular immunization of HBsAg vaccine with the coexpression of the preS1（1-42）and the Core（1-144）anti-gen of HBV in E.coli.Methods.The genes of HBcAg （1-144）and preS1（1-42）were amplified and fused by PCR.This fused gene was inserted in the prokaryotic expression vector pET-11d and expre ssed in E.coli.Results.It was showed by SDS-PAGE that the pr otein molecular weight of the coexpr ession product was about 20kD

20%of all bacteria prote in.The monoclonal antibodies again st core and preS1antibody could re-act with this fused protein by Western-blot technique respectively.The fused gene was verified by sequencin g.Under the immune electron microscop y

this fused protein is typical particles of HBcAg but in an aggregated form.Conclusion.The results might aid for studying T c ell immunotherapeutic vaccine for c hronic hepatitis B.

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