Aim. To study the effect of kanglemycin C （KC） on production and gene transcription of lymphokins. Methods.Cell proliferation and lymphokin activities were quantified with MTT colorimetry and ELISA

and gene transcriptions of lymphokins semi quantified with RT PCR. Results.Suppression of KC on proliferation of enriched T and B cell respectively mediated by Con A and LPS was declined by addition of exogenous IL 1

IL 2

and IL 6. KC 80 nmol/L markedly inhibited IL 2 and IL 6 production and mRNA transcription of incubated mouse splenocytes induced by Con A. Additionally

KC had some suppression on IL 1β and IL 6 productions of peritoneal macrophage stimulated by LPS （5μg/mL）

whereas cyclosporine （CS） had not. [WT5”BX]Conclusion. [WT5”BZ]Immunosuppression of KC came true partially through the decrease of IL 1β

2 and 6 productions

especially of IL 2. However

CS′s immunosuppression was mainly through the decrease of IL 2 procduction.