Objective. So far

it has not been established a satisfactory method for early diagnosis and studying on epidemiology for leprosy

we want to develop a molecular biological method for solving this point. Materials and methods. Based on the M. leprae gene coding groEL

65kD and 16S rRNA

three polymerase chain reactions were developed by using plikaytis’

Woods’ and Pattyn’s procedures. It was optimized that the experimental parameters for each PCR

and a comparative study on practivity among three PCRs was also conducted for practical purpose.Results and conclusion. For detecting infection with M. leprae

all of PCRs established by us were highly sensitive and specific

but for practicl purpose

the woods’ PCR optimized by us ought to be chosen firstly.